质粒名称: | mEos3.2-N1 |
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出品公司: | Addgene |
目录编号: | 54525 |
存储实验室: | Michael Davidson, Tao Xu |
载体骨架基本信息 |
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载体名称: | mEos3.2-N1 |
载体出品公司: | -- |
载体抗性: | 卡那霉素 |
空载体大小: | 4750 bp |
完整质粒大小: | -- |
载体修饰: | -- |
载体类型: | 哺乳细胞表达 |
筛选标记: | 新霉素(G418) |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | -- |
物种来源: | -- |
插入大小: | -- |
基因突变: | -- |
GenBank编号 | -- |
启动子 | CMV |
标签/融合蛋白: | mEos3.2(主干网上的C终端) |
克隆方法: | -- |
5' 克隆位点: | -- |
3' 克隆位点: | -- |
5' 测序引物 | CMV-F |
3' 测序引物 | SV40pA-R |
文献引用方法 |
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材料和方法部分: | mEos3.2-N1 was a gift from Michael Davidson & Tao Xu (Addgene plasmid # 54525 ; http://n2t.net/addgene:54525 ; RRID:Addgene_54525) |
参考文献部分: | Rational design of true monomeric and bright photoactivatable fluorescent proteins. Zhang M, Chang H, Zhang Y, Yu J, Wu L, Ji W, Chen J, Liu B, Lu J, Liu Y, Zhang J, Xu P, Xu T. Nat Methods. 2012 May 13;9(7):727-9. doi: 10.1038/nmeth.2021. 10.1038/nmeth.2021 PubMed 22581370 |
买家导航
mEos3.2N1载体基本信息
mEos3.2N1载体图谱质粒图谱和多克隆位点信息
mEos3.2N1载体简介
mEos3.2N1载体序列
LOCUS mEos3.2-N1 4693 bp ds-DNA circular SYN 06-AUG-2018 DEFINITION Localization: N1 Cloning Vector, Excitation: 507 / 572, Emission: 516 / 580. ACCESSION . VERSION . KEYWORDS mEos3.2-N1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4693) TITLE Michael Davidson Empty Backbones REFERENCE 2 (bases 1 to 4693) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4693 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 68..371 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 372..575 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 526..546 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" misc_feature 598..678 /label=MCS /note="multiple cloning site" CDS 686..1366 /codon_start=1 /product="improved monomeric variant of green-to-red photoswitchable fluorescent protein EosFP (Zhang et al., 2012)" /label=mEos3.2 /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD NARR" polyA_signal 1488..1609 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1525..1544) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1579..1598 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(1616..2071) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1753..1774) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 1965..1984 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2098..2202 /gene="bla" /label=AmpR promoter promoter 2204..2561 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2227..2247) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2412..2547 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2474..2493 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2596..3390 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2650..2669) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3260..3279 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(3578..3597) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 3622..3669 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 3998..4586 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4487..4506 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg 61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc 121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt 181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc 241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 601 agcgctaccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg 661 ggcccgggat ccaccggtcg ccaccatgag tgcgattaag ccagacatga agatcaaact 721 ccgtatggaa ggcaacgtaa acgggcacca ctttgtgatc gacggagatg gtacaggcaa 781 gccttttgag ggaaaacaga gtatggatct tgaagtcaaa gagggcggac ctctgccttt 841 tgcctttgat atcctgacca ctgcattcca ttacggcaac agggtattcg ccaaatatcc 901 agacaacata caagactatt ttaagcagtc gtttcctaag gggtattcgt gggaacgaag 961 cttgactttc gaagacgggg gcatttgcaa cgccagaaac gacataacaa tggaagggga 1021 cactttctat aataaagttc gattttatgg taccaacttt cccgccaatg gtccagttat 1081 gcagaagaag acgctgaaat gggagccctc cactgagaaa atgtatgtgc gtgatggagt 1141 gctgacgggt gatattgaga tggctttgtt gcttgaagga aatgcccatt accgatgtga 1201 cttcagaact acttacaaag ctaaggagaa gggtgtcaag ttaccaggcg cccactttgt 1261 ggaccactgc attgagattt taagccatga caaagattac aacaaggtta agctgtatga 1321 gcatgctgtt gctcattctg gattgcctga caatgccaga cgataagcgg ccgcgactct 1381 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac 1441 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg 1501 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 1561 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt 1621 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt 1681 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg 1741 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc 1801 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca 1861 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga 1921 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa 1981 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc 2041 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc 2101 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 2161 taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc 2221 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa 2281 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc 2341 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc 2401 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct 2461 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga 2521 agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga 2581 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg 2641 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc 2701 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt 2761 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt 2821 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc 2881 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc 2941 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac 3001 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag 3061 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag 3121 gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat 3181 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg 3241 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa 3301 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc 3361 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc 3421 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt 3481 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca 3541 tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac 3601 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt 3661 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga 3721 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt 3781 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag 3841 gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 3901 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 3961 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 4021 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 4081 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 4141 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 4201 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 4261 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 4321 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 4381 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 4441 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 4501 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 4561 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 4621 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata 4681 accgtattac cgc //
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