| 质粒名称: | BB3cK_pGAP_23*_pLAT1_Cas9 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 104908 |
| 存储实验室: | Brigitte Gasser |
|
载体骨架基本信息 |
|
| 载体名称: | Episomal ARS/CEN Backbone |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 酵母表达,CRISPR |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | HH - FS23 linker - HDV |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BbsI(未破坏) |
| 3' 克隆位点: | BbsI (未破坏) |
| 5' 测序引物 | pGAP fw |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | BB3cK_pGAP_23*_pLAT1_Cas9 was a gift from Brigitte Gasser (Addgene plasmid # 104908 ; http://n2t.net/addgene:104908 ; RRID:Addgene_104908) |
| 参考文献部分: | CRISPR/Cas9-Mediated Homology-Directed Genome Editing in Pichia pastoris. Gassler T, Heistinger L, Mattanovich D, Gasser B, Prielhofer R. Methods Mol Biol. 2019;1923:211-225. doi: 10.1007/978-1-4939-9024-5_9. 10.1007/978-1-4939-9024-5_9 PubMed 30737742 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS BB3cK_pGAP_23*_pLAT1_Cas9 9104 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR
products for episomal expression in P. pastoris and selection on
G418.
ACCESSION .
VERSION .
KEYWORDS BB3cK_pGAP_23*_pLAT1_Cas9
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9104)
AUTHORS Gassler T, Heistinger L, Mattanovich D, Gasser B, Prielhofer R.
TITLE CRISPR/Cas9-mediated homology directed genome editing in Pichia
pastoris
JOURNAL (2018) In Press
REFERENCE 2 (bases 1 to 9104)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9104
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin complement(70..658)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(150..169)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
promoter 745..1221
/label=GAP promoter
/note="promoter for Pichia pastoris
glyceraldehyde-3-phosphate dehydrogenase"
CDS 2765..6868
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 6881..6901
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
terminator 6973..7220
/gene="S. cerevisiae CYC1"
/label=CYC1 terminator
/note="transcription terminator for CYC1"
primer_bind complement(6989..7007)
/label=CYC1
/note="CYC1 transcription termination signal, reverse
primer"
gene 7240..8596
/label=kanMX
/note="yeast selectable marker conferring kanamycin
resistance (Wach et al., 1994)"
promoter 7240..7583
/label=TEF promoter
/note="Ashbya gossypii TEF promoter"
CDS 7584..8393
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind complement(7651..7670)
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
terminator 8399..8596
/label=TEF terminator
/note="Ashbya gossypii TEF terminator"
misc_feature 8608..7
/label=CEN/ARS
/note="S. cerevisiae CEN6 centromere fused to an
autonomously replicating sequence"
ORIGIN
1 agcgatcgcg ctgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
61 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
121 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
181 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
241 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
301 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
361 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
421 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
481 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
541 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
601 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
661 agatcctttg atcttttcta cggggtggag cgcaaaaaac cccgcccctg acagggcggg
721 gttttttcgc gatcggagga tccttttttg tagaaatgtc ttggtgtcct cgtccaatca
781 ggtagccatc tctgaaatat ctggctccgt tgcaactccg aacgacctgc tggcaacgta
841 aaattctccg gggtaaaact taaatgtgga gtaatggaac cagaaacgtc tcttcccttc
901 tctctccttc caccgcccgt taccgtccct aggaaatttt actctgctgg agagcttctt
961 ctacggcccc cttgcagcaa tgctcttccc agcattacgt tgcgggtaaa acggaggtcg
1021 tgtacccgac ctagcagccc agggatggaa aagtcccggc cgtcgctggc aataatagcg
1081 ggcggacgca tgtcatgaga ttattggaaa ccaccagaat cgaatataaa aggcgaacac
1141 ctttcccaat tttggtttct cctgacccaa agactttaaa tttaatttat ttgtccctat
1201 ttcaatcaat tgaacaacta tcaaaacacc atggcgtctt cggatccctc gaggaagacg
1261 cgcttttagt gtacatctga taatatagta ctaccacgta tgataatgta gagaataggc
1321 ttccttgtcg agtgtgtttg cagttttctt gagtttcaag gtttaaatgc tggtatatta
1381 gttcatcgaa ggtttcagcc aatagcacct taaatcaatc aaactaattc gactcttacg
1441 aaagagccta ctgtgtttag tatcgaagtc gtttaccttt catgttgaat agcttcctct
1501 ctgaccctaa catttcaaga tcctcctaaa gttacccgga ttgtgaaatt ctaatgatcc
1561 acctgcccaa tgcatttttt ctttattcag tttacctttt ttacctaata tacgagcttg
1621 ttaaagtaag tggcactgca atactaggct tattgttgat attatgatga atcgttttca
1681 caaacttgat ttcctgtgaa ctcaccatgt actaaggaaa aaaacatgca tcaccatctg
1741 aatatttgac cgctccgggg agggaagaat atgaaccacg attgaaagac tttgggtttg
1801 ccgattctaa aacacttact gaacagaaaa gaagtgaatt attagagagt ttatgcaacg
1861 gagatcatga gcttagaaag aacgtaggct gggcaacaac atccatttct gcctgcgata
1921 tttccagtgc aatgctaaag ccacataata taggcaatta caacttgaat gagcaggctc
1981 atgatgtcac catggaactg atagataaag tcatccaaaa aggtgtcaat ttacggcacg
2041 tctacgtaga tactgtcgga cctccagcat cataccaaaa gaaactgcaa caaaggtttc
2101 ccgcactgct gatcaccgta tctaaaaaag cagatgccat ctatccatgt gtttcaagtg
2161 cttcagtttg cgccaaggtc actagagacg tctgtttgag aacctcaaat ggggaggaga
2221 acctattaga ttgtgggtct ggatatccct cggacccaaa tactaagaaa tggctagccc
2281 agtctataca tccattgttt ggctggagtc gtgatgtcag gttttcgtgg cagacggcca
2341 aagattgtct tgagagacac aactcaatca aagttgtgtt tggcgaagag gagcctaccc
2401 cggttaattt cggtgatctg gggttgagca aacagaagcc cgactttaag gatttagaaa
2461 tcagtaccag ttggtgggga cattaattgg tggaggagag ccaatattct caactgttcg
2521 taggaccata accccccact cgcaccagaa atactcattg gcgctgacaa ctagtttgag
2581 ccgcttcaat tgcggcaagt ccccagtttt gtttggtact cccctctcgc ctcgcgtgtt
2641 tttgttcctc cgaattgtgc atcaccctgt cacatccgta caccatcgga acactctgct
2701 cctcctcctt tctcctctta attgaacgta tatctaatta ccctaataac aagtatcttc
2761 aaccatggac aagaagtact ccattgggct cgatatcggc acaaacagcg tcggttgggc
2821 cgtcattacg gacgagtaca aggtgccgag caaaaaattc aaagttctgg gcaataccga
2881 tcgccacagc ataaagaaga acctcattgg cgccctcctg ttcgactccg gggagacggc
2941 cgaagccacg cggctcaaaa gaacagcacg gcgcagatat acccgcagaa agaatcggat
3001 ctgctacctg caggagatct ttagtaatga gatggctaag gtggatgact ctttcttcca
3061 taggctggag gagtcctttt tggtggagga ggataaaaag cacgagcgcc acccaatctt
3121 tggcaatatc gtggacgagg tggcgtacca tgaaaagtac ccaaccatat atcatctgag
3181 gaagaagctt gtagacagta ctgataaggc tgacttgcgg ttgatctatc tcgcgctggc
3241 gcatatgatc aaatttcggg gacacttcct catcgagggg gacctgaacc cagacaacag
3301 cgatgtcgac aaactcttta tccaactggt tcagacttac aatcagcttt tcgaagagaa
3361 cccgatcaac gcatccggag ttgacgccaa agcaatcctg agcgctaggc tgtccaaatc
3421 ccggcggctc gaaaacctca tcgcacagct ccctggggag aagaagaacg gcctgtttgg
3481 taatcttatc gccctgtcac tcgggctgac ccccaacttt aaatctaact tcgacctggc
3541 cgaagatgcc aagcttcaac tgagcaaaga cacctacgat gatgatctcg acaatctgct
3601 ggcccagatc ggcgaccagt acgcagacct ttttttggcg gcaaagaacc tgtcagacgc
3661 cattctgctg agtgatattc tgcgagtgaa cacggagatc accaaagctc cgctgagcgc
3721 tagtatgatc aagcgctatg atgagcacca ccaagacttg actttgctga aggcccttgt
3781 cagacagcaa ctgcctgaga agtacaagga aattttcttc gatcagtcta aaaatggcta
3841 cgccggatac attgacggcg gagcaagcca ggaggaattt tacaaattta ttaagcccat
3901 cttggaaaaa atggacggca ccgaggagct gctggtaaag cttaacagag aagatctgtt
3961 gcgcaaacag cgcactttcg acaatggaag catcccccac cagattcacc tgggcgaact
4021 gcacgctatc ctcaggcggc aagaggattt ctaccccttt ttgaaagata acagggaaaa
4081 gattgagaaa atcctcacat ttcggatacc ctactatgta ggccccctcg cccggggaaa
4141 ttccagattc gcgtggatga ctcgcaaatc agaagagact atcactccct ggaacttcga
4201 ggaagtcgtg gataaggggg cctctgccca gtccttcatc gaaaggatga ctaactttga
4261 taaaaatctg cctaacgaaa aggtgcttcc taaacactct ctgctgtacg agtacttcac
4321 agtttataac gagctcacca aggtcaaata cgtcacagaa gggatgagaa agccagcatt
4381 cctgtctgga gagcagaaga aagctatcgt ggacctcctc ttcaagacga accggaaagt
4441 taccgtgaaa cagctcaaag aggactattt caaaaagatt gaatgtttcg actctgttga
4501 aatcagcgga gtggaggatc gcttcaacgc atccctggga acgtatcacg atctcctgaa
4561 aatcattaaa gacaaggact tcctggacaa tgaggagaac gaggacattc ttgaggacat
4621 tgtcctcacc cttacgttgt ttgaagatag ggagatgatt gaagaacgct tgaaaactta
4681 cgctcatctc ttcgacgaca aagtcatgaa acagctcaag aggcgccgat atacaggatg
4741 ggggcggctg tcaagaaaac tgatcaatgg gatccgagac aagcagagtg gaaagacaat
4801 cctggatttt cttaagtccg atggatttgc caaccggaac ttcatgcagt tgatccatga
4861 tgactctctc acctttaagg aggacatcca gaaagcacaa gtttctggcc agggggacag
4921 ccttcacgag cacatcgcta atcttgcagg tagcccagct atcaaaaagg gaatactgca
4981 gaccgttaag gtcgtggatg aactcgtcaa agtaatggga aggcataagc ccgagaatat
5041 cgttatcgag atggcccgag agaaccaaac tacccagaag ggacagaaga acagtaggga
5101 aaggatgaag aggattgaag agggtataaa agaactgggg tcccaaatcc ttaaggaaca
5161 cccagttgaa aacacccagc ttcagaatga gaagctctac ctgtactacc tgcagaacgg
5221 cagggacatg tacgtggatc aggaactgga catcaatcgg ctctccgact acgacgtgga
5281 tcatatcgtg ccccagtctt ttctcaaaga tgattctatt gataataaag tgttgacaag
5341 atccgataaa aatagaggga agagtgataa cgtcccctca gaagaagttg tcaagaaaat
5401 gaaaaattat tggcggcagc tgctgaacgc caaactgatc acacaacgga agttcgataa
5461 tctgactaag gctgaacgag gtggcctgtc tgagttggat aaagccggct tcatcaaaag
5521 gcagcttgtt gagacacgcc agatcaccaa gcacgtggcc caaattctcg attcacgcat
5581 gaacaccaag tacgatgaaa atgacaaact gattcgagag gtgaaagtta ttactctgaa
5641 gtctaagctc gtctcagatt tcagaaagga ctttcagttt tataaggtga gagagatcaa
5701 caattaccac catgcgcatg atgcctacct gaatgcagtg gtaggcactg cacttatcaa
5761 aaaatatccc aagcttgaat ctgaatttgt ttacggagac tataaagtgt acgatgttag
5821 gaaaatgatc gcaaagtctg agcaggaaat aggcaaggcc accgctaagt acttctttta
5881 cagcaatatt atgaattttt tcaagaccga gattacactg gccaatggag agattcggaa
5941 gcgaccactt atcgaaacaa acggagaaac aggagaaatc gtgtgggaca agggtaggga
6001 tttcgcgaca gtccggaagg tcctgtccat gccgcaggtg aacatcgtta aaaagaccga
6061 agtacagacc ggaggcttct ccaaggaaag tatcctcccg aaaaggaaca gcgacaagct
6121 gatcgcacgc aaaaaagatt gggaccccaa gaaatacggc ggattcgatt ctcctacagt
6181 cgcttacagt gtactggttg tggccaaagt ggagaaaggg aagtctaaaa aactcaaaag
6241 cgtcaaggaa ctgctgggca tcacaatcat ggagcgatca agcttcgaaa aaaaccccat
6301 cgactttctc gaggcgaaag gatataaaga ggtcaaaaaa gacctcatca ttaagcttcc
6361 caagtactct ctctttgagc ttgaaaacgg ccggaaacga atgctcgcta gtgcgggcga
6421 gctgcagaaa ggtaacgagc tggcactgcc ctctaaatac gttaatttct tgtatctggc
6481 cagccactat gaaaagctca aagggtcccc cgaagataat gagcagaagc agctgttcgt
6541 ggaacaacac aaacactacc ttgatgagat catcgagcaa ataagcgaat tctccaaaag
6601 agtgatcctc gccgacgcta acctcgataa ggtgctttct gcttacaata agcacaggga
6661 taagcccatc agggagcagg cagaaaacat tatccacttg tttactctga ccaacttggg
6721 cgcgcctgca gccttcaagt acttcgacac caccatagac agaaagcggt acacctctac
6781 aaaggaggtc ctggacgcca cactgattca tcagtcaatt acggggctct atgaaacaag
6841 aatcgacctc tctcagctcg gtggagacag cagggctgac cccaagaaga agaggaaggt
6901 gtgagcttcg tccgacggcg gcccgacggg tccgaggcct cggagatccg tccccctttt
6961 cctttgtcga tatcatgtaa ttagttatgt cacgcttaca ttcacgccct ccccccacat
7021 ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct atttattttt
7081 ttatagttat gttagtatta agaacgttat ttatatttca aatttttctt ttttttctgt
7141 acagacgcgt gtacgcatgt aacattatac tgaaaacctt gcttgagaag gttttgggac
7201 gctcgaaggc tttaatttgc aagctggcgc taattcatgg acatggaggc ccagaatacc
7261 ctccttgaca gtcttgacgt gcgcagctca ggggcatgat gtgactgtcg cccgtacatt
7321 tagcccatac atccccatgt ataatcattt gcatccatac attttgatgg ccgcacggcg
7381 cgaagcaaaa attacggctc ctcgctgcag acgtgcgagc agggaaacgc tcccctcaca
7441 gacgcgttga attgtcccca cgccgcgccc ctgtagagaa atataaaagg ttaggatttg
7501 ccactgaggt tcttctttca tatacttcct tttaaaatct tgctaggata cagttctcac
7561 atcacatccg aacataaaca accatgggta aggaaaagac tcacgtttcg aggccgcgat
7621 taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc
7681 aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga
7741 aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc
7801 tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat
7861 ggttactcac cactgcgatc cccggcaaaa cagcattcca ggtattagaa gaatatcctg
7921 attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc
7981 ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac
8041 gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg
8101 ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat tcagtcgtca
8161 ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta
8221 ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact
8281 gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata
8341 atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagtac
8401 tgacaataaa aagattcttg ttttcaagaa cttgtcattt gtatagtttt tttatattgt
8461 agttgttcta ttttaatcaa atgttagcgt gatttatatt ttttttcgcc tcgacatcat
8521 ctgcccagat gcgaagttaa gtgcgcagaa agtaatatca tgcgtcaatc gtatgtgaat
8581 gctggtcgct atactggctt ccttttcatc acgtgctata aaaataatta taatttaaat
8641 tttttaatat aaatatataa attaaaaata gaaagtaaaa aaagaaatta aagaaaaaat
8701 agtttttgtt ttccgaagat gtaaaagact ctagggggat cgccaacaaa tactaccttt
8761 tatcttgctc ttcctgctct caggtattaa tgccgaattg tttcatcttg tctgtgtaga
8821 acaccacaca cgaaaatcct gtgattttac attttactta tcgttaatcg aatgtatatc
8881 tatttaatct gcttttcttg tctaataaat atatatgtaa agtacgcttt ttgttgaaat
8941 tttttaaacc tttgtttatt tttttttctt cattccgtaa ctcttctacc ttctttattt
9001 actttctaaa atccaaatac aaaacataaa aataaataaa cacagagtaa attcccaaat
9061 tattccatca ttaaaagata cgaggcgcgt gtaagttaca ggca
//
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