| 质粒名称: | L4440 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 1654 |
| 存储实验室: | Andrew Fire |
|
载体骨架基本信息 |
|
| 载体名称: | -- |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 2790 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 蠕虫表达,RNAi |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | T7p, T7p, lacZN, OriF1>>, OriF1<< |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | L4440 was a gift from Vladislav Verkhusha (爱迪基因 plasmid # 1654) |
| 参考文献部分: |
Near-infrared fluorescent proteins for multicolor in vivo imaging. Shcherbakova DM, Verkhusha VV. Nat Methods. 2013 Jun 16. doi: 10.1038/nmeth.2521. 10.1038/nmeth.2521 PubMed 23770755 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS L4440 2790 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS L4440
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2790)
AUTHORS Fire A et al
TITLE Fire Lab C. Elegans Vector Kit 1999
REFERENCE 2 (bases 1 to 2790)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..2790
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(368..1228)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 991..1010
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1229..1333)
/gene="bla"
/label=AmpR promoter
rep_origin complement(1359..1814)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1496..1517)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 1708..1727
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 1941..1963
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 1955..1972
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 1956..1972
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 1982..2000
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 2025..2041
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind 2026..2042
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2112..2128)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2112..2128)
/label=pBluescriptSK
/note="For pBluescript vector"
promoter complement(2186..2204)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(2228..2245)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2399..197)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2479..2498)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
61 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
121 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
181 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
241 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
301 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
361 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
421 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
481 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
541 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
601 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
661 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
721 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
781 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
841 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
901 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
961 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
1021 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
1081 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
1141 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
1201 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
1261 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
1321 taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt
1381 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
1441 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
1501 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
1561 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
1621 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
1681 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
1741 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
1801 gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
1861 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
1921 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc
1981 gtaatacgac tcactatagg gcgaattggg taccgggccc cccctcgagg tcgacggtat
2041 cgataagctt gatatcgaat tcctgcagcc cgggggatcc acgcgtcacg tggctagcca
2101 tggaaccggt ggatccacta gttctagagc ggccgccacc gcggtggagc tcgaattcat
2161 cgatgatatc agatctgccg gtctccctat agtgagtcgt attaatttcg ataagccagg
2221 ttgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
2281 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
2341 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
2401 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
2461 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
2521 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
2581 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
2641 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
2701 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
2761 acaggattag cagagcgagg tatgtaggcg
//
