| 质粒名称: | MMK |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 64121 |
| 存储实验室: | Linzhao Cheng |
|
载体骨架基本信息 |
|
| 载体名称: | pCEP4 |
| 完整质粒大小: | 11271 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Top10 |
|
插入基因信息 |
|
| 插入基因名称: | MYC-2A-KLF4 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 2859 bp |
| GenBank编号 | NM_004235.4 |
| 启动子: | SFFV |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | AsiSI（如果已销毁，则未知） |
| 3’克隆位点: | PmeI（如果已销毁，则未知） |
| 5’测序引物 | 5-AGGCATGGAAAAATACCAAA, or 5-TAACCAATCAGCCTGCTTCT |
|
文献引用方法 |
|
| 材料和方法部分: | MMK was a gift from Linzhao Cheng (Addgene plasmid # 64121 ; http://n2t.net/addgene:64121 ; RRID:Addgene_64121) |
| 参考文献部分: | A Facile Method to Establish Human Induced Pluripotent Stem Cells From Adult Blood Cells Under Feeder-Free and Xeno-Free Culture Conditions: A Clinically Compliant Approach. Chou BK, Gu H, Gao Y, Dowey SN, Wang Y, Shi J, Li Y, Ye Z, Cheng T, Cheng L. Stem Cells Transl Med. 2015 Mar 5. pii: sctm.2014-0214. 10.5966/sctm.2014-0214 PubMed 25742692 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS MMK 10701 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION a modified episomal (EBNA1/OriP) vector expressing human Myc and
KLF4 genes.
ACCESSION .
VERSION .
KEYWORDS MMK
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10701)
AUTHORS Chou BK, Gu H, Gao Y, Dowey SN, Wang Y, Shi J, Li Y, Ye Z, Cheng T,
Cheng L
TITLE A Facile Method to Establish Human Induced Pluripotent Stem Cells
From Adult Blood Cells Under Feeder-Free and Xeno-Free Culture
Conditions: A Clinically Compliant Approach.
JOURNAL Stem Cells Transl Med. 2015 Mar 5. pii: sctm.2014-0214.
PUBMED 25742692
REFERENCE 2 (bases 1 to 10701)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10701
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 2084..3873
/label=oriP
/note="Epstein-Barr virus oriP replication origin (Yates et
al., 2000)"
primer_bind 4436..4458
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
polyA_signal 4601..4735
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4638..4657)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 4692..4711
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
CDS 4832..4843
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind 5241..5261
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5331..5354)
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS complement(5373..5402)
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS complement(6858..6917)
/codon_start=1
/product="2A peptide from equine rhinitis A virus
polyprotein"
/label=E2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="QCTNYALLKLAGDVESNPGP"
CDS complement(6918..8234)
/codon_start=1
/product="human c-Myc proto-oncogene"
/label=c-Myc
/translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
promoter complement(8293..8700)
/label=SFFV promoter
/note="spleen focus-forming virus long terminal repeat
(LTR) promoter"
rep_origin complement(8883..9471)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8963..8982)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9642..10502)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10265..10284
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10503..10607)
/gene="bla"
/label=AmpR promoter
primer_bind 10675..10693
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 cttcaagaat tctcatgttt gacagcttat catcgataag ctgatcctca caggccgcac
61 ccagcttttc ttccgttgcc ccagtagcat ctctgtctgg tgaccttgaa gaggaagagg
121 aggggtcccg agaatcccca tccctaccgt ccagcaaaaa gggggacgag gaatttgagg
181 cctggcttga ggctcaggac gcaaatcttg aggatgttca gcgggagttt tccgggctgc
241 gagtaattgg tgatgaggac gaggatggtt cggaggatgg ggaattttca gacctggatc
301 tgtctgacag cgaccatgaa ggggatgagg gtgggggggc tgttggaggg ggcaggagtc
361 tgcactccct gtattcactg agcgtcgtct aataaagatg tctattgatc tcttttagtg
421 tgaatcatgt ctgacgaggg gccaggtaca ggacctggaa atggcctagg agagaaggga
481 gacacatctg gaccagaagg ctccggcggc agtggacctc aaagaagagg gggtgataac
541 catggacgag gacggggaag aggacgagga cgaggaggcg gaagaccagg agccccgggc
601 ggctcaggat cagggccaag acatagagat ggtgtccgga gaccccaaaa acgtccaagt
661 tgcattggct gcaaagggac ccacggtgga acaggagcag gagcaggagc gggaggggca
721 ggagcaggag gggcaggagc aggaggaggg gcaggagcag gaggaggggc aggaggggca
781 ggaggggcag gagcaggagg aggggcagga gcaggagggg caggagcagg aggtggaggc
841 cggggtcgag gaggcagtgg aggccggggt cgaggaggta gtggaggccg gggtcgagga
901 ggtagtggag gccgccgggg tagaggacgt gaaagagcca gggggggaag tcgtgaaaga
961 gccaggggga gaggtcgtgg acgtggagaa aagaggccca ggagtcccag tagtcagtca
1021 tcatcatccg ggtctccacc gcgcaggccc cctccaggta gaaggccatt tttccaccct
1081 gtaggggaag ccgattattt tgaataccac caagaaggtg gcccagatgg tgagcctgac
1141 gtgcccccgg gagcgataga gcagggcccc gcagatgacc caggagaagg cccaagcact
1201 ggaccccggg gtcagggtga tggaggcagg cgcaaaaaag gagggtggtt tggaaagcat
1261 cgtggtcaag gaggttccaa cccgaaattt gagaacattg cagaaggttt aagagctctc
1321 ctggctagga gtcacgtaga aaggactacc gacgaaggaa cttgggtcgc cggtgtgttc
1381 gtatatggag gtagtaagac ctccctttac aacctaaggc gaggaactgc ccttgctatt
1441 ccacaatgtc gtcttacacc attgagtcgt ctcccctttg gaatggcccc tggacccggc
1501 ccacaacctg gcccgctaag ggagtccatt gtctgttatt tcatggtctt tttacaaact
1561 catatatttg ctgaggtttt gaaggatgcg attaaggacc ttgttatgac aaagcccgct
1621 cctacctgca atatcagggt gactgtgtgc agctttgacg atggagtaga tttgcctccc
1681 tggtttccac ctatggtgga aggggctgcc gcggagggtg atgacggaga tgacggagat
1741 gaaggaggtg atggagatga gggtgaggaa gggcaggagt gatgtaactt gttaggagac
1801 gccctcaatc gtattaaaag ccgtgtattc ccccgcacta aagaataaat ccccagtaga
1861 catcatgcgt gctgttggtg tatttctggc catctgtctt gtcaccattt tcgtcctccc
1921 aacatggggc aattgggcat acccatgttg tcacgtcact cagctccgcg ctcaacacct
1981 tctcgcgttg gaaaacatta gcgacattta cctggtgagc aatcagacat gcgacggctt
2041 tagcctggcc tccttaaatt cacctaagaa tgggagcaac cagcaggaaa aggacaagca
2101 gcgaaaattc acgccccctt gggaggtggc ggcatatgca aaggatagca ctcccactct
2161 actactgggt atcatatgct gactgtatat gcatgaggat agcatatgct acccggatac
2221 agattaggat agcatatact acccagatat agattaggat agcatatgct acccagatat
2281 agattaggat agcctatgct acccagatat aaattaggat agcatatact acccagatat
2341 agattaggat agcatatgct acccagatat agattaggat agcctatgct acccagatat
2401 agattaggat agcatatgct acccagatat agattaggat agcatatgct atccagatat
2461 ttgggtagta tatgctaccc agatataaat taggatagca tatactaccc taatctctat
2521 taggatagca tatgctaccc ggatacagat taggatagca tatactaccc agatatagat
2581 taggatagca tatgctaccc agatatagat taggatagcc tatgctaccc agatataaat
2641 taggatagca tatactaccc agatatagat taggatagca tatgctaccc agatatagat
2701 taggatagcc tatgctaccc agatatagat taggatagca tatgctatcc agatatttgg
2761 gtagtatatg ctacccatgg caacattagc ccaccgtgct ctcagcgacc tcgtgaatat
2821 gaggaccaac aaccctgtgc ttggcgctca ggcgcaagtg tgtgtaattt gtcctccaga
2881 tcgcagcaat cgcgccccta tcttggcccg cccacctact tatgcaggta ttccccgggg
2941 tgccattagt ggttttgtgg gcaagtggtt tgaccgcagt ggttagcggg gttacaatca
3001 gccaagttat tacaccctta ttttacagtc caaaaccgca gggcggcgtg tgggggctga
3061 cgcgtgcccc cactccacaa tttcaaaaaa aagagtggcc acttgtcttt gtttatgggc
3121 cccattggcg tggagccccg tttaattttc gggggtgtta gagacaacca gtggagtccg
3181 ctgctgtcgg cgtccactct ctttcccctt gttacaaata gagtgtaaca acatggttca
3241 cctgtcttgg tccctgcctg ggacacatct taataacccc agtatcatat tgcactagga
3301 ttatgtgttg cccatagcca taaattcgtg tgagatggac atccagtctt tacggcttgt
3361 ccccacccca tggatttcta ttgttaaaga tattcagaat gtttcattcc tacactagta
3421 tttattgccc aaggggtttg tgagggttat attggtgtca tagcacaatg ccaccactga
3481 accccccgtc caaattttat tctgggggcg tcacctgaaa ccttgttttc gagcacctca
3541 catacacctt actgttcaca actcagcagt tattctatta gctaaacgaa ggagaatgaa
3601 gaagcaggcg aagattcagg agagttcact gcccgctcct tgatcttcag ccactgccct
3661 tgtgactaaa atggttcact accctcgtgg aatcctgacc ccatgtaaat aaaaccgtga
3721 cagctcatgg ggtgggagat atcgctgttc cttaggaccc ttttactaac cctaattcga
3781 tagcatatgc ttcccgttgg gtaacatatg ctattgaatt agggttagtc tggatagtat
3841 atactactac ccgggaagca tatgctaccc gtttagggtt aacaaggggg ccttataaac
3901 actattgcta atgccctctt gagggtccgc ttatcggtag ctacacaggc ccctctgatt
3961 gacgttggtg tagcctcccg tagtcttcct gggcccctgg gaggtacatg tcccccagca
4021 ttggtgtaag agcttcagcc aagagttaca cataaaggca atgttgtgtt gcagtccaca
4081 gactgcaaag tctgctccag gatgaaagcc actcagtgtt ggcaaatgtg cacatccatt
4141 tataaggatg tcaactacag tcagagaacc cctttgtgtt tggtcccccc ccgtgtcaca
4201 tgtggaacag ggcccagttg gcaagttgta ccaaccaact gaagggatta catgcactgc
4261 cccgcgaaga aggggcagag atgtcgtagt caggtttagt tcgtccgggg cggggatcga
4321 tcctctagta gagtcgacct catggctgcg ccccgacacc cgccaacacc cgctgacgcg
4381 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
4441 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gccggatcat
4501 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
4561 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
4621 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
4681 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgccc
4741 aaagggagat ccgactcgtc tgagggcgaa ggcgaagacg cggaagaggc cgcagagccg
4801 gcagcaggcc gcgggaagga aggtccgctg gattgagggc cgaagggacg tagcagaagg
4861 acgtcccgcg cagaatccag gtggcaacac aggcgagcag ccatggaaag gacgtcagct
4921 tccccgacaa caccacggaa ttgtcagtgc ccaacagccg agcccctgtc cagcagcggg
4981 caaggcaggc ggcgatgagt tccgccgtgg caatagggag ggggaaagcg aaagtcccgg
5041 aaaggagctg acaggtggtg gcaatgcccc aaccagtggg ggttgcgtca gcaaacacag
5101 tgcacaccac gccacgttgc ctgacaacgg gccacaactc ctcataaaga gacagcaacc
5161 aggatttata caaggaggag aaaatgaaag ccatacggga agcaatagca tgatacaaag
5221 gcattaaagc agcgtatcca catagcgtaa aaggagcaac atagttaaga ataccagtca
5281 atctttcaca aattttgtaa tccagaggtt gattgtcgac gcgtttaaac cttatcgtcg
5341 tcatccttgt aatccaggat atcatttgct gccagatcct cttctgagat gagtttctgc
5401 tcgagcggcc gcgtacgcgt aaaatgcctc ttcatgtgta aggcgaggtg gtccgacctg
5461 gaaaatgctc ggtcgcattt ttggcactgg aacgggcggt gccccgtgtg tttacggtag
5521 tgcctggtca gttcatctga gcgggcgaat ttccatccac agccgtccca gtcacagtgg
5581 taaggtttct cacctgtgtg ggttcgcagg tgtgccttga gatgggaact ctttgtgtag
5641 gttttgccgc agcccgcgta atcacaagtg tgggtggcgg tccttttccg gggccacgat
5701 cgtcttcccc tctttggctt gggctcctct ggcatgcagg aaccgggtgg catgagctct
5761 tggtaatgga gcggcgggac ttgcggctgc atctgatcgg gcaggaagga tgggtaattg
5821 ggccccgggt ggggatggaa gccgggagga agcggcaggg cagggtgaca gtccctgctg
5881 ctcagcactt cctcaagacc cagggtcggg gtagtcctgc tggggagctg ccgccccagg
5941 gggaagtcgt gtgcagccgg ccggtggcca ttgctgagag ggggtccagc gcccaagtgg
6001 gtgcacgaag agaccgcctc ctgcttgatc ttggggcacg tgcgcggcgg cccgccgttg
6061 tagggcgcca ccaccaccgg gtggctgccg tcagggctgc ctttgctgac gctgatgacc
6121 gacgggctgc cgtactcgct gccaggggcg ctcagcgacg ccttcagcac gaacttgccc
6181 atcagcccgc cacctggcgg ctgcggctgc tgcggcggaa tgtacaccgg gtccaattct
6241 ggccgcagga gctcggccac gaagccgccc gaggggctca cgtcgttgat gtccgccagg
6301 ttgaagggag ccgtcggagg gggagcggac tccctgccat agaggaggcc tccgcccgtg
6361 ccgcccggcg ccacgcccgg gtcgttcccg gcccggatcg gataggtgaa gctgcaggtg
6421 gagggcgcgc tggcagggcc gctgctcgac ggcgacgacg aagaggaggc tgacgctgac
6481 gaggacacgg tggcggccac tgactccgga ggatgggtca gcgaattgga gagaataaag
6541 tccaggtcca ggagatcgtt gaactcctcg gtctctctcc gaggtagggg cgccaggttg
6601 ctaccgccgc aagccgcacc ggctccgccg ctctccaggt ctgtggccac ggtcgccgcc
6661 gccaggtcat aggggcggcc gggaagcact gggggaagtc gcttcatgtg ggagagctcc
6721 tcccgccagc ggttattcgg ggcacctgct tgacgcagtg tcttctccct tcccgccggg
6781 ccagacgcga acgtggagaa agatgggagc agcgcgtcgc tgacagccat gtcagactcg
6841 ccaggtggct gcctcatagg accggggtta ctttcaacat cgccagcgag tttcaacaaa
6901 gcgtagttag tacattgcgc acaagagttc cgtagctgtt caagtttgtg tttcaactgt
6961 tctcgtcgtt tccgcaacaa gtcctcttca gaaatgagct tttgctcctc tgcttggacg
7021 gacaggatgt atgctgtggc ttttttaagg ataactacct tgggggcctt ttcattgttt
7081 tccaactccg ggatctggtc acgcagggca aaaaagctcc gttttagctc gttcctcctc
7141 tggcgctcca agacgttgtg tgttcgcctc ttgacattct cctcggtgtc cgaggacctg
7201 gggctggtgc attttcggtt gttgctgatc tgtctcagga ctctgacact gtccaacttg
7261 accctcttgg cagcaggata gtccttccga gtggagggag gcgctgcgta gttgtgctga
7321 tgtgtggaga cgtggcacct cttgaggacc agtgggctgt gaggaggttt gctgtggcct
7381 ccagcagaag gtgatccaga ctctgacctt ttgccaggag cctgcctctt ttccacagaa
7441 acaacatcga tttcttcctc atcttcttgt tcctcctcag agtcgctgct ggtggtgggc
7501 ggtgtctcct catggagcac caggggctcg gggctgccct gcggggagga ctccgtcgag
7561 gagagcagag aatccgagga cggagagaag gcgctggagt cttgcgaggc gcaggacttg
7621 ggcgagctgc tgtcgttgag agggtagggg aagaccaccg aggggtcgat gcactctgag
7681 gcggcggcgc tcagatcctg caggtacaag ctggaggtgg agcagacgct gtggccgcgg
7741 gcggggttcg ggctgccgct gtctttgcgc gcagcctggt aggaggccag cttctctgag
7801 acgagcttgg cggcggccga gaagccgctc cacatacagt cctggatgat gatgtttttg
7861 atgaaggtct cgtcgtccgg gtcgcagatg aaactctggt tcaccatgtc tcctcccagc
7921 agctcggtca ccatctccag ctggtcggcc gtggagaagc tcccgccacc gccgtcgttg
7981 tctccccgaa gggagaaggg tgtgaccgca acgtaggagg gcgagcagag cccggagcgg
8041 cggctagggg acaggggcgg ggtgggcagc agctcgaatt tcttccagat atcctcgctg
8101 ggcgccgggg gctgcagctc gctctgctgc tgctgctggt agaagttctc ctcctcgtcg
8161 cagtagaaat acggctgcac cgagtcgtag tcgaggtcat agttcctgtt ggtgaagcta
8221 acgttgaggg gcatcgtcgc gggaggctgc tggttttcca ctacccgaaa aaaatccatg
8281 gcgatcgcgg tacccgggcg actcagtctg tcggaggact ggcgcgccga gtgaggggtt
8341 gtgagctctt ttatagagct cgggaagcag aagcgcgcga acagaagcga gaagcaggct
8401 gattggttaa ttcaaataag gcgcagggtc atttcaggtc cttgggggag cctggaaaca
8461 tctgatgggt cttaagaaac tgctgagggt tgggccatat ctggggacca tctgttcttg
8521 gcctcgggcc ggggccgaaa ctgcggtgac catctgttct tggccccggg ccggggccga
8581 aactgctcac cgcagatatc ctgtttggcc caacgttagc tattttcatg tacccgccct
8641 tgatctgaac ttctctattc ttggtttggt atttttccat gccttgcaaa atggcgttac
8701 tgcagctagc tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc
8761 ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat
8821 cagggacagc ttcaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
8881 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
8941 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
9001 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
9061 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
9121 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
9181 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
9241 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
9301 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
9361 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
9421 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
9481 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
9541 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
9601 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
9661 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
9721 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
9781 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
9841 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
9901 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca
9961 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
10021 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
10081 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
10141 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
10201 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca
10261 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
10321 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
10381 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
10441 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
10501 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
10561 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
10621 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
10681 cgtatcacga ggccctttcg t
//
