| 质粒名称: | STOP-eGFP-ROSA26TV |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 11739 |
| 存储实验室: | Klaus Rajewsky |
|
载体骨架基本信息 |
|
| 载体名称: | pBSII KS |
| 载体出品公司: | Stratagene |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 2900 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | Cre/Lox ; /STOP |
| 筛选标记: | 新霉素（选用G418）；白喉毒素A |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | STOP-eGFP-ROSA26TV |
| 物种来源: | 来自酵母大肠杆菌、转座子Tn5、棒状杆菌（水母）的DNA |
| 插入大小: | 11500 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | SnaBI(未破坏) |
| 3' 克隆位点: | SacI (未破坏) |
| 5' 测序引物 | T7/M13 |
| 3' 测序引物 | T7/M13 |
|
文献引用方法 |
|
| 材料和方法部分: | STOP-eGFP-ROSA26TV was a gift from Klaus Rajewsky (Addgene plasmid # 11739 ; http://n2t.net/addgene:11739 ; RRID:Addgene_11739) |
| 参考文献部分: | Canonical NF-kappaB activity, dispensable for B cell development, replaces BAFF-receptor signals and promotes B cell proliferation upon activation. Sasaki Y, Derudder E, Hobeika E, Pelanda R, Reth M, Rajewsky K, Schmidt-Supprian M. Immunity. 2006 Jun . 24(6):729-39. 10.1016/j.immuni.2006.04.005 PubMed 16782029 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS STOP-eGFP-ROSA26TV 14367 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS STOP-eGFP-ROSA26TV
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 14367)
AUTHORS Sasaki Y, Derudder E, Hobeika E, Pelanda R, Reth M, Rajewsky K,
Schmidt-Supprian M
TITLE Canonical NF-kappaB activity, dispensable for B cell development,
replaces BAFF-receptor signals and promotes B cell proliferation
upon activation.
JOURNAL Immunity. 2006 Jun . 24(6):729-39.
PUBMED 16782029
REFERENCE 2 (bases 1 to 14367)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..14367
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 5..1086
/label=Rosa26 left arm
/note="left homology arm from intron 1 of the mouse Rosa26
gene (Soriano, 1999)"
misc_feature 1093..1196
/label=Ad SA
/note="adenovirus major late transcript splice acceptor
(Friedrich and Soriano, 1991)"
protein_bind 1207..1240
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
polyA_signal 1247..1295
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
CDS complement(1302..2105)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(1413..1432)
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind 2023..2042
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
intron 2919..2984
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 3114..3134
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 3559..3693
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3596..3615)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 3650..3669
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
protein_bind 3713..3746
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
protein_bind 3779..3826
/label=FRT
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
misc_feature 3865..4416
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(4009..4026)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 4236..4255
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 4428..5147
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(4473..4494)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(4734..4753)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 5081..5102
/label=EGFP-C
/note="EGFP, forward primer"
protein_bind 5172..5219
/label=FRT
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
primer_bind complement(5244..5260)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5244..5260)
/label=pBluescriptSK
/note="For pBluescript vector"
polyA_signal 5279..5503
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
promoter 9897..10387
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind 10320..10339
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 10414..10983
/codon_start=1
/gene="Corynebacterium diphtheriae tox"
/product="diphteria toxin Fragment A"
/label=DTA
/translation="DDVVILLIFCMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYDD
DWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKELG
LSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEIN
FETRGKRGQDAMYEYMAQACAGNRVRR"
intron 11060..11125
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
polyA_signal 11199..11423
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(11522..11542)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(11522..11540)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(11561..11577)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(11561..11577)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(11574..11596)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 11585..11601
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(11609..11639)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 11654..11675
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(11792..11809)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(11963..12551)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(12043..12062)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(12722..13582)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 13345..13364
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(13583..13687)
/gene="bla"
/label=AmpR promoter
rep_origin complement(13713..14168)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(13850..13871)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 14062..14081
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 14295..14317
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 14309..14326
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 14310..14326
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 14336..14355
/label=T7
/note="T7 promoter, forward primer"
promoter 14336..14354
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 ccccgcggca ggccctccga gcgtggtgga gccgttctgt gagacagccg ggtacgagtc
61 gtgacgctgg aaggggcaag cgggtggtgg gcaggaatgc ggtccgccct gcagcaaccg
121 gagggggagg gagaagggag cggaaaagtc tccaccggac gcggccatgg ctcggggggg
181 ggggggcagc ggaggacgct tccggccgac gtctcgtcgc tgattggctt ttttcctccc
241 gccgtgtgtg aaaacacaaa tggcgtgttt tggttggcgt aaggcgcctg tcagttaacg
301 gcagccggag tgcgcagccg ccggcagcct cgctctgccc actgggtggg gcgggaggta
361 ggtggggtga ggcgagctga cgtgcgggcg cggtcggcct ctggcggggc gggggagggg
421 agggagggtc agcgaaagta gctcgcgcgc gagcggccgc ccaccctccc cttcctctgg
481 gggagtcgtt ttacccgccg ccggccgggc ctcgtcgtct gattggctct cggggcccag
541 aaaactggcc cttgccattg gctcgtgttc gtgcaagttg agtccatccg ccggccagcg
601 ggggcggcga ggaggcgctc ccaggttccg gccctcccct cggccccgcg ccgcagagtc
661 tggccgcgcg cccctgcgca acgtggcagg aagcgcgcgc tgggggcggg gacgggcagt
721 agggctgagc ggctgcgggg cgggtgcaag cacgtttccg acttgagttg cctcaagagg
781 ggcgtgctga gccagacctc catcgcgcac tccggggagt ggagggaagg agcgagggct
841 cagttgggct gttttggagg caggaagcac ttgctctccc aaagtcgctc tgagttgtta
901 tcagtaaggg agctgcagtg gagtaggcgg ggagaaggcc gcacccttct ccggaggggg
961 gaggggagtg ttgcaatacc tttctgggag ttctctgctg cctcctggct tctgaggacc
1021 gccctgggcc tgggagaatc ccttgccccc tcttcccctc gtgatctgca actccagtct
1081 ttctagcatc tgtagggcgc agtagtccag ggtttccttg atgatgtcat acttatcctg
1141 tccctttttt ttccacagct cgcggttgag gacaaactct tcgcggtctt tccagtggtt
1201 aattaaataa cttcgtatag catacattat acgaagttat ggatccgaac aaacgaccca
1261 acacccgtgc gttttattct gtctttttat tgccgatccc ctcagaagaa ctcgtcaaga
1321 aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag
1381 cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc
1441 tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt
1501 tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg
1561 ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg
1621 tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga
1681 tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt
1741 gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc
1801 cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca
1861 gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt
1921 tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac
1981 agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat
2041 agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatggccgat
2101 cccatattgg ctgcagggtc gctcggtgtt cgaggccaca cgcgtcacct taatatgcga
2161 agtggacctg ggaccgcgcc gccccgactg catctgcgtg ttcgaattcg ccaatgacaa
2221 gacgctgggc ggggtttgct cgacattggg tggaaacatt ccaggcctgg gtggagaggc
2281 tttttgcttc ctcttgcaaa accacactgc tcgacattgg gtggaaacat tccaggcctg
2341 ggtggagagg ctttttgctt cctcttgcaa aaccacactg ctcgactagt gattaaagtc
2401 gactcgggga caccaaatat ggcgatctcg gccttttcgt ttcttggagc tgggacatgt
2461 ttgccatcga tccatctacc accagaacgg ccgttagatc tgctgccacc gttgtttcca
2521 ccgaagaaac caccgttgcc gtaaccacca cgacggttgt tgctaaagaa gctgccaccg
2581 ccacggccac cgttgtagcc gccgttgttg ttattgtagt tgctcatgtt atttctggca
2641 cttcttggtt ttcctcttaa gtgaggagga acataaccat tctcgttgtt gtcgttgatg
2701 cttaaatttt gcacttgttc gctcagttca gccataatat gaaatgcttt tcttgttgtt
2761 cttacggaat accacttgcc acctatcacc acaactaact ttttcccgtt cctccatctc
2821 ttttatattt tttttctcga gggatctttg tgaaggaacc ttacttctgt ggtgtgacat
2881 aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg
2941 tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa
3001 ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag
3061 aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa
3121 agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc
3181 atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag
3241 ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata
3301 acagttataa tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta
3361 ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata
3421 aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta
3481 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
3541 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
3601 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
3661 aaactcatca atgtatctta tcatgtctgg atctgacatg gtaagtaagc ttataacttc
3721 gtatagcata cattatacga agttatcggc gcgccgcggc cgcgatcaat tcggtaccga
3781 agttcctatt ccgaagttcc tattctctag aaagtatagg aacttcctcg agggtacccc
3841 aattccgccc cccccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
3901 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
3961 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
4021 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
4081 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
4141 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
4201 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
4261 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
4321 gcacatgctt tacatgtgtt tagtcgaggt taaaaaacgt ctaggccccc cgaaccacgg
4381 ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacaaccatg gtgagcaagg
4441 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
4501 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
4561 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
4621 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
4681 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
4741 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
4801 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
4861 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
4921 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
4981 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
5041 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
5101 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaatcc gggatccgga
5161 gagctcccaa cgaagttcct attccgaagt tcctattctc tagaaagtat aggaacttcc
5221 tcgaggttgg atgcagcccg ggggatccac tagttctaga gcggccgatc agcctcgact
5281 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
5341 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
5401 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
5461 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
5521 accagctggg gctcgagatc cactagttct agcctcgagg ctagagcggc cgccgcggat
5581 atcgaattcg ctagaagatg ggcgggagtc ttctgggcag gcttaaaggc taacctggtg
5641 tgtgggcgtt gtcctgcagg ggaattgaac aggtgtaaaa ttggagggac aagacttccc
5701 acagattttc ggttttgtcg ggaagttttt taataggggc aaataggaaa atggaggata
5761 ggagtcatct ggggtttatg cagcaaaact acaggtatat tgcttgtatc cgcctcggag
5821 atttccatga ggagataaag acatgtcacc cgagtttata ctctcctgct tagatcctac
5881 tacagtatga aatacagtgt gcgaggtaga ctatgtaagc agatttaatc attttaaaga
5941 gcccagtact tcatatccat ttctcccgct ccttctgcag ccttatcaaa aggtatttag
6001 aacactcatt ttagccccat tttcatttat tatactggct tatccaaccc ctagacagag
6061 cattggcatt ttccctttcc tgatcttaga agtctgatga ctcatgaaac cagacagatt
6121 agttacatac accacaaatc gaggctgtag ctggggcctc aacactgcag ttcttttata
6181 actccttagt acactttttg ttgatccttt gccttgatcc ttaattttca gtgtctatca
6241 cctctcccgt caggtggtgt tccacatttg ggcctattct cagtccaggg agttttacaa
6301 caatagatgt attgagaatc caacctaaag cttaactttc cactcccatg aatgcctctc
6361 tcctttttct ccattataac tgagctatac cattaatggt ttcaggtgga tgtctcctcc
6421 cccaatatac ctgatgtatc tacatattgc caggctgata ttttaagaca taaaggtata
6481 tttcattatt gagccacatg gtattgatta ctgctactaa aattttgtca ttgtacacat
6541 ctgtaaaagg tggttccttt tggaatgcaa agttcaggtg tttgttgtct ttcctgacct
6601 aaggtcttgt gagcttgtat tttttctatt taagcagtgc tttctcttgg actggcttga
6661 ctcatggcat tctacacgtt attgctggtc taaatgtgat tttgccaagc ttcttcagga
6721 cctataattt tgcttgactt gtagccaaac acaagtaaaa tgattaagca acaaatgtat
6781 ttgtgaagct tggtttttag gttgttgtgt tgtgtgtgct tgtgctctat aataatacta
6841 tccaggggct ggagaggtgg ctcggagttc aagagcacag actgctcttc cagaagtcct
6901 gagttcaatt cccagcaacc acatggtggc tcacaaccat ctgtaatggg atctgatgcc
6961 ctcttctggt gtgtctgaag accacaagtg tattcacatt aaataaataa tcctccttct
7021 tcttcttttt ttttttttaa agagaatctg tctccagtag aattactgaa gtaatgaaat
7081 actttgtgtt tgttccaata tggagccaat aatcaaatac tcttagcact ggaaatgtac
7141 caaggaacta ttttatttaa gtgactgtgg acagaggagc cataactgca gacttgtggg
7201 atacagaaga ccaatgcaga cttaatgtct tttctcttac actaagcaat aaagaaataa
7261 aaattgaact tctagtatcc tatttgttaa actgctagct ttactaactt ttgtgcttca
7321 tctatacaaa gctgaaagct aagtctgcag ccattactaa acatgaaagc aagtaatgat
7381 aattttggat ttcaaaaatg tagggccaga gtttagccag ccagtggtgg tgcttgcctt
7441 tatgccttaa tcccagcact ctggaggcag agacaggcag atctctgagt ttgagcccag
7501 cctggtctac acatcaagtt ctatctagga tagccaggaa tacacacaga aaccctgttg
7561 gggagggggg ctctgagatt tcataaaatt ataattgaag cattccctaa tgagccacta
7621 tggatgtggc taaatccgtc tacctttctg atgagatttg ggtattattt tttctgtctc
7681 tgctgttggt tgggtctttt gacactgtgg gctttcttaa agcctccttc cctgccatgt
7741 ggtctcttgt ttgctactaa cttcccatgg cttaaatggc atggcttttt gccttctaag
7801 ggcagctgct gagtttgcag cctgatttcc agggtggggt tgggaaatct ttcaaacact
7861 aaaattgtcc tttaattttt ttttaaaaaa tgggttatat aataaacctc ataaaatagt
7921 tatgaggagt gaggtggact aatattaatg agtccctccc ctataaaaga gctattaagg
7981 ctttttgtct tatactaact ttttttttaa atgtggtatc tttagaacca agggtcttag
8041 agttttagta tacagaaact gttgcatcgc ttaatcagat tttctagttt caaatccaga
8101 gaatccaaat tcttcacagc caaagtcaaa ttaagaattt ctgactttaa tgttatttgc
8161 tactgtgaat ataaaatgat agcttttcct gaggcagggt ctcactatgt atctctgcct
8221 gatctgcaac aagatatgta gactaaagtt ctgcctgctt ttgtctcctg aatactaagg
8281 ttaaaatgta gtaatacttt tggaacttgc aggtcagatt cttttatagg ggacacacta
8341 agggagcttg ggtgatagtt ggtaaatgtg tttaagtgat gaaaacttga attattatca
8401 ccgcaaccta ctttttaaaa aaaaaagcca ggcctgttag agcatgctaa gggatcccta
8461 ggacttgctg agcacacaag agtagtactt ggcaggctcc tggtgagagc atatttcaaa
8521 aaacaaggca gacaaccaag aaactacagt aaggttacct gtctttaacc atctgcatat
8581 acacagggat attaaaatat tccaaataat atttcattca agttttcccc catcaaattg
8641 ggacatggat ttctccggtg aataggcaga gttggaaact aaacaaatgt tggttttgtg
8701 atttgtgaaa ttgttttcaa gtgatagtta aagcccatga gatacagaac aaagctgcta
8761 tttcgaggtc tcttggttat actcagaagc acttctttgg gtttccctgc actatcctga
8821 tcatgtgcta ggcctcctta ggctgattgt tgttcaaata acttaagttt cctgtcaggt
8881 gatgtcatat gatttcatat atcaaggcaa aacatgttat atatgttaaa catttgactt
8941 aatgtgaaag ttaggtcttt gtgggttttg attttaattt caaaacctga gctaaataag
9001 tcattttaca tgtcttacat ttggtgaatt gtatattgtg gtttgcaggc aagactctct
9061 gacctagtaa ccctcctata gagcactttg ctgggtcaca agtctaggag tcaagcattt
9121 caccttgaag ttgagacgtt ttgttagtgt atactagtta tatgttggag gacatgttta
9181 tccagaagat attcaggact atttttgact gggctaagga attgattctg attagcactg
9241 ttagtgagca ttgagtggcc tttaggcttg aattggagtc acttgtatat ctcaaataat
9301 gctggccttt tttaaaagcc cttgttcttt atcaccctgt tttctacata atttttgttc
9361 aaagaaatac ttgtttggat ctccttttga caacaatagc atgttttcaa gccatatttt
9421 ttttcctttt tttttttttt tttggttttt cgagacaggg tttctctgta tagccctggc
9481 tgtcctggaa ctcactttgt agaccaggct ggcctcgaac tcagaaatcc gcctgcctct
9541 gcctcctgag tgccgggatt aaaggcgtgc accaccacgc ctggctaagt tggatatttt
9601 gtatataact ataaccaata ctaactccac tgggtggatt tttaattcag tcagtagtct
9661 taagtggtct ttattggccc ttattaaaat ctactgttca ctctaacaga ggctgttgga
9721 ctagtggact aagcaacttc ctacggatat actagcagat aagggtcagg gatagaaact
9781 agtctagcgt tttgtatacc taccagctta tactaccttg ttctgataga aatatttagg
9841 acatctagct tatcgatccg tcgacggtat cgataagctt gatatcgaat tctaccgggt
9901 aggggaggcg cttttccaag gcagtctgag catgcgctta gcagccccgc tggcacttgg
9961 cgctacacaa gtggccttgg cctcgcacac attccacatc caccggtagg cgccaaccgg
10021 ctccgttctt tggtggcccc ttcgcgccac cttctctcct cccctagtca ggaagttccc
10081 ccccgccccg cagctcgcgt cgtaggacgt gacaaatgga agtagcacgt ctcactagtc
10141 tcgtcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
10201 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
10261 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
10321 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
10381 cctttcgacc tgcaggtcct cgccatggat cctgatgatg ttgttattct tctaatcttt
10441 tgtatggaaa acttttcttc gtaccacggg actaaacctg gttatgtaga ttccattcaa
10501 aaaggtatac aaaagccaaa atctggtaca caaggaaatt atgacgatga ttggaaaggg
10561 ttttatagta ccgacaataa atacgacgct gcgggatact ctgtagataa tgaaaacccg
10621 ctctctggaa aagctggagg cgtggtcaaa gtgacgtatc caggactgac gaaggttctc
10681 gcactaaaag tggataatgc cgaaactatt aagaaagagt taggtttaag tctcactgaa
10741 ccgttgatgg agcaagtcgg aacggaagag tttatcaaaa ggttcggtga tggtgcttcg
10801 cgtgtagtgc tcagccttcc cttcgctgag gggagttcta gcgttgaata tattaataac
10861 tgggaacagg cgaaagcgtt aagcgtagaa cttgagatta attttgaaac ccgtggaaaa
10921 cgtggccaag atgcgatgta tgagtatatg gctcaagcct gtgcaggaaa tcgtgtcagg
10981 cgatctcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac
11041 agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact
11101 gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg
11161 gtggaatgca gatcctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc
11221 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
11281 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
11341 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
11401 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctcgacctc
11461 gagggggggc ccggtaccgc ggccgcgatc gcctgcaggt acgtagtacc agcttttgtt
11521 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
11581 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
11641 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
11701 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
11761 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
11821 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
11881 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
11941 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
12001 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
12061 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
12121 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
12181 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
12241 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
12301 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
12361 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
12421 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
12481 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
12541 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
12601 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
12661 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
12721 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
12781 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
12841 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
12901 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
12961 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
13021 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
13081 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
13141 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
13201 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
13261 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
13321 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
13381 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
13441 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
13501 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
13561 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
13621 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
13681 ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa
13741 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
13801 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
13861 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
13921 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
13981 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
14041 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
14101 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
14161 gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
14221 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
14281 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata
14341 cgactcacta tagggcgaat tggagct
//
