质粒名称: | Tet-O-FUW-Myt1l |
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出品公司: | 爱迪基因 |
目录编号: | 27152 |
存储实验室: | Marius Wernig |
载体骨架基本信息 |
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载体名称: | TetO-FUW |
载体抗性: | 氨苄霉素 |
空载体大小: | 8373 bp |
完整质粒大小: | 11950 bp |
载体类型: | 哺乳细胞表达,Lentiviral |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | Myt1l |
物种来源: | M. musculus (老鼠) |
插入大小: | 3577 bp |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | EcoR1(未破坏) |
3' 克隆位点: | EcoR1(未破坏) |
5' 测序引物 | LNCX |
3' 测序引物 | WPRE-R |
文献引用方法 |
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材料和方法部分: | Tet-O-FUW-Myt1l was a gift from Marius Wernig (爱迪基因 plasmid # 27152) |
参考文献部分: | Direct conversion of fibroblasts to functional neurons by defined factors. Vierbuchen T, Ostermeier A, Pang ZP, Kokubu Y, Sudhof TC, Wernig M. Nature. 2010 Feb 25. 463(7284):1035-41. 10.1038/nature08797 PubMed 20107439 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Tet-O-FUW-Myt1l 11956 bp ds-DNA circular SYN 10-JAN-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Tet-O-FUW-Myt1l SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11956) AUTHORS Vierbuchen T, Ostermeier A, Pang ZP, Kokubu Y, Sudhof TC, Wernig M TITLE Direct conversion of fibroblasts to functional neurons by defined factors. JOURNAL Nature. 2010 Feb 25. 463(7284):1035-41. PUBMED 20107439 COMMENT This file is created by Vector NTI http://www.biofeng.com/ COMMENT ORIGDB|GenBank COMMENT VNTAUTHORNAME|biofeng.com| COMMENT VNTNAME|Tet-O-FUW-Myt1l| FEATURES Location/Qualifiers source 1..11956 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 9..18 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" misc_feature 3603..4191 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(3656..3676) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(4074..4085) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(4194..4210) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4195..4211) /label=pBluescriptKS /note="For pBluescript vector" LTR 4716..4896 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(4922..4939) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 4928..5152 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 5198..5626 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5285..5304) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5495..5516 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(5635..5655) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 5640..5969 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 5820..5955 /label=SV40 ori /note="SV40 origin of replication" primer_bind 5882..5901 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 6017..6064 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 6083..6457 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 6587..6708 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(6624..6643) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 6678..6697 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(6757..6773) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6757..6773) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6770..6792) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6781..6797 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6805..6835) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6850..6871 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(6988..7005) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7159..7747) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7239..7258) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7918..8778) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8541..8560 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8779..8883) /gene="bla" /label=AmpR promoter primer_bind complement(8958..8977) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 9149..9528 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 9530..9728 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 9683..9703 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 9746..9926 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 9973..10098 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 10591..10824 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." misc_feature 11351..11468 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 11522..11792 /label=tetracycline response element /bound_moiety="tetracycline repressor TetR" /note="contains seven copies of the tetracycline operator tetO" promoter 11825..11862 /label=minimal CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 11859..11883 /label=LNCX /note="Human CMV promoter, forward primer" ORIGIN 1 aattgctagc caccatggac gtggactctg aggagaagcg ccatcgcaca cggtccaaag 61 gggttcgagt tcctgtggag ccagccatac aagagctgtt cagctgtccc actccaggct 121 gcgacggcag tggtcacgtc agtggcaaat atgcacgaca cagaagtgta tatggttgtc 181 ccttggctaa aaaaagaaaa acgcaagata aacagcccca agaacctgct cccaagcgaa 241 aaccatttgc agtaaaagca gatagttcct cagtagacga atgttatgag agtgatggta 301 ctgaagacat ggatgataag gaggaagatg atgatgagga gttctctgaa gacaatgatg 361 agcaagggga tgatgacgac gaagatgagg tggatcggga agacgaggag gagatcgagg 421 aggaagatga tgaagaagat gatgatgatg aagatggtga cgatgtagaa gaggaagaag 481 aggatgatga tgaagaggag gaagaagagg aagaggaaga agaaaatgaa gaccatcaaa 541 tgagttgtac tcgaataatg caggacacag acaaggatga taacaacaat gatgagtatg 601 ataactatga tgaactggta gctaagtcgc tattaaatct tggcaaaatt gctgaggatg 661 cagcataccg agccaggact gaatcagaga tgaacagcaa tacctccaat agtctggagg 721 acgatagtga caaaaacgaa aacctcggtc ggaaaagcga actgagtcta gacttagaca 781 gtgatgttgt tagagaaaca gtggactccc ttaagctgtt agcacaagga catggtgttg 841 tgctatcaga gaatatcagt gacagaagtt atgctgaggg gatgtcacag caggacagta 901 gaaatatgaa ctatgtcatg ctagggaagc ccatgaacaa tggactcatg gagaagatgg 961 tggaggagag tgatgaggaa gtgtgtctaa gtagtctaga gtgcctgagg aaccagtgct 1021 ttgacctggc caggaaactc agcgagacca acccacagga caggagtcag ccacccaaca 1081 tgagtgtgcg ccaacatgtc cggcaagagg acgacttccc tgggaggacg ccagacagga 1141 gctactcgga tatgatgaac cttatgcggc tggaggagca gctcagtccc aggtctagaa 1201 cgttctccag ctgtgccaag gaggatgggt gtcatgagag ggatgatgac accacctcag 1261 tgaactcaga caggtctgag gaagtgtttg acatgaccaa gggcaacctg actctgctag 1321 agaaagccat tgccttggag acagagagag ccaaggccat gcgggagaag atggccatgg 1381 atgctgggag aagggataac ctgagatcct atgaggacca gtctccaaga cagctggctg 1441 gggaagacag aaaatccaaa tccagtgaca gccatgtcaa aaagccatac tatggtaaag 1501 atccctcaag aacagaaaag agagagagca agtgtccaac ccccgggtgt gatggaaccg 1561 gccacgtaac tgggctttac ccgcatcacc gcagtctgtc tggatgcccg cacaaagata 1621 gggtccctcc agaaattctt gccatgcatg aaaatgttct caagtgtccc actccaggct 1681 gcacagggcg agggcatgtg aatagcaaca ggaactcgca cagaagcctc tctggatgcc 1741 ccattgctgc tgcagaaaaa ctggcaaagg cccaagagaa acaccagagc tgtgatgtgt 1801 ccaaatccaa ccaggcctca gaccgagtcc tcaggccaat gtgctttgtc aaacagcttg 1861 agattcctca gtatggctac agaaacaatg ttcccacaac cacaccacgc tccaacctgg 1921 ccaaggagct tgagaaatac tccaagactt cgtttgagta caacagttac gacaaccata 1981 cttatggcaa aagagccata gctcccaagg tgcaaaccag ggacatatcc cccaaaggat 2041 atgacgatgc caagcggtac tgcaagaatg ccagccccag cagcagcacc accagcagct 2101 atgcacctag cagcagcagc aacctcagct gtggtggtgg cagcagcgcc agtagcacgt 2161 gtagcaagag cagctttgac tacacacatg acatggaggc cgcacacatg gcagccacag 2221 ccattctcaa cctgtccaca cgttgtcgtg aaatgccaca gaacctgtcc accaagccac 2281 aggacctgtg tactgcccgg aacccagaca tggaggtgga tgagaatggc accctggacc 2341 tgagcatgaa caagcagagg cctcgagaca gctgctgccc agtcctgaca cccctggaac 2401 ccatgtctcc gcagcagcag gccgtgatga gcagccgatg cttccagctg agcgaggggg 2461 attgctggga cttgcctgta gactacacca aaatgaagcc tcggagggta gatgaggatg 2521 agcccaaaga gattacccca gaagacttgg acccattcca ggaggctctg gaagaaagac 2581 ggtatccagg ggaggtgacc atcccaagcc ccaaacccaa gtaccctcag tgcaaggaaa 2641 gcaaaaagga cttaataact ctgtctggct gccccctggc ggacaaaagc attcgaagta 2701 tgctggccac cagttcccaa gagctcaagt gccccacccc tggctgtgac ggttctggac 2761 acatcactgg caattacgct tctcatcgaa gcctttctgg gtgcccgaga gcaaagaaga 2821 gtggcatccg gatagcacag agcaaagagg acaaggaaga ccaggagcca atcaggtgtc 2881 cggtacctgg ctgtgacggt cagggacaca tcactgggaa gtatgcatcc caccgcagcg 2941 cctccgggtg tcccttggca gccaagaggc agaaagatgg gtaccttaat ggctcccagt 3001 tctcctggaa gtcggtcaag acggagggca tgtcctgccc tacccccggg tgtgatgggt 3061 caggacacgt cagtggcagc ttcctcacac accgcagctt gtcaggatgt ccaagagcca 3121 catcagcaat gaagaaagca aagctgtctg gagaacagat gttgactatc aagcagcgag 3181 ccagcaacgg tatagaaaat gatgaagaaa tcaagcagtt agatgaagag atcaaggagc 3241 ttaatgagtc caattcccag atggaggctg acatgatcaa actcagaact cagatcacca 3301 caatggagag caacctgaag acgattgagg aggagaacaa agtcattgaa cagcagaatg 3361 agtcgctctt gcacgagttg gccaacctga gccagtccct gatccacagc ctcgccaaca 3421 tccagctgcc tcacatggat ccaatcaatg aacaaaattt tgatgcttac gtgactactt 3481 tgacggaaat gtatacaaat caagatcgtt atcagagtcc agaaaataaa gccctactgg 3541 aaaatataaa gcaggctgtg agaggaattc aggtctgaca attcgatatc aagcttatcg 3601 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg 3661 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc 3721 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt 3781 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca 3841 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc 3901 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc 3961 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc 4021 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc 4081 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc 4141 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc 4201 gtcgacctcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca 4261 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac 4321 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa 4381 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc 4441 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag 4501 ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga 4561 aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg 4621 ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc 4681 atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct 4741 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc 4801 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc 4861 tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca 4921 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc 4981 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg 5041 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg 5101 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag 5161 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta 5221 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 5281 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 5341 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 5401 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 5461 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 5521 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc 5581 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg 5641 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 5701 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa 5761 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca 5821 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 5881 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag 5941 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg 6001 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata 6061 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc 6121 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt 6181 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt 6241 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga 6301 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc 6361 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg 6421 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc 6481 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat 6541 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc 6601 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 6661 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat 6721 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 6781 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 6841 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 6901 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 6961 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 7021 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 7081 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 7141 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 7201 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 7261 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 7321 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 7381 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 7441 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 7501 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 7561 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc 7621 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 7681 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 7741 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 7801 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 7861 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 7921 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 7981 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 8041 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 8101 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 8161 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 8221 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 8281 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 8341 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 8401 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 8461 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 8521 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 8581 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 8641 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 8701 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 8761 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 8821 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 8881 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc 8941 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc 9001 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg 9061 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt 9121 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa 9181 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg 9241 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg 9301 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta 9361 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt 9421 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac 9481 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt 9541 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac 9601 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt 9661 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat 9721 ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga 9781 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 9841 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 9901 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg 9961 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc 10021 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga 10081 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt 10141 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg 10201 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa 10261 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat 10321 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa 10381 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc 10441 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa 10501 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt 10561 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc 10621 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt 10681 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct 10741 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag 10801 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac 10861 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca 10921 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt 10981 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa 11041 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt 11101 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt 11161 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag 11221 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc 11281 cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat 11341 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag 11401 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc 11461 aaaattttcg ggtttattac agggacagca gagatccagt ttggttactc gagtttacca 11521 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga 11581 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt 11641 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt 11701 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag 11761 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagctc ggtacccggg 11821 tcgagtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag 11881 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc 11941 agcctccgcg gccccg //
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