| 质粒名称: | hRad51(K133R)–Cas9(D10A) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 125568 |
| 存储实验室: | David Liu |
|
载体骨架基本信息 |
|
| 载体名称: | CMV |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 3300 bp |
| 完整质粒大小: | 8712 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达,CRISPR |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Rad51(K133R)–Cas9(D10A),RDN(K133R) |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | hRad51(K133R)–Cas9(D10A) was a gift from David Liu (Addgene plasmid # 125568 ; http://n2t.net/addgene:125568 ; RRID:Addgene_125568) |
| 参考文献部分: | Development of hRad51-Cas9 nickase fusions that mediate HDR without double-stranded breaks. Rees HA, Yeh WH, Liu DR. Nat Commun. 2019 May 17;10(1):2212. doi: 10.1038/s41467-019-09983-4. 10.1038/s41467-019-09983-4 PubMed 31101808 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
–Cas9(D10A).png)
载体简介
载体序列
LOCUS hRad51(K133R)–Cas9(D10A) 8712 bp ds-DNA circular SYN 26-JUL-2019
DEFINITION Expresses the Rad51(K133R)–Cas9(D10A) fusion construct in mammalian
cells under CMV promoter.
ACCESSION .
VERSION .
KEYWORDS hRad51(K133R)–Cas9(D10A)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8712)
AUTHORS Rees HA, Yeh WH, Liu DR
TITLE Development of hRad51-Cas9 nickase fusions that mediate HDR without
double-stranded breaks.
JOURNAL Nat Commun. 2019 May 17;10(1):2212. doi: 10.1038/s41467-019-09983-4.
PUBMED 31101808
REFERENCE 2 (bases 1 to 8712)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 26, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8712
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 132..335
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 286..306
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 377..396
/label=T7
/note="T7 promoter, forward primer"
promoter 377..395
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 403..412
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 415..435
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 1570..5670
/codon_start=1
/product="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
/label=Cas9(D10A)
/note="generates RNA-guided single strand nicks in DNA due
to the D10A mutation in the RuvC catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 5671..5718
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
CDS 5727..5744
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(5767..5784)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5773..5997
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(6068..6084)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6068..6084)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6081..6103)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6092..6108
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6116..6146)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6161..6182
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6299..6316)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6470..7058)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6550..6569)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7229..8089)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7852..7871
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8090..8194)
/gene="bla"
/label=AmpR promoter
primer_bind complement(8273..8292)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 8464..131
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
ORIGIN
1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat ggccccaaag
421 aagaagcgga aggtcggtat ccacggagtc ccagcagccg cgatgcagat gcagttggaa
481 gcgaatgcag atactagtgt cgaggaagag tcatttggcc cgcaacccat ctcgcgttta
541 gagcaatgtg gcatcaatgc aaacgatgtg aaaaaattag aggaagctgg attccacacg
601 gtcgaagcgg tcgcatacgc accgaaaaaa gagctgatca acatcaaagg catcagcgag
661 gcgaaagccg ataagattct tgcagaggcg gcgaaattag ttcccatggg atttacgacg
721 gcgactgagt tccatcaacg tcgttccgag atcattcaaa tcacgaccgg aagcaaggag
781 ttggataaac tgcttcaagg aggcatcgaa actggctcga tcactgaaat gtttggagag
841 tttcgtactg gccgaaccca gatctgccac acattggccg tcacttgcca gcttccgatc
901 gatcgtggtg ggggcgaggg gaaggccatg tacattgata ctgaggggac gtttcgtccc
961 gaacgccttc ttgcggtggc agagcgttac ggcttgtcag ggtctgatgt tcttgacaat
1021 gttgcctatg ctcgcgcatt taataccgac caccagacgc agcttctgta ccaagcaagc
1081 gcgatgatgg tagagtctcg ttacgcttta ttgattgttg actcagcaac agcgttgtac
1141 cgcacagatt attctgggcg cggcgaattg tcggcacgcc aaatgcattt agcccgcttt
1201 ttgcgtatgt tgctgcgcct tgcagacgaa tttggggtgg ctgtggtgat caccaaccaa
1261 gttgtagcac aggtggacgg tgccgctatg tttgccgctg atccgaaaaa acccatcgga
1321 gggaatatta tcgcacatgc atcgacaact cgcttatatc tgcgtaaggg ccgtggagaa
1381 acacgcatct gcaaaatcta tgactcccca tgcctgccgg aggcggaagc catgttcgca
1441 attaacgcag atggcgtagg ggacgccaaa gattctggtg gttcttctgg tggttctagc
1501 ggcagcgaga ctcccgggac ctcagagtcc gccacacccg aaagttctgg tggttcttct
1561 ggtggttctg acaagaagta cagcatcggc ctggccatcg gcaccaactc tgtgggctgg
1621 gccgtgatca ccgacgagta caaggtgccc agcaagaaat tcaaggtgct gggcaacacc
1681 gaccggcaca gcatcaagaa gaacctgatc ggagccctgc tgttcgacag cggcgaaaca
1741 gccgaggcca cccggctgaa gagaaccgcc agaagaagat acaccagacg gaagaaccgg
1801 atctgctatc tgcaagagat cttcagcaac gagatggcca aggtggacga cagcttcttc
1861 cacagactgg aagagtcctt cctggtggaa gaggataaga agcacgagcg gcaccccatc
1921 ttcggcaaca tcgtggacga ggtggcctac cacgagaagt accccaccat ctaccacctg
1981 agaaagaaac tggtggacag caccgacaag gccgacctgc ggctgatcta tctggccctg
2041 gcccacatga tcaagttccg gggccacttc ctgatcgagg gcgacctgaa ccccgacaac
2101 agcgacgtgg acaagctgtt catccagctg gtgcagacct acaaccagct gttcgaggaa
2161 aaccccatca acgccagcgg cgtggacgcc aaggccatcc tgtctgccag actgagcaag
2221 agcagacggc tggaaaatct gatcgcccag ctgcccggcg agaagaagaa tggcctgttc
2281 ggaaacctga ttgccctgag cctgggcctg acccccaact tcaagagcaa cttcgacctg
2341 gccgaggatg ccaaactgca gctgagcaag gacacctacg acgacgacct ggacaacctg
2401 ctggcccaga tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac
2461 gccatcctgc tgagcgacat cctgagagtg aacaccgaga tcaccaaggc ccccctgagc
2521 gcctctatga tcaagagata cgacgagcac caccaggacc tgaccctgct gaaagctctc
2581 gtgcggcagc agctgcctga gaagtacaaa gagattttct tcgaccagag caagaacggc
2641 tacgccggct acattgacgg cggagccagc caggaagagt tctacaagtt catcaagccc
2701 atcctggaaa agatggacgg caccgaggaa ctgctcgtga agctgaacag agaggacctg
2761 ctgcggaagc agcggacctt cgacaacggc agcatccccc accagatcca cctgggagag
2821 ctgcacgcca ttctgcggcg gcaggaagat ttttacccat tcctgaagga caaccgggaa
2881 aagatcgaga agatcctgac cttccgcatc ccctactacg tgggccctct ggccagggga
2941 aacagcagat tcgcctggat gaccagaaag agcgaggaaa ccatcacccc ctggaacttc
3001 gaggaagtgg tggacaaggg cgcttccgcc cagagcttca tcgagcggat gaccaacttc
3061 gataagaacc tgcccaacga gaaggtgctg cccaagcaca gcctgctgta cgagtacttc
3121 accgtgtata acgagctgac caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc
3181 ttcctgagcg gcgagcagaa aaaggccatc gtggacctgc tgttcaagac caaccggaaa
3241 gtgaccgtga agcagctgaa agaggactac ttcaagaaaa tcgagtgctt cgactccgtg
3301 gaaatctccg gcgtggaaga tcggttcaac gcctccctgg gcacatacca cgatctgctg
3361 aaaattatca aggacaagga cttcctggac aatgaggaaa acgaggacat tctggaagat
3421 atcgtgctga ccctgacact gtttgaggac agagagatga tcgaggaacg gctgaaaacc
3481 tatgcccacc tgttcgacga caaagtgatg aagcagctga agcggcggag atacaccggc
3541 tggggcaggc tgagccggaa gctgatcaac ggcatccggg acaagcagtc cggcaagaca
3601 atcctggatt tcctgaagtc cgacggcttc gccaacagaa acttcatgca gctgatccac
3661 gacgacagcc tgacctttaa agaggacatc cagaaagccc aggtgtccgg ccagggcgat
3721 agcctgcacg agcacattgc caatctggcc ggcagccccg ccattaagaa gggcatcctg
3781 cagacagtga aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa gcccgagaac
3841 atcgtgatcg aaatggccag agagaaccag accacccaga agggacagaa gaacagccgc
3901 gagagaatga agcggatcga agagggcatc aaagagctgg gcagccagat cctgaaagaa
3961 caccccgtgg aaaacaccca gctgcagaac gagaagctgt acctgtacta cctgcagaat
4021 gggcgggata tgtacgtgga ccaggaactg gacatcaacc ggctgtccga ctacgatgtg
4081 gaccatatcg tgcctcagag ctttctgaag gacgactcca tcgacaacaa ggtgctgacc
4141 agaagcgaca agaaccgggg caagagcgac aacgtgccct ccgaagaggt cgtgaagaag
4201 atgaagaact actggcggca gctgctgaac gccaagctga ttacccagag aaagttcgac
4261 aatctgacca aggccgagag aggcggcctg agcgaactgg ataaggccgg cttcatcaag
4321 agacagctgg tggaaacccg gcagatcaca aagcacgtgg cacagatcct ggactcccgg
4381 atgaacacta agtacgacga gaatgacaag ctgatccggg aagtgaaagt gatcaccctg
4441 aagtccaagc tggtgtccga tttccggaag gatttccagt tttacaaagt gcgcgagatc
4501 aacaactacc accacgccca cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc
4561 aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg actacaaggt gtacgacgtg
4621 cggaagatga tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc
4681 tacagcaaca tcatgaactt tttcaagacc gagattaccc tggccaacgg cgagatccgg
4741 aagcggcctc tgatcgagac aaacggcgaa accggggaga tcgtgtggga taagggccgg
4801 gattttgcca ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt gaaaaagacc
4861 gaggtgcaga caggcggctt cagcaaagag tctatcctgc ccaagaggaa cagcgataag
4921 ctgatcgcca gaaagaagga ctgggaccct aagaagtacg gcggcttcga cagccccacc
4981 gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag
5041 agtgtgaaag agctgctggg gatcaccatc atggaaagaa gcagcttcga gaagaatccc
5101 atcgactttc tggaagccaa gggctacaaa gaagtgaaaa aggacctgat catcaagctg
5161 cctaagtact ccctgttcga gctggaaaac ggccggaaga gaatgctggc ctctgccggc
5221 gaactgcaga agggaaacga actggccctg ccctccaaat atgtgaactt cctgtacctg
5281 gccagccact atgagaagct gaagggctcc cccgaggata atgagcagaa acagctgttt
5341 gtggaacagc acaagcacta cctggacgag atcatcgagc agatcagcga gttctccaag
5401 agagtgatcc tggccgacgc taatctggac aaagtgctgt ccgcctacaa caagcaccgg
5461 gataagccca tcagagagca ggccgagaat atcatccacc tgtttaccct gaccaatctg
5521 ggagcccctg ccgccttcaa gtactttgac accaccatcg accggaagag gtacaccagc
5581 accaaagagg tgctggacgc caccctgatc caccagagca tcaccggcct gtacgagaca
5641 cggatcgacc tgtctcagct gggaggcgac aaaaggccgg cggccacgaa aaaggccggc
5701 caggcaaaaa agaaaaagtg accggtcatc atcaccatca ccattgagtt taaacccgct
5761 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
5821 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
5881 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
5941 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
6001 ctgaggcgga aagaaccagc tggggctcaa taccgtcgac ctctagctag agcttggcgt
6061 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
6121 tacgagccgg aagcataaag tgtaaagcct agggtgccta atgagtgagc taactcacat
6181 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
6241 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
6301 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
6361 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
6421 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
6481 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
6541 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
6601 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
6661 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
6721 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
6781 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
6841 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
6901 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
6961 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
7021 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
7081 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
7141 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
7201 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
7261 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
7321 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
7381 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
7441 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
7501 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
7561 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
7621 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
7681 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
7741 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
7801 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
7861 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
7921 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
7981 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
8041 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
8101 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
8161 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
8221 acgtcgacgg atcgggagat cgatctcccg atcccctagg gtcgactctc agtacaatct
8281 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
8341 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
8401 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
8461 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
8521 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
8581 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
8641 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
8701 atcaagtgta tc
//
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