LOCUS mPiezo2-CMV-Sport6 12826 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION mouse Piezo2.
ACCESSION .
VERSION .
KEYWORDS mPiezo2-CMV-Sport6
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12826)
AUTHORS Coste B, Murthy SE, Mathur J, Schmidt M, Mechioukhi Y, Delmas P,
Patapoutian A
TITLE Piezo1 ion channel pore properties are dictated by C-terminal
region.
JOURNAL Nat Commun. 2015 May 26;6:7223. doi: 10.1038/ncomms8223.
PUBMED 26008989
REFERENCE 2 (bases 1 to 12826)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12826
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 1..589
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 490..509
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 743..760
/label=L4440
/note="L4440 vector, forward primer"
enhancer 1048..1351
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1352..1555
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 1506..1526
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 1552..1576
/label=LNCX
/note="Human CMV promoter, forward primer"
primer_bind 1681..1697
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 1681..1697
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
promoter 1708..1726
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 1708..1725
/label=SP6
/note="SP6 promoter, forward primer"
protein_bind 1727..1751
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
regulatory 1758..1767
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
protein_bind complement(10277..10301)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
primer_bind complement(10302..10321)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(10303..10321)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(10332..10349)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(10332..10348)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(10341..10363)
/label=M13/pUC Forward
/note="In lacZ gene"
polyA_signal 10753..10887
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(10790..10809)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 10844..10863
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 11013..11467
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(11100..11119)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 11309..11330
/label=F1ori-F
/note="F1 origin, forward primer"
CDS 11754..12614
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(11972..11991)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
protein_bind complement(12716..12749)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
ORIGIN
1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagagctt gcaattcgcg
841 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
901 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
961 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtattacg
1021 aggccctttc acgcattaga tgcatgtcgt tacataactt acggtaaatg gcccgcctgg
1081 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
1141 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
1201 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
1261 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
1321 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
1381 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
1441 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
1501 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt
1561 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
1621 ccgggaccga tccagcctcc ggactctagc ctaggccgcg ggacggataa caatttcaca
1681 caggaaacag ctatgaccat taggcctatt taggtgacac tatagaacaa gtttgtacaa
1741 aaaagcaggc tggtaccgcc accatggctt cggaagtggt gtgcgggctg atcttcaggc
1801 tgctgcttcc catctgcctg gcagtagcat gtgcgttccg gtacaatggg ctctcctttg
1861 tctaccttat ctacctcttg ctcattcctc tgttctcaga accaaccaaa gcaacgatgc
1921 aaggacacac agggcggttg ctacagtccc tgtgcatcac cagcctctca tttctgctgc
1981 ttcacatcat tttccacatc acactggcca gtctggaagc tcaacaccgc atcacacctg
2041 cttacaactg ctcaacatgg gaaaagacct tccggcagat tggctttgaa agcttgaagg
2101 gagctgatgc cggcaatggc atcagggtat ttgtgcctga tatcgggatg ttcattgcaa
2161 gtctgaccat ctggctggtc tgcagaacca ttgttaagaa accagacaca gaagaaatag
2221 cccaattgaa ttctgagtgt gaaaatgaag aattggctgg aggagaaaag atggattcag
2281 aggaggcgct gatttatgaa gaggacttag atggagaaga aggcatggaa ggagagctag
2341 aagaaagcac aaaactaaaa atacttcgca ggttcgcctc tgtcgcctcc aaactgaagg
2401 agttcattgg caatatgatc accacggcgg gcaaggtcgt ggtgaccatc ctactgggct
2461 cctcgggcat gatgctgcca tctttgacct cggctgtgta cttctttgta tttctgggcc
2521 tgtgtacctg gtggtcctgg tgccgaactt tcgacccatt gctgttcggc tgcctctgtg
2581 tcctgctggc aatcttcact gctgggcacc tgattggact ttatctgtac cagttccaat
2641 tcttccaaga agcggtcccg cccaatgact actatgcaag gttgtttggg atcaagtccg
2701 ttatccagac agactgtgcc agcacctgga agatcatagt aaacccagac ctgtcctggt
2761 atcaccatgc caaccccatc ctcctgctgg tgatgtacta tacgctggcc acgctgattc
2821 gcatctggct acaagagcct cttgtgcaag aggagatggc gaaggaagat gagggtgccc
2881 tggattgcag ctccaatcag aacacggcag agaggaggcg gagcctgtgg tatgcaaccc
2941 agtacccaac agatgagaga aaacttttat ccatgaccca agatgactac aaaccatctg
3001 atggcctgct ggtgaccgtg aatggcaacc ctgtggacta ccacaccatc cacccaagcc
3061 tgcccataga gaatggccct gccaagacgg acctgtacac cactccccag taccggtggg
3121 agccttcgga ggagtcctca gaaaagaaag aggaagaaga ggacaagaga gaagactccg
3181 aaggggaagg gagccaggag gagaaaagga gtgtgagaat gcacgccatg gtcgccgtgt
3241 tccagttcat catgaagcag agttacatct gtgccctcat cgccatgatg gcatggagca
3301 tcacctacca cagctggctg actttcgtgc tgttgatctg gtcctgcacc ctctggatga
3361 ttcgcaacag gaggaagtat gccatgatca gttctccttt catggtcgtc tatgcgaatc
3421 tgctgctggt gctgcagtac atatggagct tcgagctccc cgagattaag aaggtccccg
3481 ggtttctaga aaagaaagag ccgggagaac ttgcttcaaa gattctcttc accatcacgt
3541 tctggctgct cctgaggcag catctcacag agcagaaggc cctgagggaa aaggaagcac
3601 ttctttctga agtcaaaatt ggaagtcaag aacttgaaga aaaggaagat gaggagctcc
3661 aagatgtaca agtggaaggg gagcccacag agaaggaaga agaagaggaa gaggaaataa
3721 aggaagaaag gcatgaggta aagaaggaag aggaagagga agtggaggaa gatgatgacc
3781 aggacatcat gaaggtgctg ggtaatctgg tggtggccct cttcatcaag tattggatct
3841 acgtctgcgg gggcatgttc ttttttgtca gctttgaggg gaaaatcgtc atgtacaaaa
3901 tcatctacat ggtgctgttc ctgttctgcg tggccttgta ccaggtgcac tacgagtggt
3961 ggaggaagat cctgaagtac ttctggatgt cggtggtcat ttataccatg ttggtgctca
4021 tcttcatata cacttaccag tttgaaaact tcccaggcct gtggcaaaac atgaccggat
4081 taaagaaaga aaagctcgag gacctcggcc tgaagcagtt cacggtcgct gaactgttca
4141 ctcgcatatt catccccacc tcctttctac tcgtctgcat cctacacctc cactacttcc
4201 acgatcggtt cctggaactt acagacctca agtccatccc cagcaaagaa gacaacacca
4261 tctacagcca tgccaaagtc aatggtcgcg tgtacctaat aataaatagg ttggcccacc
4321 ctgaaggaag tcttccagac ttagccatca tgaatatgac ggccagcctt gataagcctg
4381 aggtgcagaa gttggcagag tctggggagg agaggccaga agagtgtgtc aagaagacgg
4441 agaagggtga ggctgggaaa gacagtgatg agtctgagga ggaggaagat gaagaagagg
4501 aatccgagga agaagaaagc tcagacctca ggaacaagtg gcacctggtg attgaccggc
4561 tcactgtgct ctttctaaaa ttcctggagt atttccacaa gttgcaggtg tttatgtggt
4621 ggatcttgga gctgcacatc atcaaaatcg tctcatcgta cattatctgg gtgactgtga
4681 aagaggtgtc tctgttcaac tatgtatttt tgatttcttg ggcatttgct ctgccatatg
4741 ccaaactccg tcgtgcggct tccagtgtct gcactgtctg gacatgtgtg atcatcgtct
4801 gcaaaatgtt gtaccagctg caaaccatta aacctgagaa cttttctgtc aactgctcct
4861 tgcccaatga aaatcaaacc aacattcccc ttcatgagtt gaacaagtct cttctctaca
4921 gtgcccctgt tgatcccact gagtgggtag gcctgaggaa gtcctctcct ttgctcgtct
4981 acctgaggaa caacctcctg atgctggcga ttttggcctt tgaagtcacc gtttaccgcc
5041 atcaggagta ctatcgaggc cgaaacaacc tgactgctcc agtgtctaaa actatctttc
5101 atgacatcac aaggttacat ctagatgatg ggcttatcaa ttgtgccaaa tattttgtaa
5161 attacttctt ctacaagttt ggcctagaga cctgtttcct aatgtccgtg aatgtaattg
5221 gacagcgaat ggacttctac gccatgattc atgcctgttg gttgatcggt gtcttgtatc
5281 gacgcagaag aaaagccatt gccgaggtct ggcccaagta ctgctgcttc ctggcttgca
5341 tcatcacctt ccagtacttc gtctgcatcg gcatcccccc tgccccttgc cgagattatc
5401 catggagatt taagggagcc tacttcaatg ataacattat caagtggctg tacttcccgg
5461 atttcattgt gcggcccaac cccgtgtttc ttgtctatga cttcatgctg ctcctgtgtg
5521 cttccttaca acgacagatc tttgaggacg aaaacaaggc agccgtgcgc atcatggccg
5581 gggacaatgt cgagatctgc atgaaccttg atgcagcctc gttcagccag cataaccctg
5641 tgccagattt cattcactgc agatcttact tggacatgtc caaagtgatc atcttcagct
5701 acctcttctg gttcgtcctc accatcatct tcattacggg aacaaccagg atcagcatat
5761 tttgcatggg ctacttggtg gcctgtttct atttcctgct ctttggaggg gatctgctgc
5821 tgaagcccat caagagcatc cttcgttact gggactggct gattgcatac aacgtgtttg
5881 tgatcaccat gaagaatata ctgtcaatag gagcctgtgg atacattggc gcactagtaa
5941 gaaacagctg ctggttgatc caagcgttca gcctggcctg caccgtgaaa ggctatcaga
6001 tgcctgaaga tgactccaga tgcaagctgc ccagtgggga ggcagggatc atctgggaca
6061 gcatctgctt tgcgtttctg ctgctgcaga ggagagtctt catgagctac tacttcctgc
6121 atgtcgtggc tgacattaaa gcatcccaga tcctggcatc cagaggggcc gaacttttcc
6181 aggctacaat tgtgaaagct gtaaaggcaa gaattgaaga agagaaaaaa tccatggacc
6241 agctgaagag acagatggat cgcatcaaag cccggcaaca aaagtacaaa aagggtaagg
6301 agaggatgct gagcttgacc caggagtcag gggaaggcca ggacatccag aaagtctcag
6361 aagaagatga tgaaagagaa gcagacaaac agaaagccaa gggcaaaaaa aagcagtggt
6421 ggcggccttg ggttgatcat gcttccatgg tcaggagtgg agattattat ttgtttgaaa
6481 cggatagtga agaggaggaa gaagaggaac ttaaaaagga ggatgaagaa cccccacgga
6541 agtcagcatt ccagttcgtc taccaagcct ggattactga tcctaaaacg gcactccgac
6601 agaggaggaa agagaagaaa aagttggcca gagaagagca gaaggagaga cgcaaaggat
6661 ctggggacgg tccagtggaa tgggaagatc gagaggatga gccagtcaaa aagaaatctg
6721 atggaccaga taatatcatc aaaaggatat ttaatatcct gaagttcaca tgggttctgt
6781 tcctggccac agtggatagt ttcaccactt ggctcaactc catctcaagg gaacacatcg
6841 acatatccac agtcctgcgg attgaaagat gcatgctgac tcgagagata aaaaagggca
6901 atgttccaac acgggaaagc atccacatgt actaccagaa ccacattatg aacctttccc
6961 gagaatccgg cctggacaca atcgacgagc actcgggggc tggctccagg gcccaggcag
7021 cacataggat ggatagctta gattcccggg acagtatctc cagctgctac actgaagcga
7081 ccctgctgat ctcaaggcag tccacccttg atgatttaga tggacaagac cccgttccta
7141 aaacaagcga gcgtgctcgg cctaggctcc gtaaaatgtt cagcctggac atgtcctctt
7201 cgtcagctga cagtggcagt gtagcatcaa gcgagcccac acagtgtacc atgctgtatt
7261 cgcgccaagg aactacggaa accatcgagg aggtggaggc tgaagctgag gaggaggtgg
7321 tagaaggtct ggagcctgag ctccatgatg cagaagaaaa ggaatacgca gctgaatatg
7381 aagcaggggt agaggagatc agcctcactc cagatgagga attgcctcag ttctctacag
7441 atgactgtga ggcgccgccc tcttacagca aggctgtcag ctttgagcat ctatcgtttg
7501 cgtcgcaaga tgactctggg gccaagaacc acatggtggt cagtcctgat gacagccgta
7561 cagacaagct ggagtcaagc atcttaccac ctctgactca tgagctgaca gccagcgatc
7621 tgctgatgag caagatgttc catgatgatg agcttgagga gtctgagaag ttctacgtgg
7681 accagccccg gtttttgctg ctattctatg ccatgtataa caccctggtg gcccgctcag
7741 aaatggtgtg ctatttcgtg attatcctga accacatgac ctctgcctcc atcatcactc
7801 tcctgttgcc catcttgatc ttcctctggg ccatgctgtc tgttcccagg cccagccgac
7861 gcttctggat gatggccatc gtctacactg aggtggcgat cgtagtgaaa tacttcttcc
7921 agttcggctt ctttccctgg aataaggact tggaaatata caaggaaagg ccctactttc
7981 caccgaacat catcggggtg gagaagaagg agggctatgt tctttatgac ctcattcagc
8041 tgctggctct attcttccat cgctccattc tgaagtgtca tggtctatgg gacgaggatg
8101 acattgttga cagtaacaca gacaaggaag gatcggatga cgagctgtcc ctcgatcagg
8161 gcagaagagg ctcctcagac tcactgaagt ctatcaacct ggctgcctct gtagagtctg
8221 tccacgtgac cttccccgag cagccagcgg ccatcaggag aaaacgctcc tgcagtagct
8281 ctcagatctc cccacggtcc agcttctctt caaataggtc caaaagaggc agcacaagca
8341 cccggaacag tagccagaaa ggaagcagtg ttttaagcct taaacagaaa agcaaacgag
8401 aactttacat ggaaaagctt caagagcatt taatcaaagc aaaggcgttt accataaaga
8461 agactctaca gatctatgtc cccattcggc aattcttcta tgacctcatc cacccagact
8521 acagtgctgt gactgatgta tacgtgctga tgtttctggc tgacactgta gacttcatta
8581 tcatcgtctt tggattctgg gcctttggga aacactcggc agctgcagat atcacctctt
8641 ctctatcaga agaccaggtc cccgggccat tcctggtgat ggtcctcatt cagtttggaa
8701 ccatggtggt ggaccgtgca ctctacctca ggaagactgt gctgggcaag gtcatcttcc
8761 aggtcatcct cgtgtttggg attcacttct ggatgttctt catcttaccc ggtgtgactg
8821 agaggaaatt cagccagaac ctggtggcac agctttggta ctttgtgaag tgtgtgtact
8881 ttgggctgtc tgcctatcaa atccgctgtg gctacccaac ccgtgtgctc gggaacttcc
8941 tcacaaagag ctacaattac gtcaacctct tcttgtttca agggttccgc ctggtcccat
9001 ttctgactga gctgcgggct gtgatggatt gggtgtggac agatacaacc ctgagcctct
9061 ccagctggat ttgtgtggag gacatttacg cgcacatttt tatcttaaag tgctggcgag
9121 agtcggaaaa gagataccct caacccaggg gtcagaagaa gaagaaagcc gtgaagtacg
9181 gcatgggcgg catgatcatc gtccttctca tctgtatcgt ctggtttcct ctgctcttca
9241 tgtctttgat caaatctgtc gcaggggtta tcaaccaacc cctggatgtg tccgtcacaa
9301 ttaccctggg aggttatcag cctatcttca caatgagcgc ccagcaaagc cagctgaaag
9361 ttatggataa ttcaaagtat aatgaatttc taaaatcttt cggccccaac agtggcgcaa
9421 tgcaatttct ggagaactat gaaagggaag acgtaacagt agcagaactg gaagggaact
9481 cgaattcttt gtggaccatc agccctccca gtaagcagaa aatgatacag gagctcaccg
9541 accccaatag ttgcttctct gttgtttttt catggagtat ccagagaaac atgactttgg
9601 gtgcaaaagc cgaaatagca acggataagc tttctttccc tcttgcggtc gcaacacgga
9661 atagcatagc taaaatgatt gctggcaatg acacagaaag ttcaaacacg ccagtgacaa
9721 tagagaagat ctacccatac tatgtgaagg cacccagcga ttcaaactca aagcctataa
9781 aacagcttct gtctgaaaat aatttcatga atatcaccat cattttgttc agagacaatg
9841 tcacaaagtc caatagtgag tggtgggttc tcaacctgac cggaagtagg atattcaacc
9901 aggggtccca agccttggaa ctggtggtct tcaatgacaa agtcagccct ccaagtctag
9961 gcttcttggc tggctatggt atcatgggat tgtatgcatc tgttgtcctt gtaattggga
10021 aatttgttcg tgagttcttc agtgggatct ctcattccat catgtttgaa gagcttccaa
10081 atgtggacag aatcttgaag ttgtgcacag atatattcct cgtgagggag acaggggagt
10141 tggaactgga ggaagacctc tacgccaagt tgatattcct gtacaggtca ccagaaacca
10201 tgatcaagtg gactagagaa aaaacaaact gagcggccgc tctagagtat ccctcgaggg
10261 gcccaagctt acgcgtaccc agctttcttg tacaaagtgg tccctatagt gagtcgtatt
10321 ataagctagg cactggccgt cgttttacaa cgtcgtgact gggaaaactg ctagcttggg
10381 atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag
10441 atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actagctgca
10501 tatgcttgct gcttgagagt tttgcttact gagtatgatt tatgaaaata ttatacacag
10561 gagctagtga ttctaattgt ttgtgtattt tagattcaca gtcccaaggc tcatttcagg
10621 cccctcagtc ctcacagtct gttcatgatc ataatcagcc ataccacatt tgtagaggtt
10681 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
10741 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
10801 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
10861 atcaatgtat cttatcatgt ctggatcgat cctgcattaa tgaatcggcc aacgcgcggg
10921 gagaggcggt ttgcgtattg gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
10981 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
11041 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
11101 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
11161 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
11221 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
11281 tttgacgttg gagtccacgt tcttaatagt ggactcttgt tccaaactgg aacaacactc
11341 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
11401 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
11461 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
11521 aaatacattc aaatatgtat ccgctcatgc caggtcttgg actggtgaga acggcttgct
11581 cggcagcttc gatgtgtgct ggagggagaa taaaggtcta agatgtgcga tagagggaag
11641 tcgcattgaa ttatgtgctg tgtagggatc gctggtatca aatatgtgtg cccacccctg
11701 gcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
11761 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
11821 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
11881 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
11941 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
12001 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
12061 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
12121 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
12181 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
12241 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
12301 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
12361 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
12421 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
12481 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
12541 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
12601 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
12661 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgccataac
12721 ttcgtataat gtatgctata cgaagttatg gcatgaccaa aatcccttaa cgtgagtttt
12781 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttc
//
