| 质粒名称: | mVenus-UbiquitinC-N-18 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 56339 |
| 存储实验室: | Michael Davidson |
|
载体骨架基本信息 |
|
| 载体名称: | mVenus-N1 |
| 空载体大小: | 4750 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | UbiquitinC |
| 物种来源: | H. sapiens (human) |
| 基因突变: | aa 2-76 of NP_003324.1 |
| 启动子: | CMV |
| 标签/融合蛋白: | mVenus (C terminal on backbone) |
| 克隆方法: | 未知 |
| 5’测序引物 | CMV-F |
| 3’测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | mVenus-UbiquitinC-N-18 was a gift from Michael Davidson (Addgene plasmid # 56339 ; http://n2t.net/addgene:56339 ; RRID:Addgene_56339) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS mVenus-UbiquitinC-N-18 4939 bp ds-DNA circular SYN 03-FEB-2023
DEFINITION Localization: Membrane , Excitation: 515, Emission: 528.
ACCESSION .
VERSION .
KEYWORDS mVenus-UbiquitinC-N-18
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4939)
TITLE Davidson Green fluorescent proteins
REFERENCE 2 (bases 1 to 4939)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Feb 3, 2023 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4939
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 519..539
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
CDS 603..830
/codon_start=1
/product="human ubiquitin"
/label=ubiquitin
/translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
regulatory 879..888
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 885..1604
/codon_start=1
/product="Venus YFP with monomerizing A206K mutation (Nagai
et al., 2002; Kremers et al., 2006)"
/label=mVenus
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(930..951)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1191..1210)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1538..1559
/label=EGFP-C
/note="EGFP, forward primer"
polyA_signal 1727..1848
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1764..1783)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1818..1837
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(1855..2310)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1992..2013)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2204..2223
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2337..2441
/gene="bla"
/label=AmpR promoter
promoter 2443..2800
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(2466..2486)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2651..2786
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2713..2732
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 2835..3629
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2889..2908)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3499..3518
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(3817..3836)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 3861..3908
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4237..4825
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4726..4745
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca
601 ccatgcagat cttcgtgaag actctgactg gtaagaccat caccctcgag gttgagccca
661 gtgacaccat tgagaatgtc aaggcaaaga tccaagataa ggaaggcatc cctcctgacc
721 agcagaggct gatctttgct ggaaaacagc tggaagatgg gcgcaccctg tctgactaca
781 acatccagaa agagtccacc ctgcacctgg tactccgtct cagaggtggg ggtggaattc
841 tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg agcaagggcg
901 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
961 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
1021 agctgatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctgg
1081 gctacggcct gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca
1141 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
1201 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
1261 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
1321 acaacagcca caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact
1381 tcaagatccg ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga
1441 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt
1501 ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
1561 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaagcggc cgcgactcta
1621 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
1681 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
1741 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
1801 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg
1861 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
1921 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
1981 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
2041 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
2101 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
2161 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
2221 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
2281 ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg
2341 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
2401 aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca
2461 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
2521 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
2581 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
2641 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
2701 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
2761 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat
2821 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
2881 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
2941 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
3001 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
3061 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
3121 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
3181 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
3241 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
3301 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
3361 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
3421 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
3481 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
3541 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
3601 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
3661 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
3721 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
3781 gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc
3841 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc
3901 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag
3961 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc
4021 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg
4081 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
4141 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
4201 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
4261 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
4321 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
4381 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
4441 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
4501 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
4561 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
4621 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
4681 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
4741 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
4801 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
4861 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
4921 ccgtattacc gccatgcat
//
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