| 质粒名称: | pAAV-TRE-ChR2-YFP |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 110339 |
| 存储实验室: | Hyungbae Kwon |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS |
| 载体出品公司: | Stratagene |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达,AAV |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | tetO-ChR2-YFP |
| 物种来源: | 合成的 |
| 插入大小: | -- |
| 基因突变: | ChR2 H134R |
| GenBank编号 | -- |
| 启动子 | tetO |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BamHI(未破坏) |
| 3' 克隆位点: | HindIII(未破坏) |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pAAV-TRE-ChR2-YFP was a gift from Hyungbae Kwon (Addgene plasmid # 110339 ; http://n2t.net/addgene:110339 ; RRID:Addgene_110339) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pAAV-TRE-ChR2-YFP 6034 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION ChR2-YFP reporter vector of iTango and Cal-Light system for AAV
production.
ACCESSION .
VERSION .
KEYWORDS pAAV-TRE-ChR2-YFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6034)
TITLE fluorescent protein reporter vectors of iTango and Cal-Light system
for AAV production
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 6034)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6034
/organism="synthetic DNA construct"
/mol_type="other DNA"
repeat_region 1..141
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 12..141
/label=AAV2 ITR
protein_bind 298..568
/label=tetracycline response element
/bound_moiety="tetracycline repressor TetR"
/note="contains seven copies of the tetracycline operator
tetO"
promoter 601..639
/label=minimal CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 1699..2412
/codon_start=1
/product="enhanced YFP"
/label=EYFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
primer_bind complement(1741..1762)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(2002..2021)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 2349..2370
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 2449..3037
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(2502..2522)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(2920..2931)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(3072..3089)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 3078..3285
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
repeat_region 3308..3437
/label=AAV2 ITR (alternate)
/note="Functional equivalent of wild-type AAV2 ITR"
rep_origin complement(3499..4087)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3579..3598)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4258..5118)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4881..4900
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5119..5223)
/gene="bla"
/label=AmpR promoter
primer_bind 5291..5309
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(5347..5369)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 5469..5488
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
rep_origin complement(5505..5960)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5642..5663)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 5854..5873
/label=F1ori-R
/note="F1 origin, reverse primer"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
121 actccatcac taggggttcc tgcggccgca cgcgaaacaa ttctgcagga attcggggcc
181 gcggaggctg gatcggtccc ggtgtcttct atggaggtca aaacagcgtg gatggcgtct
241 ccaggcgatc tgacggttca ctaaacgagc tctgcttata taggtcgagt ttaccactcc
301 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
361 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
421 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
481 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
541 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagctcggta cccgggtcga
601 ggtaggcgtg tacggtggga ggcctatata agcagagctc gtttagtgaa ccgtcagatc
661 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
721 ctccgcggcc ccgaattcga gctcggtacc cggggatcca tggactatgg cggcgctttg
781 tctgccgtcg gacgcgaact tttgttcgtt actaatcctg tggtggtgaa cgggtccgtc
841 ctggtccctg aggatcaatg ttactgtgcc ggatggattg aatctcgcgg cacgaacggc
901 gctcagaccg cgtcaaatgt cctgcagtgg cttgcagcag gattcagcat tttgctgctg
961 atgttctatg cctaccaaac ctggaaatct acatgcggct gggaggagat ctatgtgtgc
1021 gccattgaaa tggttaaggt gattctcgag ttcttttttg agtttaagaa tccctctatg
1081 ctctaccttg ccacaggaca ccgggtgcag tggctgcgct atgcagagtg gctgctcact
1141 tgtcctgtca tccttatccg cctgagcaac ctcaccggcc tgagcaacga ctacagcagg
1201 agaaccatgg gactccttgt ctcagacatc gggactatcg tgtggggggc taccagcgcc
1261 atggcaaccg gctatgttaa agtcatcttc ttttgtcttg gattgtgcta tggcgcgaac
1321 acattttttc acgccgccaa agcatatatc gagggttatc atactgtgcc aaagggtcgg
1381 tgccgccagg tcgtgaccgg catggcatgg ctgtttttcg tgagctgggg tatgttccca
1441 attctcttca ttttggggcc cgaaggtttt ggcgtcctga gcgtctatgg ctccaccgta
1501 ggtcacacga ttattgatct gatgagtaaa aattgttggg ggttgttggg acactacctg
1561 cgcgtcctga tccacgagca catattgatt cacggagata tccgcaaaac caccaaactg
1621 aacatcggcg gaacggagat cgaggtcgag actctcgtcg aagacgaagc cgaggccgga
1681 gccgtgccag cggccgccgt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
1741 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
1801 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
1861 gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcagtgctt cgcccgctac
1921 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
1981 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
2041 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
2101 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
2161 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
2221 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
2281 ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag
2341 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
2401 gagctgtaca agttctgcta cgagaacgag gtgtaaaagc ttatcgataa tcaacctctg
2461 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
2521 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
2581 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
2641 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
2701 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
2761 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
2821 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctatgttgcc
2881 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
2941 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
3001 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgagc gctgctcgag
3061 agatcgatct gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
3121 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
3181 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
3241 cagcaagggg gaggattggg aagacaatag caggcatgct ggggacacgt gcggaccgag
3301 cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
3361 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggcg gcctcagtga gcgagcgagc
3421 gcgcagctgc ctgcaggaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
3481 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
3541 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
3601 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
3661 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
3721 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
3781 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
3841 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
3901 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
3961 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
4021 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
4081 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
4141 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
4201 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
4261 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
4321 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
4381 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
4441 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
4501 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
4561 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
4621 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
4681 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
4741 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
4801 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
4861 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
4921 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
4981 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
5041 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
5101 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
5161 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
5221 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
5281 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
5341 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
5401 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
5461 taactatgcg gcatcagagc agattgtact gagagtgcac cataaaattg taaacgttaa
5521 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaatagac
5581 cgaaatcggc aaaatccctt ataaatcaaa agaatagccc gagatagagt tgagtgttgt
5641 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
5701 aaccgtctat cagggcgatg gcccactacg tgaaccatca cccaaatcaa gttttttggg
5761 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
5821 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
5881 taaggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
5941 tgcgccgcta cagggcgcgt actatggttg ctttgacgta tgcggtgtga aataccgcac
6001 agatgcgtaa ggagaaaata ccgcatcagg cgcc
//
