LOCUS pBABEpuroUThTERT+U3-hTR-500 10323 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pBABEpuroUThTERT+U3-hTR-500
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10323)
AUTHORS Wong JM, Collins K
TITLE Telomerase RNA level limits telomere maintenance in X-linked
dyskeratosis congenita.
JOURNAL Genes Dev. 2006 Oct 15. 20(20):2848-58.
PUBMED 17015423
REFERENCE 2 (bases 1 to 10323)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10323
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..470
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 535..734
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 935..1351
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1281..1303
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1319..1335
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
CDS 1431..4829
/codon_start=1
/gene="TERT"
/product="human telomerase reverse transcriptase, the
catalytic subunit of telomerase"
/label=hTERT
/note="prevents telomere shortening and allows
postsenescent cells to proliferate beyond crisis (Counter
et al., 1998); also known as EST2"
/translation="MPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDPA
AFRALVAQCLVCVPWDARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFGFALL
DGARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFVLVA
PSCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPGAR
RRGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEE
ATSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYSSGDKEQ
LRPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGN
HAQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSP
WQVYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRGC
AWLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTETTFQKNRLFFY
RKSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVN
MDYVVGARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLGLDDIHRAWRTFV
LRVRAQDPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHG
HVRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSLNEASSGLFDVFLRF
MCHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAGIRRDGLLLRLVDDFL
LVTPHLTHAKTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEALGGTAFVQMPAHGLFPW
CGLLLDTRTLEVQSDYSSYARTSIRASLTFNRGFKAGRNMRRKLFGVLRLKCHSLFLDL
QVNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPTFFLRVISDTASLCYSILK
AKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSRKL
PGTTLTALEAAANPALPSDFKTILD"
primer_bind complement(4841..4861)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 4846..5175
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 5026..5161
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5088..5107
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 5185..5784
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(5185..5204)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 5681..5701
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
primer_bind complement(7257..7273)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7257..7273)
/label=pBluescriptSK
/note="For pBluescript vector"
primer_bind complement(7305..7324)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(7306..7324)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(7331..7348)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(7331..7347)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7340..7362)
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind complement(7576..7595)
/label=F1ori-R
/note="F1 origin, reverse primer"
LTR 7810..8279
/note="long terminal repeat from Moloney murine leukemia
virus"
primer_bind complement(8409..8426)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8580..9168)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8660..8679)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9328..10188)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9951..9970
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10189..10293)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
61 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
121 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
181 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
241 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
301 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
361 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
421 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
481 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggcaagctg
541 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
601 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
661 gttctgaaca cccggccgca accctgggag acgtcccagg gactttgggg gccgtttttg
721 tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat atgtggttct
781 ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg
841 gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt gttgtctctg
901 tctgactgtg tttctgtatt tgtctgaaaa ttagggccag actgttacca ctcccttaag
961 tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt cggtagatgt
1021 caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta acgtcggatg
1081 gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca aggtcttttc
1141 acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg aagccttggc
1201 ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc ctcctcttcc
1261 tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc gatcctccct
1321 ttatccagcc ctcactcctt ctctaggcgc cggccggatc ccagtgtggt ggtacgtagg
1381 aattcgtcct gctgcgcacg tgggaagccc tggccccggc cacccccgcg atgccgcgcg
1441 ctccccgctg ccgagccgtg cgctccctgc tgcgcagcca ctaccgcgag gtgctgccgc
1501 tggccacgtt cgtgcggcgc ctggggcccc agggctggcg gctggtgcag cgcggggacc
1561 cggcggcttt ccgcgcgctg gtggcccagt gcctggtgtg cgtgccctgg gacgcacggc
1621 cgccccccgc cgccccctcc ttccgccagg tgtcctgcct gaaggagctg gtggcccgag
1681 tgctgcagag gctgtgcgag cgcggcgcga agaacgtgct ggccttcggc ttcgcgctgc
1741 tggacggggc ccgcgggggc ccccccgagg ccttcaccac cagcgtgcgc agctacctgc
1801 ccaacacggt gaccgacgca ctgcggggga gcggggcgtg ggggctgctg ctgcgccgcg
1861 tgggcgacga cgtgctggtt cacctgctgg cacgctgcgc gctctttgtg ctggtggctc
1921 ccagctgcgc ctaccaggtg tgcgggccgc cgctgtacca gctcggcgct gccactcagg
1981 cccggccccc gccacacgct agtggacccc gaaggcgtct gggatgcgaa cgggcctgga
2041 accatagcgt cagggaggcc ggggtccccc tgggcctgcc agccccgggt gcgaggaggc
2101 gcgggggcag tgccagccga agtctgccgt tgcccaagag gcccaggcgt ggcgctgccc
2161 ctgagccgga gcggacgccc gttgggcagg ggtcctgggc ccacccgggc aggacgcgtg
2221 gaccgagtga ccgtggtttc tgtgtggtgt cacctgccag acccgccgaa gaagccacct
2281 ctttggaggg tgcgctctct ggcacgcgcc actcccaccc atccgtgggc cgccagcacc
2341 acgcgggccc cccatccaca tcgcggccac cacgtccctg ggacacgcct tgtcccccgg
2401 tgtacgccga gaccaagcac ttcctctact cctcaggcga caaggagcag ctgcggccct
2461 ccttcctact cagctctctg aggcccagcc tgactggcgc tcggaggctc gtggagacca
2521 tctttctggg ttccaggccc tggatgccag ggactccccg caggttgccc cgcctgcccc
2581 agcgctactg gcaaatgcgg cccctgtttc tggagctgct tgggaaccac gcgcagtgcc
2641 cctacggggt gctcctcaag acgcactgcc cgctgcgagc tgcggtcacc ccagcagccg
2701 gtgtctgtgc ccgggagaag ccccagggct ctgtggcggc ccccgaggag gaggacacag
2761 acccccgtcg cctggtgcag ctgctccgcc agcacagcag cccctggcag gtgtacggct
2821 tcgtgcgggc ctgcctgcgc cggctggtgc ccccaggcct ctggggctcc aggcacaacg
2881 aacgccgctt cctcaggaac accaagaagt tcatctccct ggggaagcat gccaagctct
2941 cgctgcagga gctgacgtgg aagatgagcg tgcggggctg cgcttggctg cgcaggagcc
3001 caggggttgg ctgtgttccg gccgcagagc accgtctgcg tgaggagatc ctggccaagt
3061 tcctgcactg gctgatgagt gtgtacgtcg tcgagctgct caggtctttc ttttatgtca
3121 cggagaccac gtttcaaaag aacaggctct ttttctaccg gaagagtgtc tggagcaagt
3181 tgcaaagcat tggaatcaga cagcacttga agagggtgca gctgcgggag ctgtcggaag
3241 cagaggtcag gcagcatcgg gaagccaggc ccgccctgct gacgtccaga ctccgcttca
3301 tccccaagcc tgacgggctg cggccgattg tgaacatgga ctacgtcgtg ggagccagaa
3361 cgttccgcag agaaaagagg gccgagcgtc tcacctcgag ggtgaaggca ctgttcagcg
3421 tgctcaacta cgagcgggcg cggcgccccg gcctcctggg cgcctctgtg ctgggcctgg
3481 acgatatcca cagggcctgg cgcaccttcg tgctgcgtgt gcgggcccag gacccgccgc
3541 ctgagctgta ctttgtcaag gtggatgtga cgggcgcgta cgacaccatc ccccaggaca
3601 ggctcacgga ggtcatcgcc agcatcatca aaccccagaa cacgtactgc gtgcgtcggt
3661 atgccgtggt ccagaaggcc gcccatgggc acgtccgcaa ggccttcaag agccacgtct
3721 ctaccttgac agacctccag ccgtacatgc gacagttcgt ggctcacctg caggagacca
3781 gcccgctgag ggatgccgtc gtcatcgagc agagctcctc cctgaatgag gccagcagtg
3841 gcctcttcga cgtcttccta cgcttcatgt gccaccacgc cgtgcgcatc aggggcaagt
3901 cctacgtcca gtgccagggg atcccgcagg gctccatcct ctccacgctg ctctgcagcc
3961 tgtgctacgg cgacatggag aacaagctgt ttgcggggat tcggcgggac gggctgctcc
4021 tgcgtttggt ggatgatttc ttgttggtga cacctcacct cacccacgcg aaaaccttcc
4081 tcaggaccct ggtccgaggt gtccctgagt atggctgcgt ggtgaacttg cggaagacag
4141 tggtgaactt ccctgtagaa gacgaggccc tgggtggcac ggcttttgtt cagatgccgg
4201 cccacggcct attcccctgg tgcggcctgc tgctggatac ccggaccctg gaggtgcaga
4261 gcgactactc cagctatgcc cggacctcca tcagagccag tctcaccttc aaccgcggct
4321 tcaaggctgg gaggaacatg cgtcgcaaac tctttggggt cttgcggctg aagtgtcaca
4381 gcctgtttct ggatttgcag gtgaacagcc tccagacggt gtgcaccaac atctacaaga
4441 tcctcctgct gcaggcgtac aggtttcacg catgtgtgct gcagctccca tttcatcagc
4501 aagtttggaa gaaccccaca tttttcctgc gcgtcatctc tgacacggcc tccctctgct
4561 actccatcct gaaagccaag aacgcaggga tgtcgctggg ggccaagggc gccgccggcc
4621 ctctgccctc cgaggccgtg cagtggctgt gccaccaagc attcctgctc aagctgactc
4681 gacaccgtgt cacctacgtg ccactcctgg ggtcactcag gacagcccag acgcagctga
4741 gtcggaagct cccggggacg acgctgactg ccctggaggc cgcagccaac ccggcactgc
4801 cctcagactt caagaccatc ctggactgag tcgaccctgt ggaatgtgtg tcagttaggg
4861 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
4921 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
4981 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
5041 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
5101 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
5161 ctaggctttt gcaaaaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc
5221 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
5281 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
5341 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
5401 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
5461 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
5521 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
5581 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
5641 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
5701 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
5761 atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg
5821 agcgcacgac cccatgcatc gataaaataa aagattttat ttagtctcca gaaaaagggg
5881 ggaatgaaag accccacctg taggtttggc aagctagacg gatcgggaga tcttcgcaaa
5941 acgctgggat tcccggatta caggcgggcg caccacacca ggagcaaaca cttccggttt
6001 taaaaattca gtttgtgatt ggctgtcatt cagtattatg ctaattaagc atgcccggtt
6061 ttaaacctct taaaacaact tttaaaatta cctttccacc taaaacgtta aaatttgtca
6121 agtgataata ttcgaaaagc tgttattgcc aaactatttt cctatttgtt tcctaatggc
6181 atcggaacta gcgaaagttt ctcgccatca gttaaaagtt tgcggcagat gtagacctag
6241 cagaggtgtg cgaggagaag cttgggttgc ggagggtggg cctgggaggg gtggtggcca
6301 ttttttgtct aaccctaact gagaagggcg taggcgccgt gcttttgctc cccgcgcgct
6361 gtttttctcg ctgactttca gcgggcggaa aagcctcggc ctgccgcctt ccaccgttca
6421 ttctagagca aacaaaaaat gtcagctgct ggcccgttcg cccctcccgg ggacctgcgg
6481 cgggtcgcct gcccagcccc cgaaccccgc ctggaggccg cggtcggccc ggggcttctc
6541 cggaggcacc cactgccacc gcgaagagtt gggctctgtc agccgcgggt ctctcggggg
6601 cgagggcgag gttcaggcct ttcaggccgc aggaagagga acggagcgag tccccgcgcg
6661 cggcgcgatt ccctgagctg tgggacgtgc acccaggact cggctcacac atgcagttcg
6721 ctttcctgtt ggtgggggga acgccgatcg tgcgcatccg tcacccctcg ccggcagtgg
6781 gggcttgtga acccccaaac ctgactgact gggccagtgt gctgcaaatt ggcaggagac
6841 gtgaaggcac ctccaaagtc ggccaaaatg aatgggcagt gagccggggt tgcctggagc
6901 cgttcctgcg tgggttctcc cgtcttccgc tttttgttgc cttttatggt tgtattacaa
6961 cttagttcct gctctgcaga ttttgttgag gtttttgctt ctcccaaggt agatctcgac
7021 cagtcccctc aacggggtgt gggagaacag tcattttttt ttgagagatc atttaacatt
7081 taatgaatat ttaattagaa gatctaaatg aacattggaa attgtgttcc tttaatggtc
7141 atcggtttat gccagaggtt agaagtttct tttttgaaaa attagacctt ggcgatgacc
7201 ttgagcagta ggatataacc cccacaagct tgatatcgaa ttcctgcagc ccgggggatc
7261 cactagttct agagcggccg ccaccgcggt ggagctccaa ttcgccctat agtgagtcgt
7321 attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
7381 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
7441 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
7501 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
7561 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
7621 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
7681 acctcgaccc caaaaaactt gattagggtg atggttcact agcttaagta acgccatttt
7741 gcaaggcatg gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag
7801 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
7861 cagggccaag aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca
7921 gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc
7981 agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg
8041 ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc
8101 gagctcaata aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag
8161 tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc
8221 gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcac
8281 atgcagcatg tatcaaaatt aatttggttt tttttcttaa gtatttacat taaatggcca
8341 tagttgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
8401 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
8461 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
8521 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
8581 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
8641 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
8701 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
8761 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
8821 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
8881 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
8941 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
9001 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
9061 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
9121 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
9181 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
9241 atgagattat caaaaaggat cttcacctag atccttttgc ggccgcaaat caatctaaag
9301 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
9361 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
9421 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
9481 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
9541 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
9601 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
9661 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
9721 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
9781 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
9841 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
9901 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
9961 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
10021 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
10081 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
10141 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
10201 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
10261 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgc
10321 agc
//
