| 质粒名称: | pBHt2G-RFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 107162 |
| 存储实验室: | Sophien Kamoun |
|
载体骨架基本信息 |
|
| 载体名称: | pBHt2G |
| 载体出品公司: | Khang et al, 2010 http://www.plantcell.org/content/22/4/1388 |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 9617 bp |
| 完整质粒大小: | -- |
| 载体修饰: | 去除BsaI位点来驯化克隆金门（GG）的主链。在大肠杆菌中添加一个用于红白选择的RFP标记。RFP两侧有两个BsaI站点。当基因插入时，GG反应导致RFP的丢失。RFP在大肠杆菌中表达，允许红白选择转化子。抗生素耐药性转为氨苄西林 |
| 载体类型: | 真菌土壤杆菌转化质粒，在Magnaporthe中测试 |
| 筛选标记: | 潮霉素 |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pBHt2G-RFP was a gift from Sophien Kamoun (Addgene plasmid # 107162 ; http://n2t.net/addgene:107162 ; RRID:Addgene_107162) |
买家导航
pBHt2GRFP载体基本信息
pBHt2GRFP载体图谱质粒图谱和多克隆位点信息
pBHt2GRFP载体简介
pBHt2GRFP载体序列
LOCUS pBHt2G-RFP 9623 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Golden-Gate compatible fungal transformation vector carrying
Hygromycin Resistance w RFP screening cassette (RFP knocked out by
GG reaction, allowing for red-white screening).
ACCESSION .
VERSION .
KEYWORDS pBHt2G-RFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9623)
TITLE pBHt2G-RFP
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 9623)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9623
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 99..120
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 135..165
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 173..189
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 178..200
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 197..213
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 197..213
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
protein_bind 344..365
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 380..410
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 418..434
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 452..463
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 470..1147
/codon_start=1
/product="monomeric derivative of DsRed (Campbell et al.,
2002)"
/label=mRFP1
/translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
TGA"
terminator 1192..1263
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 1279..1306
/label=T7Te terminator
/note="phage T7 early transcription terminator"
primer_bind complement(1345..1362)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(1345..1361)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1354..1376)
/label=M13/pUC Forward
/note="In lacZ gene"
misc_feature 1564..1588
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 2889..3518
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 3947..5020
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
rep_origin 5086..5280
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
primer_bind complement(5516..5538)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 5624..5764
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 5679..5698
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(5779..5796)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5950..6538)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6030..6049)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6625..7485)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7248..7267
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
misc_feature 7910..7934
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal 8012..8186
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(8217..9242)
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANILFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
ORIGIN
1 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
61 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
121 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
181 ggataacaat ttcacacagg aaacagctat gacatgatta cgaattcgga gtgagaccga
241 tatcaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
301 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
361 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
421 tgagcggata acaatttcac acatactaga gaaagaggag aaatactaga tggcttcctc
481 cgaggacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg
541 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc
601 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca
661 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa
721 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgagg acggtggtgt
781 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact
841 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga
901 agcttccacc gaacgtatgt acccggagga cggtgctctg aaaggtgaaa tcaaaatgcg
961 tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa
1021 aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca
1081 caacgaggac tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg
1141 tgcttaataa cgctgatagt gctagtgtag atcgctacta gagccaggca tcaaataaaa
1201 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
1261 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta taggtctcac
1321 gctctgcagg catgcaagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa
1381 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
1441 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
1501 ctagagcagc ttgagcttgg atcagattgt cgtttcccgc cttcagttta aactatcagt
1561 gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg
1621 gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc
1681 acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt
1741 cggcttctga cgttcagtgc agccgtctta tgaaaacgac atgtcgcaca agtcctaagt
1801 tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg
1861 cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc
1921 cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg
1981 ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag
2041 gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac
2101 agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg
2161 catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac
2221 gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct
2281 aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg
2341 cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga
2401 aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg
2461 cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg
2521 tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga
2581 acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc
2641 gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg
2701 cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccagccagc
2761 ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac
2821 agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag
2881 gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca
2941 tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt
3001 ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg
3061 ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact
3121 tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag
3181 ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc
3241 tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg
3301 tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg
3361 ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg
3421 ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc
3481 aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat
3541 gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc
3601 aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc
3661 ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct
3721 gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat
3781 gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg
3841 tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgcct
3901 gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa
3961 accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa
4021 ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg
4081 gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc
4141 gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta
4201 tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa
4261 gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt
4321 ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt
4381 ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg
4441 cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa
4501 gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca
4561 gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat
4621 tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct
4681 agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca
4741 ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg
4801 cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag
4861 cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct
4921 gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta
4981 ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg
5041 gcaaattgcc ctagcagggg aaaaaggtcg aaaaggactc tttcctgtgg atagcacgta
5101 cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc
5161 gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt
5221 ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata
5281 actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg
5341 ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc
5401 tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg
5461 ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
5521 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
5581 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
5641 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact
5701 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
5761 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
5821 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
5881 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
5941 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
6001 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
6061 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
6121 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
6181 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
6241 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
6301 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
6361 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
6421 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
6481 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
6541 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
6601 gattttggtc atgcattcta ggtattacca atgcttaatc agtgaggcac ctatctcagc
6661 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
6721 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagaac cacgctcacc
6781 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
6841 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
6901 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
6961 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
7021 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
7081 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
7141 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
7201 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
7261 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
7321 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
7381 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
7441 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatttatt atttccttcc
7501 tcttttctac agtatttaaa gataccccaa gaagctaatt ataacaagac gaactccaat
7561 tcactgttcc ttgcattcta aaaccttaaa taccagaaaa cagctttttc aaagttgttt
7621 tcaaagttgg cgtataacat agtatcgacg gagccgattt tgaaaccgcg gtgatcacag
7681 gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat catccgtgtt
7741 tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg agcaaagtct
7801 gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg cctgtatcga
7861 gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat
7921 attgtggtgt aaacaaattg acgcttagac aacttaataa cacattgcgg acgtttttaa
7981 tgtactgaat taacgccgaa ttaattcggg ggatctggat tttagtactg gattttggtt
8041 ttaggaatta gaaattttat tgatagaagt attttacaaa tacaaataca tactaagggt
8101 ttcttatatg ctcaacacat gagcgaaacc ctataggaac cctaattccc ttatctggga
8161 actactcaca cattattatg gagaaactcg aaactggttc ccggtcggca tctactctat
8221 tcctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca
8281 cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg
8341 gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg
8401 ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagg
8461 cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata
8521 caagccaacc acggcctcca gaagaggatg ttggcgacct cgtattggga atccccgaac
8581 atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg
8641 gagccgaaat ccgcatgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc
8701 agctcatcga gagcctgtgc gacggacgca ctgacggtgt cgtccatcac agtttgccag
8761 tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg
8821 attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc
8881 gcatccatgg cctccgcgac cggctggaga acagcgggca gttcggtttc aggcaggtct
8941 tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc gctgaactcc
9001 ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg ataaacataa
9061 cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc acgccctcct
9121 acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag gtcggagacg
9181 ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc aggctttttc
9241 atttggatgc ttgggtagaa taggtaagtc agattgaatc tgaaataaag ggaggaaggg
9301 cgaacttaag aaggtatgac cgggtcgtcc acttaccttg cttgacaaac gcaccaagtt
9361 atcgtgcacc aagcagcaga tgataataat gtcctcgttc ctgtctgcta ataagagtca
9421 cacttcgagc gccgccgcta ctgctacaag tggggctgat ctgaccagtt gcctaaatga
9481 accatcttgt caaacgacac aaattttgtg ctcaccgcct ggacgactaa accaaaatag
9541 gcattcattg ttgacctcca ctagctccag ccaagcccaa aaaatgctcc ttcaatatca
9601 gttttggcaa gctgctctag cca
//
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pBHt2GRFP载体质粒应用举例
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