| 质粒名称: | pBR322-Pdp1_NTD-Cre |
|---|---|
| 目录编号: | 198274 |
| 存储实验室: | Feng Zhang |
|
载体骨架基本信息 |
|
| 载体名称: | pBR322 |
| 完整质粒大小: | 7937 bp |
| 载体类型: | Bacterial Expression |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Pdp1_NTD-Cre |
| 物种来源: | Photorhabdus asymbiotica |
| 克隆方法: | 限制性内切酶 |
| 5’测序引物 | 未知 |
| 3’测序引物 | 未知 |
|
文献引用方法 |
|
| 材料和方法部分: | pBR322-Pdp1_NTD-Cre was a gift from Feng Zhang (Addgene plasmid # 198274 ; http://n2t.net/addgene:198274 ; RRID:Addgene_198274) |
| 参考文献部分: | Programmable protein delivery with a bacterial contractile injection system. Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29. 10.1038/s41586-023-05870-7 PubMed 36991127 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pBR322-Pdp1_NTD-Cre 7937 bp ds-DNA circular SYN 22-MAR-2023
DEFINITION For loading Cre into PVCs; also contains PVC regulatory genes .
ACCESSION .
VERSION .
KEYWORDS pBR322-Pdp1_NTD-Cre
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7937)
AUTHORS Kreitz J, Zhang F
TITLE Programmable protein delivery with a bacterial contractile injection
system
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7937)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Mar 22, 2023 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7937
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 3463..3654
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
primer_bind complement(3648..3670)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 3756..3896
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 3811..3830
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(3911..3928)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4082..4670)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4162..4181)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4841..5701)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5464..5483
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5702..5806)
/gene="bla"
/label=AmpR promoter
primer_bind 5874..5892
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 5919..5947
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
CDS 6861..6884
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 6888..6908
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 6909..7937
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLQ
SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
GD"
ORIGIN
1 tatggatttt catgtcatca ggagaatatt caaaattctg tgtcacacaa aatattttac
61 agcgtaatca caccattaag aggggcatca aaataaatgg ttaactggga caatgttgaa
121 tttcattttt ataaggcatt gtccgtatta tccaccatcc agatcaacgg attcagatgg
181 tgtaaagaaa gcatatagct ggtaggtaaa attaagaagt gtagttttat aaagtattaa
241 tggagaacga tgaatttaaa gtaaatggag gaaggttagt ttcactatca aattacagga
301 ccaaaaatat ggagtattcc atcttctttg gtgaattaag tcttgaagac ttcaacaaat
361 attgcctgga actatgggcc tgtgaatggt attggagtat catttataac gactatttcg
421 ataggaaatt taaagtcttg ttgaaaatga ctaatcgtct aaaagtatgt ctggagaaat
481 aggtttaatc tctatattta acggttttat tttcatctat ttactaatgg taaatttttt
541 tactctttga atatattggt gagtttgtta tccccattag ttaccagggt attattaatg
601 agtgaaattc aattttattg atattaaagt caaatttaca aatgatgact ttattatttt
661 ttatttatta attttaaatt attttttgtt aatctttact ataaatgaat tttttgcttg
721 catggacttg atttttttat atataacatt gacaaatatt aattcatata tatgattgtt
781 atagtgagtt atttaattga ttcgactaac aatttcattg taatttttca agatagcagt
841 caattgctat aacaagcatc tttctcgtca aaggaaaatg tgtatggcct ataagcagca
901 acttgaaaga tgaaggatat aatgatgatt aacttaacgg agagtagata ttagaagtag
961 tagaaggaca atttaagact ttatacccat taaaacaaag gggtaaaata atgatgtttg
1021 atgaaaattt agaatgtgtt aatcacgaaa ttatcaatga aaaattagat acgaaaattc
1081 accttgctaa tccttatgcc ttttgggggg gaagggtaaa tgagaatata aatgggttaa
1141 ttaaacaaca ttctttaaga ggaactgact ttaataaaat ttttaaccgg aagataaatt
1201 tttttgtaaa tggaataaat aattgccccc gaaaaactcg gaatataaaa ccccctaatt
1261 aattatttaa aggaataaga gtttatttgc tcgtagagta attatgttcc attaatatgc
1321 taaattgctc agtaaagtta ttaacttttt ttcaacgcca gtgaataagt ctgttttatt
1381 aaataaggag ttcaggtgtt tatttcaaaa gaattatcaa gctttattgc tgtagctaaa
1441 aataaatcaa taaataaagc aacaacggaa ctttttatta cacagtcacc gatttgccga
1501 agcttaaaaa aactggaatt ttcattaggt gtaaaactct ttgtaagaaa aagccaggga
1561 ttggctttaa cagaagaagg agaatgctta tataataaag ttttgtcaat ttgtgaacgg
1621 cttcaagaaa tagaaggtga ctacaagaat gaaaaatcaa catacaatga tctatatcaa
1681 aaaaatatat gaggttctaa tataataagc ggttctagat aactaaagtc atctattacc
1741 aatatctgta tactcagtga aagttttgtc ttctaggtaa gttttcataa tagtgacata
1801 ccttattttt tcatctgggt gtctctcatc ctgaagaatg ggtgatatag ttgaaataac
1861 tcagttcgtt ttgtggttta cgatttttgg cggatcacaa aaatagggct gagtcccttc
1921 aagtgagttt tgaaatatac aattataccc ttcatccttt aagttgttgc tttgttggct
1981 gttttcattc acctcagtca catagttatc tatgctcctg aggattcgtt cacttgccgt
2041 cgtgctgcaa ctcgaaatct attgggtata tatgtaattt tcatattctg tttcatttta
2101 acggtattat ttaaatattt atatttagaa cattaggtta ttagaataga taattttagg
2161 taaatgtaat taattcaacc atcacggaga gtttatcagt cgaccgatgc ccttgagagc
2221 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat
2281 gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt
2341 cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg
2401 aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac gtttcggcga
2461 gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt
2521 cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg
2581 gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct
2641 tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc attggaccgc tgatcgtcac
2701 ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg taggcgccgc
2761 cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac
2821 ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat tggagccaat
2881 caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat atccatcgcg
2941 tccgccatct ccagcagccg cacgcggcgc atctcgggca gcgttgggtc ctggccacgg
3001 gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact
3061 ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg
3121 tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa
3181 acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg
3241 ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt
3301 tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac
3361 cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc
3421 attaccccca tgaacagaaa tcccccttac acggaggcat cagtgaccaa acaggaaaaa
3481 accgccctta acatggcccg ctttatcaga agccagacat taacgcttct ggagaaactc
3541 aacgagctgg acgcggatga acaggcagac atctgtgaat cgcttcacga ccacgctgat
3601 gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
3661 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
3721 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
3781 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
3841 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
3901 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
3961 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
4021 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
4081 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
4141 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
4201 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
4261 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
4321 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
4381 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
4441 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
4501 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
4561 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
4621 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
4681 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
4741 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
4801 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
4861 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
4921 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
4981 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
5041 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
5101 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag
5161 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
5221 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
5281 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
5341 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
5401 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
5461 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
5521 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
5581 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
5641 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
5701 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
5761 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
5821 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
5881 gtatcacgag gccctttcgt cttcaagaat tctcatgttt gacagcttat catcgataag
5941 ctttaatgcg gtagtttatc acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa
6001 tctaacaatg cgctcatcgt catcctcggc accgtcaccc tggatgctgt aggcataggc
6061 ttggttatgc cggtactgcc gggcctcttg cgggatatcg tccattccga cagcatcgcc
6121 agtcactatg gcgtgctgct agcgctatat gcgttgatgc aatttctatg cgcacccgtt
6181 ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc gctacttgga
6241 gccactatcg actacgcgat catggcgacc acacccgtcc tgtggatcca tttttttaat
6301 taaataaagg aattaatatt gatttgatag agcatgtaat caataacttc tatgaaaatg
6361 ttgaaaaaca atacacggaa acacttaact tacaattaaa ttgttaaagg agatgatgat
6421 aagaatggtc agtcaccaga taaaatgatc tctggtcaat tttaggaaag ctgaaaaaac
6481 ctgaccaaat gcgtcgtaaa ttctatgctt agaaagactc ggtgccagtg taatacctaa
6541 gatatggttt ttatttccat atctgttcga aaacttagaa ataacaagtt caacaataac
6601 cagggacttt aaatatgcct agatatgcta attatcagat aaaccccaaa cagaatatta
6661 aaaattcaca tgggaaatct tcatcgtcag atttttctag tgggtacctt tcattctcaa
6721 ataattcgct tgatgaccct tttattcggc agcaagttaa gagagaattt atttgggagg
6781 gacatatgaa ggagattgaa gaagcttcaa gattaggtgg ctctggtggt ggcggttctg
6841 gcggcggtgg ttctggcggt gattataagg acgatgacga taaggatccc aagaaaaagc
6901 gcaaggtatc caatttactg accgtacacc aaaatttgcc tgcattaccg gtcgatgcaa
6961 cgagtgatga ggttcgcaag aacctgatgg acatgttcag ggatcgccag gcgttttctg
7021 agcatacctg gaaaatgctt cagtccgttt gccggtcgtg ggcggcatgg tgcaagttga
7081 ataaccggaa atggtttccc gcagaacctg aagatgttcg cgattatctt ctatatcttc
7141 aggcgcgcgg tctggcagta aaaactatcc agcaacattt gggccagcta aacatgcttc
7201 atcgtcggtc cgggctgcca cgaccaagtg acagcaatgc tgtttcactg gttatgcggc
7261 ggatccgaaa agaaaacgtt gatgccggtg aacgtgcaaa acaggctcta gcgttcgaac
7321 gcactgattt cgaccaggtt cgttcactca tggaaaatag cgatcgctgc caggatatac
7381 gtaatctggc atttctgggg attgcttata acaccctgtt acgtatagcc gaaattgcca
7441 ggatcagggt taaagatatc tcacgtactg acggtgggag aatgttaatc catattggca
7501 gaacgaaaac gctggttagc accgctggtg tagagaaggc acttagcctg ggggtaacta
7561 aactggtcga gcgatggatt tccgtctctg gtgtagctga tgatccgaat aactacctgt
7621 tttgccgggt cagaaaaaat ggtgttgccg cgccatctgc caccagccag ctatcaactc
7681 gcgccctgga agggattttt gaagcaactc atcgattgat ttacggcgct aaggatgact
7741 ctggtcagag atacctggcc tggtctggac acagtgcccg tgtcggagcc gcgcgagata
7801 tggcccgcgc tggagtttca ataccggaga tcatgcaagc tggtggctgg accaatgtaa
7861 atattgtcat gaactatatc cgtaacctgg atagtgaaac aggggcaatg gtgcgcctgc
7921 tggaagatgg cgattaa
//
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