| 质粒名称: | pBSTNAV |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 45801 |
| 存储实验室: | Luc Ponchon |
|
载体骨架基本信息 |
|
| 载体名称: | pBSTNAV |
| 载体出品公司: | -- |
| 完整质粒大小: | 3025 bp |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄青霉素，100μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | XL1 Blue |
| 拷贝数: | -- |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pBSTNAV was a gift from Luc Ponchon (Addgene plasmid # 45801 ; http://n2t.net/addgene:45801 ; RRID:Addgene_45801) |
| 参考文献部分: | Co-expression of RNA-protein complexes in Escherichia coli and applications to RNA biology. Ponchon L, Catala M, Seijo B, El Khouri M, Dardel F, Nonin-Lecomte S, Tisne C. Nucleic Acids Res. 2013 Aug;41(15):e150. doi: 10.1093/nar/gkt576. Epub 2013 Jun 26. 10.1093/nar/gkt576 PubMed 23804766 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pBSTNAV 3025 bp ds-DNA circular SYN 12-JAN-2022
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pBSTNAV
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3025)
AUTHORS Ponchon L, Catala M, Seijo B, El Khouri M, Dardel F, Nonin-Lecomte
S, Tisne C
TITLE Co-expression of RNA-protein complexes in Escherichia coli and
applications to RNA biology.
JOURNAL Nucleic Acids Res. 2013 Aug;41(15):e150. doi: 10.1093/nar/gkt576.
Epub 2013 Jun 26.
PUBMED 23804766
REFERENCE 2 (bases 1 to 3025)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jan 12, 2022 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..3025
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 1..20
/label=T7
/note="T7 promoter, forward primer"
promoter 1..19
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter complement(215..233)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(254..270)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(254..270)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(267..289)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 278..294
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(302..332)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 347..368
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(485..502)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(656..1244)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(736..755)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(1415..2275)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 2038..2057
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(2276..2380)
/gene="bla"
/label=AmpR promoter
rep_origin complement(2406..2861)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2543..2564)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2755..2774
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 2988..3010
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 3002..3019
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 3003..3019
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 taatacgact cactataggg cgaattggag ctaaagcttg gagctccgga tcctaagccc
61 ccatcaagct taaaaaaaat ccttagcttt cgctaaggat ctgcagcagc tgcccgggaa
121 ttcagcgtta caagtattac acaaagtttt ttatgttgag aatatttttt tgatggggcg
181 acctcgaggg ggggcccggt accagctttt gttcccttta gtgagggtta atttcgagct
241 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
301 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
361 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
421 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
481 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
541 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
601 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
661 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
721 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
781 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
841 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
901 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
961 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
1021 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
1081 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
1141 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
1201 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
1261 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
1321 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
1381 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
1441 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
1501 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
1561 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
1621 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
1681 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
1741 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
1801 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
1861 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
1921 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
1981 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
2041 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
2101 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
2161 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
2221 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
2281 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
2341 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
2401 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
2461 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
2521 cgagatagag ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
2581 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
2641 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
2701 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
2761 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
2821 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
2881 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
2941 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
3001 ttgtaaaacg acggccagtg aattg
//
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