| 质粒名称: | pBT268_(pCA-ATG-intron-tTA2-iiTRE-tdT3Mycii) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 36880 |
| 存储实验室: | Liqun Luo |
|
载体骨架基本信息 |
|
| 载体名称: | pBluescript SK(+) |
| 载体出品公司: | Stratagene |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 3000 bp |
| 完整质粒大小: | 9142 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 30 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | tTA2,tetracycline transactivator 2 |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | 在tTA2起始密码子后插入loxP位点的β-珠蛋白内含子 |
| GenBank编号 | -- |
| 启动子 | CAG (chicken beta actin promoter and CMV enhancer) |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pBT268_(pCA-ATG-intron-tTA2-iiTRE-tdT3Mycii) was a gift from Liqun Luo (Addgene plasmid # 36880 ; http://n2t.net/addgene:36880 ; RRID:Addgene_36880) |
| 参考文献部分: | Extensions of MADM (Mosaic Analysis with Double Markers) in Mice. Tasic B, Miyamichi K, Hippenmeyer S, Dani VS, Zeng H, Joo W, Zong H, Chen-Tsai Y, Luo L. PLoS One. 2012;7(3):e33332. Epub 2012 Mar 27. 10.1371/journal.pone.0033332 PubMed 22479386 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS pBT268_(pCA-ATG-intron-tTA2-iiTRE-tdT3Mycii) 9142 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pBT268_(pCA-ATG-intron-tTA2-iiTRE-tdT3Mycii)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9142)
AUTHORS Tasic B, Miyamichi K, Hippenmeyer S, Dani VS, Zeng H, Joo W, Zong H,
Chen-Tsai Y, Luo L
TITLE Extensions of MADM (Mosaic Analysis with Double Markers) in Mice.
JOURNAL PLoS One. 2012;7(3):e33332. Epub 2012 Mar 27.
PUBMED 22479386
REFERENCE 2 (bases 1 to 9142)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9142
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin complement(6..461)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(143..164)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 355..374
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 588..610
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 602..619
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 603..619
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 626..645
/label=T7
/note="T7 promoter, forward primer"
promoter 626..644
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 669..685
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind 670..686
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
enhancer 791..1094
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1096..1373
/label=chicken beta-actin promoter
intron 1375..2392
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 2316..2338
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 2400..2419
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
regulatory 2472..2481
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
protein_bind 2498..2531
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
CDS 2651..3394
/codon_start=1
/product="improved tetracycline-controlled transactivator"
/label=tTA-Advanced
/note="In the Tet-Off(R) system, tTA-Advanced binds to the
Tet-responsive element and stimulates transcription only in
the absence of tetracycline or doxycycline."
/translation="SRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVK
NKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRP
TEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTD
SMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPAD
ALDDFDLDMLPADALDDFDLDMLPG"
polyA_signal 3516..3637
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind 3607..3626
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
protein_bind 4161..4431
/label=tetracycline response element
/bound_moiety="tetracycline repressor TetR"
/note="contains seven copies of the tetracycline operator
tetO"
promoter 4464..4502
/label=minimal CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 4499..4523
/label=LNCX
/note="Human CMV promoter, forward primer"
regulatory 4627..4636
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 4633..6060
/codon_start=1
/product="tandem dimeric (pseudo-monomeric) derivative of
DsRed (Shaner et al., 2004)"
/label=tdTomato
/note="mammalian codon-optimized"
/translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
RSEGRHHLFLYGMDELYK"
CDS 6070..6099
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 6106..6135
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 6142..6171
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
polyA_signal 6309..6390
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter complement(6957..6975)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(6996..7012)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6996..7012)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7009..7031)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7020..7036
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7044..7074)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7089..7110
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7227..7244)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7398..7986)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7478..7497)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8157..9017)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8780..8799
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9018..9122)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtaccgg
661 gccccccctc gaggtcgacg gtatcgataa gcttccctta attaaccgtt taaacaattc
721 tgcaggaatc tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
781 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
841 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
901 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
961 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
1021 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
1081 tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc
1141 cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg
1201 cggggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg cggggcgggg
1261 cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta
1321 tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcggggagtc
1381 gctgcgacgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg
1441 gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg
1501 ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct
1561 tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg
1621 tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg
1681 gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg
1741 tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt
1801 gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc
1861 tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc
1921 ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc
1981 gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg ccggcggctg
2041 tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg
2101 acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc
2161 tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct
2221 tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg
2281 ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg
2341 cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg
2401 caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgattt gataccgcgg
2461 gcccgggttc cgccaccatg gttggtatca aagatctata acttcgtata gcatacatta
2521 tacgaagtta tggttacaag acaggtttaa ggagaccaat agaaactggg catgtggaga
2581 cagagaagac tcttgggttt ctgataggca ctgactctct ctgcctattg gtctattttc
2641 ccacccttag tctagactgg acaagagcaa agtcataaac tctgctctgg aattactcaa
2701 tgaagtcggt atcgaaggcc tgacgacaag gaaactcgct caaaagctgg gagttgagca
2761 gcctaccctg tactggcacg tgaagaacaa gcgggccctg ctcgatgccc tggcaatcga
2821 gatgctggac aggcatcata cccacttctg ccccctggaa ggcgagtcat ggcaagactt
2881 tctgcggaac aacgccaagt cattccgctg tgctctcctc tcacatcgcg acggggctaa
2941 agtgcatctc ggcacccgcc caacagagaa acagtacgaa accctggaaa atcagctcgc
3001 gttcctgtgt cagcaaggct tctccctgga gaacgcactg tacgctctgt ccgccgtggg
3061 ccactttaca ctgggctgcg tattggagga tcaggagcat caagtagcaa aagaggaaag
3121 agagacacct accaccgatt ctatgccccc acttctgaga caagcaattg agctgttcga
3181 ccatcaggga gccgaacctg ccttcctttt cggcctggaa ctaatcatat gtggcctgga
3241 gaaacagcta aagtgcgaaa gcggcgggcc ggccgacgcc cttgacgatt ttgacttaga
3301 catgctccca gccgatgccc ttgacgactt tgaccttgat atgctgcctg ctgacgctct
3361 tgacgatttt gaccttgaca tgctccccgg atgagaattc tagataactg atcataatca
3421 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
3481 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
3541 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
3601 ctagttgtgg tttgtccaaa ctcatcaatg tatcttattt aaatggggac agcccccccc
3661 caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc
3721 ggggctccgc tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca
3781 gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt
3841 tgagcctgca gacacctggg gggatacggg gaaaaagctt taggctgagt gcacggggac
3901 agcccccccc caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag
3961 cgagccgccc ggggctccgc tccggtccgg cgctcccccc gcatccccga gccggcagcg
4021 tgcggggaca gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct
4081 cgctgctctt tgagcctgca gacacctggg gggatacggg gaaaaagctt taggctgaag
4141 atctgattcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
4201 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
4261 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
4321 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
4381 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
4441 gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg ggaggcctat ataagcagag
4501 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
4561 gaagacaccg ggaccgatcc agcctccgcg gccccgaatt cgagctcggt acccggggat
4621 cgggccgcca ccatggtgag taagggcgag gaggtcatca aagagttcat gcgcttcaag
4681 gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc
4741 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc
4801 ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac
4861 cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc
4921 gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac
4981 ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta
5041 atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc
5101 gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg
5161 gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg
5221 gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag
5281 cgctccgagg gccgccacca cctgttcctg gggcatggca ccggcagcac cggcagcggc
5341 agctccggca ccgcctcctc cgaggacaac aacatggccg tcatcaaaga gttcatgcgc
5401 ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc
5461 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc
5521 ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg
5581 aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg
5641 gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg
5701 caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc
5761 cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc
5821 gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccgctac
5881 ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac
5941 tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag
6001 tacgagcgct ccgagggccg ccaccacctg ttcctgtacg gcatggacga gctgtacaag
6061 agatccttag agcagaaact cattagcgag gaggacctga acagcgaaca gaaactcatt
6121 tccgaagagg atctcaactc cgagcagaag ctgatcagcg aggaggacct gagatcctga
6181 gaattcgacg atatctctag aggatcataa tcagccatac cacatttgta gaggttttac
6241 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
6301 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
6361 atttcacaaa taaagcattt ttttcactgc ctcgaatcac tagtggggac agcccccccc
6421 caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc
6481 ggggctccgc tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca
6541 gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt
6601 tgagcctgca gacacctggg gggatacggg gaaaaagctt taggctgagt gcacggggac
6661 agcccccccc caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag
6721 cgagccgccc ggggctccgc tccggtccgg cgctcccccc gcatccccga gccggcagcg
6781 tgcggggaca gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct
6841 cgctgctctt tgagcctgca gacacctggg gggatacggg gaaaaagctt taggctgagg
6901 cgcgcccaac tagttctaga gcggccgcca ccgcggtgga gctccagctt ttgttccctt
6961 tagtgagggt taatttcgag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
7021 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
7081 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
7141 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
7201 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
7261 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
7321 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
7381 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
7441 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
7501 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
7561 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
7621 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
7681 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
7741 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
7801 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
7861 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
7921 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
7981 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
8041 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
8101 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
8161 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
8221 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
8281 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
8341 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
8401 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
8461 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
8521 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
8581 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
8641 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
8701 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
8761 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
8821 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
8881 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
8941 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
9001 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
9061 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
9121 cgcacatttc cccgaaaagt gc
//
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