| 质粒名称: | pC1-mitoRexYFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 60246 |
| 存储实验室: | Vsevolod Belousov |
|
载体骨架基本信息 |
|
| 载体名称: | pC1 |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 3930 bp |
| 完整质粒大小: | 5523 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | mitoRexYFP |
| 物种来源: | Synthetic |
| 插入大小: | 1593 bp |
| 启动子 | CMV |
| 标签/融合蛋白: | Mitochondrial targeting signal （插入物上的N端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | NheI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | ggtgggaggtctatataag |
| 3' 测序引物 | cctctacaaatgtggtatgg |
|
文献引用方法 |
|
| 材料和方法部分: | pC1-mitoRexYFP was a gift from Vsevolod Belousov (Addgene plasmid # 60246 ; http://n2t.net/addgene:60246 ; RRID:Addgene_60246) |
| 参考文献部分: | Genetically encoded fluorescent indicator for imaging NAD/NADH ratio changes in different cellular compartments. Bilan DS, Matlashov ME, Gorokhovatsky AY, Schultz C, Enikolopov G, Belousov VV. Biochim Biophys Acta. 2013 Nov 25. pii: S0304-4165(13)00519-9. doi: 10.1016/j.bbagen.2013.11.018. 10.1016/j.bbagen.2013.11.018 PubMed 24286672 |
买家导航
pC1mitoRexYFP载体基本信息
pC1mitoRexYFP载体图谱质粒图谱和多克隆位点信息
pC1mitoRexYFP载体简介
pC1mitoRexYFP载体序列
LOCUS pC1-mitoRexYFP 5521 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Mitochondria-targeted genetically encoded fluorescent indicator for
measuring NAD/NADH ratio..
ACCESSION .
VERSION .
KEYWORDS pC1-mitoRexYFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5521)
AUTHORS Bilan DS, Matlashov ME, Gorokhovatsky AY, Schultz C, Enikolopov G,
Belousov VV
TITLE Genetically encoded fluorescent indicator for imaging NAD/NADH ratio
changes in different cellular compartments.
JOURNAL Biochim Biophys Acta. 2013 Nov 25. pii: S0304-4165(13)00519-9. doi:
10.1016/j.bbagen.2013.11.018.
PUBMED 24286672
REFERENCE 2 (bases 1 to 5521)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5521
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 2..305
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 306..509
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 460..480
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
CDS 538..612
/codon_start=1
/product="mitochondrial presequence of human cytochrome c
oxidase subunit VIII (Rizutto et al., 1989)"
/label=COX8 presequence
/translation="MSVLTPLLLRGLTGSARRLPVPRAK"
CDS 634..705
/codon_start=1
/product="mitochondrial presequence of human cytochrome c
oxidase subunit VIII (Rizutto et al., 1989)"
/label=COX8 presequence
/translation="SVLTPLLLRGLTGSARRLPVPRAK"
primer_bind 1203..1224
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(1333..1354)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1594..1613)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
polyA_signal 2250..2371
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2287..2306)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2341..2360
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(2378..2833)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2515..2536)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2727..2746
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2860..2964
/gene="bla"
/label=AmpR promoter
promoter 2966..3323
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(2989..3009)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 3174..3309
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3236..3255
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3358..4152
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3412..3431)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4022..4041
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(4340..4359)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 4384..4431
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4760..5348
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5249..5268
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
61 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
121 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
181 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
241 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
301 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
361 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
421 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
481 tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagcatg
541 tccgtcctga cgccgctgct gctgcggggc ttgacaggct cggcccggcg gctcccagtg
601 ccgcgcgcca agatccattc gttgggggat ctgtccgtcc tgacgccgct gctgctgcgg
661 ggcttgacag gctcggcccg gcggctccca gtgccgcgcg ccaagatcca ttcgttgggg
721 gatccaccgg tcgccaccat gaaggtcccc gaagcggcca tcagccgcct gatcacctac
781 ctccgcatcc tggaggagct ggaggcccag ggggttcacc ggacgagctc cgagcagctt
841 ggggagctcg cccaggtcac ggccttccag gtgcggaagg acctttccta cttcggctcc
901 tacgggacgc gcggggtggg gtacaccgtc cccgtcctga agcgggagct ccgccacatc
961 ctggggctca accgctccgc cggctacaac agcgacaacg tctatatcat ggccgacaag
1021 cagaagaacg gcatcaaggc caacttcaag atccgccaca acgtcgagga cggcagcgtg
1081 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
1141 gacaaccact acctgagctt ccagtccgtc ctgagcaaag accccaacga gaagcgcgat
1201 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctcccggcat ggacgagctg
1261 aacaacgtgg atggcggtag cggtggcacc ggcagcaagg gcgaggagct gttcaccggg
1321 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
1381 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagctgat ctgcaccacc
1441 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tcggctacgg cctgaagtgc
1501 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
1561 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
1621 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcggcttc
1681 aaggaggacg gcaacatcct ggggcacaag ctggagtaca acggcaccaa gtggggcctg
1741 tgcatcgtgg gcatgggccg gctcgggagc gccctggccg actacccggg gttcggggag
1801 agctttgagc tcaggggctt ctttgacgtg gacccggaga aggtgggccg cccggtgcgg
1861 ggcggggtca ttgagcacgt ggacctcctg ccccagcggg ttcccggccg cattgagatc
1921 gccctcctca ccgtgccccg ggaagcggcc cagaaggcgg cggacctgtt ggtggcggcg
1981 gggatcaagg ggatcctgaa cttcgccccg gtggtcctcg aggtgcccaa ggaggtggcg
2041 gtggagaacg tggacttctt ggcggggctc acgcggcttt ctttcgctat cctgaacccc
2101 aagtggcggg aagagatgat ggggtgaagc ggccgcgact ctagatcata atcagccata
2161 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
2221 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
2281 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
2341 gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg
2401 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
2461 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
2521 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
2581 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
2641 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
2701 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
2761 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
2821 ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
2881 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
2941 caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc
3001 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
3061 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
3121 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
3181 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
3241 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
3301 ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg
3361 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
3421 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
3481 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa
3541 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
3601 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
3661 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
3721 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
3781 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
3841 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc
3901 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
3961 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
4021 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
4081 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
4141 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
4201 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
4261 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
4321 accctagggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta
4381 tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg
4441 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat
4501 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc
4561 tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt
4621 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
4681 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
4741 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
4801 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
4861 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
4921 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
4981 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
5041 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
5101 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
5161 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
5221 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
5281 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
5341 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
5401 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc
5461 attagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
5521 c
//
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