pCMV-Mm.cargo(Cas9)

LOCUS       pCMV-Mm.cargo(Cas9)             10593 bp ds-DNA     circular SYN 14-SEP-2021
DEFINITION  Expresses mouse Peg10 cargoRNA encoding SpCas9.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-Mm.cargo(Cas9)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10593)
  AUTHORS   Segel M, Lash B, Song J, Ladha A, Liu CC, Jin X, Mekhedov SL, Macrae
            RK, Koonin EV, Zhang F
  TITLE     Mammalian retrovirus-like protein PEG10 packages its own mRNA and 
            can be pseudotyped for mRNA delivery.
  JOURNAL   Science. 2021 Aug 20;373(6557):882-889. doi: 
            10.1126/science.abg6155.
  PUBMED    34413232
REFERENCE   2  (bases 1 to 10593)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 14, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10593
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1050..1076
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1080..1100
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1110..5210
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5214..5234
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5950..5967)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5956..6180
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6226..6654
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6313..6332)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6523..6544
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6663..6683)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6668..6997
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6848..6983
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6910..6929
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7064..7858
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7118..7137)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7728..7747
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8032..8153
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8069..8088)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8123..8142
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8202..8218)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8202..8218)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8215..8237)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8226..8242
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8250..8280)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8295..8316
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8433..8450)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8604..9192)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8684..8703)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9363..10223)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9986..10005
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10224..10328)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10403..10422)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaaaccggat ttggcgcccc ccctcctagg atctctctat ataaggcaag
      721 cagtccggac tcccgataca cgcgtttcca acttgagttg gtgtgtgtcg aagaatcctg
      781 accaactacg acctggggag agcagccaac cgagaaggtc caccgagcct cgcctaggtc
      841 tgctgcgcgg gctgcggtcc ggagccttct ccggaccgcg gtcacccagt ggaccgggcc
      901 gtcgcgggac ccctcatcct tcgtggcatc gcagaggaat cctcgtgtgg aacaggcggg
      961 ttttaagaac caaaagacgc caaccacgag ggtcccagga tccagggctc cctccccagg
     1021 ggagtgaagc ccctctcacc gcagccatgt acccatacga tgttccagat tacgcttcgc
     1081 cgaagaaaaa gcgcaaggtc gaagcgtccg acaagaagta cagcatcggc ctggacatcg
     1141 gcaccaactc tgtgggctgg gccgtgatca ccgacgagta caaggtgccc agcaagaaat
     1201 tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa gaacctgatc ggagccctgc
     1261 tgttcgacag cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc agaagaagat
     1321 acaccagacg gaagaaccgg atctgctatc tgcaagagat cttcagcaac gagatggcca
     1381 aggtggacga cagcttcttc cacagactgg aagagtcctt cctggtggaa gaggataaga
     1441 agcacgagcg gcaccccatc ttcggcaaca tcgtggacga ggtggcctac cacgagaagt
     1501 accccaccat ctaccacctg agaaagaaac tggtggacag caccgacaag gccgacctgc
     1561 ggctgatcta tctggccctg gcccacatga tcaagttccg gggccacttc ctgatcgagg
     1621 gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt catccagctg gtgcagacct
     1681 acaaccagct gttcgaggaa aaccccatca acgccagcgg cgtggacgcc aaggccatcc
     1741 tgtctgccag actgagcaag agcagacggc tggaaaatct gatcgcccag ctgcccggcg
     1801 agaagaagaa tggcctgttc ggaaacctga ttgccctgag cctgggcctg acccccaact
     1861 tcaagagcaa cttcgacctg gccgaggatg ccaaactgca gctgagcaag gacacctacg
     1921 acgacgacct ggacaacctg ctggcccaga tcggcgacca gtacgccgac ctgtttctgg
     1981 ccgccaagaa cctgtccgac gccatcctgc tgagcgacat cctgagagtg aacaccgaga
     2041 tcaccaaggc ccccctgagc gcctctatga tcaagagata cgacgagcac caccaggacc
     2101 tgaccctgct gaaagctctc gtgcggcagc agctgcctga gaagtacaaa gagattttct
     2161 tcgaccagag caagaacggc tacgccggct acattgacgg cggagccagc caggaagagt
     2221 tctacaagtt catcaagccc atcctggaaa agatggacgg caccgaggaa ctgctcgtga
     2281 agctgaacag agaggacctg ctgcggaagc agcggacctt cgacaacggc agcatccccc
     2341 accagatcca cctgggagag ctgcacgcca ttctgcggcg gcaggaagat ttttacccat
     2401 tcctgaagga caaccgggaa aagatcgaga agatcctgac cttccgcatc ccctactacg
     2461 tgggccctct ggccagggga aacagcagat tcgcctggat gaccagaaag agcgaggaaa
     2521 ccatcacccc ctggaacttc gaggaagtgg tggacaaggg cgcttccgcc cagagcttca
     2581 tcgagcggat gaccaacttc gataagaacc tgcccaacga gaaggtgctg cccaagcaca
     2641 gcctgctgta cgagtacttc accgtgtata acgagctgac caaagtgaaa tacgtgaccg
     2701 agggaatgag aaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc gtggacctgc
     2761 tgttcaagac caaccggaaa gtgaccgtga agcagctgaa agaggactac ttcaagaaaa
     2821 tcgagtgctt cgactccgtg gaaatctccg gcgtggaaga tcggttcaac gcctccctgg
     2881 gcacatacca cgatctgctg aaaattatca aggacaagga cttcctggac aatgaggaaa
     2941 acgaggacat tctggaagat atcgtgctga ccctgacact gtttgaggac agagagatga
     3001 tcgaggaacg gctgaaaacc tatgcccacc tgttcgacga caaagtgatg aagcagctga
     3061 agcggcggag atacaccggc tggggcaggc tgagccggaa gctgatcaac ggcatccggg
     3121 acaagcagtc cggcaagaca atcctggatt tcctgaagtc cgacggcttc gccaacagaa
     3181 acttcatgca gctgatccac gacgacagcc tgacctttaa agaggacatc cagaaagccc
     3241 aggtgtccgg ccagggcgat agcctgcacg agcacattgc caatctggcc ggcagccccg
     3301 ccattaagaa gggcatcctg cagacagtga aggtggtgga cgagctcgtg aaagtgatgg
     3361 gccggcacaa gcccgagaac atcgtgatcg aaatggccag agagaaccag accacccaga
     3421 agggacagaa gaacagccgc gagagaatga agcggatcga agagggcatc aaagagctgg
     3481 gcagccagat cctgaaagaa caccccgtgg aaaacaccca gctgcagaac gagaagctgt
     3541 acctgtacta cctgcagaat gggcgggata tgtacgtgga ccaggaactg gacatcaacc
     3601 ggctgtccga ctacgatgtg gaccatatcg tgcctcagag ctttctgaag gacgactcca
     3661 tcgacaacaa ggtgctgacc agaagcgaca agaaccgggg caagagcgac aacgtgccct
     3721 ccgaagaggt cgtgaagaag atgaagaact actggcggca gctgctgaac gccaagctga
     3781 ttacccagag aaagttcgac aatctgacca aggccgagag aggcggcctg agcgaactgg
     3841 ataaggccgg cttcatcaag agacagctgg tggaaacccg gcagatcaca aagcacgtgg
     3901 cacagatcct ggactcccgg atgaacacta agtacgacga gaatgacaag ctgatccggg
     3961 aagtgaaagt gatcaccctg aagtccaagc tggtgtccga tttccggaag gatttccagt
     4021 tttacaaagt gcgcgagatc aacaactacc accacgccca cgacgcctac ctgaacgccg
     4081 tcgtgggaac cgccctgatc aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg
     4141 actacaaggt gtacgacgtg cggaagatga tcgccaagag cgagcaggaa atcggcaagg
     4201 ctaccgccaa gtacttcttc tacagcaaca tcatgaactt tttcaagacc gagattaccc
     4261 tggccaacgg cgagatccgg aagcggcctc tgatcgagac aaacggcgaa accggggaga
     4321 tcgtgtggga taagggccgg gattttgcca ccgtgcggaa agtgctgagc atgccccaag
     4381 tgaatatcgt gaaaaagacc gaggtgcaga caggcggctt cagcaaagag tctatcctgc
     4441 ccaagaggaa cagcgataag ctgatcgcca gaaagaagga ctgggaccct aagaagtacg
     4501 gcggcttcga cagccccacc gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg
     4561 gcaagtccaa gaaactgaag agtgtgaaag agctgctggg gatcaccatc atggaaagaa
     4621 gcagcttcga gaagaatccc atcgactttc tggaagccaa gggctacaaa gaagtgaaaa
     4681 aggacctgat catcaagctg cctaagtact ccctgttcga gctggaaaac ggccggaaga
     4741 gaatgctggc ctctgccggc gaactgcaga agggaaacga actggccctg ccctccaaat
     4801 atgtgaactt cctgtacctg gccagccact atgagaagct gaagggctcc cccgaggata
     4861 atgagcagaa acagctgttt gtggaacagc acaagcacta cctggacgag atcatcgagc
     4921 agatcagcga gttctccaag agagtgatcc tggccgacgc taatctggac aaagtgctgt
     4981 ccgcctacaa caagcaccgg gataagccca tcagagagca ggccgagaat atcatccacc
     5041 tgtttaccct gaccaatctg ggagcccctg ccgccttcaa gtactttgac accaccatcg
     5101 accggaagag gtacaccagc accaaagagg tgctggacgc caccctgatc caccagagca
     5161 tcaccggcct gtacgagaca cggatcgacc tgtctcagct gggaggcgac agccccaaga
     5221 agaagagaaa ggtggaggcc agctaataag gcatggacga gctgtacaag taacatcctg
     5281 cagtgctgcg tgaaacctgt catgtccttt gtagtctctg ccctcaactt gatcctgtgc
     5341 agcttctcaa tctatgactg tgtggtactg gaccttcaga ggcgcacaga gctcaagtca
     5401 gttttcgtct tgactgccac tttataagtt gacaggcctg ggttttactt gttaaaacct
     5461 ctcaccatct caatcacagg ctgccaagtg tctttacaaa gaagctgata caaacacagg
     5521 ccatgctgat ttcttacaga gggagagaag aggaagagaa gaagaaagag gaggaagagg
     5581 acatgacttg cccatatgct gggcacctta taaaggaagc cagacttttc ggtgcagtat
     5641 ggaaaggctt ccgtgattct cttgctgcac cccacgaaac ttcaccacct tcaaactcca
     5701 ttttcacggt tccgttaatt ttcaaggagc agcaactcga ctggttctct gctacatgaa
     5761 acacctcagc ttgaaaagga agtgctctct cagactgact tgtgagtgtg ccttcacatt
     5821 ctggtgcaaa tcatgcttaa gcttggtacc gagctcggat ccactagtcc agtgtggtgg
     5881 aattctgcag atatccagca cagtggcggc cgctcgagtc tagagggccc gtttaaaccc
     5941 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     6001 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     6061 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     6121 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     6181 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     6241 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     6301 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     6361 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     6421 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     6481 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     6541 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     6601 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     6661 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     6721 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     6781 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     6841 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     6901 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     6961 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     7021 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     7081 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     7141 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     7201 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     7261 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     7321 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     7381 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     7441 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     7501 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     7561 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
     7621 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     7681 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     7741 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     7801 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     7861 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     7921 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     7981 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     8041 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     8101 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     8161 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     8221 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     8281 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     8341 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     8401 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     8461 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     8521 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     8581 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     8641 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     8701 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     8761 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     8821 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     8881 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     8941 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     9001 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     9061 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     9121 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     9181 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     9241 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     9301 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     9361 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     9421 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     9481 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     9541 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     9601 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     9661 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     9721 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     9781 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     9841 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     9901 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     9961 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    10021 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    10081 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    10141 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    10201 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    10261 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    10321 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg
    10381 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
    10441 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
    10501 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
    10561 tgcttcgcga tgtacgggcc agatatacgc gtt
//