| 质粒名称: | pCMV delta R8.2 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 12263 |
| 存储实验室: | Didier Trono |
|
载体骨架基本信息 |
|
| 载体名称: | pCMVR8.2 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | 8128 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | HIV-1 GAG/POL, Tat and Rev |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AgeI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | pCMV delta R8.2 was a gift from Didier Trono (Addgene plasmid # 12263 ; http://n2t.net/addgene:12263 ; RRID:Addgene_12263) |
| 参考文献部分: | -- |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCMV delta R8.2 13454 bp ds-DNA circular SYN 02-JAN-2020
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pCMV delta R8.2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13454)
TITLE Trono Lab Packaging and Envelope Plasmids
REFERENCE 2 (bases 1 to 13454)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jan 2, 2020 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..13454
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 1..380
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 381..584
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 535..555
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 581..605
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 855..2357
/codon_start=1
/gene="gag"
/product="gag protein from human immunodeficiency virus 1"
/label=HIV-1 gag
/translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA
VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI
EEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKA
FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGP
IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
CDS 2150..5161
/codon_start=1
/gene="pol"
/product="pol protein from human immunodeficiency virus 1"
/label=HIV-1 pol
/translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
MAGDDCVASRQDED"
misc_feature 4846..4963
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
misc_feature 6532..6765
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 7288..7314
/codon_start=1
/product="nuclear export signal from the HIV Rev protein
(Fischer et al., 1995)"
/label=NES
/translation="LPPLERLTL"
promoter complement(8551..8569)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(8552..8569)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(8859..8878)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(9092..9111)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 9211..9233
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
promoter 9357..9461
/gene="bla"
/label=AmpR promoter
CDS 9462..10322
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(9680..9699)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 10493..11081
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 10982..11001
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 11235..11252
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(11322..11342)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 11327..11656
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 11507..11642
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 11569..11588
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
intron 12790..12855
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 12985..13005
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
ORIGIN
1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
661 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg
721 taagtaccgc ctatagagtc tataggccca cccccttggc ttcttatgcg acggatcgat
781 cccgtaataa gcttcgaggt ccgcggccgc gttgacgcgc acggcaagag gcgaggggcg
841 gcgactggtg agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgat
901 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
961 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
1021 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
1081 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
1141 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag aaaaaagcac
1201 agcaagcagc agctgacaca ggacacagca atcaggtcag ccaaaattac cctatagtgc
1261 agaacatcca ggggcaaatg gtacatcagg ccatatcacc tagaacttta aatgcatggg
1321 taaaagtagt agaagagaag gctttcagcc cagaagtgat acccatgttt tcagcattat
1381 cagaaggagc caccccacaa gatttaaaca ccatgctaaa cacagtgggg ggacatcaag
1441 cagccatgca aatgttaaaa gagaccatca atgaggaagc tgcagaatgg gatagagtgc
1501 atccagtgca tgcagggcct attgcaccag gccagatgag agaaccaagg ggaagtgaca
1561 tagcaggaac tactagtacc cttcaggaac aaataggatg gatgacacat aatccaccta
1621 tcccagtagg agaaatctat aaaagatgga taatcctggg attaaataaa atagtaagaa
1681 tgtatagccc taccagcatt ctggacataa gacaaggacc aaaggaaccc tttagagact
1741 atgtagaccg attctataaa actctaagag ccgagcaagc ttcacaagag gtaaaaaatt
1801 ggatgacaga aaccttgttg gtccaaaatg cgaacccaga ttgtaagact attttaaaag
1861 cattgggacc aggagcgaca ctagaagaaa tgatgacagc atgtcaggga gtggggggac
1921 ccggccataa agcaagagtt ttggctgaag caatgagcca agtaacaaat ccagctacca
1981 taatgataca gaaaggcaat tttaggaacc aaagaaagac tgttaagtgt ttcaattgtg
2041 gcaaagaagg gcacatagcc aaaaattgca gggcccctag gaaaaagggc tgttggaaat
2101 gtggaaagga aggacaccaa atgaaagatt gtactgagag acaggctaat tttttaggga
2161 agatctggcc ttcccacaag ggaaggccag ggaattttct tcagagcaga ccagagccaa
2221 cagccccacc agaagagagc ttcaggtttg gggaagagac aacaactccc tctcagaagc
2281 aggagccgat agacaaggaa ctgtatcctt tagcttccct cagatcactc tttggcagcg
2341 acccctcgtc acaataaaga taggggggca attaaaggaa gctctattag atacaggagc
2401 agatgataca gtattagaag aaatgaattt gccaggaaga tggaaaccaa aaatgatagg
2461 gggaattgga ggttttatca aagtaagaca gtatgatcag atactcatag aaatctgcgg
2521 acataaagct ataggtacag tattagtagg acctacacct gtcaacataa ttggaagaaa
2581 tctgttgact cagattggct gcactttaaa ttttcccatt agtcctattg agactgtacc
2641 agtaaaatta aagccaggaa tggatggccc aaaagttaaa caatggccat tgacagaaga
2701 aaaaataaaa gcattagtag aaatttgtac agaaatggaa aaggaaggaa aaatttcaaa
2761 aattgggcct gaaaatccat acaatactcc agtatttgcc ataaagaaaa aagacagtac
2821 taaatggaga aaattagtag atttcagaga acttaataag agaactcaag atttctggga
2881 agttcaatta ggaataccac atcctgcagg gttaaaacag aaaaaatcag taacagtact
2941 ggatgtgggc gatgcatatt tttcagttcc cttagataaa gacttcagga agtatactgc
3001 atttaccata cctagtataa acaatgagac accagggatt agatatcagt acaatgtgct
3061 tccacaggga tggaaaggat caccagcaat attccagtgt agcatgacaa aaatcttaga
3121 gccttttaga aaacaaaatc cagacatagt catctatcaa tacatggatg atttgtatgt
3181 aggatctgac ttagaaatag ggcagcatag aacaaaaata gaggaactga gacaacatct
3241 gttgaggtgg ggatttacca caccagacaa aaaacatcag aaagaacctc cattcctttg
3301 gatgggttat gaactccatc ctgataaatg gacagtacag cctatagtgc tgccagaaaa
3361 ggacagctgg actgtcaatg acatacagaa attagtggga aaattgaatt gggcaagtca
3421 gatttatgca gggattaaag taaggcaatt atgtaaactt cttaggggaa ccaaagcact
3481 aacagaagta gtaccactaa cagaagaagc agagctagaa ctggcagaaa acagggagat
3541 tctaaaagaa ccggtacatg gagtgtatta tgacccatca aaagacttaa tagcagaaat
3601 acagaagcag gggcaaggcc aatggacata tcaaatttat caagagccat ttaaaaatct
3661 gaaaacagga aagtatgcaa gaatgaaggg tgcccacact aatgatgtga aacaattaac
3721 agaggcagta caaaaaatag ccacagaaag catagtaata tggggaaaga ctcctaaatt
3781 taaattaccc atacaaaagg aaacatggga agcatggtgg acagagtatt ggcaagccac
3841 ctggattcct gagtgggagt ttgtcaatac ccctccctta gtgaagttat ggtaccagtt
3901 agagaaagaa cccataatag gagcagaaac tttctatgta gatggggcag ccaataggga
3961 aactaaatta ggaaaagcag gatatgtaac tgacagagga agacaaaaag ttgtccccct
4021 aacggacaca acaaatcaga agactgagtt acaagcaatt catctagctt tgcaggattc
4081 gggattagaa gtaaacatag tgacagactc acaatatgca ttgggaatca ttcaagcaca
4141 accagataag agtgaatcag agttagtcag tcaaataata gagcagttaa taaaaaagga
4201 aaaagtctac ctggcatggg taccagcaca caaaggaatt ggaggaaatg aacaagtaga
4261 taaattggtc agtgctggaa tcaggaaagt actattttta gatggaatag ataaggccca
4321 agaagaacat gagaaatatc acagtaattg gagagcaatg gctagtgatt ttaacctacc
4381 acctgtagta gcaaaagaaa tagtagccag ctgtgataaa tgtcagctaa aaggggaagc
4441 catgcatgga caagtagact gtagcccagg aatatggcag ctagattgta cacatttaga
4501 aggaaaagtt atcttggtag cagttcatgt agccagtgga tatatagaag cagaagtaat
4561 tccagcagag acagggcaag aaacagcata cttcctctta aaattagcag gaagatggcc
4621 agtaaaaaca gtacatacag acaatggcag caatttcacc agtactacag ttaaggccgc
4681 ctgttggtgg gcggggatca agcaggaatt tggcattccc tacaatcccc aaagtcaagg
4741 agtaatagaa tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc
4801 tgaacatctt aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg
4861 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
4921 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
4981 cagcagagat ccagtttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt
5041 agtaatacaa gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcatcag
5101 ggattatgga aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta
5161 acacatggaa aagattagta aaacaccata tgtatatttc aaggaaagct aaggactggt
5221 tttatagaca tcactatgaa agtactaatc caaaaataag ttcagaagta cacatcccac
5281 taggggatgc taaattagta ataacaacat attggggtct gcatacagga gaaagagact
5341 ggcatttggg tcagggagtc tccatagaat ggaggaaaaa gagatatagc acacaagtag
5401 accctgacct agcagaccaa ctaattcatc tgcactattt tgattgtttt tcagaatctg
5461 ctataagaaa taccatatta ggacgtatag ttagtcctag gtgtgaatat caagcaggac
5521 ataacaaggt aggatctcta cagtacttgg cactagcagc attaataaaa ccaaaacaga
5581 taaagccacc tttgcctagt gttaggaaac tgacagagga cagatggaac aagccccaga
5641 agaccaaggg ccacagaggg agccatacaa tgaatggaca ctagagcttt tagaggaact
5701 taagagtgaa gctgttagac attttcctag gatatggctc cataacttag gacaacatat
5761 ctatgaaact tacggggata cttgggcagg agtggaagcc ataataagaa ttctgcaaca
5821 actgctgttt atccatttca gaattgggtg tcgacatagc agaataggcg ttactcgaca
5881 gaggagagca agaaatggag ccagtagatc ctagactaga gccctggaag catccaggaa
5941 gtcagcctaa aactgcttgt accaattgct attgtaaaaa gtgttgcttt cattgccaag
6001 tttgtttcat gacaaaagcc ttaggcatct cctatggcag gaagaagcgg agacagcgac
6061 gaagagctca tcagaacagt cagactcatc aagcttctct atcaaagcag taagtagtac
6121 atgtaatgca acctataata gtagcaatag tagcattagt agtagcaata ataatagcaa
6181 tagttgtgtg gtccatagta atcatagaat ataggaaaat attaagacaa agaaaaatag
6241 acaggttaat tgatagacta atagaaagag cagaagacag tggcaatgag agtgaaggag
6301 aagtatcagc acttgtggag atgggggtgg aaatggggca ccatgctcct tgggatattg
6361 atgatctgta gttgagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
6421 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
6481 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
6541 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
6601 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
6661 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
6721 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
6781 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
6841 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
6901 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
6961 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
7021 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
7081 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
7141 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
7201 agagagagac agagacagat ccattcgatt agtgaacgga tccttggcac ttatctggga
7261 cgatctgcgg agcctgtgcc tcttcagcta ccaccgcttg agagacttac tcttgattgt
7321 aacgaggatt gtggaacttc tgggacgcag ggggtgggaa gccctcaaat attggtggaa
7381 tctcctacaa tattggagtc aggagctaaa gaatagtgct gttagcttgc tcaatgccac
7441 agccatagca gtagctgagg ggacagatag ggttatagaa gtagtacaag gagcttgtag
7501 agctattcgc cacataccta gaagaataag acagggcttg gaaaggattt tgctataaga
7561 tgggtggcaa gtggtcaaaa agtagtgtga ttggatggcc tactgtaagg gaaagaatga
7621 gacgagctga gccagcagca gatggggtgg gagcagtatc tcgagaccta gaaaaacatg
7681 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac
7741 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt
7801 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
7861 ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
7921 tccctgattg gcagaactac acaccagggc cagggatcag atatccactg acctttggat
7981 ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga
8041 acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat
8101 tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg
8161 agtacttcaa gaactgctga gcggccgccc cggtgacctt cagaccttgg cactggaggt
8221 ggcccggcag aagcgcggca tcgtggatca gtgctgcacc agcatctgct ctctctacca
8281 actggagaac tactgcaact aggcccacca ctaccctgtc cacccctctg caatgaataa
8341 aacctttgaa agagcactac aagttgtgtg tacatgcgtg catgtgcata tgtggtgcgg
8401 ggggaacatg agtggggctg gctggagtgg cgatgataag ctgtcaaaca tgagaattct
8461 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
8521 gtttcttagt ctagaattaa ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat
8581 gatacataag gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa
8641 ttgtgtagca tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag
8701 agtcggtttg gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat
8761 ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt
8821 ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga
8881 tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt
8941 ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc
9001 ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca
9061 taagggagag cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
9121 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
9181 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
9241 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
9301 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
9361 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
9421 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
9481 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
9541 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
9601 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
9661 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
9721 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
9781 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
9841 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
9901 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
9961 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
10021 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
10081 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
10141 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
10201 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
10261 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
10321 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
10381 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
10441 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
10501 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
10561 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
10621 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
10681 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
10741 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
10801 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
10861 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
10921 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
10981 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
11041 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
11101 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
11161 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
11221 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
11281 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg
11341 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
11401 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
11461 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
11521 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
11581 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
11641 cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac
11701 cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac
11761 tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt
11821 tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct
11881 gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat
11941 ggaaaggcat tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga
12001 actgggtatt cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg
12061 cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga
12121 cctggtggat accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt
12181 tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat
12241 cccgcaagat acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat
12301 ctccggtcgc taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag
12361 gcgggttaca atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc
12421 tgagcgaaac cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc
12481 gctttaatca cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc
12541 actggtatcg catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa
12601 tcctcgacgt ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat
12661 acgatacggt gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt
12721 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
12781 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
12841 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc
12901 tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact
12961 gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac
13021 tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct
13081 tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa
13141 aaatattctg taacctttat aagtaggcat aacagttata atcataacat actgtttttt
13201 cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc
13261 tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact
13321 agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
13381 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgttg ggctgcagga
13441 attcgagctc gccc
//
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