| 质粒名称: | pDHt/sk-PC |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 92125 |
| 存储实验室: | Zhihua Zhou |
|
载体骨架基本信息 |
|
| 载体名称: | pDHt/sk |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | CRISPR；里氏木霉表达 |
| 筛选标记: | 潮霉素 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Cas9 |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | Ppdc |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gibson克隆 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pDHt/sk-PC was a gift from Zhihua Zhou (Addgene plasmid # 92125 ; http://n2t.net/addgene:92125 ; RRID:Addgene_92125) |
| 参考文献部分: | Efficient genome editing in filamentous fungus Trichoderma reesei using the CRISPR/Cas9 system. Liu R, Chen L, Jiang Y, Zhou Z, Zou G. Cell Discov. 2015 May 12;1:15007. doi: 10.1038/celldisc.2015.7. eCollection 2015. 10.1038/celldisc.2015.7 PubMed 27462408 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pDHt/sk-PC 15460 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expression of codon-optimized Cas9 with Ppdc promoter in Trichoderma
reesei .
ACCESSION .
VERSION .
KEYWORDS pDHt/sk-PC
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 15460)
AUTHORS Liu R, Chen L, Jiang Y, Zhou Z, Zou G
TITLE Efficient genome editing in filamentous fungus Trichoderma reesei
using the CRISPR/Cas9 system.
JOURNAL Cell Discov. 2015 May 12;1:15007. doi: 10.1038/celldisc.2015.7.
eCollection 2015.
PUBMED 27462408
REFERENCE 2 (bases 1 to 15460)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..15460
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 3..23
/label=T3
/note="T3 promoter, forward primer"
promoter 5..23
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
CDS 427..1452
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIDKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
polyA_signal 1960..2134
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
misc_feature 2212..2236
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
CDS 2661..3455
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
rep_origin 3542..4130
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4031..4050
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 4284..4301
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 4316..4456
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(4382..4401)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 4542..4564
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
rep_origin 4800..4994
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
CDS complement(5060..6133)
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
CDS complement(6562..7191)
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
misc_feature 8492..8516
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
primer_bind 8704..8726
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 8718..8735
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 8719..8735
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
CDS 10279..14382
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 14395..14415
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
ORIGIN
1 gcgcaattaa ccctcactaa agggaacaaa agctggagct ccaccgcggt ggcggccgcg
61 acgttaactg atattgaagg agcacttttt gggcttggct ggagctagtg gaggtcaaca
121 atgaatgcct attttggttt agtcgtccag gcggtgagca caaaatttgt gtcgtttgac
181 aagatggttc atttaggcaa ctggtcagat cagccccact tgtagcagta gcggcggcgc
241 tcgaagtgtg actcttatta gcagacagga actaggacat tatcatcatc tgctgcttgg
301 tgcacgataa cttggtgcgt ttgtcaagca aggtaagtga acgacccggt cataccttct
361 taagttcgcc cttcctccct ttgtttcaga ttcaatctga cttacctatt ctacccaagc
421 atcgatatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgacaag
481 ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc
541 ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac
601 aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt
661 gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc
721 acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc
781 atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg
841 caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat
901 gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc
961 gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat
1021 ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc
1081 gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg
1141 ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga
1201 tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg
1261 gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga
1321 tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc
1381 gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg
1441 gcaaaggaat agagtagatg ccgaccggga tcgatccact taacgttact gaaatcatca
1501 aacagcttga cgaatctgga tataagatcg ttggtgtcga tgtcagctcc ggagttgaga
1561 caaatggtgt tcaggatctc gataagatac gttcatttgt ccaagcagca aagagtgcct
1621 tctagtgatt taatagctcc atgtcaacaa gaataaaacg cgtttcgggt ttacctcttc
1681 cagatacagc tcaactgcaa tgcattaatg cattggacct cgcaacccta gtacgccctt
1741 caggctccgg cgaagcagaa gaatagctta gcagagtcta ttttcatttt cgggagacga
1801 gatcaagcag atcaacggtc gtcaagagac ctacgagact gaggaatccg ctcttggctc
1861 cacgcgacta tatatttgtc tctaattgta ctttgacatg ctcctcttct ttactctgat
1921 agcttgacta tgaaaattcc gtcaccagcc ctgctcgagt ttctccataa taatgtgtga
1981 gtagttccca gataagggaa ttagggttcc tatagggttt cgctcatgtg ttgagcatat
2041 aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt
2101 cctaaaacca aaatccagta ctaaaatcca gatcccccga attaattcgg cgttaattca
2161 gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc
2221 acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc
2281 accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag
2341 gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg
2401 tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt
2461 gctgcctgtg atcaccgcgg tttcaaaatc ggctccgtcg atactatgtt atacgccaac
2521 tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa tgcaaggaac
2581 agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa atactgtaga
2641 aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg aaaaaactga
2701 tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag gtatataagc
2761 tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat aaagggacca
2821 cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag ctgcctgttc
2881 caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg agtgaggccg
2941 atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag attatcgagc
3001 tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt ccctatacga
3061 atagcttaga cagccgctta gccgaattgg attacttact gaataacgat ctggccgatg
3121 tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag ctgtatgatt
3181 ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac ctgggagaca
3241 gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg agaagcggca
3301 gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag gatatcgggg
3361 aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat tgggagaaaa
3421 taaaatatta tattttactg gatgaattgt tttagtacct agaatgcatg accaaaatcc
3481 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
3541 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
3601 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
3661 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
3721 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
3781 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
3841 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
3901 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
3961 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
4021 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
4081 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
4141 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
4201 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
4261 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
4321 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
4381 gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga
4441 ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
4501 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
4561 gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt gccttgatgt gggcgccggc
4621 ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag attgcctggc cgtaggccag
4681 ccatttttga gcggccagcg gccgcgatag gccgacgcga agcggcgggg cgtagggagc
4741 gcagcgaccg aagggtaggc gctttttgca gctcttcggc tgtgcgctgg ccagacagtt
4801 atgcacaggc caggcgggtt ttaagagttt taataagttt taaagagttt taggcggaaa
4861 aatcgccttt tttctctttt atatcagtca cttacatgtg tgaccggttc ccaatgtacg
4921 gctttgggtt cccaatgtac gggttccggt tcccaatgta cggctttggg ttcccaatgt
4981 acgtgctatc cacaggaaag agaccttttc gacctttttc ccctgctagg gcaatttgcc
5041 ctagcatctg ctccgtacat taggaaccgg cggatgcttc gccctcgatc aggttgcggt
5101 agcgcatgac taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg tactccggca
5161 ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc
5221 tgccactgcg ttcgtagatc gtcttgaaca accatctggc ttctgccttg cctgcggcgc
5281 ggcgtgccag gcggtagaga aaacggccga tgccgggatc gatcaaaaag taatcggggt
5341 gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc caatcagcta
5401 gctcgatctc gatgtactcc ggccgcccgg tttcgctctt tacgatcttg tagcggctaa
5461 tcaaggcttc accctcggat accgtcacca ggcggccgtt cttggccttc ttcgtacgct
5521 gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg tctttctgct
5581 ttccgccatc ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga cggaacacgc
5641 ggccgggctt gtctcccttc ccttcccggt atcggttcat ggattcggtt agatgggaaa
5701 ccgccatcag taccaggtcg taatcccaca cactggccat gccggccggc cctgcggaaa
5761 cctctacgtg cccgtctgga agctcgtagc ggatcacctc gccagctcgt cggtcacgct
5821 tcgacagacg gaaaacggcc acgtccatga tgctgcgact atcgcgggtg cccacgtcat
5881 agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc ctaatcgacg
5941 gcgcaccggc tgccggcggt tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc
6001 acgattcacc ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct
6061 tcaacttctc caccaggtca tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt
6121 tgcgaccgct cacgccgatt cctcgggctt gggggttcca gtgccattgc agggccggca
6181 ggcaacccag ccgcttacgc ctggccaacc gcccgttcct ccacacatgg ggcattccac
6241 ggcgtcggtg cctggttgtt cttgattttc catgccgcct cctttagccg ctaaaattca
6301 tctactcatt tattcatttg ctcatttact ctggtagctg cgcgatgtat tcagatagca
6361 gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg tgatcctccg
6421 ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg
6481 cagccttgct gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg cttttgctca
6541 ttttctcttt acctcattaa ctcaaatgag ttttgattta atttcagcgg ccagcgcctg
6601 gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc cggcggcggc
6661 agtgcctggg tagctcacgc gctgcgtgat acgggactca agaatgggca gctcgtaccc
6721 ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac
6781 aaaggccgct tgtagccttc catccgtgac ctcaatgcgc tgcttaacca gctccaccag
6841 gtcggcggtg gcccatatgt cgtaagggct tggctgcacc ggaatcagca cgaagtcggc
6901 tgccttgatc gcggacacag ccaagtccgc cgcctggggc gctccgtcga tcactacgaa
6961 gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac
7021 ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg gcactgccct ggggatcgga
7081 atcgactaac agaacatcgg ccccggcgag ttgcagggcg cgggctagat gggttgcgat
7141 ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg ataaccttca tgcgttcccc
7201 ttgcgtattt gtttatttac tcatcgcatc atatacgcag cgaccgcatg acgcaagctg
7261 ttttactcaa atacacatca cctttttaga cggcggcgct cggtttcttc agcggccaag
7321 ctggccggcc aggccgccag cttggcatca gacaaaccgg ccaggatttc atgcagccgc
7381 acggttgaga cgtgcgcggg cggctcgaac acgtacccgg ccgcgatcat ctccgcctcg
7441 atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct ggtgcggttt catgcttgtt
7501 cctcttggcg ttcattctcg gcggccgcca gggcgtcggc ctcggtcaat gcgtcctcac
7561 ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc ctcgctgcgc tcaagtgcgc
7621 ggtacagggt cgagcgatgc acgccaagca gtgcagccgc ctctttcacg gtgcggcctt
7681 cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg ggtgagggta gggcgggggc
7741 caaacttcac gcctcgggcc ttggcggcct cgcgcccgct ccgggtgcgg tcgatgatta
7801 gggaacgctc gaactcggca atgccggcga acacggtcaa caccatgcgg ccggccggcg
7861 tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc cgcgccggcc tcctggatgc
7921 gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag gcggtctagc ctggtcactg
7981 tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc cagctccggg cggtcgcgcc
8041 tggtgccggt gatcttctcg gaaaacagct tggtgcagcc ggccgcgtgc agttcggccc
8101 gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc atagcccagc aggccagcgg
8161 cggcgctctt gttcatggcg taatgtctcc ggttctagtc gcaagtattc tactttatgc
8221 gactaaaaca cgcgacaaga aaacgccagg aaaagggcag ggcggcagcc tgtcgcgtaa
8281 cttaggactt gtgcgacatg tcgttttcag aagacggctg cactgaacgt cagaagccga
8341 ctgcactata gcagcggagg ggttggatca aagtactttg atcccgaggg gaaccctgtg
8401 gttggcatgc acatacaaat ggacgaacgg ataaaccttt tcacgccctt ttaaatatcc
8461 gattattcta ataaacgctc ttttctctta ggtttacccg ccaatatatc ctgtcaaaca
8521 ctgatagttt aaactgaagg cgggaaacga caatctgatc caagctcaag ctgctctagc
8581 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
8641 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
8701 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttaggact tccagggcta
8761 cttggcgcgg cagaagccgc aggtgtcgag ccgggaggag ttttcgcagt ggctgtgccg
8821 cgcgcacaac gatgtcaacc ggaagctggg caagcccgag tttgactgct cgcgggtgga
8881 cgagcggtgg aggacggggt ggaaggacgg tcggtgcgac tgattggcca tgaattccat
8941 ctttcgagga cggacgaaga tactgtacga ttaatgaaag gagggagcat tcttcgactt
9001 gcggcaattg catgcacatg tacgattgga agcgcgggcg atgtattcgc aatcatgttt
9061 agaaggacgg cgtttggaaa cgttgggatg ctgttgaagc gttggaaaca ggggcaatta
9121 gaaacaccga gccagacaga gtcaatggta cgaggtcagc cagtatcatg acctgtgtgc
9181 gcatggtggc gagagattcc gagccatgcc acgggagacg agcaatgaaa aaactcttca
9241 ctcacttgtc gaggctctct caacctatcg acttatcaag tagacgatga aagccttgca
9301 actgtggtga tgtggctcat caatgtgcga cgtcgtatcc atgtctgagg ccattcgata
9361 tcgtgatgcg actacctagt aaagcccggc cagagggcaa accggggcga caggggcagg
9421 caattgaccg gatggctgca tgtgccgaag cagccccgat ggaatcgaga tgtctgtcgg
9481 atggaccgct gagcggcctg gcaaggtgtc ccagatacga agatggaagt gaagtcagag
9541 gtggtcgtta attgtccgac gagcgaatcg gccgctcctt cggattgccg gctctgctgt
9601 atgtaccgtg catgaagcca cccgggatcc atgttacgat ggataggttc caactctcta
9661 gtagctatag tggacctgag gctatctagt atcactggag gagcagccgt ccactatcgt
9721 cgagcgctgt agaagcagct gcattagcgg ctgcccaccc gcgcagaaat ggccccatta
9781 catcactatc atgacagcgg cgcgtccaaa agtgagctca tgcttgccga tggcacgagc
9841 agctgcaact ggcggggctc ctgcctgccg tctccggtgc cgctgcccat ttgagtttgt
9901 ccgagctgtt gatggttgaa accgagaccg atggatgatt caacacttcg aagtctaggt
9961 agataaaaaa catctatata tcctcattca ttgccctgtc agtgtgttgg ctcacgtctc
10021 caatcctccg cccctcctcc tgcaaagtaa ataccttctc aaaacacgtc tggaatcctg
10081 caagtctcca tcacaaggag cttcttcatc aaccacctta tacgagcaac atcatttgca
10141 tcatcgttga tccacatctc ctcgcgcctc agagtgtcgt caccagtata aataaccgca
10201 tcaagctctc gtccttcttc gttccacaat ccaagaagca cctcaaaacg atcaaagcag
10261 cgcagctaca gcacaatcat ggacaagaag tacagcattg gcctggacat tggcacgaac
10321 tcggtcggct gggccgtcat cacggacgag tacaaggtcc cctccaagaa gtttaaggtc
10381 ctgggcaaca ccgaccgcca ctccatcaag aagaacctca ttggcgccct gctcttcgac
10441 tccggcgaga ccgccgaggc cacccgcctc aagcgcaccg cccgccgccg atacacgcgc
10501 cgcaagaacc gcatctgcta cctgcaggag attttctcca acgagatggc caaggtcgac
10561 gactccttct ttcaccgcct ggaggagtcg ttcctcgtcg aggaagacaa gaagcacgag
10621 cgccacccca tctttggcaa cattgtcgac gaggtcgcct accacgagaa gtaccccacg
10681 atctaccacc tgcgcaagaa gctcgtcgac tccaccgaca aggccgacct ccgcctgatc
10741 tacctcgccc tggcccacat gattaagttc cgcggccact ttctgatcga gggcgacctc
10801 aaccccgaca acagcgacgt cgacaagctg ttcatccagc tcgtccagac ctacaaccag
10861 ctctttgagg agaaccccat taacgcctcc ggcgtcgacg ccaaggccat cctctcggcc
10921 cgcctctcca agagccgccg actcgagaac ctgatcgccc agctgcccgg cgagaagaag
10981 aacggcctgt tcggcaacct catcgccctc tccctgggcc tcacccccaa cttcaagtcg
11041 aactttgacc tcgccgagga cgccaagctg cagctctcca aggacaccta cgacgacgac
11101 ctggacaacc tcctggccca gatcggcgac cagtacgccg acctgttcct cgccgccaag
11161 aacctgtccg acgccatcct cctgtcggac attctccgcg tcaacaccga gattacgaag
11221 gcccctctct ccgcctcgat gatcaagcgc tacgacgagc accaccagga cctgaccctg
11281 ctcaaggccc tggtccgcca gcagctcccc gagaagtaca aggagatctt ctttgaccag
11341 agcaagaacg gctacgccgg ctacatcgac ggcggcgcta gccaagagga gttctacaag
11401 tttatcaagc ccattctgga gaagatggac ggcacggagg agctcctggt caagctcaac
11461 cgcgaggacc tcctgcgcaa gcagcgcacc ttcgacaacg gcagcatccc ccaccagatt
11521 cacctcggcg agctgcacgc catcctccgc cgacaagagg acttctaccc ctttctcaag
11581 gacaaccgcg agaagatcga gaagattctg acgttccgca tcccctacta cgtcggcccc
11641 ctggcccgcg gcaacagccg ctttgcctgg atgacccgca agtccgagga gaccatcacg
11701 ccctggaact tcgaggaagt cgtcgacaag ggcgcctcgg cccagtcctt catcgagcgc
11761 atgaccaact ttgacaagaa cctgcccaac gagaaggtcc tccccaagca ctcgctcctg
11821 tacgagtact tcaccgtcta caacgagctc acgaaggtca agtacgtcac cgagggcatg
11881 cgcaagcccg ccttcctgtc gggcgagcag aagaaggcca tcgtcgacct cctgtttaag
11941 accaaccgca aggtcacggt caagcagctc aaggaagact acttcaagaa gattgagtgc
12001 tttgacagcg tcgagatctc cggcgtcgag gaccgcttta acgcctccct gggcacctac
12061 cacgacctcc tgaagatcat taaggacaag gacttcctgg acaacgagga gaacgaggac
12121 atcctcgagg acattgtcct gaccctcacg ctgtttgagg accgcgagat gatcgaggag
12181 cgcctgaaga cgtacgccca cctcttcgac gacaaggtca tgaagcagct caagcgccgc
12241 cgatacaccg gctggggccg cctgagccgc aagctcatca acggcattcg cgacaagcag
12301 tcgggcaaga cgatcctcga cttcctgaag agcgacggct tcgccaaccg caactttatg
12361 cagctgattc acgacgactc cctcaccttc aaggaagaca tccagaaggc ccaggtctcc
12421 ggccagggcg actccctgca cgagcacatc gccaacctcg ccggcagccc cgccatcaag
12481 aagggcattc tgcagaccgt caaggtcgtc gacgagctcg tcaaggtcat gggccgccac
12541 aagcccgaga acatcgtcat tgagatggcc cgcgagaacc agaccacgca gaagggccag
12601 aagaacagcc gcgagcgcat gaagcgcatc gaggaaggca tcaaggagct gggctcccag
12661 atcctcaagg agcaccccgt cgagaacacc cagctgcaga acgagaagct ctacctgtac
12721 tacctccaga acggccgcga catgtacgtc gaccaggagc tggacattaa ccgcctctcg
12781 gactacgacg tcgaccacat cgtcccccag agcttcctga aggacgactc catcgacaac
12841 aaggtcctca cccgcagcga caagaaccgc ggcaagagcg acaacgtccc ctccgaggaa
12901 gtcgtcaaga agatgaagaa ctactggcgc cagctcctga acgccaagct gatcacgcag
12961 cgcaagtttg acaacctcac caaggccgag cgaggcggcc tctcggagct ggacaaggcc
13021 ggcttcatca agcgccagct ggtcgagacc cgccagatca cgaagcacgt cgcccagatt
13081 ctcgactcgc gcatgaacac gaagtacgac gagaacgaca agctgatccg cgaggtcaag
13141 gtcattaccc tgaagtcgaa gctcgtcagc gacttccgca aggacttcca gttttacaag
13201 gtccgcgaga tcaacaacta ccaccacgcc cacgacgcct acctcaacgc cgtcgtcggc
13261 accgccctga tcaagaagta ccccaagctc gagtccgagt tcgtctacgg cgactacaag
13321 gtctacgacg tccgcaagat gatcgccaag tccgagcagg agattggcaa ggccaccgcc
13381 aagtacttct tttactcgaa catcatgaac ttctttaaga ccgagatcac cctcgccaac
13441 ggcgagatcc gcaagcgccc cctcattgag accaacggcg agaccggcga gatcgtctgg
13501 gacaagggcc gcgacttcgc caccgtccgc aaggtcctca gcatgcccca ggtcaacatc
13561 gtcaagaaga ccgaggtcca gacgggcggc ttctcgaagg agagcattct gcccaagcgc
13621 aactccgaca agctcatcgc ccgcaagaag gactgggacc ccaagaagta cggtggcttc
13681 gactccccca ccgtcgccta ctcggtcctg gtcgtcgcca aggtcgagaa gggcaagtcg
13741 aagaagctca agagcgtcaa ggagctcctg ggcatcacca ttatggagcg cagctccttc
13801 gagaagaacc ccatcgactt tctcgaggcc aagggctaca aggaagtcaa gaaggacctg
13861 atcattaagc tccccaagta ctccctcttc gagctggaga acggccgcaa gcgcatgctc
13921 gcctccgccg gcgagctcca gaagggcaac gagctcgccc tgcccagcaa gtacgtcaac
13981 ttcctctacc tggccagcca ctacgagaag ctcaagggct cccccgagga caacgagcag
14041 aagcagctgt ttgtcgagca gcacaagcac tacctcgacg agatcattga gcagatttcc
14101 gagttctcga agcgcgtcat cctggccgac gccaacctgg acaaggtcct cagcgcctac
14161 aacaagcacc gcgacaagcc catccgcgag caggccgaga acatcattca cctcttcacc
14221 ctgaccaacc tcggcgcccc cgccgccttc aagtactttg acaccacgat cgaccgcaag
14281 cgctacacct cgacgaagga agtcctggac gccaccctca tccaccagag cattaccggc
14341 ctctacgaga cgcgcatcga cctcagccag ctcggcggcg actcccgcgc cgaccccaag
14401 aagaagcgca aggtctaacc cggcatgaag tctgaccggg tagtatgagg gttcatcgtc
14461 accttgatag aataatagac gataaagcag gccacgggca ggtaccgatt gtcaatccgg
14521 caggttcgga ggcgtgttgg aaatgagttt atgggttatg gtcaaatcgg atagtatgag
14581 gtacatagtt tgtaaatctc aagattattt tcttccttaa tcttgcacgt cgcatgagag
14641 ggaccgagaa gagaattgat gaagggctct tgaagatgag atgaatcacg tggttgctga
14701 agcttcagta gtctcgggta cctgttcttt cccacaaaca gtagccaggc tagaggtact
14761 gagtacccgc tcaccgtatc taatcatccg acctgaaatc ttcaagctgt tttattgaca
14821 cttcgagtcc atcttcattc acgtaaggag aacttctagg acatcactta tcccgccata
14881 tttagctgca aggagtcaat tgcaatgtca gattccgctc ctaagaggaa acagggccct
14941 ggcggctcag atggctcggc attgaagaag agaaaggtat gatgacaaga atgcttcttt
15001 tacatattta cgaagaatgc caccaccctt ctctctggct acctatgtcc atggacaacg
15061 agcttgctga caatgggact atgaatgggt acctaggagg gcacgggagg aagatggaag
15121 acgccagctc aaaaggcaaa gcaggcctcg tgggtcgaga tgggcaaggc tctcgaggtg
15181 ggcgacgaag gcatctgggt cacgtatgcg agaggcatga agaacaaggc cgttcgggag
15241 tttacggcca tgtgccatga ggtaaagctg cataatccca tgcccaccca cggggagact
15301 attttagatg tttattggtt gcggcgggga tgataataac gatgttggca gtacggcgaa
15361 aagctctacg ggatcacgcc cccggccgac gagtcagaag ctcaccaccc ggccgacgga
15421 gccgaagagg aagacatcga ggcgtccatc tagaggatcc
//
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