| 质粒名称: | pET-NtLAtC60αβC20 |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 160873 |
| 存储实验室: | Maureen Hanson |
|
载体骨架基本信息 |
|
| 载体名称: | pET-11a |
| 完整质粒大小: | 10867 bp |
| 载体类型: | 细菌细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | rbcL, cpn60α, cpn60β, cpn20 |
| 物种来源: | A. thaliana (mustard weed); Nicotiana tabacum |
| 启动子: | rbcL: pBAD, chaperones: T7 |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | 未知(未破坏) |
|
文献引用方法 |
|
| 材料和方法部分: | pET-NtLAtC60αβC20 was a gift from Maureen Hanson (Addgene plasmid # 160873 ; http://n2t.net/addgene:160873 ; RRID:Addgene_160873) |
| 参考文献部分: | Small subunits can determine enzyme kinetics of tobacco Rubisco expressed in Escherichia coli. Lin MT, Stone WD, Chaudhar Vi, Hanson MR. Nat Plants 10.1038/s41477-020-00761-5 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pET-NtLAtC60αβC20 10867 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION Expression of N. tabacum Rubisco large subunit and A. thaliana
Rubisco chaperone protiens cpn60-alpha, cpn60-beta, cpn20 in E.
coli.
ACCESSION .
VERSION .
KEYWORDS pET-NtLAtC60-alpha-beta-C20
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10867)
AUTHORS Lin MT, Stone WD, Chaudhar Vi, Hanson MR
TITLE Small subunits can determine enzyme kinetics of tobacco Rubisco
expressed in Escherichia coli
JOURNAL Nat Plants
REFERENCE 2 (bases 1 to 10867)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..10867
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(43..61)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 129..233
/gene="bla"
/label=AmpR promoter
CDS 234..1094
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(452..471)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1265..1853
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 1754..1773
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 2007..2024
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 2039..2178
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(2104..2123)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 2264..2286
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
CDS complement(2280..2471)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
CDS complement(3203..4081)
/codon_start=1
/gene="araC"
/product="L-arabinose regulatory protein"
/label=araC
/translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
EKVNDVAVKLS"
promoter 4108..4392
/gene="araBAD"
/label=araBAD promoter
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
primer_bind 4315..4334
/label=pBAD Forward
/note="For vectors with E. coli araBAD promoter, forward
primer"
RBS 4419..4441
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 5905..5946
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
CDS 5956..5973
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(6029..6046)
/label=pBAD Reverse
/note="For vectors with E. coli araBAD promoter, reverse
primer"
terminator 6199..6285
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 6377..6404
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
primer_bind 6457..6476
/label=T7
/note="T7 promoter, forward primer"
promoter 6457..6475
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 6476..6500
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 6515..6537
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
RBS 8186..8208
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
RBS 8626..8634
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
RBS 9901..9923
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
primer_bind complement(10615..10633)
/label=T7 Term
/note="T7 terminator, reverse primer"
terminator 10629..10676
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
ORIGIN
1 gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat
61 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
121 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
181 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
241 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
301 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
361 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
421 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
481 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
541 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
601 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
661 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
721 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag
781 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
841 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
901 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
961 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
1021 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
1081 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
1141 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
1201 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
1261 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
1321 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
1381 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
1441 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
1501 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
1561 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
1621 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
1681 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
1741 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
1801 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
1861 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
1921 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
1981 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
2041 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcaat ggtgcactct
2101 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt
2161 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
2221 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
2281 cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg
2341 tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc
2401 tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc
2461 tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg
2521 atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg
2581 gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact
2641 cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag
2701 catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga
2761 ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg
2821 cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg
2881 caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc
2941 caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg
3001 gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct
3061 ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcgaagcgg catgcataat
3121 gtgcctgtca aatggacgaa gcagggattc tgcaaaccct atgctactcc gtcaagccgt
3181 caattgtctg attcgttacc aattatgaca acttgacggc tacatcattc actttttctt
3241 cacaaccggc acggaactcg ctcgggctgg ccccggtgca ttttttaaat acccgcgaga
3301 aatagagttg atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtgg
3361 tgctcaaaag cagcttcgcc tggctgatac gttggtcctc gcgccagctt aagacgctaa
3421 tccctaactg ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg
3481 cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct
3541 cgcgtacccg attatccatc ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca
3601 gtaacaattg ctcaagcaga tttatcgcca gcagctccga atagcgccct tccccttgcc
3661 cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc
3721 gaaagaaccc cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc
3781 gcggacgaaa gtaaacccac tggtgatacc attcgcgagc ctccggatga cgaccgtagt
3841 gatgaatctc tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc
3901 cctgattttt caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc
3961 ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc gttaaacccg
4021 ccaccagatg ggcattaaac gagtatcccg gcagcagggg atcattttgc gcttcagcca
4081 tacttttcat actcccgcca ttcagagaag aaaccaattg tccatattgc atcagacatt
4141 gccgtcactg cgtcttttac tggctcttct cgctaaccaa accggtaacc ccgcttatta
4201 aaagcattct gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct
4261 ataatcacgg cagaaaagtc cacattgatt atttgcacgg cgtcacactt tgctatgcca
4321 tagcattttt atccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta
4381 ctgtttctcc atacccgttt ttttgggcta gaaataattt tgtttaactt taagaaggag
4441 atatacatac ccatgtcacc acaaacagag actaaagcaa gtgttggatt caaagctggt
4501 gttaaagagt acaaattgac ttattatact cctgagtacc aaaccaagga tactgatata
4561 ttggcagcat tccgagtaac tcctcaacct ggagttccac ctgaagaagc aggggccgcg
4621 gtagctgccg aatcttctac tggtacatgg acaactgtat ggaccgatgg acttaccagc
4681 cttgatcgtt acaaagggcg atgctaccgc atcgagcgtg ttgttggaga aaaagatcaa
4741 tatattgctt atgtagctta ccctttagac ctttttgaag aaggttctgt taccaacatg
4801 tttacttcca ttgtaggtaa cgtatttggg ttcaaagccc tgcgcgctct acgtctggaa
4861 gatctgcgaa tccctcctgc ttatgttaaa actttccaag gtccgcctca tgggatccaa
4921 gttgaaagag ataaattgaa caagtatggt cgtcccctgt tgggatgtac tattaaacct
4981 aaattggggt tatctgctaa aaactacggt agagctgttt atgaatgtct tcgcggtgga
5041 cttgatttta ccaaagatga tgagaacgtg aactcacaac catttatgcg ttggagagat
5101 cgtttcttat tttgtgccga agcactttat aaagcacagg ctgaaacagg tgaaatcaaa
5161 gggcattact tgaatgctac tgcaggtaca tgcgaagaaa tgatcaaaag agctgtattt
5221 gctagagaat tgggcgttcc gatcgtaatg catgactact taacgggggg attcaccgca
5281 aatactagct tggctcatta ttgccgagat aatggtctac ttcttcacat ccaccgtgca
5341 atgcatgcgg ttattgatag acagaagaat catggtatcc acttccgggt attagcaaaa
5401 gcgttacgta tgtctggtgg agatcatatt cactctggta ccgtagtagg taaacttgaa
5461 ggtgaaagag acataacttt gggctttgtt gatttactgc gtgatgattt tgttgaacaa
5521 gatcgaagtc gcggtattta tttcactcaa gattgggtct ctttaccagg tgttctaccc
5581 gtggcttcag gaggtattca cgtttggcat atgcctgctc tgaccgagat ctttggggat
5641 gattccgtac tacagttcgg tggaggaact ttaggacatc cttggggtaa tgcgccaggt
5701 gccgtagcta atcgagtagc tctagaagca tgtgtaaaag ctcgtaatga aggacgtgat
5761 cttgctcagg aaggtaatga aattattcgc gaggcttgca aatggagccc ggaactagct
5821 gctgcttgtg aagtatggaa agagatcgta tttaattttg cagcagtgga cgttttggat
5881 aagtaaacta gtactaagct tgaaggtaag cctatcccta accctctcct cggtctcgat
5941 tctacgcgta ccggtcatca tcaccatcac cattgagttt aaacggtctc cagcttggct
6001 gttttggcgg atgagagaag attttcagcc tgatacagat taaatcagaa cgcagaagcg
6061 gtctgataaa acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc
6121 cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc catgcgagag
6181 tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
6241 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat
6301 ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc
6361 aggcatcaaa ttaagcagaa ggccatcctg acggatggcc ttttgccggc cacgatgcgt
6421 ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact ataggggaat
6481 tgtgagcgga taacaattcc cctctagaaa taattttgtt taactttaag aaggagatat
6541 acatatgaat gtaaaggaaa tagcttttga ccagcactct agagctgctc tacaagctgg
6601 tattgataag cttgctgatt gtgttggtct tacgcttggc cccagaggga ggaatgttgt
6661 gttggatgaa tttggaagtc ctaaggttgt gaatgatgga gtcaccattg ctagagctat
6721 tgagttacct aatgccatgg aaaatgctgg tgcagcgctt attcgtgagg ttgcgagtaa
6781 gactaatgac tctgctggtg atgggacaac cactgcgtct atccttgctc gggagataat
6841 caaacatgga ttgttgagcg tcacttctgg tgcgaatccc gtttcactca agaggggaat
6901 tgataagact gttcaaggtc tgatcgaaga gcttcagaag aaagctaggc ccgtgaaagg
6961 tcgtgatgac atcagagctg tggcttctat ctctgctgga aatgatgacc ttatagggtc
7021 aatgattgct gatgccatcg acaaagttgg acctgatggt gttttgtcca ttgaatcttc
7081 atcttctttt gagaccacgg tcgaagttga agaagggatg gagattgaca gaggttacat
7141 ctcgcctcag tttgttacaa accctgagaa actactagct gagtttgaga atgctagggt
7201 attgatcact gatcagaaga tcactgcgat caaagacatc atccctattt tggagaagac
7261 cactcagctt cgtgctccgt tgttgattat tgcagaggat gttactggtg aagccttagc
7321 tacccttgtc gtgaacaagc ttcgtggtgt cctcaatgtt gttgccgtta aagcgccagg
7381 atttggagaa agaagaaaag ccatgcttca agatattgca atcttgacag gagctgagta
7441 cctagccatg gacatgagct tactggttga aaacgcaacc atagatcaat tgggtattgc
7501 ccgaaaagtc acgattagca aagattcgac taccctcatt gcagatgcag cttccaagga
7561 cgaattacaa gctcggattg ctcagctgaa gaaagaacta tttgagactg attctgtgta
7621 tgactcagag aagctcgctg agagaattgc gaagctatct ggaggtgttg ctgtcattaa
7681 agtcggagca gcaactgaaa ctgagcttga ggaccgtaag cttcgtatcg aggatgcaaa
7741 gaacgcaaca tttgctgcta tcgaggaagg catagttcca ggtggtggtg ctgctttggt
7801 gcatctctcc actgttattc ccgccattaa ggagactttt gaggatgctg atgaacgttt
7861 gggagctgac atagtacaaa aggcattgct gtcaccagct gctcttattg ctcagaacgc
7921 tggagttgaa ggagaagttg ttgtggaaaa gattatgttc agcgattggg agaatgggta
7981 caacgcaatg actgatactt acgagaatct gttcgaagct ggagtgattg atccggctaa
8041 agtgacaaga tgtgcgctac agaacgctgc ttccgtagca ggaatggtac tgaccactca
8101 ggccattgtt gttgacaaac cgaaacccaa ggctcctgct gctgctgctc ctgagggtct
8161 catggtgtaa ggagctagaa ataattttgt ttaactttaa gaaggagata tacatatggc
8221 agcaaaggaa ttacatttca acaaagatgg gactaccatt aggagacttc aagctggtgt
8281 caacaagctc gcagatcttg ttggcgttac acttggaccc aaaggacgaa atgttgttct
8341 tgagagcaag tatggatcac cacgaattgt taatgatggt gtgactgttg caagagaggt
8401 tgagttggaa gacccggttg agaacattgg cgctaagctt gtgaggcaag cggctgccaa
8461 aaccaatgac ttggctggtg acggtacaac aacatctgtt gttcttgcac aaggttttat
8521 cgctgagggt gtcaaggtgg tagctgctgg tgcaaaccct gttttgatca ccagaggtat
8581 tgagaagaca gcaaaggctt tggttaccga gctcaagaaa atgtctaagg aggttgaaga
8641 cagtgaactt gcagatgttg cagctgttag tgcgggtaac aacgacgaaa ttggaaatat
8701 gattgctgaa gcaatgagca aagtgggcag gaaaggtgtg gttacccttg aggagggtaa
8761 aagtgccgag aacaacctct acgtcgtaga aggaatgcaa tttgaccgtg gttatatctc
8821 tccttacttt gtgacagaca gcgagaaaat gtccgtcgag ttcgacaatt gcaagttact
8881 tctcgttgac aagaaaatta ccaatgcaag ggatcttgtt ggtgttttgg aggatgcaat
8941 tagaggcgga tacccaatat taattattgc tgaagacatt gaacaagaag ctttagcgac
9001 tcttgttgta aacaagctta gaggtacact gaagatagca gctctcagag ctccagggtt
9061 tggagagcgc aagagccaat accttgatga tattgccatt ctaactggag caactgtgat
9121 ccgtgaggaa gttggtcttt cacttgacaa agctggaaaa gaggttcttg gtaatgcttc
9181 aaaggttgtc ctcacgaagg agacctcgac catagtgggt gatggaagca cacaggatgc
9241 agtgaaaaag cgtgttacac agattaaaaa tcttattgag caagcggagc aagattacga
9301 gaaggagaaa ctcaatgaga gaattgcaaa gctctctggt ggagttgctg tgattcaggt
9361 tggagcacaa actgagacag aactcaaaga gaagaagttg agagttgaag atgctcttaa
9421 tgcaacaaag gccgctgtcg aggaaggtat tgttgttggt ggtggttgca ctctgcttcg
9481 tcttgcttcc aaggttgatg ccattaaagc cacgcttgat aatgatgaag aaaaggttgg
9541 agcggatata gttaaaagag cactaagtta ccccctaaaa ctaatcgcca agaatgctgg
9601 tgttaatgga agtgtagtta gcgagaaggt gctttccaac gataacgtga aattcggtta
9661 caatgctgca accggcaagt acgaggattt gatggctgca ggaatcatcg atccaacaaa
9721 ggttgtgaga tgttgcttgg aacacgcagc ttcggttgca aagactttct tgatgtcgga
9781 ctgcgttgtt gttgagatca aggaacctga accagttccc gtcggcaacc caatggacaa
9841 ctcaggttac ggatactaag gtcgacgtcg accggataac aattgccctc tagaaataat
9901 tttgtttaac tttaagaagg agatatacat atggcctctg ttgttgcccc taagtatact
9961 tcaattaagc cattgggaga tcgagttttg gtgaagatca aggaggcaga ggagaagact
10021 ttaggtggta tcttacttcc atccactgct caatcaaaac ctcaaggagg tgaagtcgtt
10081 gctgtgggtg aaggaagaac tattgggaag aacaaaattg atatcactgt ccctactgga
10141 gcacaaatta tctactccaa atacgcagga actgaggtgg agttcaatga tgtgaagcat
10201 cttatcctca aggaagatga tattgttggc attcttgaga cagaggacat caaagatctc
10261 aaacctttga atgaccgagt ctttattaag gttgctgagg cggaggagaa aacagctgga
10321 gggttgttgt taaccgagac taccaaagag aagccttcta ttggcaccgt gatagcagtt
10381 ggaccgggtt ccctagacga ggaaggtaaa attacgcctc taccagtatc aaccggaagc
10441 acagtacttt actccaagta tgctggtaac gacttcaagg gcaaagatgg ttccaactac
10501 attgccctca gagcttcaga tgtgatggct atactttctt agttatgtta tatctttgta
10561 atctgcaaca agctgggtac cggtaccgaa aggaagctga gttggctgct gccaccgctg
10621 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
10681 aaggaggaac tatatccgga tatcccgcaa gaggcccggc agtaccggca taaccaagcc
10741 tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt
10801 catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagcta
10861 gcttatc
//
