质粒名称: | pFLP3 |
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出品公司: | 爱迪基因 |
目录编号: | 64946 |
存储实验室: | Herbert Schweizer |
载体骨架基本信息 |
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载体名称: | pFLP1 |
载体出品公司: | Herbert Schweizer |
载体抗性: | 氨苄青霉素和四环素 |
空载体大小: | 10925 bp |
完整质粒大小: | -- |
载体修饰: | -- |
载体类型: | 细菌表达 |
筛选标记: | -- |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | Flp recombinase |
物种来源: | -- |
插入大小: | -- |
基因突变: | -- |
GenBank编号 | -- |
启动子 | -- |
标签/融合蛋白: | -- |
克隆方法: | -- |
5' 克隆位点: | -- |
3' 克隆位点: | -- |
5' 测序引物 | -- |
3' 测序引物 | -- |
文献引用方法 |
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材料和方法部分: | pFLP3 was a gift from Herbert Schweizer (Addgene plasmid # 64946 ; http://n2t.net/addgene:64946 ; RRID:Addgene_64946) |
参考文献部分: | A Tn7-based broad-range bacterial cloning and expression system. Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer RR, Schweizer HP. Nat Methods. 2005 Jun;2(6):443-8. 10.1038/nmeth765 PubMed 15908923 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pFLP3 10925 bp ds-DNA circular SYN 06-AUG-2018 DEFINITION Flp recombinase expression vector. ACCESSION . VERSION . KEYWORDS pFLP3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10925) AUTHORS Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer RR, Schweizer HP TITLE A Tn7-based broad-range bacterial cloning and expression system. JOURNAL Nat Methods. 2005 Jun;2(6):443-8. PUBMED 15908923 REFERENCE 2 (bases 1 to 10925) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10925 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(29..51) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 151..170 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" rep_origin 232..583 /label=pRO1600 oriV /note="broad-host-range origin of replication from Pseudomonas aeruginosa plasmid pRO1600; requires the pRO1600 Rep protein for replication (West et al., 1994)" CDS 597..1430 /codon_start=1 /product="replication protein for the broad-host-range plasmid pRO1600 from Pseudomonas aeruginosa " /label=pRO1600 Rep /translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA" primer_bind 1576..1598 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 1590..1607 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 1591..1607 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" CDS complement(2338..3759) /codon_start=1 /gene="Bacillus subtilis sacB" /product="secreted levansucrase that renders bacterial growth sensitive to sucrose" /label=SacB /note="negative selection marker" /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV VKDSILEQGQLTVNK" promoter complement(3760..4205) /gene="sacR" /label=sacB promoter /note="sacB promoter and control region" promoter 4420..4448 /label=tet promoter /note="E. coli promoter for tetracycline efflux protein gene" CDS 4496..5686 /codon_start=1 /gene="tet" /product="tetracycline efflux protein" /label=TcR /note="confers resistance to tetracycline" /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST" primer_bind complement(4848..4867) /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" CDS complement(6054..7325) /codon_start=1 /product="site-specific recombinase" /label=FLP /note="FLP is a site-specific recombinase from Saccharomyces cerevisiae. Recombination occurs at FRT sequences." /translation="MPQFGILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR YPAWNGIISQEVLDYLSSYINRRI" RBS 7329..7337 /label=Shine-Dalgarno sequence /note="full consensus sequence for ribosome-binding sites upstream of start codons in E. coli; complementary to a region in the 3' end of the 16S rRNA (Chen et al., 1994)" CDS 7426..8139 /codon_start=1 /gene="cIts" /product="temperature-sensitive variant of the phage lambda repressor" /label=-lambda repressor (ts) /note="thermosensitivity is conferred by the A67T mutation" /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ SGVGALFNGINALNAYNAALLTKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY PVFSHVQAGMFSPKLRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV VGKVIASQWPEETFG" primer_bind complement(8428..8444) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8428..8444) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8441..8463) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8452..8468 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8476..8506) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8521..8542 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8659..8676) /label=L4440 /note="L4440 vector, forward primer" oriT 8926..9034 /note="incP origin of transfer" rep_origin complement(9106..9694) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9186..9205) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9865..10725) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10488..10507 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10726..10830) /gene="bla" /label=AmpR promoter primer_bind 10898..10916 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatacctc tcaggcgccg ctggtgccgc tggttggacg ccaagggtga atccgcctcg 241 ataccctgat tactcgcttc ctgcgccctc tcaggcggcg ataggggact ggtaaaacgg 301 ggattgccca gacgcctccc ccgccccttc aggggcacaa atgcggcccc aacggggcca 361 cgtagtggtg cgttttttgc gtttccaccc ttttcttcct tttccctttt aaacctttta 421 ggacgtctac aggccacgta atccgtggcc tgtagagttt aaaaagggac ggatttgttg 481 ccattaaggg acggatttgt tgttaagaag ggacggattt gttgttgtaa agggacggat 541 ttgttgtatt gtgggacgca gatacagtgt ccccttatac acaaggaatg tcgaacgtgg 601 cctcaccccc aatggtttac aaaagcaatg ccctggtcga ggccgcgtat cgcctcagtg 661 ttcaggaaca gcggatcgtt ctggcctgta ttagccaggt gaagaggagc gagcctgtca 721 ccgatgaagt gatgtattca gtgacggcgg aggacatagc gacgatggcg ggtgtcccta 781 tcgaatcttc ctacaaccag ctcaaagaag cggccctgcg cctgaaacgg cgggaagtcc 841 ggttaaccca agagcccaat ggcaagggga aaagaccgag tgtgatgatt accggctggg 901 tgcaaacaat catctaccgg gagggtgagg gccgtgtaga actcaggttc accaaagaca 961 tgctgccgta cctgacggaa ctcaccaaac agttcaccaa atacgccttg gctgacgtgg 1021 ccaagatgga cagcacccac gcgatcaggc tttacgagct gctcatgcaa tgggacagca 1081 tcggccagcg cgaaatagaa attgaccagc tgcgaaagtg gtttcaactg gaaggccggt 1141 atccctcgat caaggacttc aagttgcgag tgcttgatcc agccgtgacg cagatcaacg 1201 agcacagccc gctacaggtg gagtgggcgc agcgaaagac cgggcgcaag gtcacacatc 1261 tgttgttcag ttttggaccg aagaagcccg ccaaggcggt gggtaaggcc ccagcgaagc 1321 gcaaggccgg gaagatttca gatgctgaga tcgcgaaaca ggctcgccct ggtgagacat 1381 gggaagcggc ccgcgctcga ctaacccaga tgccgctgga tctggcctag aggccgtggc 1441 caccacggcc cggcctgcct ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg 1501 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg 1561 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagcttgcat 1621 gcctgcagtt cacttacacc gcttctcaac ccggtacgca ccagaaaatc attgatatgg 1681 ccatgaatgg cgttggatgc cgggcaacag cccgcattat gggcgttggc ctcaacacga 1741 ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac agccgggcag 1801 tgacgtcatc gtctgcgcgg aaatggacga acagtggggc tatgtcgggg ctaaatcgcg 1861 ccagcgctgg ctgttttacg cgtatgacag tctccggaag acggttgttg cgcacgtatt 1921 cggtgaacgc actatggcga cgctggggcg tcttatgagc ctgctgtcac cctttgacgt 1981 ggtgatatgg atgacggatg gctggccgct gtatgaatcc cgcctgaagg gaaagctgca 2041 cgtaatcagc aagcgatata cgcagcgaat tgagcggcat aacctgaatc tgaggcagca 2101 cctggcacgg ctgggacgga agtcgctgtc gttctcaaaa tcggtggagc tgcatgacaa 2161 agtcatcggg cattatctga acataaaaca ctatcaataa gttggagtca ttaccaaaag 2221 gttaggaata cggttagcca tttgcctgct tttatatagt tcatatggga ttcaccttta 2281 tgttgataag aaataaaaga aaatgccaat aggatatcgg cattttcttt tgcgttttta 2341 tttgttaact gttaattgtc cttgttcaag gatgctgtct ttgacaacag atgttttctt 2401 gcctttgatg ttcagcagga agctaggcgc aaacgttgat tgtttgtctg cgtagaatcc 2461 tctgtttgtc atatagcttg taatcacgac attgtttcct ttcgcttgag gtacagcgaa 2521 gtgtgagtaa gtaaaggtta catcgttagg atcaagatcc atttttaaca caaggccagt 2581 tttgttcagc ggcttgtatg ggccagttaa agaattagaa acataaccaa gcatgtaaat 2641 atcgttagac gtaatgccgt caatcgtcat ttttgatccg cgggagtcag tgaacagata 2701 ccatttgccg ttcattttaa agacgttcgc gcgttcaatt tcatctgtta ctgtgttaga 2761 tgcaatcagc ggtttcatca cttttttcag tgtgtaatca tcgtttagct caatcatacc 2821 gagagcgccg tttgctaact cagccgtgcg ttttttatcg ctttgcagaa gtttttgact 2881 ttcttgacgg aagaatgatg tgcttttgcc atagtatgct ttgttaaata aagattcttc 2941 gccttggtag ccatcttcag ttccagtgtt tgcttcaaat actaagtatt tgtggccttt 3001 atcttctacg tagtgaggat ctctcagcgt atggttgtcg cctgagctgt agttgccttc 3061 atcgatgaac tgctgtacat tttgatacgt ttttccgtca ccgtcaaaga ttgatttata 3121 atcctctaca ccgttgatgt tcaaagagct gtctgatgct gatacgttaa cttgtgcagt 3181 tgtcagtgtt tgtttgccgt aatgtttacc ggagaaatca gtgtagaata aacggatttt 3241 tccgtcagat gtaaatgtgg ctgaacctga ccattcttgt gtttggtctt ttaggataga 3301 atcatttgca tcgaatttgt cgctgtcttt aaagacgcgg ccagcgtttt tccagctgtc 3361 aatagaagtt tcgccgactt tttgatagaa catgtaaatc gatgtgtcat ccgcattttt 3421 aggatctccg gctaatgcaa agacgatgtg gtagccgtga tagtttgcga cagtgccgtc 3481 agcgttttgt aatggccagc tgtcccaaac gtccaggcct tttgcagaag agatattttt 3541 aattgtggac gaatcgaact caggaacttg atatttttca tttttttgct gttcagggat 3601 ttgcagcata tcatggcgtg taatatggga aatgccgtat gtttccttat atggcttttg 3661 gttcgtttct ttcgcaaacg cttgagttgc gcctcctgcc agcagtgcgg tagtaaaggt 3721 taatactgtt gcttgttttg caaacttttt gatgttcatc gttcatgtct ccttttttat 3781 gtactgtgtt agcggtctgc ttcttccagc cctcctgttt gaagatggca agttagttac 3841 gcacaataaa aaaagaccta aaatatgtaa ggggtgacgc caaagtatac actttgccct 3901 ttacacattt taggtcttgc ctgctttatc agtaacaaac ccgcgcgatt tacttttcga 3961 cctcattcta ttagactctc gtttggattg caactggtct attttcctct tttgtttgat 4021 agaaaatcat aaaaggattt gcagactacg ggcctaaaga actaaaaaat ctatctgttt 4081 cttttcattc tctgtatttt ttatagtttc tgttgcatgg gcataaagtt gcctttttaa 4141 tcacaattca gaaaatatca taatatctca tttcactaaa taatagtgaa cggcaggtat 4201 atgtgatggg ttaaaaagga tcgatcctct agagaatagg aacttcggaa taggaacttc 4261 aagatcccct gattcccttt gtcaacagca atggatactg actcgctgcg ctcggtcgtt 4321 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 4381 ggggataacg caggaaagaa catgaattaa ttctcatgtt tgacagctta tcatcgatta 4441 gctttaatgc ggtagtttat cacagttaaa ttgctaacgc agtcaggcac cgcgtatgaa 4501 atctaacaat gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg taggcatagg 4561 cttggttatg ccggtactgc cgggcctctt gcgggatatc gtccattccg acagcatcgc 4621 cagtcactat ggcgtgctgc tagcgctata tgcgttgatg caatttctat gcgcacccgt 4681 tctcggagca ctgtccgacc gctttggccg ccgcccagtc ctgctcgctt cgctacttgg 4741 agccactatc gactacgcga tcatggcgac cacacccgtc ctgtggattc tctacgccgg 4801 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga 4861 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt 4921 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tacatgcacc 4981 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca 5041 ggagtcgcat aagggagagc gccgaccgat gcccttgaga gccttcaacc cagtcagctc 5101 cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat 5161 gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg 5221 ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc 5281 tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc 5341 cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat 5401 ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc 5461 catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat cgctcgcggc 5521 tcttaccagc ctaacttcga tcactggacc gctgatcgtc acggcgattt atgccgcctc 5581 ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc ttgtctgcct 5641 ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctgaatgg aagccggcgg 5701 cacctcgcta acggattcac cactccaaga attggagcca atcaattctt gcggagaact 5761 gtgaatgcgc aaaccaaccc ttggggctgc aggaattcca ttgagtaagt ttttaagcac 5821 atcagcttca aaagcgctct gaagttccta tactttctag aggatccccc tagagaatag 5881 gaacttcgga ataggaactt caaagcgttt ccgaaaacga gcgcttccga aaatgcaacg 5941 cgagctgcgc acatacagct cactgttcac gtcgcaccta tatctgcgtg ttgcctgtat 6001 atatatatac atgagaagaa cggcatagtg cgtgtttatg cttaaatgcg tacttatatg 6061 cgtctattta tgtaggatga aaggtagtct agtacctcct gtgatattat cccattccat 6121 gcggggtatc gtatgcttcc ttcagcacta ccctttagct gttctatatg ctgccactcc 6181 tcaattggat tagtctcatc cttcaatgct atcatttcct ttgatattgg atcatatgca 6241 tagtaccgag aaactagtgc gaagtagtga tcaggtattg ctgttatctg atgagtatac 6301 gttgtcctgg ccacggcaga agcacgctta tcgctccaat ttcccacaac attagtcaac 6361 tccgttaggc ccttcattga aagaaatgag gtcatcaaat gtcttccaat gtgagatttt 6421 gggccatttt ttatagcaaa gattgaataa ggcgcatttt tcttcaaagc tttattgtac 6481 gatctgacta agttatcttt taataattgg tattcctgtt tattgcttga agaattgccg 6541 gtcctattta ctcgttttag gactggttca gaattcctca aaaattcatc caaatataca 6601 agtggatcga tcctacccct tgcgctaaag aagtatatgt gcctactaac gcttgtcttt 6661 gtctctgtca ctaaacactg gattattact cccagatact tattttggac taatttaaat 6721 gatttcggat caacgttctt aatatcgctg aatcttccac aattgatgaa agtagctagg 6781 aagaggaatt ggtataaagt ttttgttttt gtaaatctcg aagtatactc aaacgaattt 6841 agtattttct cagtgatctc ccagatgctt tcaccctcac ttagaagtgc tttaagcatt 6901 tttttactgt ggctatttcc cttatctgct tcttccgatg attcgaactg taattgcaaa 6961 ctacttacaa tatcagtgat atcagattga tgtttttgtc catagtaagg aataattgta 7021 aattcccaag caggaatcaa tttctttaat gaggcttcca gaattgttgc tttttgcgtc 7081 ttgtatttaa actggagtga tttattgaca atatcgaaac tcagcgaatt gcttatgata 7141 gtattatagc tcatgaatgt ggctctcttg attgctgttc cgttatgtgt aatcatccaa 7201 cataaatagg ttagttcagc agcacataat gctattttct cacctgaagg tctttcaaac 7261 ctttccacaa actgacgaac aagcacctta ggtggtgttt tacataatat accaaattgt 7321 ggcatacaac ctccttagta catgcaacca ttatcaccgc cagaggtaaa atagtcaaca 7381 cgcacggtgt tagatattta tcccttgcgg tgatagattt aacgtatgag cacaaaaaag 7441 aaaccattaa cacaagagca gcttgaggac gcacgtcgcc ttaaagcaat ttatgaaaaa 7501 aagaaaaatg aacttggctt atcccaggaa tctgtcgcag acaagatggg gatggggcag 7561 tcaggcgttg gtgctttatt taatggcatc aatgcattaa atgcttataa cgccgcattg 7621 cttacaaaaa ttctcaaagt tagcgttgaa gaatttagcc cttcaatcgc cagagaaatc 7681 tacgagatgt atgaagcggt tagtatgcag ccgtcactta gaagtgagta tgagtaccct 7741 gttttttctc atgttcaggc agggatgttc tcacctaagc ttagaacctt taccaaaggt 7801 gatgcggaga gatgggtaag cacaaccaaa aaagccagtg attctgcatt ctggcttgag 7861 gttgaaggta attccatgac cgcaccaaca ggctccaagc caagctttcc tgacggaatg 7921 ttaattctcg ttgaccctga gcaggctgtt gagccaggtg atttctgcat agccagactt 7981 gggggtgatg agtttacctt caagaaactg atcagggata gcggtcaggt gtttttacaa 8041 ccactaaacc cacagtaccc aatgatccca tgcaatgaga gttgttccgt tgtggggaaa 8101 gttatcgcta gtcagtggcc tgaagagacg tttggctgat cggcaaggtg ttctggtcgg 8161 cgcatagctg ataacaattg agcaagaatc ttcatcgaat taggggaatt ttcactcccc 8221 tcagaacata acatagtaaa tggattgaat tatgaagaat ggtttttatg cgacttaccg 8281 cagcaaaaat aaagggaaag ataagcgctc aataaacctg tctgttttcc ttaattctct 8341 gctggctgat aatcatcacc tgcaggttgg ctccaattat ttgtatattc ataaaatcgg 8401 ggtaccgagc tcgaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 8461 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 8521 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 8581 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 8641 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 8701 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 8761 aggaaagaac atggggattc cttaaggtat actttccgct gcataaccct gcttcggggt 8821 cattatagcg attttttcgg tatatccatc ctttttcgca cgatatacag gattttgcca 8881 aagggttcgt gtagactttc cttggtgtat ccaacggcgt cagccgggca ggataggtga 8941 agtaggccca cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc acctggcggt 9001 gctcaacggg aatcctgctc tgcgaggctg gccgataagc tagcttatgt gagcaaaagg 9061 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 9121 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 9181 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 9241 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 9301 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 9361 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 9421 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 9481 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 9541 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 9601 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 9661 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 9721 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 9781 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 9841 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 9901 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 9961 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 10021 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 10081 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 10141 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 10201 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 10261 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 10321 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 10381 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 10441 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 10501 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 10561 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 10621 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 10681 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 10741 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 10801 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 10861 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 10921 tcgtc //
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