| 质粒名称: | pGEN-luxCDABE |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 44918 |
| 存储实验室: | Harry Mobley |
|
载体骨架基本信息 |
|
| 载体名称: | pGENlux |
| 载体出品公司: | M. Chelsea Lane |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 11000 bp |
| 完整质粒大小: | 11119 bp |
| 载体类型: | 荧光素酶表达 |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | sigma 70 promoter,em7 |
| 物种来源: | synthetic construct |
| 插入大小: | 200 bp |
| 启动子 | em7 |
| 标签/融合蛋白: | lux转录融合 |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | PmeI(未破坏) |
| 3' 克隆位点: | SnaBI (未破坏) |
| 5' 测序引物 | CATATGAAGCTTGGTACCGGGATC |
| 3' 测序引物 | CTTTCGGGAAAGATTTCAACCTGG |
|
文献引用方法 |
|
| 材料和方法部分: | pGEN-luxCDABE was a gift from Harry Mobley (Addgene plasmid # 44918 ; http://n2t.net/addgene:44918 ; RRID:Addgene_44918) |
| 参考文献部分: | Expression of flagella is coincident with uropathogenic Escherichia coli ascension to the upper urinary tract. Lane MC, Alteri CJ, Smith SN, Mobley HL. Proc Natl Acad Sci U S A. 2007 Oct 16;104(42):16669-74. Epub 2007 Oct 9. 10.1073/pnas.0607898104 PubMed 17925449 |
买家导航
pGENluxCDABE载体基本信息
pGENluxCDABE载体图谱质粒图谱和多克隆位点信息
pGENluxCDABE载体简介
pGENluxCDABE载体序列
LOCUS pGEN-luxCDABE 11109 bp ds-DNA circular SYN 04-APR-2019
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pGEN-luxCDABE
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11109)
AUTHORS Lane MC, Alteri CJ, Smith SN, Mobley HL
TITLE Expression of flagella is coincident with uropathogenic Escherichia
coli ascension to the upper urinary tract.
JOURNAL Proc Natl Acad Sci U S A. 2007 Oct 16;104(42):16669-74. Epub 2007
Oct 9.
PUBMED 17925449
REFERENCE 2 (bases 1 to 11109)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 4, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11109
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 887..934
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 1021..2463
/codon_start=1
/gene="luxC"
/product="LuxC fatty acid reductase"
/label=LuxC
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
DVAVEIEQTRFLEEDKFLVFVP"
CDS 2476..3399
/codon_start=1
/gene="luxD"
/product="LuxD acyltransferase"
/label=LuxD
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
FEHLTIATVNERRMRIEIENQAISLS"
CDS 3448..4530
/codon_start=1
/gene="luxA"
/product="LuxA luciferase subunit"
/label=LuxA
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
KLFQSDVMPFLKEKQRSLLY"
CDS 4545..5528
/codon_start=1
/gene="luxB"
/product="LuxB luciferase subunit"
/label=LuxB
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
CDS 5707..6819
/codon_start=1
/gene="luxE"
/product="LuxE"
/label=LuxE
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
primer_bind 6923..6943
/label=GFP-F
/note="GFP, forward primer. Does NOT anneal to EGFP"
rep_origin complement(7074..7619)
/direction=LEFT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin.
"
primer_bind complement(7154..7173)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
terminator 7783..7869
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
primer_bind 8022..8039
/label=pBAD Reverse
/note="For vectors with E. coli araBAD promoter, reverse
primer"
CDS complement(8584..9444)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9207..9226
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9445..9549)
/gene="bla"
/label=AmpR promoter
primer_bind 9617..9635
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 taataactat ctgaagcaac gaattaatga tgagaacgaa atatcaatag tcaccgaagc
61 aatgaatgaa gcacttcgta aacttgagca acgtgtatta aatacgctca atgaattttc
121 tggttatact catgttatgg ttataggcgg tggcgcagaa ttaatatgcg atgcagtaaa
181 aaaacacaca cagattcgtg atgaacgttt tttcaaaacc aataactctc aatatgattt
241 agttaacggt atgtatctca taggtaatta atgatggaca agcgcagaac cattgccttc
301 aaactaaatc cagatgtaaa tcaaacagat aaaattgttt gtgatacact ggacagtatc
361 ccgcaagggg aacgaagccg ccttaaccgg gccgcactga cggcaggtct ggccttatac
421 agacaagatc cccggacccc tttcctttta tgtgagctgc tgacgaaaga aaccacattt
481 tcagatatcg tgaatatatt gagatcgcta tttccaaaag agatggccga ttttaattct
541 tcaatagtca ctcaatcctc ttcacaacaa gagcaaaaaa gtgatgaaga gaccaaaaaa
601 aatgcgatga agctaataaa ttaattcaat tattattgag ttccctttat ccactatcag
661 gctggataaa gggaactcaa tcaagttatt ttcttaccag tcattacata atcgttatta
721 tgaaataatc gtttgcactg tctctgttat tcaggcaatt tcaataaagg cacttgctca
781 cgctctgtca ttttctgaaa ctctcatgtg gaattcctgc agggcatgcc ccgggcatat
841 gaagcttggt accgggatcc gcggccgcgt ttaaacccat ggacgtgttg acaattaatc
901 atcggcatag tatatcggca tagtataata cgactcaggg cccactagtg gtacccgggg
961 atcctctaga gtcgacctgc aggtcgacgg atccggggaa ttcaggcttg gaggatacgt
1021 atgactaaaa aaatttcatt cattattaac ggccaggttg aaatctttcc cgaaggtgat
1081 gatttagtgc aatccattaa ttttggtgat aatagtgttt acctgccaat attgaatgac
1141 tctcatgtaa aaaacattat tgattgtaat ggaaataacg aattacggtt gcataacatt
1201 gtcaattttc tctatacggt agggcaaaga tggaaaaatg aagaatactc aagacgcagg
1261 acatacattc gtgacttaaa aaaatatatg ggatattcag aagaaatggc taagctagag
1321 gccaattgga tatctatgat tttatgttct aaaggcggcc tttatgatgt tgtagaaaat
1381 gaacttggtt ctcgccatat catggatgaa tggctacctc aggatgaaag ttatgttcgg
1441 gcttttccga aaggtaaatc tgtacatctg ttggcaggta atgttccatt atctgggatc
1501 atgtctatat tacgcgcaat tttaactaag aatcagtgta ttataaaaac atcgtcaacc
1561 gatcctttta ccgctaatgc attagcgtta agttttattg atgtagaccc taatcatccg
1621 ataacgcgct ctttatctgt tatatattgg ccccaccaag gtgatacatc actcgcaaaa
1681 gaaattatgc aacatgcgga tgttattgtc gcttggggag ggccagatgc gattaattgg
1741 gcggtagagc atgcgccatc ttatgctgat gtgattaaat ttggttctaa aaagagtctt
1801 tgcattatcg ataatcctgt tgatttgacg tccgcagcga caggtgcggc tcatgatgtt
1861 tgtttttacg atcagcgagc ttgtttttct gcccaaaaca tatattacat gggaaatcat
1921 tatgaggaat ttaagttagc gttgatagaa aaacttaatc tatatgcgca tatattaccg
1981 aatgccaaaa aagattttga tgaaaaggcg gcctattctt tagttcaaaa agaaagcttg
2041 tttgctggat taaaagtaga ggtggatatt catcaacgtt ggatgattat tgagtcaaat
2101 gcaggtgtgg aatttaatca accacttggc agatgtgtgt accttcatca cgtcgataat
2161 attgagcaaa tattgcctta tgttcaaaaa aataagacgc aaaccatatc tatttttcct
2221 tgggagtcat catttaaata tcgagatgcg ttagcattaa aaggtgcgga aaggattgta
2281 gaagcaggaa tgaataacat atttcgagtt ggtggatctc atgacggaat gagaccgttg
2341 caacgattag tgacatatat ttctcatgaa aggccatcta actatacggc taaggatgtt
2401 gcggttgaaa tagaacagac tcgattcctg gaagaagata agttccttgt atttgtccca
2461 taataggtaa aaagtatgga aaatgaatca aaatataaaa ccatcgacca cgttatttgt
2521 gttgaaggaa ataaaaaaat tcatgtttgg gaaacgctgc cagaagaaaa cagcccaaag
2581 agaaagaatg ccattattat tgcgtctggt tttgcccgca ggatggatca ttttgctggt
2641 ctggcggaat atttatcgcg gaatggattt catgtgatcc gctatgattc gcttcaccac
2701 gttggattga gttcagggac aattgatgaa tttacaatgt ctataggaaa gcagagcttg
2761 ttagcagtgg ttgattggtt aactacacga aaaataaata acttcggtat gttggcttca
2821 agcttatctg cgcggatagc ttatgcaagc ctatctgaaa tcaatgcttc gtttttaatc
2881 accgcagtcg gtgttgttaa cttaagatat tctcttgaaa gagctttagg gtttgattat
2941 ctcagtctac ccattaatga attgccgaat aatctagatt ttgaaggcca taaattgggt
3001 gctgaagtct ttgcgagaga ttgtcttgat tttggttggg aagatttagc ttctacaatt
3061 aataacatga tgtatcttga tataccgttt attgctttta ctgcaaataa cgataattgg
3121 gtcaagcaag atgaagttat cacattgtta tcaaatattc gtagtaatcg atgcaagata
3181 tattctttgt taggaagttc gcatgacttg agtgaaaatt tagtggtcct gcgcaatttt
3241 tatcaatcgg ttacgaaagc cgctatcgcg atggataatg atcatctgga tattgatgtt
3301 gatattactg aaccgtcatt tgaacattta actattgcga cagtcaatga acgccgaatg
3361 agaattgaga ttgaaaatca agcaatttct ctgtcttaaa atctattgag atattctatc
3421 actcaaatag caatataagg actctctatg aaatttggaa actttttgct tacataccaa
3481 cctccccaat tttctcaaac agaggtaatg aaacgtttgg ttaaattagg tcgcatctct
3541 gaggagtgtg gttttgatac cgtatggtta ctggagcatc atttcacgga gtttggtttg
3601 cttggtaacc cttatgtcgc tgctgcatat ttacttggcg cgactaaaaa attgaatgta
3661 ggaactgccg ctattgttct tcccacagcc catccagtac gccaacttga agatgtgaat
3721 ttattggatc aaatgtcaaa aggacgattt cggtttggta tttgccgagg gctttacaac
3781 aaggactttc gcgtattcgg cacagatatg aataacagtc gcgccttagc ggaatgctgg
3841 tacgggctga taaagaatgg catgacagag ggatatatgg aagctgataa tgaacatatc
3901 aagttccata aggtaaaagt aaaccccgcg gcgtatagca gaggtggcgc accggtttat
3961 gtggtggctg aatcagcttc gacgactgag tgggctgctc aatttggcct accgatgata
4021 ttaagttgga ttataaatac taacgaaaag aaagcacaac ttgagcttta taatgaagtg
4081 gctcaagaat atgggcacga tattcataat atcgaccatt gcttatcata tataacatct
4141 gtagatcatg actcaattaa agcgaaagag atttgccgga aatttctggg gcattggtat
4201 gattcttatg tgaatgctac gactattttt gatgattcag accaaacaag aggttatgat
4261 ttcaataaag ggcagtggcg tgactttgta ttaaaaggac ataaagatac taatcgccgt
4321 attgattaca gttacgaaat caatcccgtg ggaacgccgc aggaatgtat tgacataatt
4381 caaaaagaca ttgatgctac aggaatatca aatatttgtt gtggatttga agctaatgga
4441 acagtagacg aaattattgc ttccatgaag ctcttccagt ctgatgtcat gccatttctt
4501 aaagaaaaac aacgttcgct attatattag ctaaggagaa agaaatgaaa tttggattgt
4561 tcttccttaa cttcatcaat tcaacaactg ttcaagaaca aagtatagtt cgcatgcagg
4621 aaataacgga gtatgttgat aagttgaatt ttgaacagat tttagtgtat gaaaatcatt
4681 tttcagataa tggtgttgtc ggcgctcctc tgactgtttc tggttttctg ctcggtttaa
4741 cagagaaaat taaaattggt tcattaaatc acatcattac aactcatcat cctgtccgca
4801 tagcggagga agcttgctta ttggatcagt taagtgaagg gagatttatt ttagggttta
4861 gtgattgcga aaaaaaagat gaaatgcatt tttttaatcg cccggttgaa tatcaacagc
4921 aactatttga agagtgttat gaaatcatta acgatgcttt aacaacaggc tattgtaatc
4981 cagataacga tttttatagc ttccctaaaa tatctgtaaa tccccatgct tatacgccag
5041 gcggacctcg gaaatatgta acagcaacca gtcatcatat tgttgagtgg gcggccaaaa
5101 aaggtattcc tctcatcttt aagtgggatg attctaatga tgttagatat gaatatgctg
5161 aaagatataa agccgttgcg gataaatatg acgttgacct atcagagata gaccatcagt
5221 taatgatatt agttaactat aacgaagata gtaataaagc taaacaagag acgcgtgcat
5281 ttattagtga ttatgttctt gaaatgcacc ctaatgaaaa tttcgaaaat aaacttgaag
5341 aaataattgc agaaaacgct gtcggaaatt atacggagtg tataactgcg gctaagttgg
5401 caattgaaaa gtgtggtgcg aaaagtgtat tgctgtcctt tgaaccaatg aatgatttga
5461 tgagccaaaa aaatgtaatc aatattgttg atgataatat taagaagtac cacatggaat
5521 atacctaata gatttcgagt tgcagcgagg cggcaagtga acgaatcccc aggagcatag
5581 ataactatgt gactggggtg agtgaaagca gccaacaaag cagcagcttg aaagatgaag
5641 ggtataaaag agtatgacag cagtgctgcc atactttcta atattatctt gaggagtaaa
5701 acaggtatga cttcatatgt tgataaacaa gaaattacag caagctcaga aattgatgat
5761 ttgatttttt cgagcgatcc attagtgtgg tcttacgacg agcaggaaaa aatcagaaag
5821 aaacttgtgc ttgatgcatt tcgtaatcat tataaacatt gtcgagaata tcgtcactac
5881 tgtcaggcac acaaagtaga tgacaatatt acggaaattg atgacatacc tgtattccca
5941 acatcggttt ttaagtttac tcgcttatta acttctcagg aaaacgagat tgaaagttgg
6001 tttaccagta gcggcacgaa tggtttaaaa agtcaggtgg cgcgtgacag attaagtatt
6061 gagagactct taggctctgt gagttatggc atgaaatatg ttggtagttg gtttgatcat
6121 caaatagaat tagtcaattt gggaccagat agatttaatg ctcataatat ttggtttaaa
6181 tatgttatga gtttggtgga attgttatat cctacgacat ttaccgtaac agaagaacga
6241 atagattttg ttaaaacatt gaatagtctt gaacgaataa aaaatcaagg gaaagatctt
6301 tgtcttattg gttcgccata ctttatttat ttactctgcc attatatgaa agataaaaaa
6361 atctcatttt ctggagataa aagcctttat atcataaccg gaggcggctg gaaaagttac
6421 gaaaaagaat ctctgaaacg tgatgatttc aatcatcttt tatttgatac tttcaatctc
6481 agtgatatta gtcagatccg agatatattt aatcaagttg aactcaacac ttgtttcttt
6541 gaggatgaaa tgcagcgtaa acatgttccg ccgtgggtat atgcgcgagc gcttgatcct
6601 gaaacgttga aacctgtacc tgatggaacg ccggggttga tgagttatat ggatgcgtca
6661 gcaaccagtt atccagcatt tattgttacc gatgatgtcg ggataattag cagagaatat
6721 ggtaagtatc ccggcgtgct cgttgaaatt ttacgtcgcg tcaatacgag gacgcagaaa
6781 gggtgtgctt taagcttaac cgaagcgttt gatagttgat gacctgcagg catgcaagct
6841 tgcggccgcg ggccccatgg atcgatagct ggtcgacaca atctgccctt tcgaaagatc
6901 ccaacgaaaa gcgtgaccac atggtccttc ttgagtttgt aactgctgct gggattacac
6961 atggcatgga tgagctctac aaataatgag ctagcccgcc taatgagcgg gctttttttt
7021 ctcggcctag gagatactta acagggaagt gagagggccg cggcaaagcc gtttttccat
7081 aggctccgcc cccctgacaa gcatcacgaa atctgacgct caaatcagtg gtggcgaaac
7141 ccgacaggac tataaagata ccaggcgttt ccccctggcg gctcccccgt gcgctctcct
7201 gttcctgcct ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc
7261 acgcctgaca ctcagttccg ggtaggcagt tcgctccaag ctggactgta tgcacgaacc
7321 ccccgttcag tccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccgga
7381 aagacatgca aaagcaccac tggcagcagc cactggtaat tgatttagag gagttagtct
7441 tgaagccatg cgccggttaa ggctaaactg aaaggacaag ttttggtgac tgcgctcctc
7501 caagccagtt acctcggttc aaagagttgg tagctcagag aaccttcgaa aaaccgccct
7561 gcaaggcggt tttttcgttt tcagagcaag agattacgcg cagaccaaaa cgatctcaag
7621 aagatcatct tattaatcag ataaaatatt tctaggatct aaaacactag gccccgtcag
7681 gatggccttc tgcttaattt gatgcctggc agtttatggc gggcgtcctg cccgccaccc
7741 tccgggccgt tgcttcgcaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag
7801 agcgttcacc gacaaacaac agataaaacg aaaggcccag tctttcgact gagcctttcg
7861 ttttatttga tgcctggcag ttccctactc tcgcatgggg agaccccaca ctaccatcgg
7921 cgctacggcg tttcacttct gagttcggca tggggtcagg tgggaccacc gcgctactgc
7981 cgccaggcaa attctgtttt atcagaccgc ttctgcgttc tgatttaatc tgtatcaggc
8041 tgaaaatctt ctctcatccg ccaaaacagc caagctggat ctggcaaatc gctgaatatt
8101 ccttttgtct ccgaccatca ggcacctgag tcgctgtctt tttcgtgaca ttcagttcgc
8161 tgcgctcacg gctctggcag tgaatggggg taaatggcac tacaggcgcc ttttatggat
8221 tcatgcaagg aaactaccca taatacaaga aaagcccgtc acgggcttct cagggcgttt
8281 tatggcgggt ctgctatgtg gtgctatctg actttttgct gttcagcagt tcctgccctc
8341 tgattttcca gtctgaccac ttcggattat cccgtgacag gtcattcaga ctggctaatg
8401 cacccagtaa ggcagcggta tcatcaacag gcttacccgt cttactgtca accggatcta
8461 aaacactagt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
8521 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
8581 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
8641 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
8701 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
8761 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
8821 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
8881 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
8941 attcagctcc ggttcccaac gatcaaggcg agttacatgg tcccccatgt tgtgcaaaaa
9001 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
9061 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
9121 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
9181 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
9241 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
9301 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
9361 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
9421 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
9481 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
9541 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
9601 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttctag acaacatcag
9661 caaggagaaa ggggctaccg gcgaaccagc agccccttta taaaggcgct tcagtagtca
9721 gaccagcatc agtcctgaaa aggcgggcct gcgcccgcct ccaggttgct acttaccgga
9781 ttcgtaagcc atgaaagccg ccacctccct gtgtccgtct ctgtaacgaa tctcgcacag
9841 cgattttcgt gtcagataag tgaatatcaa cagtgtgaga cacacgatca acacacacca
9901 gacaagggaa cttcgtggta gtttcatggc cttcttctcc ttgcgcaaag cgcggtaaga
9961 ggctatcctg atgtggacta gacataggga tgcctcgtgg tggttaatga aaattaactt
10021 actacggggc tatcttcttt ctgccacaca acacggcaac aaaccacctt cacgtcatga
10081 ggcagaaagc ctcaagcgcc gggcacatca tagcccatat acctgcacgc tgaccacact
10141 cactttccct gaaaataatc cgctcattca gaccgttcac gggaaatccg tgtgattgtt
10201 gccgcatcac gctgcctccc ggagtttgtc tcgagcactt ttgttacccg ccaaacaaaa
10261 cccaaaaaca actcataccc aacccaataa aacaccaaaa caagacaaat aatcattgat
10321 tgatggttga aatggggtaa acttgacaaa caaacccact taaaacccaa aacataccca
10381 aacacacacc aaaaaaacac cataaggagt tttataaatg ttggtattca ttgatgacgg
10441 ttcaacaaac atcaaactac agtagcagga aagcgacgga acaattaaac agcacattag
10501 cccgaacagc ttcaaacgcg agtgggcagt ctcttttggt gataaaaagg tctttaacta
10561 cacactgaac ggcgaacagt attcatttga tccaatcagc ccggatgctg tagtcacaac
10621 caatatcgca tggcaataca gcgacgttaa tgtcgttgca gtgcatcacg ccttactgac
10681 cagtggtctg ccggtaagcg aagtggatat tgtttgcaca cttcctctga cagagtatta
10741 cgacagaaat aaccaaccca atacggaaaa tattgagcgt aagaaagcaa acttccggaa
10801 aaaaattaca ttaaatggcg gggatacatt cacaataaaa gatgtaaaag tcatgcctga
10861 atctataccg gcaggttatg aagttctaca agaactggat gagttagatt ctttattaat
10921 tatagatctc gggggcacca cattagatat ttctcaggta atggggaaat tatcggggat
10981 cagtaaaata tacggagact catctcttgg tgtctctctg gttacatctg cagtaaaaga
11041 tgccctttct cttgcgagaa caaaaggaag tagctatctt gctgacgata taatcattca
11101 cagaaaaga
//
pGENluxCDABE载体图谱质粒图谱pdf版和相关资料下载
pGENluxCDABE载体质粒应用举例
- 上一篇：pGL3 3 kb prom. CD274
- 下一篇：Nme2sgRNA_pLKO
