| 质粒名称: | pIMAY* |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 121441 |
| 存储实验室: | Tim Foster |
|
载体骨架基本信息 |
|
| 载体名称: | N/A |
| 完整质粒大小: | 5734 bp |
| 载体类型: | 葡萄球菌等位基因交换载体 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 克隆方法: | 限制性内切酶 |
| 5’测序引物 | T7 |
| 3’测序引物 | T3 |
|
文献引用方法 |
|
| 材料和方法部分: | pIMAY was a gift from Tim Foster (Addgene plasmid # 68939 ; http://n2t.net/addgene:68939 ; RRID:Addgene_68939) |
| 参考文献部分: | Transforming the untransformable: application of direct transformation to manipulate genetically Staphylococcus aureus and Staphylococcus epidermidis. Monk IR, Shah IM, Xu M, Tan MW, Foster TJ. MBio. 2012 Mar 20;3(2). pii: e00277-11. doi: 10.1128/mBio.00277-11. Print 2012. 10.1128/mBio.00277-11 PubMed 22434850 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pIMAY* 5536 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Allelic exchange plasmid with PheS* counter-selection for
Staphylococcus aureus.
ACCESSION .
VERSION .
KEYWORDS pIMAY*
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5536)
AUTHORS Schuster CF, Howard SA, Grundling A
TITLE Use of the counter selectable marker PheS* for genome engineering in
Staphylococcus aureus.
JOURNAL Microbiology. 2019 Apr 3. doi: 10.1099/mic.0.000791.
PUBMED 30942689
REFERENCE 2 (bases 1 to 5536)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5536
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 2388..2399
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
protein_bind 2594..2611
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O1 for the tetR and tetA genes"
oriT 3949..4058
/note="incP origin of transfer"
rep_origin 4200..4745
/direction=RIGHT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin.
"
primer_bind 4646..4665
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 4862..4879
/label=L4440
/note="L4440 vector, forward primer"
primer_bind 5252..5271
/label=T7
/note="T7 promoter, forward primer"
promoter 5252..5270
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 5279..5386
/label=MCS
/note="pBluescript multiple cloning site"
primer_bind 5303..5319
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 5303..5319
/label=pBluescriptSK
/note="For pBluescript vector"
primer_bind complement(5353..5369)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5354..5370)
/label=pBluescriptKS
/note="For pBluescript vector"
promoter complement(5399..5417)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
1 atattataat tatcaaagag aggggtggca aacggtattt ggcattatta ggttaaaaaa
61 tgtagaagga gagtgaaacc catgaacttt aataaaattg atttagacaa ttggaagaga
121 aaagagatat ttaatcatta tttgaaccaa caaacgactt ttagtataac cacagaaatt
181 gatattagtg ttttataccg aaacataaaa caagaaggat ataaatttta ccctgcattt
241 attttcttag tgacaagggt gataaactca aatacagctt ttagaactgg ttacaatagc
301 gacggagagt taggttattg ggataagtta gagccacttt atacaatttt tgatggtgta
361 tctaaaacat tctctggtat ttggactcct gtaaagaatg acttcaaaga gttttatgat
421 ttataccttt ctgatgtaga gaaatataat ggttcgggga aattgtttcc caaaacacct
481 atacctgaaa atgctttttc tctttctatt attccatgga cttcatttac tgggtttaac
541 ttaaatatca ataataatag taattacctt ctacccatta ttacagcagg aaaattcatt
601 aataaaggta attcaatata tttaccgcta tctttacagg tacatcattc tgtttgtgat
661 ggttatcatg caggattgtt tatgaactct attcaggaat tgtcagatag gcctaatgac
721 tggcttttat aatatgagat aatgccgact gtacttttta cagtcggttt tctaatgtca
781 ctaacctgcc ccgttagttg acatatgatc atcataattc tgtctcatta tataacatcc
841 tccatacctt ctattataga atgtattgct attaatcgca acatcaaacc aaaataaaaa
901 cccccttcga ctttcgtcag ggggctttta tttattcaat aatccctcct ctcaataaat
961 ctattgttgt acttaattca acttccattt ctctgtatct ttcaatacgc tcttttaagt
1021 ccttaatttc tttttttaat tcctcatttt cagcaaataa ctctttttct ttgtttgtca
1081 ttttatttcc cccgtttcag catcaagaac ctttgcataa cttgctctat atccacactg
1141 ataattgccc tcaaaccata atctaaaggc gctagagttt gttgaaacaa tatcttttac
1201 atcattcgta tttaaaattc caaactccgc tcccctaagg cgaataaaag ccattaaatc
1261 ttttgtattt accaaattat agtcatccac tatatctaaa agtaaattct tcaattctct
1321 tttttggctt tcatcaagtg ttatatagcg gtcaatatca aaatcattaa tgttcaaaat
1381 atcttttttg tcgtatatat gtttattctt agcaatagcg tcctttgatt catgagtcaa
1441 atattcatat gaacctttga tataatcaag tatctcaaca tgagcaactg aactattccc
1501 caattttcgc ttaatcttgt tcctaacgct ttctattgtt acaggatttc gtgcaatata
1561 tataacgtga tagtgtggtt ttttatagtg ctttccattt tgtataatat tactattatt
1621 ccatgtatct ttatcttttt tttcgtccat atcgtgtaaa ggactgacag ccatagatac
1681 gcccaaactc tctaattttt ctttccaatc attaggaatt gagtcaggat ataataaaaa
1741 tccaaaattt ctagctttag tatttttaat agccatgata taattacctt atcaaaaaca
1801 agtagcgaaa actcgtatcc ttctaaaaac gcgagctttc gcttattttt tttgttctga
1861 ttcctttctt gcatattctt ctatagctaa cgccgcaacc gcagattttg aaaaaccttt
1921 ttgtttcgcc atatctgtta attttttatc ttgctctttt gtcagagaaa tcataactct
1981 ttttttcgat tctgaaatca ccatttaaaa aactccaatc aaataatttt ataaagttag
2041 tgtatcactt tgtaatcata aaaacaacaa taaagctact taaatataga tttataaaaa
2101 acgttggcga aaacgttggc gattcgttgg cgattgaaaa accccttaaa cccttgagcc
2161 agttgggata gagcgttttt ggcacaaaaa ttggcactcg gcacttaatg gggggtcgta
2221 gtacggaagc aaaattcgct tcctttcccc ccattttttt ccaaattcca aatttttttc
2281 aaaaattttc cagcgctacc gctcggcaaa attgcaagca atttttaaaa tcaaacccat
2341 gagggaattt cattccctca tactcccttg agcctcctcc aaccgaaata gaagggcgct
2401 gcgcttatta tttcattcag tcatcggctt tcataatcta acagacaaca tcttcgctgc
2461 aaagccacgc tacgctcaag ggcttttacg ctacgataac gcctgtttta acgattatgc
2521 cgataactaa acgaaataaa cgctaaaacg catgcggatc ccgagttcat gaaaaactaa
2581 aaaaaatatt gacactctat cattgataga gcttaattaa aataagcttg atatcgaatt
2641 aaggaggaaa aaaaatgtca gaacaacaaa caatgtctga attaaaacag caagcattag
2701 tagatataaa cgaagcaaat gacgaaagag ctttacaaga agtgaaagtc aaatatttgg
2761 gaaagaaagg ttctgttagt ggattaatga aattgatgaa agacttacca aatgaagata
2821 aaccagcttt tggtcagaaa gttaatgaac taagacaaac tatacagaat gaacttgatg
2881 agagacaaca aatgctagtg aaagaaaaat taaacaaaca gctagcagaa gaaactattg
2941 atgtctcatt accaggaaga catattgaaa tcggatctaa acatccttta acgcgaacga
3001 ttgaagaaat cgaagactta tttttaggtc ttggttatga aatagtaaac ggatatgagg
3061 ttgaacaaga tcattacaac ttcgagatgt taaatcttcc aaaatctcat ccagcacgag
3121 atatgcaaga tagtttctac ataacagatg aaattttatt acgaacgcat acttctccag
3181 tacaagctag aactatggaa tcaagacatg gacaaggtcc tgtaaaaatt atttgtccag
3241 gtaaagttta cagaagagat tctgatgatg cgactcattc acatcaattt acacaaatag
3301 aaggattagt tgttgataaa aatgtgaaga tgagtgatct taaaggaaca ttggagttac
3361 ttgcaaaaaa actatttgga gctgatcgag aaataagatt aagacctagt tattttccat
3421 ttacagaacc atctgtagaa gtagatgtat cttgttttaa atgtaaaggc aaaggttgca
3481 atgtttgtaa acacacggga tggattgaaa tcttaggtgc aggcatggtt cacccaaatg
3541 tcttagagat ggctggtttt gactcaagtg agtatagtgg atttggtttt ggcatgggtc
3601 cggatagaat tgctatgtta aaatacggaa tcgaagatat tcgacacttc tacactaatg
3661 atgtacgttt tttagatcaa tttaaagcag ttgaagatcg tggagatatg taaggtacca
3721 gatctcctct cgcctgtccc ctcagttcag taatttcctg catttgcctg tttccagtcg
3781 gtagatattc cacaaaacag cagggaagca gcgcttttcc gctgcataac cctgcttcgg
3841 ggtcattata gcgatttttt cggtatatcc atcctttttc gcacgatata caggattttg
3901 ccaaagggtt cgtgtagact ttccttggtg tatccaacgg cgtcagccgg gcaggatagg
3961 tgaagtaggc ccacccgcga gcgggtgttc cttcttcact gtcccttatt cgcacctggc
4021 ggtgctcaac gggaatcctg ctctgcgagg ctggccggct accgccggcg taacagatga
4081 gggcaagcgg cggagaatta caacttatat cgtatggggc tgacttcagg tgctacattt
4141 gaagagataa attgcactga aatctagaaa tattttatct gattaataag atgatcttct
4201 tgagatcgtt ttggtctgcg cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag
4261 ggcggttttt cgaaggttct ctgagctacc aactctttga accgaggtaa ctggcttgga
4321 ggagcgcagt caccaaaact tgtcctttca gtttagcctt aaccggcgca tgacttcaag
4381 actaactcct ctaaatcaat taccagtggc tgctgccagt ggtgcttttg catgtctttc
4441 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcggact gaacgggggg
4501 ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg
4561 aatgagacaa acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag
4621 gagagcgcac gagggagccg ccagggggaa acgcctggta tctttatagt cctgtcgggt
4681 ttcgccacca ctgatttgag cgtcagattt cgtgatgctt gtcagggggg cggagcctat
4741 ggaaaaacgg ctttgccgcg gccctctcac ttccctgtta agtatcttcc tggcatcttc
4801 caggaaatct ccgccccgtt cgtaagccat ttccgctcgc cgcagtcgaa cgaccgagcg
4861 tagcgagtca gtgagcgagg aagcggaata tatcctgtat cacatattct gctgacgcac
4921 cggtgcagcc ttttttctcc tgccacatga agcacttcac tgacaccctc atcagtgcca
4981 acatagtaag ccagtataca ctccgctagc gctgatgtcc ggcggtgctt ttgccgttac
5041 gcaccacccc gtcagtagct gaacaggagg gacagctgat agaaacagaa gccactggag
5101 cacctcaaaa acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt
5161 tgcgccgaat aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc
5221 cctgttgata ccgggaagcc ctgggacgtc gtaatacgac tcactatagg gcgaattgga
5281 gctccaccgc ggtggcggcc gctctagaac tagtggatcc cccgggctgc aggaattcga
5341 tatcaagctt atcgataccg tcgacctcga gggggggccc ggtacccagc ttttgttccc
5401 tttagtgagg gttaattatc ccattatgct ttggcagttt attcttgaca tgtagtgagg
5461 gggctggtat aatcacatac ggccgataaa gcaagcatat aatattgcgt ttcatcttta
5521 gaagcgaatt tcgcca
//
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