| 质粒名称: | pLVX-IRES-Gαi*-BERKY3 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 158424 |
| 存储实验室: | Mikel Garcia-Marcos |
|
载体骨架基本信息 |
|
| 载体名称: | pLVX-IRES-Hyg |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 8519 bp |
| 完整质粒大小: | 11708 bp |
| 载体修饰: | -- |
| 载体类型: | 慢病毒载体 |
| 筛选标记: | 潮霉素 |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Lyn11-Nluc-ER/K linker-3xYFP-KB1753-myc,Gαi*-BERKY3 |
| 物种来源: | -- |
| 插入大小: | 3189 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | myc（插入件上的C端子） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | XhoI (未破坏) |
| 3' 克隆位点: | XhoI (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pLVX-IRES-Gαi*-BERKY3 was a gift from Mikel Garcia-Marcos (Addgene plasmid # 158424 ; http://n2t.net/addgene:158424 ; RRID:Addgene_158424) |
| 参考文献部分: | Revealing the Activity of Trimeric G-proteins in Live Cells with a Versatile Biosensor Design. Maziarz M, Park JC, Leyme A, Marivin A, Garcia-Lopez A, Patel PP, Garcia-Marcos M. Cell. 2020 Jun 27. pii: S0092-8674(20)30752-2. doi: 10.1016/j.cell.2020.06.020. 10.1016/j.cell.2020.06.020 PubMed 32634377 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVX-IRES-G-alpha-i*-BERKY3 11726 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION BRET biosensor for detection of G-alpha-i-GTP in lentiviral vector
pLVX-IRES-Hyg.
ACCESSION .
VERSION .
KEYWORDS pLVX-IRES-G-alpha-i*-BERKY3
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11726)
AUTHORS Maziarz M, Park JC, Leyme A, Marivin A, Garcia-Lopez A, Patel PP,
Garcia-Marcos M
TITLE Revealing the Activity of Trimeric G-proteins in Live Cells with a
Versatile Biosensor Design.
JOURNAL Cell. 2020 Jun 27. pii: S0092-8674(20)30752-2. doi:
10.1016/j.cell.2020.06.020.
PUBMED 32634377
REFERENCE 2 (bases 1 to 11726)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11726
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 1..304
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 305..508
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 459..479
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 505..529
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 696..1208
/codon_start=1
/product="NanoLuc(R) luciferase"
/label=Nluc
/note="human codon-optimized"
/translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
RLCERILA"
CDS 1233..1253
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/label=TEV site
/translation="ENLYFQG"
CDS 1974..2225
/codon_start=1
/product="C-terminal fragment of mVenus for use in
bimolecular fluorescence complementation (BiFC) (Kodama and
Hu, 2010)"
/label=VC155
/translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
CDS 2706..2957
/codon_start=1
/product="C-terminal fragment of mVenus for use in
bimolecular fluorescence complementation (BiFC) (Kodama and
Hu, 2010)"
/label=VC155
/translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
CDS 3435..3686
/codon_start=1
/product="C-terminal fragment of mVenus for use in
bimolecular fluorescence complementation (BiFC) (Kodama and
Hu, 2010)"
/label=VC155
/translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
CDS 3777..3806
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
misc_feature 3850..4423
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(4017..4034)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 4244..4263
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 4462..5481
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
misc_feature 5495..6083
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5548..5568)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5966..5977)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 6290..6923
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
primer_bind complement(7051..7067)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7051..7067)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7064..7086)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7075..7091
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7099..7129)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7144..7165
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7282..7299)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7453..8041)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7533..7552)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8212..9072)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8835..8854
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9073..9177)
/gene="bla"
/label=AmpR promoter
polyA_signal 9225..9359
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(9262..9281)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 9316..9335
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 9527..10160
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 10207..10332
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 10829..11062
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 11247..11291
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 11554..11669
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
ORIGIN
1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
541 ccatccacgc tgttttgacc tccatagaag acaccgactc tactagagga tctatttccg
601 gtgaattcct cgaggctagc atgggatgta taaaatcaaa agggaaagac agcaagcttg
661 gatcaggcgg tagcggggga tcaggcggta gcgggatggt cttcacactc gaagatttcg
721 ttggggactg gcgacagaca gccggctaca acctggacca agtccttgaa cagggaggtg
781 tgtccagttt gtttcagaat ctcggggtgt ccgtaactcc gatccaaagg attgtcctga
841 gcggtgaaaa tgggctgaag atcgacatcc atgtcatcat cccgtatgaa ggtctgagcg
901 gcgaccaaat gggccagatc gaaaaaattt ttaaggtggt gtaccctgtg gatgatcatc
961 actttaaggt gatcctgcac tatggcacac tggtaatcga cggggttacg ccgaacatga
1021 tcgactattt cggacggccg tatgaaggca tcgccgtgtt cgacggcaaa aagatcactg
1081 taacagggac cctgtggaac ggcaacaaaa ttatcgacga gcgcctgatc aaccccgacg
1141 gctccctgct gttccgagta accatcaacg gagtgaccgg ctggcggctg tgcgaacgca
1201 ttctggcgga attcggatct ggtggttcag gcgaaaacct gtattttcag ggcggaagcg
1261 gagaagagga ggaaaagaaa aaacaacagg aggaggaggc ggagaggctg aggaggatac
1321 aagaggaaat ggaaaaagaa agaaagcggc gagaggaaga tgagcagcga cgccgcaagg
1381 aggaggaaga gagaaggatg aaattggaga tggaggcaaa acgcaaacaa gaagaggaag
1441 aacggaagaa acgggaggat gatgaaaagc ggaagaaaaa gggatctgga gggtcagggg
1501 gcgcgcccat ggtttcaaag ggagaagagt tgttcacagg ggttgtaccg attcttgtag
1561 aacttgacgg agatgtcaac gggcacaagt ttagtgtaag tggagaaggc gagggcgatg
1621 cgacctatgg aaaactgacc cttaaattta tttgtactac gggtaaattg cccgttccct
1681 ggccgacatt ggtaactaca ttcggatatg gtttgatgtg ctttgcaagg tatccggatc
1741 acatgaagca gcatgatttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
1801 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
1861 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
1921 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
1981 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
2041 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
2101 acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
2161 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
2221 acaagggcgc gcccggagga atggtttcaa agggagaaga gttgttcaca ggggttgtac
2281 cgattcttgt agaacttgac ggagatgtca acgggcacaa gtttagtgta agtggagaag
2341 gcgagggcga tgcgacctat ggaaaactga cccttaaatt tatttgtact acgggtaaat
2401 tgcccgttcc ctggccgaca ttggtaacta cattcggata tggtttgatg tgctttgcaa
2461 ggtatccgga tcacatgaag cagcatgatt tcttcaagtc cgccatgccc gaaggctacg
2521 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
2581 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
2641 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
2701 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
2761 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
2821 tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg
2881 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
2941 tggacgagct gtacaaggga tccggaggaa tggtttcaaa gggagaagag ttgttcacag
3001 gggttgtacc gattcttgta gaacttgacg gagatgtcaa cgggcacaag tttagtgtaa
3061 gtggagaagg cgagggcgat gcgacctatg gaaaactgac ccttaaattt atttgtacta
3121 cgggtaaatt gcccgttccc tggccgacat tggtaactac attcggatat ggtttgatgt
3181 gctttgcaag gtatccggat cacatgaagc agcatgattt cttcaagtcc gccatgcccg
3241 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
3301 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
3361 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
3421 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
3481 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
3541 acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag
3601 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
3661 ctctcggcat ggacgagctg tacaagggat ccggtggcac cggtggatca ggctcttctc
3721 gaggttacta ccatggtatt tgggtgggtg aagaaggtcg actttctcga ggtggagaac
3781 aaaaacttat ttctgaagaa gatctgtgat ctagactcga gactagttct agagcggccg
3841 cggatcccgc ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata
3901 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
3961 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
4021 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
4081 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
4141 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
4201 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
4261 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
4321 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
4381 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taagcttgcc acaacccgta
4441 ccaaagatgg atagatccgg aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
4501 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
4561 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
4621 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
4681 gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca
4741 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
4801 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
4861 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
4921 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
4981 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
5041 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
5101 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
5161 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
5221 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
5281 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
5341 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
5401 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
5461 cgtccgaggg caaaggaata gacgcgtctg gaacaatcaa cctctggatt acaaaatttg
5521 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
5581 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
5641 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
5701 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
5761 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
5821 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
5881 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
5941 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
6001 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
6061 ctccctttgg gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat
6121 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
6181 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact
6241 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta
6301 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
6361 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga
6421 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag
6481 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg
6541 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg
6601 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt
6661 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa
6721 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
6781 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
6841 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
6901 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
6961 acttgcaaag aaatgaatat cagagagtga gaggccttga cattgctagc gtttaccgtc
7021 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
7081 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
7141 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
7201 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
7261 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
7321 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
7381 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
7441 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
7501 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
7561 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
7621 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
7681 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
7741 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
7801 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
7861 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
7921 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
7981 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
8041 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
8101 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
8161 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
8221 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
8281 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
8341 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
8401 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
8461 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
8521 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
8581 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
8641 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
8701 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
8761 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
8821 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
8881 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
8941 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
9001 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
9061 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
9121 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
9181 atttccccga aaagtgccac ctgacgtcga cggatcggga gatcaacttg tttattgcag
9241 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
9301 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca
9361 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca
9421 ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
9481 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt
9541 cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
9601 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
9661 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
9721 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
9781 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
9841 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
9901 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
9961 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
10021 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
10081 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
10141 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
10201 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
10261 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
10321 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
10381 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
10441 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
10501 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
10561 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
10621 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc
10681 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
10741 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
10801 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
10861 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
10921 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
10981 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
11041 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
11101 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
11161 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
11221 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
11281 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
11341 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
11401 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
11461 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
11521 ttcgattagt gaacggatct cgacggtatc gcctttaaaa gaaaaggggg gattgggggg
11581 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
11641 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
11701 gtttatcgat aagcttggga gttccg
//
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