| 质粒名称: | pMT-puro |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 17923 |
| 存储实验室: | David Sabatini |
|
载体骨架基本信息 |
|
| 载体名称: | pMT-puro |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 昆虫表达；果蝇金属硫蛋白基因启动子驱动表达 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | MT forward |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pMT-puro was a gift from David Sabatini (Addgene plasmid # 17923 ; http://n2t.net/addgene:17923 ; RRID:Addgene_17923) |
买家导航
pMTpuro载体基本信息
pMTpuro载体图谱质粒图谱和多克隆位点信息
pMTpuro载体简介
pMTpuro载体序列
LOCUS pMT-puro 4724 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Insect expression vector.
ACCESSION .
VERSION .
KEYWORDS pMT-puro
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4724)
TITLE Unpublished plasmid
REFERENCE 2 (bases 1 to 4724)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4724
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 2..24
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 16..33
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 17..33
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 39..465
/label=MT promoter
/note="Drosophila metallothionein promoter"
CDS 562..603
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
CDS 613..630
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(653..670)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 672..806
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(696..715)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 750..769
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
CDS complement(928..1527)
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(1011..1031)
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
primer_bind 1508..1527
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind complement(2141..2157)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2141..2157)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(2154..2176)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 2165..2181
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(2189..2219)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2234..2255
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(2372..2389)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2543..3131)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2623..2642)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3302..4162)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 3925..3944
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4163..4267)
/gene="bla"
/label=AmpR promoter
primer_bind 4335..4353
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(4391..4413)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 4513..4532
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 tcccagtcac gacgttgtaa aacgacggcc agtgaattaa ttcgttgcag gacaggatgt
61 ggtgcccgat gtgactagct ctttgctgca ggccgtccta tcctctggtt ccgataagag
121 acccagaact ccggcccccc accgcccacc gccaccccca tacatatgtg gtacgcaagt
181 aagagtgcct gcgcatgccc catgtgcccc accaagagct ttgcatccca tacaagtccc
241 caaagtggag aaccgaacca attcttcgcg ggcagaacaa aagcttctgc acacgtctcc
301 actcgaattt ggagccggcc ggcgtgtgca aaagaggtga atcgaacgaa agacccgtgt
361 gtaaagccgc gtttccaaaa tgtataaaac cgagagcatc tggccaatgt gcatcagttg
421 tggtcagcag caaaatcaag tgaatcatct cagtgcaact aaagggggga tctagatcgg
481 ggtacctact agtccagtgt ggtggaattc tgcagatatc cagcacagtg gcggccgctc
541 gagtctagag ggcccttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct
601 acgcgtaccg gtcatcatca ccatcaccat tgagtttaaa cccgctgatc agcctcgact
661 gtgccttcta agatccagac atgataagat acattgatga gtttggacaa accacaacta
721 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
781 ccattataag ctgcaataaa caagttctag agtcggtggg cctcgggggc gggtgcgggg
841 tcggcggggc cgccccgggt ggcttcggtc ggagccatgg ggtcgtgcgc tcctttcggt
901 cgggcgctgc gggtcgtggg gcgggcgtca ggcaccgggc ttgcgggtca tgcaccaggt
961 gcgcggtcct tcgggcacct cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa
1021 ggggaggttg cggggcgcgg aggtctccag gaaggcgggc accccggcgc gctcggccgc
1081 ctccactccg gggagcacga cggcgctgcc cagacccttg ccctggtggt cgggcgagac
1141 gccgacggtg gccaggaacc acgcgggctc cttgggccgg tgcggcgcca ggaggccttc
1201 catctgttgc tgcgcggcca gccgggaacc gctcaactcg gccatgcgcg ggccgatctc
1261 ggcgaacacc gcccccgctt cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc
1321 gtcgtccgcg acccacacct tgccgatgtc gagcccgacg cgcgtgagga agagttcttg
1381 cagctcggtg acccgctcga tgtggcggtc cgggtcgacg gtgtggcgcg tggcggggta
1441 gtcggcgaac gcggcggcga gggtgcgtac ggcccggggg acgtcgtcgc gggtggcgag
1501 gcgcaccgtg ggcttgtact cggtcatgga aggtcgtctc cttgtgaggg gtcaggggcg
1561 tgggtcaggg gatggtggcg gcaccggtcg tggcggccga cctgcaggca tgcaagctat
1621 cgaattcctg cagcccgggg gatctgttgt aatttataat ttatatttcc tttcttaata
1681 aataaataaa tagtcaagtt tatgtttgag ttttatgatt tatattttaa gttatttcaa
1741 ctgcaacacc agcaccacga cctacttaca gcaaaaaacg tacaagaagg aaagaaggaa
1801 taaaaagagt ggtattctct tacaatatgt tttatggcat aaaaggtgtg gccattcata
1861 tcaaatataa agtagtgttg tttaacgtta cttttgtagg ttgaatagta tattccaaca
1921 gatgatgagg ggttcccaat cctaaaccca tttgccgttc ccagaagcat gaaaccacca
1981 cgcaccggat cctctagaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
2041 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg
2101 atcagtcgac ctgcaggcat gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
2161 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
2221 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
2281 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
2341 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
2401 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
2461 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
2521 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
2581 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
2641 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
2701 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
2761 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
2821 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
2881 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
2941 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
3001 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
3061 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
3121 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
3181 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
3241 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
3301 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
3361 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
3421 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
3481 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
3541 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
3601 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
3661 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
3721 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
3781 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
3841 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
3901 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
3961 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
4021 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
4081 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
4141 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
4201 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
4261 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
4321 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
4381 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
4441 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
4501 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
4561 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc
4621 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
4681 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttt
//
