质粒名称: | pSIN REP5-GFP-GluR2 (Q) |
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出品公司: | Addgene |
目录编号: | 24003 |
存储实验室: |
Robert Malinow
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载体骨架基本信息 |
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载体名称: | pSIN |
载体出品公司: | -- |
空载体大小: | 7000 bp |
完整质粒大小: | 13753 bp |
载体修饰: | -- |
载体类型: | Sindbis virus production |
筛选标记: | -- |
载体抗性: | 氨苄青霉素,100μg/mL |
生长温度: | 37 ℃ |
宿主菌: | DH5a |
拷贝数: | -- |
插入基因信息 |
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插入基因名称: | GluR2 |
物种来源: | R. norvegicus (rat) |
插入大小: | -- |
基因突变: | R607Q |
GenBank编号 | -- |
启动子: | CMV |
标签/融合蛋白: | GFP(插入物上的N端) |
克隆方法: | 限制性内切酶 |
5’克隆位点: | -- |
3’克隆位点: | -- |
5’测序引物 | EGFP-C |
3’测序引物 | -- |
文献引用方法 |
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材料和方法部分: | pSIN REP5-GFP-GluR2 (Q) was a gift from Robert Malinow (Addgene plasmid # 24003 ; http://n2t.net/addgene:24003 ; RRID:Addgene_24003) |
参考文献部分: | Roles of stargazin and phosphorylation in the control of AMPA receptor subcellular distribution. Kessels HW, Kopec CD, Klein ME, Malinow R. Nat Neurosci. 2009 Jul . 12(7):888-96. 10.1038/nn.2340 PubMed 19543281 |
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载体基本信息
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载体简介
载体序列
LOCUS pSIN REP5-GFP-GluR2 (Q) 13753 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pSIN REP5-GFP-GluR2 (Q) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13753) AUTHORS Kessels HW, Kopec CD, Klein ME, Malinow R TITLE Roles of stargazin and phosphorylation in the control of AMPA receptor subcellular distribution. JOURNAL Nat Neurosci. 2009 Jul . 12(7):888-96. PUBMED 19543281 REFERENCE 2 (bases 1 to 13753) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.biofeng.com FEATURES Location/Qualifiers source 1..13753 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 7646..7662 /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind 7646..7662 /label=pBluescriptSK /note="For pBluescript vector" CDS 8069..8782 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" CDS 8069..8782 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" primer_bind complement(8111..8132) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(8372..8391) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 8719..8740 /label=EGFP-C /note="EGFP, forward primer" promoter 11924..12028 /gene="bla" /label=AmpR promoter CDS 12029..12889 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(12247..12266) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 13060..13648 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 13549..13568 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 attgacggcg tagtacacac tattgaatca aacagccgac caattgcact accatcacaa 61 tggagaagcc agtagtaaac gtagacgtag acccccagat ccgtttgtcg tgcaactgca 121 aaaaagcttc ccgcaatttg aggtagtagc acagcaggtc actccaaatg accatgctaa 181 tgccagagca ttttcgcatc tggccagtaa actaatcgag ctggaggttc ctaccacagc 241 gacgatcttg gacataggca gcgcaccggc tcgtagaatg ttttccgagc accagtatca 301 ttgtgtctgc cccatgcgta gtccagaaga cccggaccgc atgatgaaat acgccagtaa 361 actggcggaa aaagcgtgca agattacaaa caagaacttg catgagaaga ttaaggatct 421 ccggaccgta cttgatacgc cggatgctga aacaccatcg ctctgctttc acaacgatgt 481 tacctgcaac atgcgtgccg aatattccgt catgcaggac gtgtatatca acgctcccgg 541 aactatctat catcaggcta tgaaaggcgt gcggaccctg tactggattg gcttcgacac 601 cacccagttc atgttctcgg ctatggcagg ttcgtaccct gcgtacaaca ccaactgggc 661 cgacgagaaa gtccttgaag cgcgtaacat cggactttgc agcacaaagc tgagtgaagg 721 taggacagga aaattgtcga taatgaggaa gaaggagttg aagcccgggt cgcgggttta 781 tttctccgta ggatcgacac tttatccaga acacagagcc agcttgcaga gctggcatct 841 tccatcggtg ttccacttga atggaaagca gtcgtacact tgccgctgtg atacagtggt 901 gagttgcgaa ggctacgtag tgaagaaaat caccatcagt cccgggatca cgggagaaac 961 cgtgggatac gcggttacac acaatagcga gggcttcttg ctatgcaaag ttactgacac 1021 agtaaaagga gaacgggtat cgttccctgt gtgcacgtac atcccggcca ccatatgcga 1081 tcagatgact ggtataatgg ccacggatat atcacctgac gatgcacaaa aacttctggt 1141 tgggctcaac cagcgaattg tcattaacgg taggactaac aggaacacca acaccatgca 1201 aaattacctt ctgccgatca tagcacaagg gttcagcaaa tgggctaagg agcgcaagga 1261 tgatcttgat aacgagaaaa tgctgggtac tagagaacgc aagcttacgt atggctgctt 1321 gtgggcgttt cgcactaaga aagtacattc gttttatcgc ccacctggaa cgcagacctg 1381 cgtaaaagtc ccagcctctt ttagcgcttt tcccatgtcg tccgtatgga cgacctcttt 1441 gcccatgtcg ctgaggcaga aattgaaact ggcattgcaa ccaaagaagg aggaaaaact 1501 gctgcaggtc tcggaggaat tagtcatgga ggccaaggct gcttttgagg atgctcagga 1561 ggaagccaga gcggagaagc tccgagaagc acttccacca ttagtggcag acaaaggcat 1621 cgaggcagcc gcagaagttg tctgcgaagt ggaggggctc caggcggaca tcggagcagc 1681 attagttgaa accccgcgcg gtcacgtaag gataatacct caagcaaatg accgtatgat 1741 cggacagtat atcgttgtct cgccaaactc tgtgctgaag aatgccaaac tcgcaccagc 1801 gcacccgcta gcagatcagg ttaagatcat aacacactcc ggaagatcag gaaggtacgc 1861 ggtcgaacca tacgacgcta aagtactgat gccagcagga ggtgccgtac catggccaga 1921 attcctagca ctgagtgaga gcgccacgtt agtgtacaac gaaagagagt ttgtgaaccg 1981 caaactatac cacattgcca tgcatggccc cgccaagaat acagaagagg agcagtacaa 2041 ggttacaaag gcagagcttg cagaaacaga gtacgtgttt gacgtggaca agaagcgttg 2101 cgttaagaag gaagaagcct caggtctggt cctctcggga gaactgacca accctcccta 2161 tcatgagcta gctctggagg gactgaagac ccgacctgcg gtcccgtaca aggtcgaaac 2221 aataggagtg ataggcacac cggggtcggg caagtcagct attatcaagt caactgtcac 2281 ggcacgagat cttgttacca gcggaaagaa agaaaattgt cgcgaaattg aggccgacgt 2341 gctaagactg aggggtatgc agattacgtc gaagacagta gattcggtta tgctcaacgg 2401 atgccacaaa gccgtagaag tgctgtacgt tgacgaagcg ttcgcgtgcc acgcaggagc 2461 actacttgcc ttgattgcta tcgtcaggcc ccgcaagaag gtagtactat gcggagaccc 2521 catgcaatgc ggattcttca acatgatgca actaaaggta catttcaatc accctgaaaa 2581 agacatatgc accaagacat tctacaagta tatctcccgg cgttgcacac agccagttac 2641 agctattgta tcgacactgc attacgatgg aaagatgaaa accacgaacc cgtgcaagaa 2701 gaacattgaa atcgatatta caggggccac aaagccgaag ccaggggata tcatcctgac 2761 atgtttccgc gggtgggtta agcaattgca aatcgactat cccggacatg aagtaatgac 2821 agccgcggcc tcacaagggc taaccagaaa aggagtgtat gccgtccggc aaaaagtcaa 2881 tgaaaaccca ctgtacgcga tcacatcaga gcatgtgaac gtgttgctca cccgcactga 2941 ggacaggcta gtgtggaaaa ccttgcaggg cgacccatgg attaagcagc ccactaacat 3001 acctaaagga aactttcagg ctactataga ggactgggaa gctgaacaca agggaataat 3061 tgctgcaata aacagcccca ctccccgtgc caatccgttc agctgcaaga ccaacgtttg 3121 ctgggcgaaa gcattggaac cgatactagc cacggccggt atcgtactta ccggttgcca 3181 gtggagcgaa ctgttcccac agtttgcgga tgacaaacca cattcggcca tttacgcctt 3241 agacgtaatt tgcattaagt ttttcggcat ggacttgaca agcggactgt tttctaaaca 3301 gagcatccca ctaacgtacc atcccgccga ttcagcgagg ccggtagctc attgggacaa 3361 cagcccagga acccgcaagt atgggtacga tcacgccatt gccgccgaac tctcccgtag 3421 atttccggtg ttccagctag ctgggaaggg cacacaactt gatttgcaga cggggagaac 3481 cagagttatc tctgcacagc ataacctggt cccggtgaac cgcaatcttc ctcacgcctt 3541 agtccccgag tacaaggaga agcaacccgg cccggtcaaa aaattcttga accagttcaa 3601 acaccactca gtacttgtgg tatcagagga aaaaattgaa gctccccgta agagaatcga 3661 atggatcgcc ccgattggca tagccggtgc agataagaac tacaacctgg ctttcgggtt 3721 tccgccgcag gcacggtacg acctggtgtt catcaacatt ggaactaaat acagaaacca 3781 ccactttcag cagtgcgaag accatgcggc gaccttaaaa accctttcgc gttcggccct 3841 gaattgcctt aacccaggag gcaccctcgt ggtgaagtcc tatggctacg ccgaccgcaa 3901 cagtgaggac gtagtcaccg ctcttgccag aaagtttgtc agggtgtctg cagcgagacc 3961 agattgtgtc tcaagcaata cagaaatgta cctgattttc cgacaactag acaacagccg 4021 tacacggcaa ttcaccccgc accatctgaa ttgcgtgatt tcgtccgtgt atgagggtac 4081 aagagatgga gttggagccg cgccgtcata ccgcaccaaa agggagaata ttgctgactg 4141 tcaagaggaa gcagttgtca acgcagccaa tccgctgggt agaccaggcg aaggagtctg 4201 ccgtgccatc tataaacgtt ggccgaccag ttttaccgat tcagccacgg agacaggcac 4261 cgcaagaatg actgtgtgcc taggaaagaa agtgatccac gcggtcggcc ctgatttccg 4321 gaagcaccca gaagcagaag ccttgaaatt gctacaaaac gcctaccatg cagtggcaga 4381 cttagtaaat gaacataaca tcaagtctgt cgccattcca ctgctatcta caggcattta 4441 cgcagccgga aaagaccgcc ttgaagtatc acttaactgc ttgacaaccg cgctagacag 4501 aactgacgcg gacgtaacca tctattgcct ggataagaag tggaaggaaa gaatcgacgc 4561 ggcactccaa cttaaggagt ctgtaacaga gctgaaggat gaagatatgg agatcgacga 4621 tgagttagta tggattcatc cagacagttg cttgaaggga agaaagggat tcagtactac 4681 aaaaggaaaa ttgtattcgt acttcgaagg caccaaattc catcaagcag caaaagacat 4741 ggcggagata aaggtcctgt tccctaatga ccaggaaagt aatgaacaac tgtgtgccta 4801 catattgggt gagaccatgg aagcaatccg cgaaaagtgc ccggtcgacc ataacccgtc 4861 gtctagcccg cccaaaacgt tgccgtgcct ttgcatgtat gccatgacgc cagaaagggt 4921 ccacagactt agaagcaata acgtcaaaga agttacagta tgctcctcca ccccccttcc 4981 taagcacaaa attaagaatg ttcagaaggt tcagtgcacg aaagtagtcc tgtttaatcc 5041 gcacactccc gcattcgttc ccgcccgtaa gtacatagaa gtgccagaac agcctaccgc 5101 tcctcctgca caggccgagg aggcccccga agttgtagcg acaccgtcac catctacagc 5161 tgataacacc tcgcttgatg tcacagacat ctcactggat atggatgaca gtagcgaagg 5221 ctcacttttt tcgagcttta gcggatcgga caactctatt actagtatgg acagttggtc 5281 gtcaggacct agttcactag agatagtaga ccgaaggcag gtggtggtgg ctgacgttca 5341 tgccgtccaa gagcctgccc ctattccacc gccaaggcta aagaagatgg cccgcctggc 5401 agcggcaaga aaagagccca ctccaccggc aagcaatagc tctgagtccc tccacctctc 5461 ttttggtggg gtatccatgt ccctcggatc aattttcgac ggagagacgg cccgccaggc 5521 agcggtacaa cccctggcaa caggccccac ggatgtgcct atgtctttcg gatcgttttc 5581 cgacggagag attgatgagc tgagccgcag agtaactgag tccgaacccg tcctgtttgg 5641 atcatttgaa ccgggcgaag tgaactcaat tatatcgtcc cgatcagccg tatcttttcc 5701 actacgcaag cagagacgta gacgcaggag caggaggact gaatactgac taaccggggt 5761 aggtgggtac atattttcga cggacacagg ccctgggcac ttgcaaaaga agtccgttct 5821 gcagaaccag cttacagaac cgaccttgga gcgcaatgtc ctggaaagaa ttcatgcccc 5881 ggtgctcgac acgtcgaaag aggaacaact caaactcagg taccagatga tgcccaccga 5941 agccaacaaa agtaggtacc agtctcgtaa agtagaaaat cagaaagcca taaccactga 6001 gcgactactg tcaggactac gactgtataa ctctgccaca gatcagccag aatgctataa 6061 gatcacctat ccgaaaccat tgtactccag tagcgtaccg gcgaactact ccgatccaca 6121 gttcgctgta gctgtctgta acaactatct gcatgagaac tatccgacag tagcatctta 6181 tcagattact gacgagtacg atgcttactt ggatatggta gacgggacag tcgcctgcct 6241 ggatactgca accttctgcc ccgctaagct tagaagttac ccgaaaaaac atgagtatag 6301 agccccgaat atccgcagtg cggttccatc agcgatgcag aacacgctac aaaatgtgct 6361 cattgccgca actaaaagaa attgcaacgt cacgcagatg cgtgaactgc caacactgga 6421 ctcagcgaca ttcaatgtcg aatgctttcg aaaatatgca tgtaatgacg agtattggga 6481 ggagttcgct cggaagccaa ttaggattac cactgagttt gtcaccgcat atgtagctag 6541 actgaaaggc cctaaggccg ccgcactatt tgcaaagacg tataatttgg tcccattgca 6601 agaagtgcct atggatagat tcgtcatgga catgaaaaga gacgtgaaag ttacaccagg 6661 cacgaaacac acagaagaaa gaccgaaagt acaagtgata caagccgcag aacccctggc 6721 gactgcttac ttatgcggga ttcaccggga attagtgcgt aggcttacgg ccgtcttgct 6781 tccaaacatt cacacgcttt ttgacatgtc ggcggaggat tttgatgcaa tcatagcaga 6841 acacttcaag caaggcgacc cggtactgga gacggatatc gcatcattcg acaaaagcca 6901 agacgacgct atggcgttaa ccggtctgat gatcttggag gacctgggtg tggatcaacc 6961 actactcgac ttgatcgagt gcgcctttgg agaaatatca tccacccatc tacctacggg 7021 tactcgtttt aaattcgggg cgatgatgaa atccggaatg ttcctcacac tttttgtcaa 7081 cacagttttg aatgtcgtta tcgccagcag agtactagaa gagcggctta aaacgtccag 7141 atgtgcagcg ttcattggcg acgacaacat catacatgga gtagtatctg acaaagaaat 7201 ggctgagagg tgcgccacct ggctcaacat ggaggttaag atcatcgacg cagtcatcgg 7261 tgagagacca ccttacttct gcggcggatt tatcttgcaa gattcggtta cttccacagc 7321 gtgccgcgtg gcggatcccc tgaaaaggct gtttaagttg ggtaaaccgc tcccagccga 7381 cgacgagcaa gacgaagaca gaagacgcgc tctgctagat gaaacaaagg cgtggtttag 7441 agtaggtata acaggcactt tagcagtggc cgtgacgacc cggtatgagg tagacaatat 7501 tacacctgtc ctactggcat tgagaacttt tgcccagagc aaaagagcat tccaagccat 7561 cagaggggaa ataaagcatc tctacggtgg tcctaaatag tcagcatagt acatttcatc 7621 tgactaatac tacaacacca ccacctctag aactagtgga tcccccgggc tgcaggaatt 7681 cggcacgagg tgcatgggag ggtgctgata ttcccagaca ccaggactac agcggcagct 7741 cagctaaaaa ctgcattcag ccagtcctcg ggacttcggg agcagggaca ggacgcaagg 7801 catcaacagc caccagctac aactgggaaa taaggattct tctgccttca cttcgtgttt 7861 ttagcagctc cttgctaaat atcgacctca caatgcagag gatctaattt gctgaggaaa 7921 acagtcaaag aaggaagagg aagaaaggga aacgagggga tattttgtgg atgctctact 7981 tttcttggaa atgcaaaaga ttatgcatat ttctgtcctc ctttctcctg ttttatgggg 8041 actgattttt ggtgtctctt ctgctagggt gagcaagggc gaggagctgt tcaccggggt 8101 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg 8161 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg 8221 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt 8281 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg 8341 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga 8401 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa 8461 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta 8521 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat 8581 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg 8641 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc 8701 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct 8761 cggcatggac gagctgtaca aggctagcaa cagcatacag ataggggggc tatttccaag 8821 gggcgctgat caagaataca gtgcatttcg ggtagggatg gttcagtttt ccacttcgga 8881 gttcagactg acaccccata tcgacaattt ggaggtagcc aacagtttcg cagtcaccaa 8941 tgctttctgc tcccagtttt caagaggagt ctacgcaatt tttggatttt atgacaagaa 9001 gtctgtaaat accatcacat cattctgtgg gacactccat gtgtccttca tcacacctag 9061 cttcccaaca gatggcacac atccatttgt catccagatg cgacctgacc tcaaaggagc 9121 actccttagc ttgattgagt actaccaatg ggacaagttc gcatacctct atgacagtga 9181 cagaggctta tcaacactgc aagctgttct ggattctgct gcagagaaga agtggcaggt 9241 gactgctatc aatgtgggga acatcaacaa tgacaagaaa gatgagacct acagatcgct 9301 ctttcaagat ctggagttaa aaaaagaacg gcgtgtaatc ctggactgtg aaagggataa 9361 agtaaatgac attgtggacc aggttattac cattggaaaa catgttaaag ggtaccatta 9421 tatcattgca aatctgggat tcactgatgg ggacctgctg aaaattcagt ttggaggagc 9481 aaatgtctct ggatttcaga ttgtagacta cgacgattcc ctggtgtcta aatttataga 9541 aagatggtca acactggaag agaaagaata ccctggagca cacacagcga caattaagta 9601 tacttcggcc ctgacgtatg atgctgtcca agtgatgact gaagcattcc gtaaccttcg 9661 gaagcagagg attgaaatat cccggagagg aaatgcaggg gattgtttgg ccaacccagc 9721 tgtgccctgg ggacaagggg tcgaaataga aagggccctc aagcaggttc aagttgaagg 9781 cctctctgga aatataaagt ttgaccagaa tggaaaacga ataaactaca caattaacat 9841 catggagctc aaaacaaatg gaccccggaa gattgggtac tggagtgaag tggataaaat 9901 ggttgtcacc ctaactgagc tcccatcagg aaatgacacg tctgggcttg aaaacaaaac 9961 tgtggtggtc accacaatat tggaatctcc atatgttatg atgaagaaaa atcatgaaat 10021 gcttgaaggg aatgagcgtt acgagggcta ctgtgttgac ttagctgcag aaattgccaa 10081 acactgtggg ttcaagtaca agctgactat tgttggggat ggcaagtatg gggccaggga 10141 tgccgacacc aaaatttgga atggtatggt tggagagctt gtctacggga aagctgacat 10201 tgcaattgct ccattaacta tcactctcgt gagagaagag gtgattgact tctccaagcc 10261 cttcatgagt cttggaatct ctatcatgat caagaagcct cagaagtcca aaccaggagt 10321 gttttccttt cttgatcctt tagcctatga gatctggatg tgcattgtgt ttgcctacat 10381 tggggtcagt gtagttttat tcctggtcag cagatttagc ccctacgagt ggcacactga 10441 ggaatttgaa gatggaagag aaacacaaag tagtgaatca actaatgaat ttgggatttt 10501 taatagtctc tggttttcct tgggtgcctt tatgcagcag ggatgcgata tttcgccaag 10561 atccctctct gggcgcattg ttggaggtgt gtggtggttc tttaccctga tcataatctc 10621 ctcctacacg gctaacttag ctgccttcct gactgtagag aggatggtgt ctcccatcga 10681 aagtgctgag gatctgtcta agcaaacaga aattgcttat ggaacattag actctggctc 10741 cactaaagag tttttcagga gatctaaaat cgcagtgttt gataaaatgt ggacttatat 10801 gaggagtgca gagccctctg tgtttgtgag gactaccgca gaaggagtag ccagagtccg 10861 gaaatccaaa ggaaagtatg cctacttgct ggagtccaca atgaacgagt acatcgagca 10921 gaggaagcct tgtgacacca tgaaagtggg aggaaacttg gattccaaag gctacggcat 10981 cgccacacct aaaggatcct cattaggaaa tgcggttaac ctcgcagtac taaaactgaa 11041 tgaacaaggc ctgttggaca aattgaaaaa caaatggtgg tacgacaaag gagagtgcgg 11101 cagcggggga ggtgattcca aggaaaagac cagtgccctc agtctgagca acgttgctgg 11161 agtattctac atccttgtcg ggggccttgg tttggcaatg ctggtggctt tgattgagtt 11221 ctgttacaag tcaagggccg aggcgaaacg aatgaaggtg gcaaagaatc cacagaatat 11281 taacccatct tcctcgcaga attcccagaa ttttgcaact tataaggaag gttacaacgt 11341 atatggcatc gagagtgtta aaatttaggg gatgaccttg agtgatgtca tgaggagcaa 11401 ggcaaggctg tcaattacag gaagtactgg agaaaatgga cgtgttatga ctccagaatt 11461 tcccaaagca gtgcatgcag gccttgggcc caatgatccg accagcaaaa ctcgatgtac 11521 ttccgaggaa ctgatgtgca taatgcatca ggctggtaca ttagatcccc gcttaccgcg 11581 ggcaatatag caacactaaa aactcgatgt acttccgagg aagcgcagtg cataatgctg 11641 cgcagtgttg ccacataacc actatattaa ccatttatct agcggacgcc aaaaactcaa 11701 tgtatttctg aggaagcgtg gtgcataatg ccacgcagcg tctgcataac ttttattatt 11761 tcttttatta atcaacaaaa ttttgttttt aacatttcaa aaaaaaaaaa aaaaaaaaaa 11821 aaaaaaaaaa aaaaagggaa ttcctcgatt aattaagcgg ccgctcgagg ggaattaatt 11881 cttgaagacg aaagggccag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 11941 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 12001 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 12061 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 12121 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 12181 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 12241 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 12301 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 12361 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 12421 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 12481 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 12541 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 12601 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 12661 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 12721 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 12781 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 12841 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 12901 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 12961 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 13021 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 13081 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 13141 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 13201 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 13261 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 13321 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 13381 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 13441 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 13501 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 13561 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 13621 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcgagctcgt atggacatat 13681 tgtcgttaga acgcggctac aattaataca taaccttatg tatcatacac atacgattta 13741 ggggacacta tag //