pSQT1601

价格:1200元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

买家导航

载体基本信息

质粒名称: pSQT1601 
出品公司: 爱迪基因
目录编号: 53369
存储实验室: Keith Joung

载体骨架基本信息
载体名称: pCAG-CFP
载体抗性: 氨苄霉素
载体类型: 哺乳细胞表达
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: hCsy4-T2A-NLS-hFokI-dCas9-NLS
启动子 CAG
克隆方法: 未知
5' 测序引物 pCAG-F
3' 测序引物 Bglob-pA-R

文献引用方法
材料和方法部分: pSQT1601 was a gift from Keith Joung (Addgene plasmid # 53369)
参考文献部分: Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing. Tsai SQ, Wyvekens N, Khayter C, Foden JA, Thapar V, Reyon D, Goodwin MJ, Aryee MJ, Joung JK. Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908. 10.1038/nbt.2908 PubMed 24770325

载体图谱质粒图谱和多克隆位点信息

pSQT1601载体图谱

载体简介

载体序列

LOCUS       pSQT1601 Sequencing Result	10323 bp 	DNA	SYN
DEFINITION  pSQT1601 Sequencing Result
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..10323
                     /organism="pSQT1601 Sequencing Result"
                     /mol_type="other DNA"
     gene            complement(993..1853)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(993..1853)
                     /label="ORF frame 2"
     promoter        complement(1895..1923)
                     /label="AmpR_promoter"
     misc_feature    2069..2356
                     /label="CAG_enhancer"
     CDS             complement(2703..3311)
                     /label="ORF frame 2"
     misc_feature    3646..3665
                     /label="pCAG_F_primer"
     CDS             complement(3674..4201)
                     /label="ORF frame 3"
     CDS             3737..9190
                     /label="ORF frame 2"
     misc_feature    4358..4378
                     /label="SV40 NLS"
     misc_feature    4364..4378
                     /label="NLS"
     misc_feature    9122..9142
                     /label="FLAG"
     misc_feature    9143..9163
                     /label="FLAG"
     misc_feature    9164..9187
                     /label="FLAG"
     misc_feature    9173..9187
                     /label="EK"
     terminator      9209..9723
                     /label="rb_glob_PA_terminator"
     promoter        complement(9754..9772)
                     /label="M13_reverse_primer"
     misc_feature    complement(9771..9793)
                     /label="M13_pUC_rev_primer"
     promoter        complement(9807..9836)
                     /label="lac_promoter"
     promoter        9942..10121
                     /label="SV40_promoter"
     rep_origin      9977..10054
                     /label="SV40_origin"
     misc_feature    10039..10058
                     /label="SV40pro_F_primer"
     terminator      10135..10266
                     /label="SV40_PA_terminator"
     misc_feature    10223..10242
                     /label="EBV_rev_primer"
ORIGIN
    1 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctCTTCCGCT
   61 TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC
  121 TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA
  181 GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT
  241 AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC
  301 CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT
  361 GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG
  421 CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG
  481 GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT
  541 CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG
  601 ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC
  661 GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA
  721 AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT
  781 GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT
  841 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA
  901 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC
  961 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT
 1021 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA
 1081 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA
 1141 CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA
 1201 AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA
 1261 GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG
 1321 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA
 1381 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT
 1441 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT
 1501 CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA
 1561 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT
 1621 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA
 1681 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC
 1741 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG
 1801 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC
 1861 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
 1921 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
 1981 CCTGGGTCGA CATTGATTAT TGACTAGTTA TTAATAGTAA TCAATTACGG GGTCATTAGT
 2041 TCATAGCCCA TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC CGCCTGGCTG
 2101 ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA TAGTAACGCC
 2161 AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG CCCACTTGGC
 2221 AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG ACGGTAAATG
 2281 GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT GGCAGTACAT
 2341 CTACGTATTA GTCATCGCTA TTACCATGGT CGAGGTGAGC CCCACGTTCT GCTTCACTCT
 2401 CCCCATCTCC CCCCCCTCCC CACCCCCAAT TTTGTATTTA TTTATTTTTT AATTATTTTG
 2461 TGCAGCGATG GGGGCGGGGG GGGGGGGGGG GCGAGGGGCG GGGCGGGGCG AGGCGGAGAG
 2521 GTGCGGCGGC AGCCAATCAG AGCGGCGCGC TCCGAAAGTT TCCTTTTATG GCGAGGCGGC
 2581 GGCGGCGGCG GCCCTATAAA AAGCGAAGCG CGCGGCGGGC GGGAGTCGCT GCGCGCTGCC
 2641 TTCGCCCCGT GCCCCGCTCC GCCGCCGCCT CGCGCCGCCC GCCCCGGCTC TGACTGACCG
 2701 CGTTACTCCC ACAGGTGAGC GGGCGGGACG GCCCTTCTCC TCCGGGCTGT AATTAGCGCT
 2761 TGGTTTAATG ACGGCTTGTT TCTTTTCTGT GGCTGCGTGA AAGCCTTGAG GGGCTCCGGG
 2821 AGGGCCCTTT GTGCGGGGGG AGCGGCTCGG GGGGTGCGTG CGTGTGTGTG TGCGTGGGGA
 2881 GCGCCGCGTG CGGCTCCGCG CTGCCCGGCG GCTGTGAGCG CTGCGGGCGC GGCGCGGGGC
 2941 TTTGTGCGCT CCGCAGTGTG CGCGAGGGGA GCGCGGCCGG GGGCGGTGCC CCGCGGTGCG
 3001 GGGGGGGCTG CGAGGGGAAC AAAGGCTGCG TGCGGGGTGT GTGCGTGGGG GGGTGAGCAG
 3061 GGGGTGTGGG CGCGTCGGTC GGGCTGCAAC CCCCCCTGCA CCCCCCTCCC CGAGTTGCTG
 3121 AGCACGGCCC GGCTTCGGGT GCGGGGCTCC GTACGGGGCG TGGCGCGGGG CTCGCCGTGC
 3181 CGGGCGGGGG GTGGCGGCAG GTGGGGGTGC CGGGCGGGGC GGGGCCGCCT CGGGCCGGGG
 3241 AGGGCTCGGG GGAGGGGCGC GGCGGCCCCC GGAGCGCCGG CGGCTGTCGA GGCGCGGCGA
 3301 GCCGCAGCCA TTGCCTTTTA TGGTAATCGT GCGAGAGGGC GCAGGGACTT CCTTTGTCCC
 3361 AAATCTGTGC GGAGCCGAAA TCTGGGAGGC GCCGCCGCAC CCCCTCTAGC GGGCGCGGGG
 3421 CGAAGCGGTG CGGCGCCGGC AGGAAGGAAA TGGGCGGGGA GGGCCTTCGT GCGTCGCCGC
 3481 GCCGCCGTCC CCTTCTCCCT CTCCAGCCTC GGGGCTGTCC GCGGGGGGAC GGCTGCCTTC
 3541 GGGGGGGACG GGGCAGGGCG GGGTTCGGCT TCTGGCGTGT GACCGGCGGC TCTAGAGCCT
 3601 CTGCTAACCA TGTTCATGCC TTCTTCTTTT TCCTACAGCT CCTGGGCAAC GTGCTGGTTA
 3661 TTGTGCTGTC TCATCATTTT GGCAAAGAAT TCTGCAGTCG ACGGTACCGC GGGCCCGGGA
 3721 TCCACCGGTC GCCACCATGG GTGATCATTA TCTGGATATT CGGCTGAGGC CTGATCCAGA
 3781 GTTCCCACCT GCGCAGCTGA TGTCTGTCCT TTTTGGCAAA CTTCATCAGG CCCTGGTTGC
 3841 CCAGGGCGGA GATCGGATAG GGGTAAGCTT TCCAGACCTC GACGAAAGCC GGAGCCGCCT
 3901 GGGAGAACGC CTGCGGATCC ACGCTTCTGC CGACGATCTG AGAGCCTTGC TGGCAAGGCC
 3961 ATGGCTTGAG GGGCTCCGGG ATCACCTGCA GTTTGGCGAA CCCGCCGTTG TTCCCCACCC
 4021 AACCCCTTAT CGGCAGGTGT CTAGAGTGCA GGCCAAATCT AATCCAGAAC GGCTGCGACG
 4081 GCGACTCATG CGGCGACATG ATCTTAGCGA GGAAGAGGCC CGAAAAAGAA TCCCTGATAC
 4141 CGTGGCCCGC GCCCTTGACT TGCCTTTTGT CACACTGCGG TCCCAGAGTA CGGGGCAGCA
 4201 TTTCAGACTT TTCATTCGAC ACGGGCCACT GCAAGTTACC GCCGAAGAAG GAGGCTTTAC
 4261 TTGTTATGGA CTCTCCAAGG GAGGTTTCGT GCCCTGGTTT GAGGGCAGAG GAAGTCTGTT
 4321 AACATGCGGT GACGTCGAGG AGAATCCTGG CCCAATGCCT AAGAAGAAGC GGAAGGTGAG
 4381 CAGCCAACTT GTGAAGTCTG AACTCGAGGA GAAAAAATCA GAGTTGAGAC ACAAGTTGAA
 4441 GTACGTGCCA CACGAATACA TCGAGCTTAT CGAGATCGCC AGAAACAGTA CCCAGGATAG
 4501 GATCCTTGAG ATGAAAGTCA TGGAGTTCTT TATGAAGGTC TACGGTTATA GAGGAAAGCA
 4561 CCTTGGCGGT AGCAGAAAGC CCGATGGCGC CATCTATACT GTCGGATCTC CTATCGATTA
 4621 TGGGGTGATC GTGGATACCA AAGCTTACTC AGGCGGGTAC AACTTGCCCA TAGGACAAGC
 4681 CGACGAGATG CAGCGGTATG TCGAAGAGAA CCAGACGCGC AACAAGCACA TCAACCCCAA
 4741 TGAATGGTGG AAAGTGTACC CAAGTAGTGT GACTGAGTTC AAGTTCCTGT TTGTCTCCGG
 4801 CCACTTTAAG GGCAATTATA AAGCTCAGCT CACTAGACTC AATCACATCA CAAACTGCAA
 4861 CGGAGCTGTG TTGTCAGTGG AGGAGCTCCT GATTGGAGGC GAGATGATCA AAGCCGGCAC
 4921 CCTTACACTG GAGGAGGTGC GGCGGAAGTT CAACAATGGA GAGATCAACT TCGGTGGCGG
 4981 TGGATCCGAT AAAAAGTATT CTATTGGTTT AGCCATCGGC ACTAATTCCG TTGGATGGGC
 5041 TGTCATAACC GATGAATACA AAGTACCTTC AAAGAAATTT AAGGTGTTGG GGAACACAGA
 5101 CCGTCATTCG ATTAAAAAGA ATCTTATCGG TGCCCTCCTA TTCGATAGTG GCGAAACGGC
 5161 AGAGGCGACT CGCCTGAAAC GAACCGCTCG GAGAAGGTAT ACACGTCGCA AGAACCGAAT
 5221 ATGTTACTTA CAAGAAATTT TTAGCAATGA GATGGCCAAA GTTGACGATT CTTTCTTTCA
 5281 CCGTTTGGAA GAGTCCTTCC TTGTCGAAGA GGACAAGAAA CATGAACGGC ACCCCATCTT
 5341 TGGAAACATA GTAGATGAGG TGGCATATCA TGAAAAGTAC CCAACGATTT ATCACCTCAG
 5401 AAAAAAGCTA GTTGACTCAA CTGATAAAGC GGACCTGAGG TTAATCTACT TGGCTCTTGC
 5461 CCATATGATA AAGTTCCGTG GGCACTTTCT CATTGAGGGT GATCTAAATC CGGACAACTC
 5521 GGATGTCGAC AAACTGTTCA TCCAGTTAGT ACAAACCTAT AATCAGTTGT TTGAAGAGAA
 5581 CCCTATAAAT GCAAGTGGCG TGGATGCGAA GGCTATTCTT AGCGCCCGCC TCTCTAAATC
 5641 CCGACGGCTA GAAAACCTGA TCGCACAATT ACCCGGAGAG AAGAAAAATG GGTTGTTCGG
 5701 TAACCTTATA GCGCTCTCAC TAGGCCTGAC ACCAAATTTT AAGTCGAACT TCGACTTAGC
 5761 TGAAGATGCC AAATTGCAGC TTAGTAAGGA CACGTACGAT GACGATCTCG ACAATCTACT
 5821 GGCACAAATT GGAGATCAGT ATGCGGACTT ATTTTTGGCT GCCAAAAACC TTAGCGATGC
 5881 AATCCTCCTA TCTGACATAC TGAGAGTTAA TACTGAGATT ACCAAGGCGC CGTTATCCGC
 5941 TTCAATGATC AAAAGGTACG ATGAACATCA CCAAGACTTG ACACTTCTCA AGGCCCTAGT
 6001 CCGTCAGCAA CTGCCTGAGA AATATAAGGA AATATTCTTT GATCAGTCGA AAAACGGGTA
 6061 CGCAGGTTAT ATTGACGGCG GAGCGAGTCA AGAGGAATTC TACAAGTTTA TCAAACCCAT
 6121 ATTAGAGAAG ATGGATGGGA CGGAAGAGTT GCTTGTAAAA CTCAATCGCG AAGATCTACT
 6181 GCGAAAGCAG CGGACTTTCG ACAACGGTAG CATTCCACAT CAAATCCACT TAGGCGAATT
 6241 GCATGCTATA CTTAGAAGGC AGGAGGATTT TTATCCGTTC CTCAAAGACA ATCGTGAAAA
 6301 GATTGAGAAA ATCCTAACCT TTCGCATACC TTACTATGTG GGACCCCTGG CCCGAGGGAA
 6361 CTCTCGGTTC GCATGGATGA CAAGAAAGTC CGAAGAAACG ATTACTCCAT GGAATTTTGA
 6421 GGAAGTTGTC GATAAAGGTG CGTCAGCTCA ATCGTTCATC GAGAGGATGA CCAACTTTGA
 6481 CAAGAATTTA CCGAACGAAA AAGTATTGCC TAAGCACAGT TTACTTTACG AGTATTTCAC
 6541 AGTGTACAAT GAACTCACGA AAGTTAAGTA TGTCACTGAG GGCATGCGTA AACCCGCCTT
 6601 TCTAAGCGGA GAACAGAAGA AAGCAATAGT AGATCTGTTA TTCAAGACCA ACCGCAAAGT
 6661 GACAGTTAAG CAATTGAAAG AGGACTACTT TAAGAAAATT GAATGCTTCG ATTCTGTCGA
 6721 GATCTCCGGG GTAGAAGATC GATTTAATGC GTCACTTGGT ACGTATCATG ACCTCCTAAA
 6781 GATAATTAAA GATAAGGACT TCCTGGATAA CGAAGAGAAT GAAGATATCT TAGAAGATAT
 6841 AGTGTTGACT CTTACCCTCT TTGAAGATCG GGAAATGATT GAGGAAAGAC TAAAAACATA
 6901 CGCTCACCTG TTCGACGATA AGGTTATGAA ACAGTTAAAG AGGCGTCGCT ATACGGGCTG
 6961 GGGACGATTG TCGCGGAAAC TTATCAACGG GATAAGAGAC AAGCAAAGTG GTAAAACTAT
 7021 TCTCGATTTT CTAAAGAGCG ACGGCTTCGC CAATAGGAAC TTTATGCAGC TGATCCATGA
 7081 TGACTCTTTA ACCTTCAAAG AGGATATACA AAAGGCACAG GTTTCCGGAC AAGGGGACTC
 7141 ATTGCACGAA CATATTGCGA ATCTTGCTGG TTCGCCAGCC ATCAAAAAGG GCATACTCCA
 7201 GACAGTCAAA GTAGTGGATG AGCTAGTTAA GGTCATGGGA CGTCACAAAC CGGAAAACAT
 7261 TGTAATCGAG ATGGCACGCG AAAATCAAAC GACTCAGAAG GGGCAAAAAA ACAGTCGAGA
 7321 GCGGATGAAG AGAATAGAAG AGGGTATTAA AGAACTGGGC AGCCAGATCT TAAAGGAGCA
 7381 TCCTGTGGAA AATACCCAAT TGCAGAACGA GAAACTTTAC CTCTATTACC TACAAAATGG
 7441 AAGGGACATG TATGTTGATC AGGAACTGGA CATAAACCGT TTATCTGATT ACGACGTCGA
 7501 TGCCATTGTA CCCCAATCCT TTTTGAAGGA CGATTCAATC GACAATAAAG TGCTTACACG
 7561 CTCGGATAAG AACCGAGGGA AAAGTGACAA TGTTCCAAGC GAGGAAGTCG TAAAGAAAAT
 7621 GAAGAACTAT TGGCGGCAGC TCCTAAATGC GAAACTGATA ACGCAAAGAA AGTTCGATAA
 7681 CTTAACTAAA GCTGAGAGGG GTGGCTTGTC TGAACTTGAC AAGGCCGGAT TTATTAAACG
 7741 TCAGCTCGTG GAAACCCGCC AAATCACAAA GCATGTTGCA CAGATACTAG ATTCCCGAAT
 7801 GAATACGAAA TACGACGAGA ACGATAAGCT GATTCGGGAA GTCAAAGTAA TCACTTTAAA
 7861 GTCAAAATTG GTGTCGGACT TCAGAAAGGA TTTTCAATTC TATAAAGTTA GGGAGATAAA
 7921 TAACTACCAC CATGCGCACG ACGCTTATCT TAATGCCGTC GTAGGGACCG CACTCATTAA
 7981 GAAATACCCG AAGCTAGAAA GTGAGTTTGT GTATGGTGAT TACAAAGTTT ATGACGTCCG
 8041 TAAGATGATC GCGAAAAGCG AACAGGAGAT AGGCAAGGCT ACAGCCAAAT ACTTCTTTTA
 8101 TTCTAACATT ATGAATTTCT TTAAGACGGA AATCACTCTG GCAAACGGAG AGATACGCAA
 8161 ACGACCTTTA ATTGAAACCA ATGGGGAGAC AGGTGAAATC GTATGGGATA AGGGCCGGGA
 8221 CTTCGCGACG GTGAGAAAAG TTTTGTCCAT GCCCCAAGTC AACATAGTAA AGAAAACTGA
 8281 GGTGCAGACC GGAGGGTTTT CAAAGGAATC GATTCTTCCA AAAAGGAATA GTGATAAGCT
 8341 CATCGCTCGT AAAAAGGACT GGGACCCGAA AAAGTACGGT GGCTTCGATA GCCCTACAGT
 8401 TGCCTATTCT GTCCTAGTAG TGGCAAAAGT TGAGAAGGGA AAATCCAAGA AACTGAAGTC
 8461 AGTCAAAGAA TTATTGGGGA TAACGATTAT GGAGCGCTCG TCTTTTGAAA AGAACCCCAT
 8521 CGACTTCCTT GAGGCGAAAG GTTACAAGGA AGTAAAAAAG GATCTCATAA TTAAACTACC
 8581 AAAGTATAGT CTGTTTGAGT TAGAAAATGG CCGAAAACGG ATGTTGGCTA GCGCCGGAGA
 8641 GCTTCAAAAG GGGAACGAAC TCGCACTACC GTCTAAATAC GTGAATTTCC TGTATTTAGC
 8701 GTCCCATTAC GAGAAGTTGA AAGGTTCACC TGAAGATAAC GAACAGAAGC AACTTTTTGT
 8761 TGAGCAGCAC AAACATTATC TCGACGAAAT CATAGAGCAA ATTTCGGAAT TCAGTAAGAG
 8821 AGTCATCCTA GCTGATGCCA ATCTGGACAA AGTATTAAGC GCATACAACA AGCACAGGGA
 8881 TAAACCCATA CGTGAGCAGG CGGAAAATAT TATCCATTTG TTTACTCTTA CCAACCTCGG
 8941 CGCTCCAGCC GCATTCAAGT ATTTTGACAC AACGATAGAT CGCAAACGAT ACACTTCTAC
 9001 CAAGGAGGTG CTAGACGCGA CACTGATTCA CCAATCCATC ACGGGATTAT ATGAAACTCG
 9061 GATAGATTTG TCACAGCTTG GGGGTGACGG ATCCCCCAAG AAGAAGAGGA AAGTCTCGAG
 9121 CGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC ATCGATTACA AGGATGACGA
 9181 TGACAAGTGA AGCGGCCGCA CTCCTCAGGT GCAGGCTGCC TATCAGAAGG TGGTGGCTGG
 9241 TGTGGCCAAT GCCCTGGCTC ACAAATACCA CTGAGATCTT TTTCCCTCTG CCAAAAATTA
 9301 TGGGGACATC ATGAAGCCCC TTGAGCATCT GACTTCTGGC TAATAAAGGA AATTTATTTT
 9361 CATTGCAATA GTGTGTTGGA ATTTTTTGTG TCTCTCACTC GGAAGGACAT ATGGGAGGGC
 9421 AAATCATTTA AAACATCAGA ATGAGTATTT GGTTTAGAGT TTGGCAACAT ATGCCCATAT
 9481 GCTGGCTGCC ATGAACAAAG GTTGGCTATA AAGAGGTCAT CAGTATATGA AACAGCCCCC
 9541 TGCTGTCCAT TCCTTATTCC ATAGAAAAGC CTTGACTTGA GGTTAGATTT TTTTTATATT
 9601 TTGTTTTGTG TTATTTTTTT CTTTAACATC CCTAAAATTT TCCTTACATG TTTTACTAGC
 9661 CAGATTTTTC CTCCTCTCCT GACTACTCCC AGTCATAGCT GTCCCTCTTC TCTTATGGAG
 9721 ATCCCTCGAC CTGCAGCCCA AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA
 9781 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT
 9841 GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC
 9901 AGTCGGGAAA CCTGTCGTGC CAGCGGATCC GCATCTCAAT TAGTCAGCAA CCATAGTCCC
 9961 GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA
10021 TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT
10081 CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TAACTTGTTT
10141 ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA
10201 TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC
10261 TGGATCCGCT Gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
10321 gct
//

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