质粒名称: | pSQT1601 |
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出品公司: | 爱迪基因 |
目录编号: | 53369 |
存储实验室: | Keith Joung |
载体骨架基本信息 |
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载体名称: | pCAG-CFP |
载体抗性: | 氨苄霉素 |
载体类型: | 哺乳细胞表达 |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | hCsy4-T2A-NLS-hFokI-dCas9-NLS |
启动子 | CAG |
克隆方法: | 未知 |
5' 测序引物 | pCAG-F |
3' 测序引物 | Bglob-pA-R |
文献引用方法 |
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材料和方法部分: | pSQT1601 was a gift from Keith Joung (Addgene plasmid # 53369) |
参考文献部分: | Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing. Tsai SQ, Wyvekens N, Khayter C, Foden JA, Thapar V, Reyon D, Goodwin MJ, Aryee MJ, Joung JK. Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908. 10.1038/nbt.2908 PubMed 24770325 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pSQT1601 Sequencing Result 10323 bp DNA SYN DEFINITION pSQT1601 Sequencing Result ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..10323 /organism="pSQT1601 Sequencing Result" /mol_type="other DNA" gene complement(993..1853) /label="Ampicillin" /gene="Ampicillin" CDS complement(993..1853) /label="ORF frame 2" promoter complement(1895..1923) /label="AmpR_promoter" misc_feature 2069..2356 /label="CAG_enhancer" CDS complement(2703..3311) /label="ORF frame 2" misc_feature 3646..3665 /label="pCAG_F_primer" CDS complement(3674..4201) /label="ORF frame 3" CDS 3737..9190 /label="ORF frame 2" misc_feature 4358..4378 /label="SV40 NLS" misc_feature 4364..4378 /label="NLS" misc_feature 9122..9142 /label="FLAG" misc_feature 9143..9163 /label="FLAG" misc_feature 9164..9187 /label="FLAG" misc_feature 9173..9187 /label="EK" terminator 9209..9723 /label="rb_glob_PA_terminator" promoter complement(9754..9772) /label="M13_reverse_primer" misc_feature complement(9771..9793) /label="M13_pUC_rev_primer" promoter complement(9807..9836) /label="lac_promoter" promoter 9942..10121 /label="SV40_promoter" rep_origin 9977..10054 /label="SV40_origin" misc_feature 10039..10058 /label="SV40pro_F_primer" terminator 10135..10266 /label="SV40_PA_terminator" misc_feature 10223..10242 /label="EBV_rev_primer" ORIGIN 1 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctCTTCCGCT 61 TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC 121 TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA 181 GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT 241 AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC 301 CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT 361 GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG 421 CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG 481 GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT 541 CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG 601 ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC 661 GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA 721 AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT 781 GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT 841 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA 901 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC 961 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT 1021 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA 1081 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA 1141 CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA 1201 AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA 1261 GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG 1321 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA 1381 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT 1441 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT 1501 CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA 1561 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT 1621 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA 1681 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC 1741 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG 1801 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC 1861 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 1921 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 1981 CCTGGGTCGA CATTGATTAT TGACTAGTTA TTAATAGTAA TCAATTACGG GGTCATTAGT 2041 TCATAGCCCA TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC CGCCTGGCTG 2101 ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA TAGTAACGCC 2161 AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG CCCACTTGGC 2221 AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG ACGGTAAATG 2281 GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT GGCAGTACAT 2341 CTACGTATTA GTCATCGCTA TTACCATGGT CGAGGTGAGC CCCACGTTCT GCTTCACTCT 2401 CCCCATCTCC CCCCCCTCCC CACCCCCAAT TTTGTATTTA TTTATTTTTT AATTATTTTG 2461 TGCAGCGATG GGGGCGGGGG GGGGGGGGGG GCGAGGGGCG GGGCGGGGCG AGGCGGAGAG 2521 GTGCGGCGGC AGCCAATCAG AGCGGCGCGC TCCGAAAGTT TCCTTTTATG GCGAGGCGGC 2581 GGCGGCGGCG GCCCTATAAA AAGCGAAGCG CGCGGCGGGC GGGAGTCGCT GCGCGCTGCC 2641 TTCGCCCCGT GCCCCGCTCC GCCGCCGCCT CGCGCCGCCC GCCCCGGCTC TGACTGACCG 2701 CGTTACTCCC ACAGGTGAGC GGGCGGGACG GCCCTTCTCC TCCGGGCTGT AATTAGCGCT 2761 TGGTTTAATG ACGGCTTGTT TCTTTTCTGT GGCTGCGTGA AAGCCTTGAG GGGCTCCGGG 2821 AGGGCCCTTT GTGCGGGGGG AGCGGCTCGG GGGGTGCGTG CGTGTGTGTG TGCGTGGGGA 2881 GCGCCGCGTG CGGCTCCGCG CTGCCCGGCG GCTGTGAGCG CTGCGGGCGC GGCGCGGGGC 2941 TTTGTGCGCT CCGCAGTGTG CGCGAGGGGA GCGCGGCCGG GGGCGGTGCC CCGCGGTGCG 3001 GGGGGGGCTG CGAGGGGAAC AAAGGCTGCG TGCGGGGTGT GTGCGTGGGG GGGTGAGCAG 3061 GGGGTGTGGG CGCGTCGGTC GGGCTGCAAC CCCCCCTGCA CCCCCCTCCC CGAGTTGCTG 3121 AGCACGGCCC GGCTTCGGGT GCGGGGCTCC GTACGGGGCG TGGCGCGGGG CTCGCCGTGC 3181 CGGGCGGGGG GTGGCGGCAG GTGGGGGTGC CGGGCGGGGC GGGGCCGCCT CGGGCCGGGG 3241 AGGGCTCGGG GGAGGGGCGC GGCGGCCCCC GGAGCGCCGG CGGCTGTCGA GGCGCGGCGA 3301 GCCGCAGCCA TTGCCTTTTA TGGTAATCGT GCGAGAGGGC GCAGGGACTT CCTTTGTCCC 3361 AAATCTGTGC GGAGCCGAAA TCTGGGAGGC GCCGCCGCAC CCCCTCTAGC GGGCGCGGGG 3421 CGAAGCGGTG CGGCGCCGGC AGGAAGGAAA TGGGCGGGGA GGGCCTTCGT GCGTCGCCGC 3481 GCCGCCGTCC CCTTCTCCCT CTCCAGCCTC GGGGCTGTCC GCGGGGGGAC GGCTGCCTTC 3541 GGGGGGGACG GGGCAGGGCG GGGTTCGGCT TCTGGCGTGT GACCGGCGGC TCTAGAGCCT 3601 CTGCTAACCA TGTTCATGCC TTCTTCTTTT TCCTACAGCT CCTGGGCAAC GTGCTGGTTA 3661 TTGTGCTGTC TCATCATTTT GGCAAAGAAT TCTGCAGTCG ACGGTACCGC GGGCCCGGGA 3721 TCCACCGGTC GCCACCATGG GTGATCATTA TCTGGATATT CGGCTGAGGC CTGATCCAGA 3781 GTTCCCACCT GCGCAGCTGA TGTCTGTCCT TTTTGGCAAA CTTCATCAGG CCCTGGTTGC 3841 CCAGGGCGGA GATCGGATAG GGGTAAGCTT TCCAGACCTC GACGAAAGCC GGAGCCGCCT 3901 GGGAGAACGC CTGCGGATCC ACGCTTCTGC CGACGATCTG AGAGCCTTGC TGGCAAGGCC 3961 ATGGCTTGAG GGGCTCCGGG ATCACCTGCA GTTTGGCGAA CCCGCCGTTG TTCCCCACCC 4021 AACCCCTTAT CGGCAGGTGT CTAGAGTGCA GGCCAAATCT AATCCAGAAC GGCTGCGACG 4081 GCGACTCATG CGGCGACATG ATCTTAGCGA GGAAGAGGCC CGAAAAAGAA TCCCTGATAC 4141 CGTGGCCCGC GCCCTTGACT TGCCTTTTGT CACACTGCGG TCCCAGAGTA CGGGGCAGCA 4201 TTTCAGACTT TTCATTCGAC ACGGGCCACT GCAAGTTACC GCCGAAGAAG GAGGCTTTAC 4261 TTGTTATGGA CTCTCCAAGG GAGGTTTCGT GCCCTGGTTT GAGGGCAGAG GAAGTCTGTT 4321 AACATGCGGT GACGTCGAGG AGAATCCTGG CCCAATGCCT AAGAAGAAGC GGAAGGTGAG 4381 CAGCCAACTT GTGAAGTCTG AACTCGAGGA GAAAAAATCA GAGTTGAGAC ACAAGTTGAA 4441 GTACGTGCCA CACGAATACA TCGAGCTTAT CGAGATCGCC AGAAACAGTA CCCAGGATAG 4501 GATCCTTGAG ATGAAAGTCA TGGAGTTCTT TATGAAGGTC TACGGTTATA GAGGAAAGCA 4561 CCTTGGCGGT AGCAGAAAGC CCGATGGCGC CATCTATACT GTCGGATCTC CTATCGATTA 4621 TGGGGTGATC GTGGATACCA AAGCTTACTC AGGCGGGTAC AACTTGCCCA TAGGACAAGC 4681 CGACGAGATG CAGCGGTATG TCGAAGAGAA CCAGACGCGC AACAAGCACA TCAACCCCAA 4741 TGAATGGTGG AAAGTGTACC CAAGTAGTGT GACTGAGTTC AAGTTCCTGT TTGTCTCCGG 4801 CCACTTTAAG GGCAATTATA AAGCTCAGCT CACTAGACTC AATCACATCA CAAACTGCAA 4861 CGGAGCTGTG TTGTCAGTGG AGGAGCTCCT GATTGGAGGC GAGATGATCA AAGCCGGCAC 4921 CCTTACACTG GAGGAGGTGC GGCGGAAGTT CAACAATGGA GAGATCAACT TCGGTGGCGG 4981 TGGATCCGAT AAAAAGTATT CTATTGGTTT AGCCATCGGC ACTAATTCCG TTGGATGGGC 5041 TGTCATAACC GATGAATACA AAGTACCTTC AAAGAAATTT AAGGTGTTGG GGAACACAGA 5101 CCGTCATTCG ATTAAAAAGA ATCTTATCGG TGCCCTCCTA TTCGATAGTG GCGAAACGGC 5161 AGAGGCGACT CGCCTGAAAC GAACCGCTCG GAGAAGGTAT ACACGTCGCA AGAACCGAAT 5221 ATGTTACTTA CAAGAAATTT TTAGCAATGA GATGGCCAAA GTTGACGATT CTTTCTTTCA 5281 CCGTTTGGAA GAGTCCTTCC TTGTCGAAGA GGACAAGAAA CATGAACGGC ACCCCATCTT 5341 TGGAAACATA GTAGATGAGG TGGCATATCA TGAAAAGTAC CCAACGATTT ATCACCTCAG 5401 AAAAAAGCTA GTTGACTCAA CTGATAAAGC GGACCTGAGG TTAATCTACT TGGCTCTTGC 5461 CCATATGATA AAGTTCCGTG GGCACTTTCT CATTGAGGGT GATCTAAATC CGGACAACTC 5521 GGATGTCGAC AAACTGTTCA TCCAGTTAGT ACAAACCTAT AATCAGTTGT TTGAAGAGAA 5581 CCCTATAAAT GCAAGTGGCG TGGATGCGAA GGCTATTCTT AGCGCCCGCC TCTCTAAATC 5641 CCGACGGCTA GAAAACCTGA TCGCACAATT ACCCGGAGAG AAGAAAAATG GGTTGTTCGG 5701 TAACCTTATA GCGCTCTCAC TAGGCCTGAC ACCAAATTTT AAGTCGAACT TCGACTTAGC 5761 TGAAGATGCC AAATTGCAGC TTAGTAAGGA CACGTACGAT GACGATCTCG ACAATCTACT 5821 GGCACAAATT GGAGATCAGT ATGCGGACTT ATTTTTGGCT GCCAAAAACC TTAGCGATGC 5881 AATCCTCCTA TCTGACATAC TGAGAGTTAA TACTGAGATT ACCAAGGCGC CGTTATCCGC 5941 TTCAATGATC AAAAGGTACG ATGAACATCA CCAAGACTTG ACACTTCTCA AGGCCCTAGT 6001 CCGTCAGCAA CTGCCTGAGA AATATAAGGA AATATTCTTT GATCAGTCGA AAAACGGGTA 6061 CGCAGGTTAT ATTGACGGCG GAGCGAGTCA AGAGGAATTC TACAAGTTTA TCAAACCCAT 6121 ATTAGAGAAG ATGGATGGGA CGGAAGAGTT GCTTGTAAAA CTCAATCGCG AAGATCTACT 6181 GCGAAAGCAG CGGACTTTCG ACAACGGTAG CATTCCACAT CAAATCCACT TAGGCGAATT 6241 GCATGCTATA CTTAGAAGGC AGGAGGATTT TTATCCGTTC CTCAAAGACA ATCGTGAAAA 6301 GATTGAGAAA ATCCTAACCT TTCGCATACC TTACTATGTG GGACCCCTGG CCCGAGGGAA 6361 CTCTCGGTTC GCATGGATGA CAAGAAAGTC CGAAGAAACG ATTACTCCAT GGAATTTTGA 6421 GGAAGTTGTC GATAAAGGTG CGTCAGCTCA ATCGTTCATC GAGAGGATGA CCAACTTTGA 6481 CAAGAATTTA CCGAACGAAA AAGTATTGCC TAAGCACAGT TTACTTTACG AGTATTTCAC 6541 AGTGTACAAT GAACTCACGA AAGTTAAGTA TGTCACTGAG GGCATGCGTA AACCCGCCTT 6601 TCTAAGCGGA GAACAGAAGA AAGCAATAGT AGATCTGTTA TTCAAGACCA ACCGCAAAGT 6661 GACAGTTAAG CAATTGAAAG AGGACTACTT TAAGAAAATT GAATGCTTCG ATTCTGTCGA 6721 GATCTCCGGG GTAGAAGATC GATTTAATGC GTCACTTGGT ACGTATCATG ACCTCCTAAA 6781 GATAATTAAA GATAAGGACT TCCTGGATAA CGAAGAGAAT GAAGATATCT TAGAAGATAT 6841 AGTGTTGACT CTTACCCTCT TTGAAGATCG GGAAATGATT GAGGAAAGAC TAAAAACATA 6901 CGCTCACCTG TTCGACGATA AGGTTATGAA ACAGTTAAAG AGGCGTCGCT ATACGGGCTG 6961 GGGACGATTG TCGCGGAAAC TTATCAACGG GATAAGAGAC AAGCAAAGTG GTAAAACTAT 7021 TCTCGATTTT CTAAAGAGCG ACGGCTTCGC CAATAGGAAC TTTATGCAGC TGATCCATGA 7081 TGACTCTTTA ACCTTCAAAG AGGATATACA AAAGGCACAG GTTTCCGGAC AAGGGGACTC 7141 ATTGCACGAA CATATTGCGA ATCTTGCTGG TTCGCCAGCC ATCAAAAAGG GCATACTCCA 7201 GACAGTCAAA GTAGTGGATG AGCTAGTTAA GGTCATGGGA CGTCACAAAC CGGAAAACAT 7261 TGTAATCGAG ATGGCACGCG AAAATCAAAC GACTCAGAAG GGGCAAAAAA ACAGTCGAGA 7321 GCGGATGAAG AGAATAGAAG AGGGTATTAA AGAACTGGGC AGCCAGATCT TAAAGGAGCA 7381 TCCTGTGGAA AATACCCAAT TGCAGAACGA GAAACTTTAC CTCTATTACC TACAAAATGG 7441 AAGGGACATG TATGTTGATC AGGAACTGGA CATAAACCGT TTATCTGATT ACGACGTCGA 7501 TGCCATTGTA CCCCAATCCT TTTTGAAGGA CGATTCAATC GACAATAAAG TGCTTACACG 7561 CTCGGATAAG AACCGAGGGA AAAGTGACAA TGTTCCAAGC GAGGAAGTCG TAAAGAAAAT 7621 GAAGAACTAT TGGCGGCAGC TCCTAAATGC GAAACTGATA ACGCAAAGAA AGTTCGATAA 7681 CTTAACTAAA GCTGAGAGGG GTGGCTTGTC TGAACTTGAC AAGGCCGGAT TTATTAAACG 7741 TCAGCTCGTG GAAACCCGCC AAATCACAAA GCATGTTGCA CAGATACTAG ATTCCCGAAT 7801 GAATACGAAA TACGACGAGA ACGATAAGCT GATTCGGGAA GTCAAAGTAA TCACTTTAAA 7861 GTCAAAATTG GTGTCGGACT TCAGAAAGGA TTTTCAATTC TATAAAGTTA GGGAGATAAA 7921 TAACTACCAC CATGCGCACG ACGCTTATCT TAATGCCGTC GTAGGGACCG CACTCATTAA 7981 GAAATACCCG AAGCTAGAAA GTGAGTTTGT GTATGGTGAT TACAAAGTTT ATGACGTCCG 8041 TAAGATGATC GCGAAAAGCG AACAGGAGAT AGGCAAGGCT ACAGCCAAAT ACTTCTTTTA 8101 TTCTAACATT ATGAATTTCT TTAAGACGGA AATCACTCTG GCAAACGGAG AGATACGCAA 8161 ACGACCTTTA ATTGAAACCA ATGGGGAGAC AGGTGAAATC GTATGGGATA AGGGCCGGGA 8221 CTTCGCGACG GTGAGAAAAG TTTTGTCCAT GCCCCAAGTC AACATAGTAA AGAAAACTGA 8281 GGTGCAGACC GGAGGGTTTT CAAAGGAATC GATTCTTCCA AAAAGGAATA GTGATAAGCT 8341 CATCGCTCGT AAAAAGGACT GGGACCCGAA AAAGTACGGT GGCTTCGATA GCCCTACAGT 8401 TGCCTATTCT GTCCTAGTAG TGGCAAAAGT TGAGAAGGGA AAATCCAAGA AACTGAAGTC 8461 AGTCAAAGAA TTATTGGGGA TAACGATTAT GGAGCGCTCG TCTTTTGAAA AGAACCCCAT 8521 CGACTTCCTT GAGGCGAAAG GTTACAAGGA AGTAAAAAAG GATCTCATAA TTAAACTACC 8581 AAAGTATAGT CTGTTTGAGT TAGAAAATGG CCGAAAACGG ATGTTGGCTA GCGCCGGAGA 8641 GCTTCAAAAG GGGAACGAAC TCGCACTACC GTCTAAATAC GTGAATTTCC TGTATTTAGC 8701 GTCCCATTAC GAGAAGTTGA AAGGTTCACC TGAAGATAAC GAACAGAAGC AACTTTTTGT 8761 TGAGCAGCAC AAACATTATC TCGACGAAAT CATAGAGCAA ATTTCGGAAT TCAGTAAGAG 8821 AGTCATCCTA GCTGATGCCA ATCTGGACAA AGTATTAAGC GCATACAACA AGCACAGGGA 8881 TAAACCCATA CGTGAGCAGG CGGAAAATAT TATCCATTTG TTTACTCTTA CCAACCTCGG 8941 CGCTCCAGCC GCATTCAAGT ATTTTGACAC AACGATAGAT CGCAAACGAT ACACTTCTAC 9001 CAAGGAGGTG CTAGACGCGA CACTGATTCA CCAATCCATC ACGGGATTAT ATGAAACTCG 9061 GATAGATTTG TCACAGCTTG GGGGTGACGG ATCCCCCAAG AAGAAGAGGA AAGTCTCGAG 9121 CGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC ATCGATTACA AGGATGACGA 9181 TGACAAGTGA AGCGGCCGCA CTCCTCAGGT GCAGGCTGCC TATCAGAAGG TGGTGGCTGG 9241 TGTGGCCAAT GCCCTGGCTC ACAAATACCA CTGAGATCTT TTTCCCTCTG CCAAAAATTA 9301 TGGGGACATC ATGAAGCCCC TTGAGCATCT GACTTCTGGC TAATAAAGGA AATTTATTTT 9361 CATTGCAATA GTGTGTTGGA ATTTTTTGTG TCTCTCACTC GGAAGGACAT ATGGGAGGGC 9421 AAATCATTTA AAACATCAGA ATGAGTATTT GGTTTAGAGT TTGGCAACAT ATGCCCATAT 9481 GCTGGCTGCC ATGAACAAAG GTTGGCTATA AAGAGGTCAT CAGTATATGA AACAGCCCCC 9541 TGCTGTCCAT TCCTTATTCC ATAGAAAAGC CTTGACTTGA GGTTAGATTT TTTTTATATT 9601 TTGTTTTGTG TTATTTTTTT CTTTAACATC CCTAAAATTT TCCTTACATG TTTTACTAGC 9661 CAGATTTTTC CTCCTCTCCT GACTACTCCC AGTCATAGCT GTCCCTCTTC TCTTATGGAG 9721 ATCCCTCGAC CTGCAGCCCA AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA 9781 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT 9841 GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC 9901 AGTCGGGAAA CCTGTCGTGC CAGCGGATCC GCATCTCAAT TAGTCAGCAA CCATAGTCCC 9961 GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA 10021 TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT 10081 CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TAACTTGTTT 10141 ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA 10201 TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC 10261 TGGATCCGCT Gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 10321 gct //
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