| 质粒名称: | pUB_smFLAG_KDM5B_MS2 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 81084 |
| 存储实验室: | Tim Stasevich |
|
载体骨架基本信息 |
|
| 载体名称: | pHAGE-UbiC |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | 13642 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | smFLAG_KDM5B_24xMS2 |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pUB_smFLAG_KDM5B_MS2 was a gift from Tim Stasevich (Addgene plasmid # 81084 ; http://n2t.net/addgene:81084 ; RRID:Addgene_81084) |
| 参考文献部分: | Real-time quantification of single RNA translation dynamics in living cells. Morisaki T, Lyon K, DeLuca KF, DeLuca JG, English BP, Zhang Z, Lavis LD, Grimm JB, Viswanathan S, Looger LL, Lionnet T, Stasevich TJ. Science. 2016 Jun 17;352(6292):1425-9. doi: 10.1126/science.aaf0899. Epub 2016 May 5. 10.1126/science.aaf0899 PubMed 27313040 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pUB_smFLAG_KDM5B_MS2 13637 bp ds-DNA circular SYN 03-SEP-2020
DEFINITION Translation reporter: Encodes KDM5B (1544 aa) fused to smFLAG
(Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG). .
ACCESSION .
VERSION .
KEYWORDS pUB_smFLAG_KDM5B_MS2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13637)
AUTHORS Morisaki T, Lyon K, DeLuca KF, DeLuca JG, English BP, Zhang Z, Lavis
LD, Grimm JB, Viswanathan S, Looger LL, Lionnet T, Stasevich TJ
TITLE Real-time quantification of single RNA translation dynamics in
living cells.
JOURNAL Science. 2016 Jun 17;352(6292):1425-9. doi: 10.1126/science.aaf0899.
Epub 2016 May 5.
PUBMED 27313040
REFERENCE 2 (bases 1 to 13637)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 3, 2020 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..13637
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2058..2175
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2218..2616
/label=UbC promoter
/note="human ubiquitin C promoter"
regulatory 2622..2631
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 2631..2654
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 2658..2681
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 2685..2708
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 3213..3236
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 3243..3266
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 3279..3302
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 3309..3332
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 3525..3548
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 3552..3575
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 3579..3602
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
misc_feature 10027..10615
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(10080..10100)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(10498..10509)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 10690..10923
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
primer_bind complement(11042..11060)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 11128..11232
/gene="bla"
/label=AmpR promoter
CDS 11233..12093
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(11451..11470)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 12264..12852
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 12753..12772
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 13006..13023
/label=L4440
/note="L4440 vector, forward primer"
primer_bind 13115..13134
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
protein_bind 13263..13284
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 13299..13329
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 13337..13353
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 13342..13364
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 13361..13377
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 13361..13377
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 13427..13446
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtggc
2221 ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca
2281 cgtcagacga agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg
2341 cccgctgctc ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac
2401 gggacttggg tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa
2461 agtagtccct tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat
2521 tatataagga cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc
2581 ggttcttgtt tgtggatcgc tgtgatcgtc acttggcggc cgccaccatg gactacaagg
2641 acgacgacga caaaggtgac tacaaagatg atgacgataa aggcgactat aaggacgatg
2701 acgacaaggg cggaaactca ctgatcaagg aaaacatgcg gatgaaggtg gtgatggagg
2761 gctccgtgaa tggtcaccag ttcaagtgca ccggagaggg agagggaaac ccgtacatgg
2821 gaactcagac catgcgcatt aaggtcatcg aaggaggtcc gctgccgttc gctttcgata
2881 tcctggccac ttcgttcgga ggagggtcgc gcacgttcat caagtacccg aagggaatcc
2941 cggacttctt taagcagtca ttcccggaag gattcacttg ggaacgggtg acccggtatg
3001 aagatggagg tgtggtgact gtcatgcaag atacttcgct ggaggatggg tgcctcgtgt
3061 accacgtcca agtccgcgga gtgaatttcc cgtccaacgg accagtgatg cagaaaaaga
3121 cgaagggttg ggaacctaat actgaaatga tgtaccccgc agacggaggg ctgaggggct
3181 acacccacat ggcgctgaag gtcgacggag gagattacaa ggatgacgac gataagcaac
3241 aagattacaa agacgatgat gacaagggcc agcagggcga ctacaaggac gacgacgaca
3301 agcagcagga ctacaaagat gacgatgata aaggaggagg acatctgtcc tgttcgttcg
3361 tgaccaccta cagatcaaag aaaaccgtgg gaaacatcaa gatgccgggc attcatgccg
3421 tcgaccaccg cctggagcgg ctcgaagaat cagacaatga gatgttcgtc gtgcaaagag
3481 aacatgccgt ggccaagttc gcgggactgg gaggcggtgg aggcgattac aaagacgatg
3541 atgacaaggg tgactataaa gacgacgatg acaaagggga ttacaaggat gatgatgata
3601 agggaggcgg tggatcaggt ggaggaggtt cactgcaggc gatcgccatg gaggcggcca
3661 ccacactgca cccaggcccg cgcccggcgc tgcccctcgg gggcccgggc ccgctgggcg
3721 agttcctgcc tccacccgag tgcccggtct tcgaacccag ctgggaagag ttcgcggacc
3781 ccttcgcttt catccacaag atccggccca tagccgagca gactggcatc tgtaaggtgc
3841 ggccgccgcc ggattggcag ccaccatttg catgtgatgt tgataaactt cattttacgc
3901 cacgtatcca gagactgaat gaattggagg cccaaactcg tgtaaaattg aatttcttgg
3961 accagattgc aaagtactgg gagttacagg gaagtactct gaaaattcca catgtggaga
4021 ggaagatctt ggacttattt cagcttaata agttagttgc agaagaaggt ggatttgcag
4081 ttgtttgcaa ggatagaaaa tggaccaaaa ttgctaccaa gatggggttt gctcctggca
4141 aagcagtggg ctcacatatc agagggcatt atgaacgaat tctcaacccc tacaacttat
4201 tcctgtccgg agacagccta aggtgtttgc agaagccaaa cctgaccaca gacactaagg
4261 acaaggagta caaaccccat gatattcccc agaggcagtc tgtgcagcct tcggaaacgt
4321 gccccccagc ccgacgagca aaacgcatga gagcagaggc catgaatatt aaaatagaac
4381 ccgaggagac aacagaagcc agaactcata atctgagacg tcgaatgggt tgtccaactc
4441 caaaatgtga aaatgagaaa gaaatgaaga gtagcatcaa gcaagaacct attgagagga
4501 aagattatat tgtagaaaat gagaaggaaa agcccaagag tcgatctaaa aaagccacca
4561 atgctgtgga cctgtatgtc tgtcttttat gtggcagtgg caatgatgaa gaccggctac
4621 tgttgtgtga tggctgtgat gacagttacc ataccttttg cttgatccca cctctccatg
4681 atgttcccaa gggagactgg aggtgtccta agtgtttggc tcaggaatgt agtaagccac
4741 aagaagcatt tggctttgaa caagcagcca gggactatac cctccgtact tttggggaaa
4801 tggcagatgc gttcaaatct gattacttca acatgccagt ccatatggtc cccacagagc
4861 ttgttgagaa agaattttgg agactagtaa gcactattga ggaggatgtc acagtggaat
4921 atggagctga cattgcctca aaggaatttg gcagtggctt tcctgtccga gatgggaaaa
4981 tcaaactctc acctgaggaa gaggagtatc ttgatagtgg ctggaatttg aacaacatgc
5041 cagtgatgga gcagtctgtc cttgcacata ttactgctga tatatgtggc atgaaacttc
5101 cttggttgta tgtgggaatg tgcttttctt cattctgttg gcacattgaa gaccactgga
5161 gctattcaat taactacttg cactggggtg agccaaaaac ctggtatgga gtcccagggt
5221 atgctgctga gcagctagaa aatgtaatga agaaactagc tccagaactc tttgtgtccc
5281 agccggatct cctccatcag cttgtgacca tcatgaaccc caataccctg atgactcatg
5341 aagtgcctgt ttaccgaact aatcagtgtg ctggggagtt tgtgattaca tttccaagag
5401 cctaccacag tggttttaac cagggtttta attttgctga ggctgttaac ttctgcactg
5461 ttgattggct gccattaggc cgacagtgtg tggagcatta tcgcttgctt catcgatatt
5521 gtgtgttttc ccacgatgag atgatctgca agatggcttc caaggctgat gtattagatg
5581 ttgtagtggc ttcaactgtt cagaaagaca tggccattat gattgaggat gagaaagctt
5641 taagagaaac tgtccgtaaa ttgggagtga ttgattcgga aagaatggat tttgagctgt
5701 tgccagatga tgaacgtcag tgtgtaaaat gcaaaactac atgcttcatg tctgccatct
5761 cctgttcttg taaacctggc cttcttgttt gcctgcatca tgtaaaagaa ttgtgttcct
5821 gtcctcctta taaatataaa ttgcggtata ggtacacgct ggatgatctc taccctatga
5881 tgaatgcatt gaagcttcga gcagaatctt acaacgaatg ggccttgaat gtgaatgaag
5941 ctttggaggc aaagatcaac aagaagaaaa gccttgtcag cttcaaggct ttaattgaag
6001 aatctgaaat gaagaaattc ccagacaatg atcttttgcg acaccttcgc ctagtcacac
6061 aggatgcaga gaagtgtgcc tctgttgcgc agcagttgct taatggcaaa aggcaaacta
6121 gatatcgatc tggtggaggg aaatcccaaa atcagttgac agtgaatgag ctccggcagt
6181 ttgtaacaca gctgtatgct cttccatgtg tcctcagtca gacaccatta ctaaaggatc
6241 tcttgaatcg tgtagaagat tttcaacagc atagtcagaa actactctct gaggaaacgc
6301 ctagtgctgc ggagctgcag gacttgctag atgtcagctt tgaatttgat gttgaacttc
6361 cacagcttgc tgagatgcgt atccgtttgg aacaagcccg ttggctagaa gaggtgcagc
6421 aagcttgcct agaccccagc tcccttactt tagatgatat gagacgtctc atagacctag
6481 gggtagggct ggccccgtat tcagcagtgg agaaagctat ggcccggctg caggaactgc
6541 tcacagtgtc agagcactgg gacgacaaag ccaagagtct cctcaaggcc aggccacgac
6601 attcattgaa tagccttgct acggcagtaa aggaaatcga agagatccct gcatatctgc
6661 ccaatggtgc ggctctgaaa gactcagtgc agagagccag agactggctt caggatgtag
6721 agggcctgca ggctggagga cgtgtgccag tgttagacac actcatagaa cttgttacac
6781 gaggccgatc tatcccggta catctgaatt ctttgccaag actggaaacc ctagtagctg
6841 aggttcaggc ttggaaagaa tgtgctgtta atacattctt gactgagaat tctccatatt
6901 ctctcttaga ggtgctgtgt cctcgatgtg atattggcct tttgggattg aaaaggaagc
6961 agagaaagtt aaaggagccc ttgccaaatg gaaagaaaaa aagcaccaaa ttagagagtc
7021 tgagtgacct ggagagagct ttaactgaaa gcaaggagac tgcttcagct atggcaactc
7081 ttggggaagc tcgcctaagg gaaatggaag ccttgcagtc tctcagactc gccaatgaag
7141 ggaaattgct gtcgcctctc caagatgtgg atataaaaat ctgcctatgt cagaaggccc
7201 cagctgcccc tatgattcaa tgtgaactct gcagggatgc tttccacacc agttgtgtgg
7261 cggtacccag tatttcacag ggcctgcgaa tctggctttg tccccattgt cggaggtcag
7321 agaaacctcc attagagaaa attctgcccc tgctcgcctc ccttcagcgt atccgagttc
7381 gccttcctga gggagatgca cttcgatata tgattgaaag aaccgtgaac tggcagcaca
7441 gagcccagca actgctttcg tcagggaatc ttaaatttgt gcaagatcga gtgggctcag
7501 gactgttata tagcagatgg caagcctcag caggacaggt gtcagacaca aacaaggtat
7561 ctcaacctcc tggcacaaca tcattttctt tgcctgatga ctgggacaac agaacctcat
7621 atttgcactc ccccttctca actggacgaa gttgtatccc cctccatggt gttagtccag
7681 aagtgaatga actattgatg gaagcccagc tgctccaggt atcccttcct gaaattcagg
7741 aactttacca gactttactt gcaaagccaa gccctgctca gcagactgac cgaagctcac
7801 cagtgagacc cagcagtgag aagaatgact gttgccgagg gaagcgagat ggaattaaca
7861 gtcttgagag aaaactgaag agacgcctgg aaagagaggg cctctccagt gagcggtggg
7921 aacgagttaa gaaaatgcgg acccccaaaa agaagaaaat caaactgagc caccccaagg
7981 acatgaacaa tttcaagtta gagagagagc gtagctatga attagttcgt tctgctgaaa
8041 ctcattccct gccctcagac acatcctatt ccgaacagga agactctgag gatgaagatg
8101 ccatctgccc agctgtgagc tgcctgcagc cagaaggaga tgaggtggac tgggtccagt
8161 gtgatggcag ctgcaatcag tggtttcatc aggtctgtgt tggtgtctcc ccagagatgg
8221 cagagaaaga agactacatc tgtgtgcgct gtactgtgaa ggacgcacca agccgaaagg
8281 tttaaacgct agctaggcgg actgttactg agctgcgttt tacacccttt ctttgacaaa
8341 acctaacttg cgcagaaaaa aaaaaaataa gagacaacat tggcatggct ttgttttttt
8401 aaattttttt taaagttttt tttttttttt tttttttttt aagttttttt gttttgtttt
8461 ggcgcttttg actcaggatt taaaaactgg aacggtgaag gcgacagcag ttggttggag
8521 caaacatccc ccaaagttct acaaatgtgg ctgaggactt tgtacattgt tttgtttttt
8581 ttttttttgg ttttgtcttt ttttaatagt cattccaagt atccatgaaa taagtggtta
8641 caggaagtcc ctcaccctcc caaaagccac ccccactcct aagaggagga tggtcgcgtc
8701 catgccctga gtccaccccg gggaaggtga caaccggtaa cctacaaacg ggtggaggat
8761 caccccaccc gacacttcac aatcaagggg tacaatacac aagggtggag gaacacccca
8821 ccctccagac acattacaca gaaatccaat caaacagaag caccatcagg gcttctgcta
8881 ccaaatttat ctcaaaaaac tacaacaagg aatcaccatc agggattccc tgtgcaatat
8941 acgtcaaacg agggccacga cgggaggacg atcacgcctc ccgaatatcg gcatgtctgg
9001 ctttcgaatt cagtgcgtgg agcatcagcc cacgcagcca atcagagtcg aatacaagtc
9061 gactttcgcg aagagcatca gccttcgcgc cattcttaca caaaccacac tctcccctac
9121 aggaacagca tcagcgttcc tgcccagtac ccaactcaag aaaatttatg tccccatgca
9181 gcatcagcgc atgggcccca agaatacatc cccaacaaaa tcacatccga gcaccaacag
9241 ggctcggagt gttgtttctt gtccaactgg acaaaccctc catggaccat caggccatgg
9301 actctcacca acaagacaaa aactactctt ctcgaagcag catcagcgct tcgaaacact
9361 cgagcataca ttgtgcctat ttcttgggtg gacgatcacg ccacccatgc tctcacgaat
9421 ttcaaaacac ggacaaggac gagcaccacc agggctcgtc gttccacgtc caatacgatt
9481 acttaccttt cgggatcacg atcacggatc ccgcagctac atcacttcca ctcaggacat
9541 tcaagcatgc acgatcacgg catgctccac aagtctcaac cacagaaact accaaatggg
9601 ttcagcacca gcgaacccac tcctacctca aacctcttcc cacaaaactg gcaagcagga
9661 tcaccgcttg cccattccaa cataccaaat caaaaacaat tactggtaca gcatcagcgt
9721 accagcccac atctctcact actatcaaaa accaaaccgt tcagcaacag cgaacggtac
9781 acacggaaaa atcaactggt ttacaaatac gaaagacgat cacgctttcg tccagcgcaa
9841 actattacga aaaacatccg acgggaagag caacagcctt cccgcggcgg aaaacctcac
9901 aaaaacacga caaacggatg cacgaacacg gcatccgccg acaacccaca aacttacaac
9961 caggcaaacg gtgcaggatc accgcaccgt acatcaaaca cctcagatct catatcgata
10021 gatcctaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
10081 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
10141 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
10201 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
10261 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
10321 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
10381 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
10441 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
10501 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
10561 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgag
10621 atcctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
10681 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
10741 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
10801 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
10861 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
10921 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
10981 cagagagtga gaggcccggg ttaattaagg aaagggctag atcattcttg aagacgaaag
11041 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
11101 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
11161 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
11221 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
11281 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
11341 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
11401 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
11461 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
11521 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
11581 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
11641 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
11701 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
11761 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
11821 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
11881 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
11941 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
12001 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
12061 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
12121 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
12181 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
12241 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
12301 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
12361 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
12421 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
12481 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
12541 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
12601 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
12661 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
12721 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
12781 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
12841 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
12901 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
12961 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
13021 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
13081 taatgcagca agctcatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
13141 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
13201 aaaagctccc cgtggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
13261 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
13321 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg
13381 attacgaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
13441 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
13501 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
13561 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
13621 actacaaact tagtagt
//
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