| 质粒名称: | pcDNA3β-FLAG-CBP-HA |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 32908 |
| 存储实验室: | Tso-Pang Yao |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3 |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5400 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | CBP,acetyltransferase CBP,p300/CBP,CREB binding protein |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: |
FLAG（插入N端子）
HA（插入件上的C端子）
|
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | CMV-Fwd |
| 3' 测序引物 | BGH-Rev |
|
文献引用方法 |
|
| 材料和方法部分: | pcDNA3β-FLAG-CBP-HA was a gift from Tso-Pang Yao (Addgene plasmid # 32908 ; http://n2t.net/addgene:32908 ; RRID:Addgene_32908) |
| 参考文献部分: | Regulation of MEF2 by histone deacetylase 4- and SIRT1 deacetylase-mediated lysine modifications. Zhao X, Sternsdorf T, Bolger TA, Evans RM, Yao TP. Mol Cell Biol. 2005 Oct;25(19):8456-64. 10.1128/MCB.25.19.8456-8464.2005 PubMed 16166628 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pcDNA3-beta-FLAG-CBP-HA 13414 bp ds-DNA circular SYN 12-MAR-2019
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pcDNA3-beta-FLAG-CBP-HA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13414)
AUTHORS Zhao X, Sternsdorf T, Bolger TA, Evans RM, Yao TP
TITLE Regulation of MEF2 by histone deacetylase 4- and SIRT1
deacetylase-mediated lysine modifications.
JOURNAL Mol Cell Biol. 2005 Oct;25(19):8456-64.
PUBMED 16166628
REFERENCE 2 (bases 1 to 13414)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Mar 12, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..13414
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(11..28)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(182..770)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(262..281)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(941..1801)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1564..1583
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1802..1906)
/gene="bla"
/label=AmpR promoter
primer_bind complement(1981..2000)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 2172..2551
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2552..2755
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2706..2726
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 2800..2819
/label=T7
/note="T7 promoter, forward primer"
promoter 2800..2818
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
intron 2855..3427
/label=-beta-globin intron
/note="intron from rabbit beta-globin gene"
CDS 3473..3496
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
regulatory 8363..8372
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 10838..10864
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
promoter complement(10904..10922)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(10905..10922)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(10942..10959)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 10948..11172
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 11218..11646
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(11305..11324)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 11515..11536
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(11655..11675)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 11660..11989
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 11840..11975
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 11902..11921
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 12056..12850
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(12110..12129)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 12720..12739
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 13024..13145
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(13061..13080)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 13115..13134
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(13194..13210)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(13194..13210)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(13207..13229)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 13218..13234
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(13242..13272)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 13287..13308
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
ORIGIN
1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
1921 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag
1981 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
2041 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
2101 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
2161 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
2221 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
2281 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
2341 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
2401 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
2461 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
2521 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
2581 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
2641 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
2701 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg
2761 gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga
2821 gacccaagct agagtcgatc ctgagaactt cagggtgagt ttggggaccc ttgattgttc
2881 tttctttttc gctattgtaa aattcatgtt atatggaggg ggcaaagttt tcagggtgtt
2941 gtttagaatg ggaagatgtc ccttgtatca ccatggaccc tcatgataat tttgtttctt
3001 tcactttcta ctctgttgac aaccattgtc tcctcttatt ttcttttcat tttctgtaac
3061 tttttcgtta aactttagct tgcatttgta acgaattttt aaattcactt ttgtttattt
3121 gtcagattgt aagtactttc tctaatcact tttttttcaa ggcaatcagg gtatattata
3181 ttgtacttca gcacagtttt agagaacaat tgttataatt aaatgataag gtagaatatt
3241 tctgcatata aattctggct ggcgtggaaa tattcttatt ggtagaaaca actacatcct
3301 ggtcatcatc ctgcctttct ctttatggtt acaatgatat acactgtttg agatgaggat
3361 aaaatactct gagtccaaac cgggcccctc tgctaaccat gttcatgcct tcttcttttt
3421 cctacagctc ctgggcaacg tgctggtttg tgctgtcgac cccaagctta tggactacaa
3481 ggacgacgac gacaaagccg agaacttgct ggacggaccg cccaacccca aacgagccaa
3541 actcagctcg cccggcttct ccgcgaatga caacacagat tttggatcat tgtttgactt
3601 ggaaaatgac cttcctgatg agctgatccc caatggagaa ttaagccttt taaacagtgg
3661 gaaccttgtt ccagatgctg cgtccaaaca taaacaactg tcagagcttc ttagaggagg
3721 cagcggctct agcatcaacc cagggatagg caatgtgagt gccagcagcc ctgtgcaaca
3781 gggccttggt ggccaggctc aggggcagcc gaacagtaca aacatggcca gcttaggtgc
3841 catgggcaag agccctctga accaaggaga ctcatcaaca cccaacctgc ccaaacaggc
3901 agccagcacc tctgggccca ctccccctgc ctcccaagca ctgaatccac aagcacaaaa
3961 gcaagtaggg ctggtgacca gtagtcctgc cacatcacag actggacctg ggatctgcat
4021 gaatgctaac ttcaaccaga cccacccagg ccttctcaat agtaactctg gccatagctt
4081 aatgaatcag gctcaacaag ggcaagctca agtcatgaat ggatctcttg gggctgctgg
4141 aagaggaagg ggagctggaa tgccctaccc tgctccagcc atgcaggggg ccacaagcag
4201 tgtgctggcg gagaccttga cacaggtttc cccacaaatg gctggccatg ctggactaaa
4261 tacagcacag gcaggaggca tgaccaagat gggaatgact ggtaccacaa gtccatttgg
4321 acaacccttt agtcaaactg gagggcagca gatgggagcc actggagtta acccccagtt
4381 agccagcaaa cagagcatgg tcaatagttt acctgctttt cctacagata tcaagaatac
4441 ttcagtcacc actgtgccaa atatgtccca gttgcaaaca tcagtgggaa ttgtacccac
4501 acaagcaatt gcaacaggcc ccacagcaga ccctgaaaaa cgcaaactga tacagcagca
4561 gctggttcta ctgcttcatg cccacaaatg tcagagacga gagcaagcaa atggagaggt
4621 tcgagcctgt tctctcccac actgtcgaac catgaaaaac gttttgaatc acatgacaca
4681 ttgtcaggct gggaaagcct gccaagttgc ccattgtgca tcttcacgac aaatcatctc
4741 tcattggaag aactgcacac gacatgactg tcctgtttgc ctccctttga aaaatgccag
4801 tgacaagcga aaccaacaaa ccatcctggg atctccagct agtggaattc aaaacacaat
4861 tggttctgtt ggtgcagggc aacagaatgc cacttcctta agtaacccaa atcccataga
4921 ccccagttcc atgcagcggg cctatgctgc tctaggactc ccctacatga accagcctca
4981 gacgcagctg cagcctcagg ttcctggcca gcaaccagca cagcctccag cccaccagca
5041 gatgaggact ctcaatgccc taggaaacaa ccccatgagt atcccagcag gaggaataac
5101 aacagatcaa cagccaccaa acttgatttc agaatcagct cttccaactt ccttgggggc
5161 taccaatcca ctgatgaatg atggttcaaa ctctggtaac attggaagcc tcagcacgat
5221 acctacagca gcgcctcctt ccagcactgg tgttcgaaaa ggctggcatg aacatgtgac
5281 tcaggaccta cggagtcatc tagtccataa actcgttcaa gccatcttcc caactccaga
5341 ccctgcagct ctgaaagatc gccgcatgga gaacctggtt gcctatgcta agaaagtgga
5401 gggagacatg tatgagtctg ctaatagcag ggatgaatac tatcatttat tagcagagaa
5461 aatctataaa atacaaaaag aactagaaga aaagcggagg tcacgtttac ataagcaagg
5521 catcctgggt aaccagccag ctttaccagc ttctggggct cagccccctg tgattccacc
5581 agcccagtct gtaagacctc caaatgggcc cctgcctttg ccagtgaatc gcatgcaggt
5641 ttctcaaggg atgaattcat ttaacccaat gtccctggga aacgtccagt tgccacaggc
5701 acccatggga cctcgtgcag cctcccctat gaaccactct gtgcagatga acagcatggc
5761 ctcagttccg ggtatggcca tttctccttc acggatgcct cagcctccaa atatgatggg
5821 cactcatgcc aacaacatta tggcccaggc acctactcag aaccagtttc tgccacagaa
5881 ccagtttcca tcatccagtg gggcaatgag tgtgaacagt gtgggcatgg ggcaaccagc
5941 agcccaggca ggtgtttcac agggtcaggt acctggagct gctctcccta accctctgaa
6001 catgctggca ccccaggcca gccagctgcc ttgcccacca gtgacacagt caccattgca
6061 cccgactcca cctcctgctt ccacagctgc tggcatgccc tctctccaac atccaacggc
6121 accaggaatg acccctcctc agccagcagc tcccactcag ccatctactc ctgtgtcatc
6181 tgggcagact cctaccccaa ctcctggctc agtgcccagc gctgcccaaa cacagagtac
6241 ccctacagtc caggcagcag cacaggctca ggtgactcca cagcctcaga ccccagtgca
6301 gccaccatct gtggctactc ctcagtcatc acagcagcaa ccaacgcctg tgcatactca
6361 gcctcctggc acaccgcttt ctcaggcagc agccagcatt gataatagag tccctactcc
6421 ctcctctgtg accagtgctg aaaccagttc ccagcagcca ggacccgatg tgcccatgct
6481 ggaaatgaag acagaggtgc agacagatga tgctgagcct gaacctactg aatccaaggg
6541 ggaacctcgg tctgagatga tggaagagga tttacaaggt tcttcccaag taaaagaaga
6601 gacagatacg acagagcaga agtcagagcc aatggaagta gaagaaaaga aacctgaagt
6661 aaaagtggaa gctaaagagg aagaagagaa cagttcgaac gacacagcct cacaatcaac
6721 atctccttcc cagccacgca aaaaaatctt taaacccgag gagctacgcc aggcacttat
6781 gccaactcta gaagcactct atcgacagga cccagagtct ttgccttttc gtcagcctgt
6841 agatcctcag ctcctaggaa tcccagatta ttttgatata gtgaagaatc ctatggacct
6901 ttctaccatc aaacgaaagc tggacacagg gcaatatcaa gaaccctggc agtatgtgga
6961 tgatgtctgg cttatgttca acaatgcgtg gctatataat cgtaaaacgt cccgtgtata
7021 taaattttgc agtaaacttg cagaggtctt tgaacaagaa attgaccctg tcatgcagtc
7081 tcttggatat tgctgtggac gaaagtatga gttctcccca cagactttgt gctgttacgg
7141 aaagcagctg tgtacaattc ctcgtgatgc agcctactac agctatcaga ataggtatca
7201 tttctgtgag aagtgtttca cagagatcca gggcgagaat gtgaccctgg gtgacgaccc
7261 ttcccaacct cagacgacaa tttccaagga tcaatttgaa aagaagaaaa atgatacctt
7321 agatcctgaa ccttttgttg actgcaaaga gtgtggccgg aagatgcatc agatttgtgt
7381 tctacactat gacatcattt ggccttcagg ttttgtgtgt gacaactgtt tgaagaaaac
7441 tggcagacct cggaaagaaa acaaattcag tgctaagagg ctgcagacca cacgattggg
7501 aaaccactta gaagacagag tgaataagtt tttgcggcgc cagaatcacc ctgaagctgg
7561 ggaggttttt gtcagagtgg tggccagctc agacaagact gtggaggtca agccgggaat
7621 gaagtcaagg tttgtggatt ctggagagat gtcggaatct ttcccatatc gtaccaaagc
7681 actctttgct tttgaggaga tcgatggagt cgatgtgtgc ttttttggga tgcatgtgca
7741 agaatacggc tctgattgcc ccccaccaaa tacaaggcgt gtatacatat cttatctgga
7801 cagtattcat ttcttccggc cccgctgcct ccggacagct gtttaccatg agatcctcat
7861 cggatatctc gagtatgtga agaaattggg gtatgtgaca ggacatattt gggcctgtcc
7921 cccaagtgaa ggagatgact atatctttca ttgccacccc cctgaccaga aaatccccaa
7981 accaaaacga ctacaggagt ggtacaagaa gatgctggac aaggcgtttg cagagaggat
8041 cattaacgac tataaggaca tcttcaaaca agcgaacgaa gacaggctca cgagtgccaa
8101 ggagttgccc tattttgaag gagatttctg gcctaatgtg ttggaagaaa gcattaagga
8161 actagaacaa gaagaagaag aaaggaaaaa agaagagagt actgcagcga gtgagactcc
8221 tgagggcagt cagggtgaca gcaaaaatgc gaagaaaaag aacaacaaga agaccaacaa
8281 aaacaaaagc agcattagcc gcgccaacaa gaagaagccc agcatgccca atgtttccaa
8341 cgacctgtcg cagaagctgt atgccaccat ggagaagcac aaggaggtat tctttgtgat
8401 tcatctgcat gctgggcctg ttatcagcac tcagcccccc atcgtggacc ctgatcctct
8461 gcttagctgt gacctcatgg atgggcgaga tgccttcctc accctggcca gagacaagca
8521 ctgggaattc tcttccttac gccgctccaa atggtccact ctgtgcatgc tggtggagct
8581 gcacacacag ggccaggacc gctttgttta tacctgcaat gagtgcaaac accatgtgga
8641 aacacgctgg cactgcactg tgtgtgagga ctatgacctt tgtatcaatt gctacaacac
8701 aaagagccac acccataaga tggtgaagtg ggggctaggc ctagatgatg agggcagcag
8761 tcagggtgag ccacagtcca agagccccca ggaatcccgg cgtctcagca tccagcgctg
8821 catccagtcc ctggtgcatg cctgccagtg tcgcaatgcc aactgctcac tgccgtcttg
8881 ccagaagatg aagcgagtcg tgcagcacac caagggctgc aagcgcaaga ctaatggagg
8941 atgcccagtg tgcaagcagc tcattgctct ttgctgctac cacgccaaac actgccaaga
9001 aaataaatgc cctgtgccct tctgcctcaa catcaaacat aagctccgcc agcagcagat
9061 ccagcatcgc ctgcagcagg ctcagctcat gcgccggcga atggcaacca tgaacacccg
9121 caatgtgcct cagcagagtt tgccttctcc tacctcagca ccacccggga ctcctacaca
9181 gcagcccagc acaccccaaa caccacagcc cccagcccag cctcagcctt cacctgttaa
9241 catgtcacca gctggcttcc ctaatgtagc ccggactcag cccccaacaa tagtgtctgc
9301 tgggaagcct accaaccagg tgccagctcc cccaccccct gcccagcccc cacctgcagc
9361 agtagaagca gcccggcaaa ttgaacgtga ggcccagcag cagcagcacc tataccgagc
9421 aaacatcaac aatggcatgc ccccaggacg tgcaggtatg gggaccccag gaagccaaat
9481 gactcctgtg ggcctgaatg tgccccgtcc caaccaagtc agtgggcctg tcatgtctag
9541 tatgccacct gggcagtggc agcaggcacc catccctcag cagcagccga tgccaggcat
9601 gcccaggcct gtaatgtcca tgcaggccca ggcagcagtg gctgggccac ggatgcccaa
9661 tgtgcagcca ccaaggagca tctcgccaag tgccctgcaa gacctgctac ggaccctaaa
9721 gtcacccagc tctcctcagc agcagcagca ggtgctgaac atccttaaat caaacccaca
9781 gctaatggca gctttcatca aacagcgcac agccaagtat gtggccaatc agcctggcat
9841 gcagccccag cccggacttc aatcccagcc tggtatgcag ccccagcctg gcatgcacca
9901 gcagcctagt ttgcaaaacc tgaacgcaat gcaagctggt gtgccacggc ctggtgtgcc
9961 tccaccacaa ccagcaatgg gaggcctgaa tccccaggga caagctctga acatcatgaa
10021 cccaggacac aaccccaaca tgacaaacat gaatccacag taccgagaaa tggtgaggag
10081 acagctgcta cagcaccagc agcagcagca gcaacagcag cagcagcagc agcaacaaca
10141 aaatagtgcc agcttggccg ggggcatggc gggacacagc cagttccagc agccacaagg
10201 acctggaggt tatgccccag ccatgcagca gcaacgcatg caacagcacc tccccatcca
10261 gggcagctcc atgggccaga tggctgctcc aatgggacaa cttggccaga tggggcagcc
10321 tgggctaggg gcagacagca cccctaatat ccagcaggcc ctgcagcaac ggattctgca
10381 gcagcagcag atgaagcaac aaattgggtc accaggccag ccgaacccca tgagccccca
10441 gcagcacatg ctctcaggac agccacaggc ctcacatctc cctggccagc agatcgccac
10501 atcccttagt aaccaggtgc gatctccagc ccctgtgcag tctccacggc cccaatccca
10561 acctccacat tccagcccgt caccacggat acaaccccag ccttcaccac accatgtttc
10621 accccagact ggttcccctc accctggact cgcagtcacc atggccagct ccatggatca
10681 gggacacctg gggaaccctg aacagagtgc aatgctcccc cagctgaata cccccaacag
10741 gagcgcactg tccagtgaac tgtccctggt tggtgatacc acgggagaca cactagaaaa
10801 gtttgtggag ggtttgaagc tggatccggg gatggcctac ccatacgacg tgcctgacta
10861 cgcctcctag cggccgctcg agcatgcatc tagagggccc tattctatag tgtcacctaa
10921 atgctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
10981 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
11041 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
11101 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
11161 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
11221 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
11281 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
11341 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
11401 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
11461 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
11521 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
11581 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
11641 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
11701 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
11761 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
11821 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
11881 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
11941 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
12001 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat
12061 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
12121 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
12181 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
12241 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
12301 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
12361 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
12421 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
12481 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
12541 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
12601 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
12661 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
12721 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
12781 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
12841 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
12901 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
12961 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
13021 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
13081 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
13141 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
13201 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
13261 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
13321 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
13381 cgcgcgggga gaggcggttt gcgtattggg cgct
//
