| 质粒名称: | pΔΦ |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 60009 |
| 存储实验室: | David Russell |
|
载体骨架基本信息 |
|
| 载体名称: | pΔΦ is a deleted foamy vector pHSRV13 |
| 载体出品公司: | R. M. Flugel 1991 |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 3800 bp |
| 完整质粒大小: | -- |
| 载体修饰: | pΔΦ在gag、pol、env和bel1–3辅助基因以及LTR U3区域中有缺失，但保留了一个必需的2.5-kb顺式作用区域。另外，在gag序列中引入一个插入转基因盒的polylinker区域和终止密码子，以阻止病毒肽的表达，并消除gag-Pol融合蛋白的显性负效应。 |
| 载体类型: | 删除泡沫病毒 |
| 筛选标记: | 无；添加到磁带中 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pΔΦ was a gift from David Russell (Addgene plasmid # 60009 ; http://n2t.net/addgene:60009 ; RRID:Addgene_60009) |
| 参考文献部分: | Improved foamy virus vectors with minimal viral sequences. Trobridge G, Josephson N, Vassilopoulos G, Mac J, Russell DW. Mol Ther. 2002 Sep;6(3):321-8. S1525001602906726 [pii] PubMed 12231167 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS p-Delta-Phi 6290 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Deleted foamy virus vector plasmid containing a backbone with a
polylinker region for insertion of a transgene cassette. Ampicillin
resistant..
ACCESSION .
VERSION .
KEYWORDS p-Delta-Phi
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6290)
AUTHORS Trobridge G, Josephson N, Vassilopoulos G, Mac J, Russell DW
TITLE Improved foamy virus vectors with minimal viral sequences.
JOURNAL Mol Ther. 2002 Sep;6(3):321-8.
PUBMED 12231167
REFERENCE 2 (bases 1 to 6290)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6290
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 89..468
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 547..598
/label=CMVd2 promoter
/note="truncated human cytomegalovirus (CMV) immediate
early promoter that yields moderately reduced expression
relative to the full-length promoter"
primer_bind 549..569
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind complement(4296..4313)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4467..5055)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4547..4566)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5226..6086)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5849..5868
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6087..6191)
/gene="bla"
/label=AmpR promoter
primer_bind 6259..6277
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 ctcgaggagc ttggcccatt gcatacgttg tatccatatc ataatatgta catttatatt
61 ggctcatgtc caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa
121 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
181 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
241 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
301 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
361 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
421 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
481 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
541 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagcttc
601 tagattgtac gggagctctc ttcactactc gctgcgtcga gagtgtacga gactctccag
661 gtttggtaag aaatatttta tattgttata atgttactat gatccattaa cactctgctt
721 atagattgta agggtgattg caatgctttc tgcataaaac tttggttttc ttgttaatca
781 ataaaccgac ttgattcgag aacctactca tatattattg tctcttttat actttattaa
841 gtaaaaggat ttgtatatta gccttgctaa gggagacatc tagtgatata agtgtgaact
901 acacttatct taaatgatgt aactccttag gataatcaat atacaaaatt ccatgacaat
961 tggcgcccaa cgtggggctc gaatataagt cgggtttatt tgtaaattat ccctagggac
1021 ctccgagcat agcgggaggc atataaaagc caatagacaa tggctagcag gaagtaatgt
1081 tgaagaatat gaacttgatg ttgaagctct ggttgtaatt ttaagagata gaaatatacc
1141 aagaaatcct ttacatggag aagttatagg tcttcgcctt actgaaggat ggtggggaca
1201 aattgagaga tttcagatgg tacgttgatc taaggctatg gatttggcca tgggacaaga
1261 aatattagtt tatagtccca ttgtatctat gactaaaata caaaaaactc cactaccaga
1321 aagaaaagct ttacccatta gatggataac atggatgact tatttagaag atccaagaat
1381 ccaatttcat tatgataaaa ccttaccaga acttaagcat attccagatg tatatacatc
1441 tagtcagtct cctgttaaac atccttctca atatgaagga gtgttttata ctgatggctc
1501 ggccatcaaa agtcctgatc ctacaaaaag caataatgct ggcatgggaa tagtacatgc
1561 cacatacaaa cctgaatatc aagttttgaa tcaatggtca ataccactag gtaatcatac
1621 tgctcagatg gctgaaatag ctgcagttga atttgcctgt aaaaaagctt taaaaatacc
1681 tggtcctgta ttagttataa ctgatagttt ctatgtagca gaaagtgcta ataaagaatt
1741 accatactgg aaatctaatg ggtttgttaa taataagaaa aagcctctta aacatatctc
1801 caaatggaag tctattgctg agtgtttatc tatgaaacca gacattacta ttcaacatga
1861 aaaagggcat cagcctacaa ataccagtat tcatactgaa ggcaatgccc tagcagataa
1921 gcttgccacc caaggaagtt atgtagttaa ttgtaatacc aaaaaaccaa acctggatgc
1981 agagttggat caattattac agggtcatta tataaaagga tatcccaaac aatatacata
2041 ttttttagaa gatggcaaag taaaagtttc cagacctgaa ggggttaaaa ttattccccc
2101 tcagtcagac agacaaaaaa ttgtgcttca agcccacaat ttggctcaca ccggacgtga
2161 agccactctt ttaaaaattg ccaaccttta ttggtggcca aatatgagaa aggatgtggt
2221 taaacaacta ggacgctgtc aacagtgttt aatcacaaat gcttccaaca aagcctctgg
2281 tcctattcta agaccagata ggcctcaaaa accttttgat aaattcttta ttgactatat
2341 tggacctttg ccaccttcac agggatacct atatgtatta gtagttgttg atggaatgac
2401 aggattcact tggttatacc ccactaaggc tccttctact agcgcaactg ttaaatctct
2461 caatgtactc actagtattg caattccaaa ggtgattcac tctgatcaag gtgcagcatt
2521 cacttcttca acctttgctg aatgggcaaa ggaaagaggt atacatttgg aattcagtac
2581 tccttatcac ccccaaagta gtggtaaggt ggaaaggaaa aatagtgata taaaacgact
2641 tttaactaaa ctgctagtag gaagacccac aaagtggtat gacctattgc ctgttgtaca
2701 acttgcttta aacaacacct atagccctgt attaaaatat actccacatc aactcttatt
2761 tggtatagat tcaaatactc catttgcaaa tcaagataca cttgacttga ccagagaaga
2821 agaactttct cttttacagg aaattcgtac ttctttatac catccatcca cccctccaac
2881 ctcctctcgt tcctggtctc ctgttgttgg ccaattggtc caggagaggg tggctaggcc
2941 tgcttctttg agacctcgtt ggcataaacc gtctactgta cttaaggtgt tgaatccaag
3001 gactgttgtt attttggacc atcttggcaa caacagaact gtaagtatag ataatttaaa
3061 acctacttct catcagaatg gcaccaccaa tgacactgca acaatggatc atttggaaca
3121 aaatgaataa agcgcatgag gcacttcaga atacaacaac tgtgactgaa cagcagaagg
3181 aacaaattat actggacatt caaaatgaag aagtacaacc aactaggaga gataaattta
3241 gatatctgct ttatacttgt tgtgctacta gctcaagagt attggcctgg atatttttag
3301 tttgtatatt gttaatcatt gttttggttt catgctttgt gactatatcc agaatacaat
3361 ggaataagga tattcaggta ttaggacctg taatagactg gaatgttact caaagagctg
3421 tttatcaacc cttacagact agaaggattg cacgttccct tagaatgcag catcctgttc
3481 caaaatatgt ggaggtaaat atgactagta ttccacaagg tgtatactat gaaccccatc
3541 cggcctcgac ggtatcgatg gtaccggtcc gcggatccgc ggccgcgact ctagatcagg
3601 cgcgccgtta ccaagcagct atggaagctt atggacctca gagaggaagt aacgaggaga
3661 gggtgtggtg gaatgccact agaaaccagg gaaaacaagg aggagagtat tacagggaag
3721 gaggtgaaga acctcattac ccaaatactc ctgctcctca tagacgtacc tgggatgaga
3781 gacacaaggt tcttaaattg tcctcattcg ctactccctc tgacatccaa cgctgggcta
3841 ctaactctag attgtacggg agctctcttc actactcgct gcgtcgagag tgtacgagac
3901 tctccaggtt tggtaagaaa tattttatat tgttataatg ttactatgat ccattaacac
3961 tctgcttata gattgtaagg gtgattgcaa tgctttctgc ataaaacttt ggttttcttg
4021 ttaatcaata aaccgacttg attcgagaac ctactcatat attattgtct cttttatact
4081 ttattaagta aaaggatttg tatattagcc ttgctaaggg agacatctag tgatataagt
4141 gtgaactaca cttatcttaa atgatgtaac tccttaggat aatcaatata caaaattcca
4201 tgacaattgg cgataccgtc gaccgttcag ctgcattaat gaatcggcca acgcgcgggg
4261 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
4321 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
4381 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
4441 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
4501 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
4561 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
4621 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
4681 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
4741 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
4801 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
4861 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
4921 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
4981 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
5041 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
5101 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
5161 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
5221 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
5281 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
5341 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
5401 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
5461 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
5521 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
5581 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
5641 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
5701 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
5761 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
5821 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
5881 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
5941 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
6001 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
6061 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
6121 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
6181 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
6241 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca
//
