pY016 (pcDNA3.1-hLbCpf1)

LOCUS       pY016 (pcDNA3.1-hLbCpf1)	9227 bp 	DNA	SYN
DEFINITION  pY016 (pcDNA3.1-hLbCpf1)
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..9227
                     /organism="pY016 (pcDNA3.1-hLbCpf1)"
                     /mol_type="other DNA"
     promoter        236..852
                     /label="CMV_immearly_promoter"
     misc_feature    315..602
                     /label="CAG_enhancer"
     misc_feature    769..789
                     /label="CMV_fwd_primer"
     promoter        863..881
                     /label="T7_promoter"
     CDS             929..4750
                     /label="ORF frame 2"
     misc_feature    4667..4693
                     /label="HA"
     misc_feature    complement(4821..4838)
                     /label="BGH_rev_primer"
     terminator      4824..5051
                     /label="bGH_PA_terminator"
     rep_origin      5114..5420
                     /label="f1_origin"
     misc_feature    complement(5534..5554)
                     /label="pBABE_3_primer"
     misc_feature    complement(5540..5755)
                     /label="SV40_enhancer"
     promoter        5552..5820
                     /label="SV40_promoter"
     rep_origin      5719..5796
                     /label="SV40_origin"
     misc_feature    5781..5800
                     /label="SV40pro_F_primer"
     CDS             5935..6729
                     /label="ORF frame 1"
     gene            5938..6726
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
     CDS             complement(6244..6780)
                     /label="ORF frame 3"
     terminator      6906..7025
                     /label="SV40_PA_terminator"
     misc_feature    6994..7013
                     /label="EBV_rev_primer"
     promoter        complement(7069..7087)
                     /label="M13_reverse_primer"
     misc_feature    complement(7086..7108)
                     /label="M13_pUC_rev_primer"
     promoter        complement(7122..7151)
                     /label="lac_promoter"
     gene            complement(8231..9091)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(8231..9091)
                     /label="ORF frame 2"
     promoter        complement(9133..9161)
                     /label="AmpR_promoter"
ORIGIN
    1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
   61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
  121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
  181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
  241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
  301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
  361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
  421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
  481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
  541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
  601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
  661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
  721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
  781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
  841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
  901 gtttaaactt aagcttggta ccgccaccAT GAGCAAGCTG GAGAAGTTTA CAAACTGCTA
  961 CTCCCTGTCT AAGACCCTGA GGTTCAAGGC CATCCCTGTG GGCAAGACCC AGGAGAACAT
 1021 CGACAATAAG CGGCTGCTGG TGGAGGACGA GAAGAGAGCC GAGGATTATA AGGGCGTGAA
 1081 GAAGCTGCTG GATCGCTACT ATCTGTCTTT TATCAACGAC GTGCTGCACA GCATCAAGCT
 1141 GAAGAATCTG AACAATTACA TCAGCCTGTT CCGGAAGAAA ACCAGAACCG AGAAGGAGAA
 1201 TAAGGAGCTG GAGAACCTGG AGATCAATCT GCGGAAGGAG ATCGCCAAGG CCTTCAAGGG
 1261 CAACGAGGGC TACAAGTCCC TGTTTAAGAA GGATATCATC GAGACAATCC TGCCAGAGTT
 1321 CCTGGACGAT AAGGACGAGA TCGCCCTGGT GAACAGCTTC AATGGCTTTA CCACAGCCTT
 1381 CACCGGCTTC TTTGATAACA GAGAGAATAT GTTTTCCGAG GAGGCCAAGA GCACATCCAT
 1441 CGCCTTCAGG TGTATCAACG AGAATCTGAC CCGCTACATC TCTAATATGG ACATCTTCGA
 1501 GAAGGTGGAC GCCATCTTTG ATAAGCACGA GGTGCAGGAG ATCAAGGAGA AGATCCTGAA
 1561 CAGCGACTAT GATGTGGAGG ATTTCTTTGA GGGCGAGTTC TTTAACTTTG TGCTGACACA
 1621 GGAGGGCATC GACGTGTATA ACGCCATCAT CGGCGGCTTC GTGACCGAGA GCGGCGAGAA
 1681 GATCAAGGGC CTGAACGAGT ACATCAACCT GTATAATCAG AAAACCAAGC AGAAGCTGCC
 1741 TAAGTTTAAG CCACTGTATA AGCAGGTGCT GAGCGATCGG GAGTCTCTGA GCTTCTACGG
 1801 CGAGGGCTAT ACATCCGATG AGGAGGTGCT GGAGGTGTTT AGAAACACCC TGAACAAGAA
 1861 CAGCGAGATC TTCAGCTCCA TCAAGAAGCT GGAGAAGCTG TTCAAGAATT TTGACGAGTA
 1921 CTCTAGCGCC GGCATCTTTG TGAAGAACGG CCCCGCCATC AGCACAATCT CCAAGGATAT
 1981 CTTCGGCGAG TGGAACGTGA TCCGGGACAA GTGGAATGCC GAGTATGACG ATATCCACCT
 2041 GAAGAAGAAG GCCGTGGTGA CCGAGAAGTA CGAGGACGAT CGGAGAAAGT CCTTCAAGAA
 2101 GATCGGCTCC TTTTCTCTGG AGCAGCTGCA GGAGTACGCC GACGCCGATC TGTCTGTGGT
 2161 GGAGAAGCTG AAGGAGATCA TCATCCAGAA GGTGGATGAG ATCTACAAGG TGTATGGCTC
 2221 CTCTGAGAAG CTGTTCGACG CCGATTTTGT GCTGGAGAAG AGCCTGAAGA AGAACGACGC
 2281 CGTGGTGGCC ATCATGAAGG ACCTGCTGGA TTCTGTGAAG AGCTTCGAGA ATTACATCAA
 2341 GGCCTTCTTT GGCGAGGGCA AGGAGACAAA CAGGGACGAG TCCTTCTATG GCGATTTTGT
 2401 GCTGGCCTAC GACATCCTGC TGAAGGTGGA CCACATCTAC GATGCCATCC GCAATTATGT
 2461 GACCCAGAAG CCCTACTCTA AGGATAAGTT CAAGCTGTAT TTTCAGAACC CTCAGTTCAT
 2521 GGGCGGCTGG GACAAGGATA AGGAGACAGA CTATCGGGCC ACCATCCTGA GATACGGCTC
 2581 CAAGTACTAT CTGGCCATCA TGGATAAGAA GTACGCCAAG TGCCTGCAGA AGATCGACAA
 2641 GGACGATGTG AACGGCAATT ACGAGAAGAT CAACTATAAG CTGCTGCCCG GCCCTAATAA
 2701 GATGCTGCCA AAGGTGTTCT TTTCTAAGAA GTGGATGGCC TACTATAACC CCAGCGAGGA
 2761 CATCCAGAAG ATCTACAAGA ATGGCACATT CAAGAAGGGC GATATGTTTA ACCTGAATGA
 2821 CTGTCACAAG CTGATCGACT TCTTTAAGGA TAGCATCTCC CGGTATCCAA AGTGGTCCAA
 2881 TGCCTACGAT TTCAACTTTT CTGAGACAGA GAAGTATAAG GACATCGCCG GCTTTTACAG
 2941 AGAGGTGGAG GAGCAGGGCT ATAAGGTGAG CTTCGAGTCT GCCAGCAAGA AGGAGGTGGA
 3001 TAAGCTGGTG GAGGAGGGCA AGCTGTATAT GTTCCAGATC TATAACAAGG ACTTTTCCGA
 3061 TAAGTCTCAC GGCACACCCA ATCTGCACAC CATGTACTTC AAGCTGCTGT TTGACGAGAA
 3121 CAATCACGGA CAGATCAGGC TGAGCGGAGG AGCAGAGCTG TTCATGAGGC GCGCCTCCCT
 3181 GAAGAAGGAG GAGCTGGTGG TGCACCCAGC CAACTCCCCT ATCGCCAACA AGAATCCAGA
 3241 TAATCCCAAG AAAACCACAA CCCTGTCCTA CGACGTGTAT AAGGATAAGA GGTTTTCTGA
 3301 GGACCAGTAC GAGCTGCACA TCCCAATCGC CATCAATAAG TGCCCCAAGA ACATCTTCAA
 3361 GATCAATACA GAGGTGCGCG TGCTGCTGAA GCACGACGAT AACCCCTATG TGATCGGCAT
 3421 CGATAGGGGC GAGCGCAATC TGCTGTATAT CGTGGTGGTG GACGGCAAGG GCAACATCGT
 3481 GGAGCAGTAT TCCCTGAACG AGATCATCAA CAACTTCAAC GGCATCAGGA TCAAGACAGA
 3541 TTACCACTCT CTGCTGGACA AGAAGGAGAA GGAGAGGTTC GAGGCCCGCC AGAACTGGAC
 3601 CTCCATCGAG AATATCAAGG AGCTGAAGGC CGGCTATATC TCTCAGGTGG TGCACAAGAT
 3661 CTGCGAGCTG GTGGAGAAGT ACGATGCCGT GATCGCCCTG GAGGACCTGA ACTCTGGCTT
 3721 TAAGAATAGC CGCGTGAAGG TGGAGAAGCA GGTGTATCAG AAGTTCGAGA AGATGCTGAT
 3781 CGATAAGCTG AACTACATGG TGGACAAGAA GTCTAATCCT TGTGCAACAG GCGGCGCCCT
 3841 GAAGGGCTAT CAGATCACCA ATAAGTTCGA GAGCTTTAAG TCCATGTCTA CCCAGAACGG
 3901 CTTCATCTTT TACATCCCTG CCTGGCTGAC ATCCAAGATC GATCCATCTA CCGGCTTTGT
 3961 GAACCTGCTG AAAACCAAGT ATACCAGCAT CGCCGATTCC AAGAAGTTCA TCAGCTCCTT
 4021 TGACAGGATC ATGTACGTGC CCGAGGAGGA TCTGTTCGAG TTTGCCCTGG ACTATAAGAA
 4081 CTTCTCTCGC ACAGACGCCG ATTACATCAA GAAGTGGAAG CTGTACTCCT ACGGCAACCG
 4141 GATCAGAATC TTCCGGAATC CTAAGAAGAA CAACGTGTTC GACTGGGAGG AGGTGTGCCT
 4201 GACCAGCGCC TATAAGGAGC TGTTCAACAA GTACGGCATC AATTATCAGC AGGGCGATAT
 4261 CAGAGCCCTG CTGTGCGAGC AGTCCGACAA GGCCTTCTAC TCTAGCTTTA TGGCCCTGAT
 4321 GAGCCTGATG CTGCAGATGC GGAACAGCAT CACAGGCCGC ACCGACGTGG ATTTTCTGAT
 4381 CAGCCCTGTG AAGAACTCCG ACGGCATCTT CTACGATAGC CGGAACTATG AGGCCCAGGA
 4441 GAATGCCATC CTGCCAAAGA ACGCCGACGC CAATGGCGCC TATAACATCG CCAGAAAGGT
 4501 GCTGTGGGCC ATCGGCCAGT TCAAGAAGGC CGAGGACGAG AAGCTGGATA AGGTGAAGAT
 4561 CGCCATCTCT AACAAGGAGT GGCTGGAGTA CGCCCAGACC AGCGTGAAGC ACAAAAGGCC
 4621 GGCGGCCACG AAAAAGGCCG GCCAGGCAAA AAAGAAAAAG GGATCCTACC CATACGATGT
 4681 TCCAGATTAC GCTTATCCCT ACGACGTGCC TGATTATGCA TACCCATATG ATGTCCCCGA
 4741 CTATGCCTAA Gaattctgca gatatccagc acagtggcgg ccgctcgagt ctagagggcc
 4801 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
 4861 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
 4921 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
 4981 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
 5041 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
 5101 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
 5161 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
 5221 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
 5281 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
 5341 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
 5401 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
 5461 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
 5521 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
 5581 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
 5641 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
 5701 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
 5761 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
 5821 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
 5881 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
 5941 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
 6001 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
 6061 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
 6121 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
 6181 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
 6241 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
 6301 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
 6361 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
 6421 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
 6481 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
 6541 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
 6601 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
 6661 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
 6721 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
 6781 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
 6841 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
 6901 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
 6961 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
 7021 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
 7081 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
 7141 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
 7201 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
 7261 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
 7321 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
 7381 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
 7441 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
 7501 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
 7561 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
 7621 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
 7681 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
 7741 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
 7801 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
 7861 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
 7921 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
 7981 gatccggcaa acaaaccacc gctggtagcg gtttttttgt ttgcaagcag cagattacgc
 8041 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
 8101 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
 8161 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
 8221 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
 8281 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
 8341 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
 8401 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
 8461 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
 8521 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
 8581 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
 8641 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
 8701 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
 8761 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
 8821 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
 8881 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
 8941 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
 9001 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
 9061 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
 9121 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
 9181 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//