质粒名称: | pLSC-5 |
---|---|
出品公司: | 爱迪基因 |
目录编号: | 62889 |
存储实验室: | Feng Zhang |
载体骨架基本信息 |
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载体名称: | lentiCRISPR |
载体出品公司: | Zhang lab |
载体抗性: | 氨苄霉素 |
空载体大小: | 8809 bp |
完整质粒大小: | 13792 bp |
载体类型: | 哺乳细胞表达 |
筛选标记: | 嘌呤霉素 |
高拷贝/低拷贝: | 未知 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | SpCas9(574-1368) |
物种来源: | 光合细菌 Rhodopseudomonas palustris |
插入大小: | 2916 bp |
基因突变: | *see comment below |
GenBank编号 | JN247409 |
启动子 | EFS |
标签/融合蛋白: | NLS SV40(N端) FFBP12(N端) P2A(C端) |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | Age1(未破坏) |
3' 克隆位点: | BamH1(未破坏) |
5' 测序引物 | NA |
3' 测序引物 | |
文献引用方法 |
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材料和方法部分: | pLSC-5 was a gift from Feng Zhang (Addgene plasmid # 62889) |
参考文献部分: | A split-Cas9 architecture for inducible genome editing and transcription modulation. Zetsche B, Volz SE, Zhang F. Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149. 10.1038/nbt.3149 PubMed 25643054 |
pLSC-5
价格:1200元
联系方式:I47-825O-882O
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质粒基本信息
质粒图谱信息
质粒简介
质粒序列
LOCUS pLSC-5 13792 bp DNA SYN DEFINITION pLSC-5 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..13792 /organism="pLSC-5" /mol_type="other DNA" promoter 239..815 /label="CMV_immearly_promoter" misc_feature 318..605 /label="CAG_enhancer" misc_feature 772..792 /label="CMV_fwd_primer" misc_feature 835..1015 /label="HIV-1_5_LTR" misc_feature 835..1015 /label="truncHIV-1_3_LTR" misc_feature 1126..1170 /label="HIV-1_psi_pack" CDS 1558..2445 /label="ORF frame 1" misc_feature 1680..1913 /label="RRE" misc_feature 2444..2459 /label="cPPT" promoter 2617..2849 /label="hU6_promoter" misc_feature 2779..2798 /label="LKO1_5_primer" CDS complement(2962..4452) /label="ORF frame 2" CDS 3235..8868 /label="ORF frame 1" misc_feature 3241..3261 /label="SV40 NLS" misc_feature 3241..3264 /label="SV40 NLS" misc_feature 3247..3261 /label="NLS" gene 8281..8868 /label="puro (variant)" /gene="puro (variant)" misc_feature 8886..9461 /label="WPRE" CDS 8974..9483 /label="ORF frame 1" CDS 8987..9517 /label="ORF frame 2" misc_feature 9527..9542 /label="cPPT" misc_feature 9527..9548 /label="U3PPT" misc_feature 9544..9596 /label="delta_U3" misc_feature 9597..9777 /label="truncHIV-1_3_LTR" misc_feature 9597..9777 /label="HIV-1_5_LTR" misc_feature complement(9803..9820) /label="BGH_rev_primer" terminator 9806..10033 /label="bGH_PA_terminator" rep_origin 10096..10402 /label="f1_origin" misc_feature complement(10516..10536) /label="pBABE_3_primer" misc_feature complement(10522..10737) /label="SV40_enhancer" promoter 10534..10802 /label="SV40_promoter" rep_origin 10701..10778 /label="SV40_origin" misc_feature 10763..10782 /label="SV40pro_F_primer" promoter 10896..10963 /label="EM7_promoter" misc_feature 10964..11338 /label="sh_ble" gene 10964..11335 /label="bleo" /gene="bleo" terminator 11471..11590 /label="SV40_PA_terminator" misc_feature 11559..11578 /label="EBV_rev_primer" promoter complement(11634..11652) /label="M13_reverse_primer" misc_feature complement(11651..11673) /label="M13_pUC_rev_primer" promoter complement(11687..11716) /label="lac_promoter" gene complement(12799..13659) /label="Ampicillin" /gene="Ampicillin" CDS complement(12799..13659) /label="ORF frame 2" promoter complement(13701..13729) /label="AmpR_promoter" ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta aggtaccgag ggcctatttc ccatgattcc ttcatatttg 2641 catatacgat acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag 2701 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta 2761 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc 2821 ttggctttat atatcttGTG GAAAGGACGA AACACCgGAG ACGgaCGTCT Ctgttttaga 2881 gctaGAAAta gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga 2941 gtcggtgcTT TTTTgaattc gctagctagg tcttgaaagg agtgggaatt ggctccggtg 3001 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 3061 gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 3121 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 3181 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggaccggtgc caccATGGCC 3241 CCAAAGAAGA AGCGGAAGGT CGGTATCCAC GGAGTCCCAG CAGCCGGCGT GCAGGTGGAG 3301 ACCATCAGCC CCGGCGACGG CAGAACCTTC CCCAAGAGAG GCCAGACCTG CGTGGTGCAC 3361 TACACCGGCA TGCTGGAGGA CGGCAAGAAG TTCGACAGCA GCAGAGACAG AAACAAGCCC 3421 TTCAAGTTCA TGCTGGGCAA GCAGGAGGTG ATCAGAGGCT GGGAGGAGGG CGTGGCCCAG 3481 ATGAGCGTGG GCCAGAGAGC CAAGCTGACC ATCAGCCCCG ACTACGCCTA CGGCGCCACC 3541 GGCCACCCCG GCATCATCCC TCCTCACGCC ACCCTGGTGT TCGACGTGGA GCTGCTGAAG 3601 CTGGAGGGTG GCGGTGGCtc gGGTGGCGGT GGCtcgGGTG GCGGTGGCtc gTGCTTCGAC 3661 TCCGTGGAAA TCTCCGGCGT GGAAGATCGG TTCAACGCCT CCCTGGGCGC ATACCACGAT 3721 CTGCTGAAAA TTATCAAGGA CAAGGACTTC CTGGACAATG AGGAAAACGA GGACATTCTG 3781 GAAGATATCG TGCTGACCCT GACACTGTTT GAGGACAGAG AGATGATCGA GGAACGGCTG 3841 AAAACCTATG CCCACCTGTT CGACGACAAA GTGATGAAGC AGCTGAAGCG GCGGAGATAC 3901 ACCGGCTGGG GCAGGCTGAG CCGGAAGCTG ATCAACGGCA TCCGGGACAA GCAGTCCGGC 3961 AAGACAATCC TGGATTTCCT GAAGTCCGAC GGCTTCGCCA ACAGAAACTT CATGCAGCTG 4021 ATCCACGACG ACAGCCTGAC CTTTAAAGAG GACATCCAGA AAGCCCAGGT GTCCGGCCAG 4081 GGCGATAGCC TGCACGAGCA CATTGCCAAT CTGGCCGGCA GCCCCGCCAT TAAGAAGGGC 4141 ATCCTGCAGA CAGTGAAGGT GGTGGACGAG CTCGTGAAAG TGATGGGCCG GCACAAGCCC 4201 GAGAACATCG TGATCGAAAT GGCCAGAGAG AACCAGACCA CCCAGAAGGG ACAGAAGAAC 4261 AGCCGCGAGA GAATGAAGCG GATCGAAGAG GGCATCAAAG AGCTGGGCAG CCAGATCCTG 4321 AAAGAACACC CCGTGGAAAA CACCCAGCTG CAGAACGAGA AGCTGTACCT GTACTACCTG 4381 CAGAATGGGC GGGATATGTA CGTGGACCAG GAACTGGACA TCAACCGGCT GTCCGACTAC 4441 GATGTGGACC ATATCGTGCC TCAGAGCTTT CTGAAGGACG ACTCCATCGA CAACAAGGTG 4501 CTGACCAGAA GCGACAAGAA CCGGGGCAAG AGCGACAACG TGCCCTCCGA AGAGGTCGTG 4561 AAGAAGATGA AGAACTACTG GCGGCAGCTG CTGAACGCCA AGCTGATTAC CCAGAGAAAG 4621 TTCGACAATC TGACCAAGGC CGAGAGAGGC GGCCTGAGCG AACTGGATAA GGCCGGCTTC 4681 ATCAAGAGAC AGCTGGTGGA AACCCGGCAG ATCACAAAGC ACGTGGCACA GATCCTGGAC 4741 TCCCGGATGA ACACTAAGTA CGACGAGAAT GACAAGCTGA TCCGGGAAGT GAAAGTGATC 4801 ACCCTGAAGT CCAAGCTGGT GTCCGATTTC CGGAAGGATT TCCAGTTTTA CAAAGTGCGC 4861 GAGATCAACA ACTACCACCA CGCCCACGAC GCCTACCTGA ACGCCGTCGT GGGAACCGCC 4921 CTGATCAAAA AGTACCCTAA GCTGGAAAGC GAGTTCGTGT ACGGCGACTA CAAGGTGTAC 4981 GACGTGCGGA AGATGATCGC CAAGAGCGAG CAGGAAATCG GCAAGGCTAC CGCCAAGTAC 5041 TTCTTCTACA GCAACATCAT GAACTTTTTC AAGACCGAGA TTACCCTGGC CAACGGCGAG 5101 ATCCGGAAGC GGCCTCTGAT CGAGACAAAC GGCGAAACCG GGGAGATCGT GTGGGATAAG 5161 GGCCGGGATT TTGCCACCGT GCGGAAAGTG CTGAGCATGC CCCAAGTGAA TATCGTGAAA 5221 AAGACCGAGG TGCAGACAGG CGGCTTCAGC AAAGAGTCTA TCCTGCCCAA GAGGAACAGC 5281 GATAAGCTGA TCGCCAGAAA GAAGGACTGG GACCCTAAGA AGTACGGCGG CTTCGACAGC 5341 CCCACCGTGG CCTATTCTGT GCTGGTGGTG GCCAAAGTGG AAAAGGGCAA GTCCAAGAAA 5401 CTGAAGAGTG TGAAAGAGCT GCTGGGGATC ACCATCATGG AAAGAAGCAG CTTCGAGAAG 5461 AATCCCATCG ACTTTCTGGA AGCCAAGGGC TACAAAGAAG TGAAAAAGGA CCTGATCATC 5521 AAGCTGCCTA AGTACTCCCT GTTCGAGCTG GAAAACGGCC GGAAGAGAAT GCTGGCCTCT 5581 GCCGGCGAAC TGCAGAAGGG AAACGAACTG GCCCTGCCCT CCAAATATGT GAACTTCCTG 5641 TACCTGGCCA GCCACTATGA GAAGCTGAAG GGCTCCCCCG AGGATAATGA GCAGAAACAG 5701 CTGTTTGTGG AACAGCACAA GCACTACCTG GACGAGATCA TCGAGCAGAT CAGCGAGTTC 5761 TCCAAGAGAG TGATCCTGGC CGACGCTAAT CTGGACAAAG TGCTGTCCGC CTACAACAAG 5821 CACCGGGATA AGCCCATCAG AGAGCAGGCC GAGAATATCA TCCACCTGTT TACCCTGACC 5881 AATCTGGGAG CCCCTGCCGC CTTCAAGTAC TTTGACACCA CCATCGACCG GAAGAGGTAC 5941 ACCAGCACCA AAGAGGTGCT GGATGCCACC CTGATCCACC AGAGCATCAC CGGCCTGTAC 6001 GAGACACGGA TCGACCTGTC TCAGCTGGGA GGCGACAAAA GGCCGGCGGC CACGAAAAAG 6061 GCCGGCCAGG CAAAAAAGAA AAAGGGATCC GGCGCAACAA ACTTCTCTCT GCTGAAACAA 6121 GCCGGAGATG TCGAAGAGAA TCCTGGACCG CTAGATTTAG CTAGCTTAAT CCTCGACAAG 6181 AAGTACAGCA TCGGCCTGGA CATCGGCACC AACTCTGTGG GCTGGGCCGT GATCACCGAC 6241 GAGTACAAGG TGCCCAGCAA GAAATTCAAG GTGCTGGGCA ACACCGACCG GCACAGCATC 6301 AAGAAGAACC TGATCGGAGC CCTGCTGTTC GACAGCGGCG AAACAGCCGA GGCCACCCGG 6361 CTGAAGAGAA CCGCCAGAAG AAGATACACC AGACGGAAGA ACCGGATCTG CTATCTGCAA 6421 GAGATCTTCA GCAACGAGAT GGCCAAGGTG GACGACAGCT TCTTCCACAG ACTGGAAGAG 6481 TCCTTCCTGG TGGAAGAGGA TAAGAAGCAC GAGCGGCACC CCATCTTCGG CAACATCGTG 6541 GACGAGGTGG CCTACCACGA GAAGTACCCC ACCATCTACC ACCTGAGAAA GAAACTGGTG 6601 GACAGCACCG ACAAGGCCGA CCTGCGGCTG ATCTATCTGG CCCTGGCCCA CATGATCAAG 6661 TTCCGGGGCC ACTTCCTGAT CGAGGGCGAC CTGAACCCCG ACAACAGCGA CGTGGACAAG 6721 CTGTTCATCC AGCTGGTGCA GACCTACAAC CAGCTGTTCG AGGAAAACCC CATCAACGCC 6781 AGCGGCGTGG ACGCCAAGGC CATCCTGTCT GCCAGACTGA GCAAGAGCAG ACGGCTGGAA 6841 AATCTGATCG CCCAGCTGCC CGGCGAGAAG AAGAATGGCC TGTTCGGAAA CCTGATTGCC 6901 CTGAGCCTGG GCCTGACCCC CAACTTCAAG AGCAACTTCG ACCTGGCCGA GGATGCCAAA 6961 CTGCAGCTGA GCAAGGACAC CTACGACGAC GACCTGGACA ACCTGCTGGC CCAGATCGGC 7021 GACCAGTACG CCGACCTGTT TCTGGCCGCC AAGAACCTGT CCGACGCCAT CCTGCTGAGC 7081 GACATCCTGA GAGTGAACAC CGAGATCACC AAGGCCCCCC TGAGCGCCTC TATGATCAAG 7141 AGATACGACG AGCACCACCA GGACCTGACC CTGCTGAAAG CTCTCGTGCG GCAGCAGCTG 7201 CCTGAGAAGT ACAAAGAGAT TTTCTTCGAC CAGAGCAAGA ACGGCTACGC CGGCTACATT 7261 GACGGCGGAG CCAGCCAGGA AGAGTTCTAC AAGTTCATCA AGCCCATCCT GGAAAAGATG 7321 GACGGCACCG AGGAACTGCT CGTGAAGCTG AACAGAGAGG ACCTGCTACG GAAGCAGCGG 7381 ACCTTCGACA ACGGCAGCAT CCCCCACCAG ATCCACCTGG GAGAGCTGCA CGCCATTCTG 7441 CGGCGGCAGG AAGATTTTTA CCCATTCCTG AAGGACAACC GGGAAAAGAT CGAGAAGATC 7501 CTGACCTTCC GCATCCCCTA CTACGTGGGC CCTCTGGCCA GGGGAAACAG CAGATTCGCC 7561 TGGATGACCA GAAAGAGCGA GGAAACCATC ACCCCCTGGA ACTTCGAGGA AGTGGTGGAC 7621 AAGGGCGCTT CCGCCCAGAG CTTCATCGAG CGGATGACCA ACTTCGATAA GAACCTGCCC 7681 AACGAGAAGG TGCTGCCCAA GCACAGCCTG CTGTACGAGT ACTTCACCGT GTATAACGAG 7741 CTGACCAAAG TGAAATACGT GACCGAGGGA ATGAGAAAGC CCGCCTTCCT GAGCGGCGAG 7801 CAGAAAAAGG CCATCGTGGA CCTGCTGTTC AAGACCAACC GGAAAGTGAC CGTGAAGCAG 7861 CTGAAAGAGG ACTACTTCAA GAAAATCGAG GGTGGCGGTG GCtcgGGTGG CGGTGGCtcg 7921 GGTGGCGGTG GCtcgGAGAT GTGGCACGAG GGCCTGGAGG AGGCCAGCAG ACTGTACTTC 7981 GGCGAGAGAA ACGTGAAGGG CATGTTCGAG GTGCTGGAGC CCCTGCACGC CATGATGGAG 8041 AGAGGCCCCC AGACCCTGAA GGAGACCAGC TTCAACCAGG CCTACGGCAG AGACCTGATG 8101 GAGGCCCAGG AGTGGTGCAG AAAGTACATG AAGAGCGGCA ACGTGAAGGA CCTGACCCAG 8161 GCCTGGGACC TGTACTACCA CGTGTTCAGA AGAATCAGCA AGCAGGGATC CGGCGCAACA 8221 AACTTCTCTC TGCTGAAACA AGCCGGAGAT GTCGAAGAGA ATCCTGGACC GACCGAGTAC 8281 AAGCCCACGG TGCGCCTCGC CACCCGCGAC GACGTCCCCA GGGCCGTACG CACCCTCGCC 8341 GCCGCGTTCG CCGACTACCC CGCCACGCGC CACACCGTCG ATCCGGACCG CCACATCGAG 8401 CGGGTCACCG AGCTGCAAGA ACTCTTCCTC ACGCGCGTCG GGCTCGACAT CGGCAAGGTG 8461 TGGGTCGCGG ACGACGGCGC CGCGGTGGCG GTCTGGACCA CGCCGGAGAG CGTCGAAGCG 8521 GGGGCGGTGT TCGCCGAGAT CGGCCCGCGC ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC 8581 GCGCAGCAAC AGATGGAAGG CCTCCTGGCG CCGCACCGGC CCAAGGAGCC CGCGTGGTTC 8641 CTGGCCACCG TCGGAGTCTC GCCCGACCAC CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG 8701 CTCCCCGGAG TGGAGGCGGC CGAGCGCGCC GGGGTGCCCG CCTTCCTGGA GACCTCCGCG 8761 CCCCGCAACC TCCCCTTCTA CGAGCGGCTC GGCTTCACCG TCACCGCCGA CGTCGAGGTG 8821 CCCGAAGGAC CGCGCACCTG GTGCATGACC CGCAAGCCCG GTGCCTGAAC GCGTTAAGTC 8881 GACAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT 8941 GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC 9001 CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG 9061 TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC 9121 ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC 9181 CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG 9241 CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAAT CATCGTCCTT TCCTTGGCTG 9301 CTCGCCTGTG TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC 9361 CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT 9421 CTTCGCCTTC GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCGTCGACTT 9481 TAAGACCAAT GACTTACAAG GCAGCTGTAG ATCTTAGCCA CTTTTTAAAA GAAAAGGGGG 9541 GACTGGAAGG GCTAATTCAC TCCCAACGAA GACAAGATCT GCTTTTTGCT TGTACTGGGT 9601 CTCTCTGGTT AGACCAGATC TGAGCCTGGG AGCTCTCTGG CTAACTAGGG AACCCACTGC 9661 TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG 9721 ACTCTGGTAA CTAGAGATCC CTCAGACCCT TTTAGTCAGT GTGGAAAATC TCTAGCAGgg 9781 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt 9841 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa 9901 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg 9961 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg 10021 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac 10081 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 10141 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 10201 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 10261 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 10321 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 10381 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 10441 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 10501 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag 10561 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc 10621 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 10681 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 10741 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc 10801 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg 10861 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata 10921 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag 10981 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg 11041 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt 11101 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt 11161 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt 11221 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt 11281 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca 11341 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt 11401 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc 11461 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 11521 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 11581 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc 11641 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 11701 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 11761 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 11821 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 11881 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 11941 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 12001 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 12061 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 12121 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 12181 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 12241 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 12301 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 12361 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 12421 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 12481 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 12541 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 12601 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 12661 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 12721 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 12781 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 12841 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 12901 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 12961 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 13021 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 13081 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 13141 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 13201 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 13261 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 13321 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 13381 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 13441 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 13501 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 13561 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 13621 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 13681 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 13741 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ac //