质粒名称: | pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE |
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出品公司: | 爱迪基因 |
目录编号: | 60910 |
存储实验室: | Ron Vale |
载体骨架基本信息 |
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载体名称: | pHR |
载体抗性: | 氨苄霉素 |
载体类型: | 哺乳细胞表达 |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | dCas9 |
启动子 | dSV40 |
标签/融合蛋白: | NLS(N端) HA(C端) 2x NLS(C端) |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | 未知 |
3' 克隆位点: | BamHI (未破坏) |
5' 测序引物 | SV40pro-F2 |
3' 测序引物 | mTagBFP-R |
文献引用方法 |
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材料和方法部分: | pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE was a gift from Ron Vale (Addgene plasmid # 60910) |
参考文献部分: | A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging. Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD. Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039. 10.1016/j.cell.2014.09.039 PubMed 25307933 |
pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE
价格:1200元
联系方式:I47-825O-882O
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LOCUS pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE 14911 bp DNA SYN DEFINITION pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..14911 /organism="pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE" /mol_type="other DNA" misc_feature complement(103..120) /label="Sp6_primer" gene 358..621 /label="tet (300 - 563)" /gene="tet (300 - 563)" misc_feature complement(410..429) /label="pBRrevBam_primer" misc_feature 763..785 /label="pGEX_3_primer" promoter 944..972 /label="AmpR_promoter" gene 1014..1874 /label="Ampicillin" /gene="Ampicillin" CDS 1014..1874 /label="ORF frame 3" rep_origin 2029..2648 /label="pBR322_origin" misc_feature complement(2874..2894) /label="pBABE_3_primer" misc_feature complement(2880..3333) /label="SV40_enhancer" promoter 2892..3160 /label="SV40_promoter" rep_origin 3059..3136 /label="SV40_origin" misc_feature 3121..3140 /label="SV40pro_F_primer" misc_feature 4341..4356 /label="SV40_int" misc_feature 4362..4409 /label="SV40_3_splice" CDS 4486..4959 /label="ORF frame 1" misc_feature 4537..4557 /label="SV40 NLS" misc_feature 4543..4557 /label="NLS" terminator 4985..5116 /label="SV40_PA_terminator" misc_feature 5073..5092 /label="EBV_rev_primer" misc_feature 5738..5918 /label="HIV-1_5_LTR" misc_feature 5738..5918 /label="truncHIV-1_3_LTR" misc_feature 6029..6073 /label="HIV-1_psi_pack" CDS 6460..7557 /label="ORF frame 1" misc_feature 6582..6815 /label="RRE" misc_feature 7333..7348 /label="cPPT" promoter 7531..7710 /label="SV40_promoter" rep_origin 7578..7655 /label="SV40_origin" misc_feature 7640..7659 /label="SV40pro_F_primer" CDS 7732..14640 /label="ORF frame 1" misc_feature 7735..7758 /label="SV40 NLS" misc_feature 7735..7755 /label="SV40 NLS" misc_feature 7741..7755 /label="NLS" CDS complement(7774..12114) /label="ORF frame 2" misc_feature 11917..11943 /label="HA" CDS complement(13720..14445) /label="ORF frame 2" misc_feature 14738..14759 /label="U3PPT" misc_feature 14738..14753 /label="cPPT" misc_feature 14755..14807 /label="delta_U3" misc_feature 14808..77 /label="HIV-1_5_LTR" misc_feature 14808..77 /label="truncHIV-1_3_LTR" ORIGIN 1 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt 61 gtggaaaatc tctagcagca tctagaatta attccgtgta ttctatagtg tcacctaaat 121 cgtatgtgta tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg 181 tacaagccta attgtgtagc atctggctta ctgaagcaga ccctatcatc tctctcgtaa 241 actgccgtca gagtcggttt ggttggacga accttctgag tttctggtaa cgccgtcccg 301 cacccggaaa tggtcagcga accaatcagc agggtcatcg ctagccagat cctctacgcc 361 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc 421 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc 481 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca 541 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg 601 caggagtcgc ataagggaga gcgtcgaatg gtgcactctc agtacaatct agctctgatg 661 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt 721 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc 781 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat 841 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg 901 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc 961 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta 1021 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg 1081 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg 1141 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac 1201 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg 1261 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt 1321 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg 1381 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac 1441 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt 1501 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag 1561 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc 1621 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 1681 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 1741 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 1801 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 1861 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac 1921 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa 1981 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat 2041 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 2101 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 2161 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc 2221 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg 2281 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg 2341 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 2401 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg 2461 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga 2521 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 2581 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 2641 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc 2701 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg 2761 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc 2821 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt 2881 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 2941 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag 3001 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat 3061 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt 3121 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg 3181 cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat 3241 gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact 3301 catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc 3361 cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat 3421 acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc 3481 cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt 3541 actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca 3601 cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat 3661 cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc 3721 aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta 3781 tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt 3841 cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct 3901 ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga 3961 cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga 4021 cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta 4081 aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg 4141 acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg 4201 acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg 4261 ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct 4321 acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta 4381 ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag 4441 tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat 4501 gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa 4561 gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat 4621 agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag 4681 aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac 4741 atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa 4801 aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat 4861 agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt 4921 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt 4981 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 5041 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 5101 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac 5161 cccataccct attaccactg ccaattacct agtggtttca tttactctaa acctgtgatt 5221 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag 5281 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac 5341 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat 5401 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag 5461 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac 5521 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc 5581 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt 5641 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc 5701 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat 5761 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt 5821 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc 5881 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg 5941 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc 6001 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc 6061 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga 6121 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta 6181 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa 6241 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt 6301 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa 6361 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca 6421 cagcaagcgg ccggtgatct tcagacctgg acgatatata tgagggacaa ttggagaagt 6481 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca 6541 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg 6601 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc 6661 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg 6721 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg 6781 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa 6841 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag 6901 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta 6961 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg 7021 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat 7081 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta 7141 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc 7201 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac 7261 agagacagat ccattcgatt agtgaacgga tctcgacggt ATcgccaaat ggcagtattc 7321 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta 7381 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa 7441 aattttcggg tttattacag ggacagcaga gatccagttt ggatcgataa gcttgatatc 7501 gaattcaggc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc 7561 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 7621 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat 7681 tccagaagta gtgaggaggc ttttttggag gcctaggcta cgcgcGCCAC CATGCCCAAG 7741 AAGAAGCGCA AGGTGGGacg cgtctgcagg atatcaagct tgcggtaccg cgggcccggg 7801 atcGCCACCA TGGACAAGAA GTACAGCATC GGCCTGGCCA TCGGCACCAA CTCTGTGGGC 7861 TGGGCCGTGA TCACCGACGA GTACAAGGTG CCCAGCAAGA AATTCAAGGT GCTGGGCAAC 7921 ACCGACCGGC ACAGCATCAA GAAGAACCTG ATCGGCGCCC TGCTGTTCGA CAGCGGAGAA 7981 ACAGCCGAGG CCACCCGGCT GAAGAGAACC GCCAGAAGAA GATACACCAG ACGGAAGAAC 8041 CGGATCTGCT ATCTGCAAGA GATCTTCAGC AACGAGATGG CCAAGGTGGA CGACAGCTTC 8101 TTCCACAGAC TGGAAGAGTC CTTCCTGGTG GAAGAGGATA AGAAGCACGA GCGGCACCCC 8161 ATCTTCGGCA ACATCGTGGA CGAGGTGGCC TACCACGAGA AGTACCCCAC CATCTACCAC 8221 CTGAGAAAGA AACTGGTGGA CAGCACCGAC AAGGCCGACC TGCGGCTGAT CTATCTGGCC 8281 CTGGCCCACA TGATCAAGTT CCGGGGCCAC TTCCTGATCG AGGGCGACCT GAACCCCGAC 8341 AACAGCGACG TGGACAAGCT GTTCATCCAG CTGGTGCAGA CCTACAACCA GCTGTTCGAG 8401 GAAAACCCCA TCAACGCCAG CGGCGTGGAC GCCAAGGCCA TCCTGTCTGC CAGACTGAGC 8461 AAGAGCAGAC GGCTGGAAAA TCTGATCGCC CAGCTGCCCG GCGAGAAGAA GAATGGCCTG 8521 TTCGGCAACC TGATTGCCCT GAGCCTGGGC CTGACCCCCA ACTTCAAGAG CAACTTCGAC 8581 CTGGCCGAGG ATGCCAAACT GCAGCTGAGC AAGGACACCT ACGACGACGA CCTGGACAAC 8641 CTGCTGGCCC AGATCGGCGA CCAGTACGCC GACCTGTTTC TGGCCGCCAA GAACCTGTCC 8701 GACGCCATCC TGCTGAGCGA CATCCTGAGA GTGAACACCG AGATCACCAA GGCCCCCCTG 8761 AGCGCCTCTA TGATCAAGAG ATACGACGAG CACCACCAGG ACCTGACCCT GCTGAAAGCT 8821 CTCGTGCGGC AGCAGCTGCC TGAGAAGTAC AAAGAGATTT TCTTCGACCA GAGCAAGAAC 8881 GGCTACGCCG GCTACATCGA TGGCGGAGCC AGCCAGGAAG AGTTCTACAA GTTCATCAAG 8941 CCCATCCTGG AAAAGATGGA CGGCACCGAG GAACTGCTCG TGAAGCTGAA CAGAGAGGAC 9001 CTGCTGCGGA AGCAGCGGAC CTTCGACAAC GGCAGCATCC CCCACCAGAT CCACCTGGGA 9061 GAGCTGCACG CCATTCTGCG GCGGCAGGAA GATTTTTACC CATTCCTGAA GGACAACCGG 9121 GAAAAGATCG AGAAGATCCT GACCTTCCGC ATCCCCTACT ACGTGGGCCC TCTGGCCAGG 9181 GGAAACAGCA GATTCGCCTG GATGACCAGA AAGAGCGAGG AAACCATCAC CCCCTGGAAC 9241 TTCGAGGAAG TGGTGGACAA GGGCGCCAGC GCCCAGAGCT TCATCGAGCG GATGACCAAC 9301 TTCGATAAGA ACCTGCCCAA CGAGAAGGTG CTGCCCAAGC ACAGCCTGCT GTACGAGTAC 9361 TTCACCGTGT ACAACGAGCT GACCAAAGTG AAATACGTGA CCGAGGGAAT GAGAAAGCCC 9421 GCCTTCCTGA GCGGCGAGCA GAAAAAAGCC ATCGTGGACC TGCTGTTCAA GACCAACCGG 9481 AAAGTGACCG TGAAGCAGCT GAAAGAGGAC TACTTCAAGA AAATCGAGTG CTTCGACTCC 9541 GTGGAAATCT CCGGCGTGGA AGATCGGTTC AACGCCTCCC TGGGCACATA CCACGATCTG 9601 CTGAAAATTA TCAAGGACAA GGACTTCCTG GACAATGAGG AAAACGAGGA CATTCTGGAA 9661 GATATCGTGC TGACCCTGAC ACTGTTTGAG GACAGAGAGA TGATCGAGGA ACGGCTGAAA 9721 ACCTATGCCC ACCTGTTCGA CGACAAAGTG ATGAAGCAGC TGAAGCGGCG GAGATACACC 9781 GGCTGGGGCA GGCTGAGCCG GAAGCTGATC AACGGCATCC GGGACAAGCA GTCCGGCAAG 9841 ACAATCCTGG ATTTCCTGAA GTCCGACGGC TTCGCCAACA GAAACTTCAT GCAGCTGATC 9901 CACGACGACA GCCTGACCTT TAAAGAGGAC ATCCAGAAAG CCCAGGTGTC CGGCCAGGGC 9961 GATAGCCTGC ACGAGCACAT TGCCAATCTG GCCGGCAGCC CCGCCATTAA GAAGGGCATC 10021 CTGCAGACAG TGAAGGTGGT GGACGAGCTC GTGAAAGTGA TGGGCCGGCA CAAGCCCGAG 10081 AACATCGTGA TCGAAATGGC CAGAGAGAAC CAGACCACCC AGAAGGGACA GAAGAACAGC 10141 CGCGAGAGAA TGAAGCGGAT CGAAGAGGGC ATCAAAGAGC TGGGCAGCCA GATCCTGAAA 10201 GAACACCCCG TGGAAAACAC CCAGCTGCAG AACGAGAAGC TGTACCTGTA CTACCTGCAG 10261 AATGGGCGGG ATATGTACGT GGACCAGGAA CTGGACATCA ACCGGCTGTC CGACTACGAT 10321 GTGGACGCTA TCGTGCCTCA GAGCTTTCTG AAGGACGACT CCATCGATAA CAAAGTGCTG 10381 ACTCGGAGCG ACAAGAACCG GGGCAAGAGC GACAACGTGC CCTCCGAAGA GGTCGTGAAG 10441 AAGATGAAGA ACTACTGGCG CCAGCTGCTG AATGCCAAGC TGATTACCCA GAGGAAGTTC 10501 GACAATCTGA CCAAGGCCGA GAGAGGCGGC CTGAGCGAAC TGGATAAGGC CGGCTTCATC 10561 AAGAGACAGC TGGTGGAAAC CCGGCAGATC ACAAAGCACG TGGCACAGAT CCTGGACTCC 10621 CGGATGAACA CTAAGTACGA CGAGAACGAC AAACTGATCC GGGAAGTGAA AGTGATCACC 10681 CTGAAGTCCA AGCTGGTGTC CGATTTCCGG AAGGATTTCC AGTTTTACAA AGTGCGCGAG 10741 ATCAACAACT ACCACCACGC CCACGACGCC TACCTGAACG CCGTCGTGGG AACCGCCCTG 10801 ATCAAAAAGT ACCCTAAGCT GGAAAGCGAG TTCGTGTACG GCGACTACAA GGTGTACGAC 10861 GTGCGGAAGA TGATCGCCAA GAGCGAGCAG GAAATCGGCA AGGCTACCGC CAAGTACTTC 10921 TTCTACAGCA ACATCATGAA CTTTTTCAAG ACCGAGATTA CCCTGGCCAA CGGCGAGATC 10981 CGGAAGCGGC CTCTGATCGA GACAAACGGC GAAACAGGCG AGATCGTGTG GGATAAGGGC 11041 CGGGACTTTG CCACCGTGCG GAAAGTGCTG TCTATGCCCC AAGTGAATAT CGTGAAAAAG 11101 ACCGAGGTGC AGACAGGCGG CTTCAGCAAA GAGTCTATCC TGCCCAAGAG GAACAGCGAC 11161 AAGCTGATCG CCAGAAAGAA GGACTGGGAC CCTAAGAAGT ACGGCGGCTT CGACAGCCCC 11221 ACCGTGGCCT ATTCTGTGCT GGTGGTGGCC AAAGTGGAAA AGGGCAAGTC CAAGAAACTG 11281 AAGAGTGTGA AAGAGCTGCT GGGGATCACC ATCATGGAAA GAAGCAGCTT CGAGAAGAAT 11341 CCCATCGACT TTCTGGAAGC CAAGGGCTAC AAAGAAGTGA AAAAGGACCT GATCATCAAG 11401 CTGCCTAAGT ACTCCCTGTT CGAGCTGGAA AACGGCCGGA AGAGAATGCT GGCCTCTGCC 11461 GGCGAACTGC AGAAGGGAAA CGAACTGGCC CTGCCCTCCA AATATGTGAA CTTCCTGTAC 11521 CTGGCCAGCC ACTATGAGAA GCTGAAGGGC TCCCCCGAGG ATAATGAGCA GAAACAGCTG 11581 TTTGTGGAAC AGCACAAACA CTACCTGGAC GAGATCATCG AGCAGATCAG CGAGTTCTCC 11641 AAGAGAGTGA TCCTGGCCGA CGCTAATCTG GACAAGGTGC TGAGCGCCTA CAACAAGCAC 11701 AGAGACAAGC CTATCAGAGA GCAGGCCGAG AATATCATCC ACCTGTTTAC CCTGACCAAT 11761 CTGGGAGCCC CTGCCGCCTT CAAGTACTTT GACACCACCA TCGACCGGAA GAGGTACACC 11821 AGCACCAAAG AGGTGCTGGA CGCCACCCTG ATCCACCAGA GCATCACCGG CCTGTACGAG 11881 ACACGGATCG ACCTGTCTCA GCTGGGAGGC GACGCCTATC CCTATGACGT GCCCGATTAT 11941 GCCAGCCTGG GCAGCGGCTC CCCCAAGAAA AAACGCAAGG TGGAAGATCC TAAGAAAAAG 12001 CGGAAAGTGG ACGGCATTGG TAGTGGGAGC AACGGCAGCA GCGGATCCaa cggtccgGGT 12061 GGATCTGGAG GTGGAGGTTC TGGAGGAGAA GAACTTTTGA GCAAGAATTA TCATCTTGAG 12121 AACGAAGTGG CTCGTCTTAA GAAAGGTTCT GGCAGTGGAG AAGAACTGCT TTCAAAGAAT 12181 TACCACCTGG AAAATGAGGT AGCTAGACTG AAAAAGGGGA GCGGAAGTGG GGAGGAGTTG 12241 CTGAGCAAAA ATTATCATTT GGAGAACGAA GTAGCACGAC TAAAGAAAGG GTCCGGATCG 12301 GGTGAGGAGT TACTCTCGAA AAATTATCAT CTCGAAAACG AAGTGGCTCG GCTAAAAAAG 12361 GGCAGTGGTT CTGGAGAAGA GCTATTATCT AAAAACTACC ACCTCGAAAA TGAGGTGGCA 12421 CGCTTAAAAA AGGGAAGTGG CAGTGGTGAA GAGCTACTAT CCAAGAATTA TCATCTTGAG 12481 AACGAGGTAG CGCGTTTGAA GAAGGGTTCC GGCTCAGGAG AGGAACTGCT CTCGAAGAAC 12541 TATCATCTTG AAAATGAGGT CGCTCGATTA AAAAAGGGAT CGGGCAGTGG TGAGGAACTA 12601 CTTTCAAAGA ATTACCACCT CGAAAACGAA GTAGCTCGAT TAAAGAAAGG TTCAGGGTCG 12661 GGTGAAGAAT TACTGAGTAA AAATTATCAT CTGGAAAATG AGGTAGCGAG ACTAAAAAAG 12721 GGGAGTGGTT CTGGCGAAGA GTTGCTATCG AAAAATTATC ATCTTGAGAA CGAAGTTGCT 12781 AGGCTCAAAA AGGGCTCAGG CTCAGGCGAG GAGTTGCTCT CGAAAAACTA CCACTTGGAA 12841 AATGAGGTCG CGAGGTTGAA AAAGGGGAGC GGGTCGGGCG AGGAGTTATT GAGCAAAAAC 12901 TATCATTTAG AGAACGAAGT CGCGCGCTTA AAGAAAGGCT CGGGCTCGGG CGAAGAACTC 12961 TTATCGAAGA ACTACCACCT CGAAAATGAG GTCGCCAGGT TGAAAAAGGG CAGTGGCAGC 13021 GGGGAGGAAC TCTTGAGCAA GAACTACCAC TTGGAGAATG AGGTCGCGAG ATTGAAGAAA 13081 GGGTCGGGGA GCGGCGAGGA ATTGCTCAGC AAGAATTATC ATTTGGAGAA CGAAGTCGCC 13141 AGGCTCAAGA AAGGCTCGGG GTCGGGGGAG GAATTGTTGA GTAAAAACTA CCACTTGGAA 13201 AATGAAGTCG CCAGGCTCAA AAAAGGGAGT GGGAGCGGCG AAGAGTTATT GAGCAAAAAT 13261 TACCACTTGG AGAACGAAGT GGCAAGGCTC AAGAAAGGGA GCGGCAGCGG GGAGGAGCTC 13321 TTATCGAAGA ACTACCACTT AGAGAATGAA GTCGCCCGCT TGAAGAAAGG CTCGGGGAGC 13381 GGGGAAGAGC TCTTGAGCAA GAACTACCAC TTGGAAAATG AGGTGGCGCG CTTGAAGAAA 13441 GGGAGCGGGA GCGGGGAAGA GTTACTATCT AAGAATTATC ATCTCGAGAA CGAGGTGGCT 13501 CGACTAAAGA AGGGCTCCGG CAGTGGGGAG GAACTCCTGT CGAAGAACTA TCATCTTGAA 13561 AATGAGGTTG CAAGACTTAA AAAGGGGTCC GGATCAGGTG AGGAACTACT CAGTAAGAAT 13621 TACCACCTGG AAAACGAAGT TGCACGTTTG AAGAAAGGAT CAGGATCAGG CGAAGAACTG 13681 CTCTCAAAAG ATTATCATTT GGAAAATGAG GTTGCACGTT TAAAAAAGGG AAGTGGCAGT 13741 GGTGAGGAAC TTCTGTCGAA AAATTATCAT CTCGAGAATG AAGTAGCCCG ACTTAAAAAG 13801 GGTTCTGGCT CGGGTcagcg gccgcaaGGT GGAGGTGGAC CCAAGAAGAA GCGCAAGGTG 13861 GGAAGCGGAG CTACTAACTT CAGCCTGCTG AAGCAGGCTG GAGACGTGGA GGAGAACCCT 13921 GGACCTGCAC CAGGAAGTAT GAGCGAGCTG ATTAAGGAGA ACATGCACAT GAAGCTGTAC 13981 ATGGAGGGCA CCGTGGACAA CCATCACTTC AAGTGCACAT CCGAGGGCGA AGGCAAGCCC 14041 TACGAGGGCA CCCAGACCAT GAGAATCAAG GTGGTCGAGG GCGGCCCTCT CCCCTTCGCC 14101 TTCGACATCC TGGCTACTAG CTTCCTCTAC GGCAGCAAGA CCTTCATCAA CCACACCCAG 14161 GGCATCCCCG ACTTCTTCAA GCAGTCCTTC CCTGAGGGCT TCACATGGGA GAGAGTCACC 14221 ACATACGAAG ACGGGGGCGT GCTGACCGCT ACCCAGGACA CCAGCCTCCA GGACGGCTGC 14281 CTCATCTACA ACGTCAAGAT CAGAGGGGTG AACTTCACAT CCAACGGCCC TGTGATGCAG 14341 AAGAAAACAC TCGGCTGGGA GGCCTTCACC GAGACGCTGT ACCCCGCTGA CGGCGGCCTG 14401 GAAGGCAGAA ACGACATGGC CCTGAAGCTC GTGGGCGGGA GCCATCTGAT CGCAAACATC 14461 AAGACCACAT ATAGATCCAA GAAACCCGCT AAGAACCTCA AGATGCCTGG CGTCTACTAT 14521 GTGGACTACA GACTGGAAAG AATCAAGGAG GCCAACAACG AGACCTACGT CGAGCAGCAC 14581 GAGGTGGCAG TGGCCAGATA CTGCGACCTC CCTAGCAAAC TGGGGCACAA GCTTAATTAA 14641 gcggccgcga ctctagagtc gacctgcagg GATCGATCAC TGAggtacct ttaagaccaa 14701 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag 14761 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt 14821 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 14881 aataaagctt gccttgagtg cttcaagtag t //