pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE

价格:1200元

联系方式:I47-825O-882O

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质粒基本信息

质粒名称: pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE 
出品公司: 爱迪基因
目录编号: 60910
存储实验室: Ron Vale

载体骨架基本信息
载体名称: pHR
载体抗性: 氨苄霉素
载体类型: 哺乳细胞表达
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: dCas9
启动子 dSV40
标签/融合蛋白: NLS(N端)       HA(C端)        2x NLS(C端)
克隆方法: 限制性内切酶
5' 克隆位点: 未知
3' 克隆位点: BamHI (未破坏)
5' 测序引物 SV40pro-F2
3' 测序引物 mTagBFP-R

文献引用方法
材料和方法部分: pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE was a gift from Ron Vale (Addgene plasmid # 60910)
参考文献部分: A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging. Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD. Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039. 10.1016/j.cell.2014.09.039 PubMed 25307933

质粒图谱信息

pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE载体图谱

质粒简介

质粒序列

LOCUS       pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE	14911 bp 	DNA	SYN
DEFINITION  pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..14911
                     /organism="pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE"
                     /mol_type="other DNA"
     misc_feature    complement(103..120)
                     /label="Sp6_primer"
     gene            358..621
                     /label="tet (300 - 563)"
                     /gene="tet (300 - 563)"
     misc_feature    complement(410..429)
                     /label="pBRrevBam_primer"
     misc_feature    763..785
                     /label="pGEX_3_primer"
     promoter        944..972
                     /label="AmpR_promoter"
     gene            1014..1874
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             1014..1874
                     /label="ORF frame 3"
     rep_origin      2029..2648
                     /label="pBR322_origin"
     misc_feature    complement(2874..2894)
                     /label="pBABE_3_primer"
     misc_feature    complement(2880..3333)
                     /label="SV40_enhancer"
     promoter        2892..3160
                     /label="SV40_promoter"
     rep_origin      3059..3136
                     /label="SV40_origin"
     misc_feature    3121..3140
                     /label="SV40pro_F_primer"
     misc_feature    4341..4356
                     /label="SV40_int"
     misc_feature    4362..4409
                     /label="SV40_3_splice"
     CDS             4486..4959
                     /label="ORF frame 1"
     misc_feature    4537..4557
                     /label="SV40 NLS"
     misc_feature    4543..4557
                     /label="NLS"
     terminator      4985..5116
                     /label="SV40_PA_terminator"
     misc_feature    5073..5092
                     /label="EBV_rev_primer"
     misc_feature    5738..5918
                     /label="HIV-1_5_LTR"
     misc_feature    5738..5918
                     /label="truncHIV-1_3_LTR"
     misc_feature    6029..6073
                     /label="HIV-1_psi_pack"
     CDS             6460..7557
                     /label="ORF frame 1"
     misc_feature    6582..6815
                     /label="RRE"
     misc_feature    7333..7348
                     /label="cPPT"
     promoter        7531..7710
                     /label="SV40_promoter"
     rep_origin      7578..7655
                     /label="SV40_origin"
     misc_feature    7640..7659
                     /label="SV40pro_F_primer"
     CDS             7732..14640
                     /label="ORF frame 1"
     misc_feature    7735..7758
                     /label="SV40 NLS"
     misc_feature    7735..7755
                     /label="SV40 NLS"
     misc_feature    7741..7755
                     /label="NLS"
     CDS             complement(7774..12114)
                     /label="ORF frame 2"
     misc_feature    11917..11943
                     /label="HA"
     CDS             complement(13720..14445)
                     /label="ORF frame 2"
     misc_feature    14738..14759
                     /label="U3PPT"
     misc_feature    14738..14753
                     /label="cPPT"
     misc_feature    14755..14807
                     /label="delta_U3"
     misc_feature    14808..77
                     /label="HIV-1_5_LTR"
     misc_feature    14808..77
                     /label="truncHIV-1_3_LTR"
ORIGIN
    1 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
   61 gtggaaaatc tctagcagca tctagaatta attccgtgta ttctatagtg tcacctaaat
  121 cgtatgtgta tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg
  181 tacaagccta attgtgtagc atctggctta ctgaagcaga ccctatcatc tctctcgtaa
  241 actgccgtca gagtcggttt ggttggacga accttctgag tttctggtaa cgccgtcccg
  301 cacccggaaa tggtcagcga accaatcagc agggtcatcg ctagccagat cctctacgcc
  361 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
  421 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
  481 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
  541 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
  601 caggagtcgc ataagggaga gcgtcgaatg gtgcactctc agtacaatct agctctgatg
  661 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
  721 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
  781 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
  841 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
  901 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
  961 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
 1021 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
 1081 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
 1141 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
 1201 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
 1261 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
 1321 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
 1381 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
 1441 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
 1501 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
 1561 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
 1621 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
 1681 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
 1741 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
 1801 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
 1861 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
 1921 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
 1981 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
 2041 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
 2101 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
 2161 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
 2221 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
 2281 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
 2341 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
 2401 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
 2461 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
 2521 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
 2581 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
 2641 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
 2701 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
 2761 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
 2821 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt
 2881 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
 2941 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
 3001 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
 3061 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
 3121 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
 3181 cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat
 3241 gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact
 3301 catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc
 3361 cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat
 3421 acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc
 3481 cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt
 3541 actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca
 3601 cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat
 3661 cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc
 3721 aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta
 3781 tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt
 3841 cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct
 3901 ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga
 3961 cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga
 4021 cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta
 4081 aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg
 4141 acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg
 4201 acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg
 4261 ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct
 4321 acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta
 4381 ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag
 4441 tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat
 4501 gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa
 4561 gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat
 4621 agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag
 4681 aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac
 4741 atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa
 4801 aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat
 4861 agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt
 4921 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
 4981 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
 5041 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
 5101 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
 5161 cccataccct attaccactg ccaattacct agtggtttca tttactctaa acctgtgatt
 5221 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
 5281 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
 5341 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
 5401 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
 5461 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
 5521 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
 5581 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
 5641 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
 5701 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
 5761 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
 5821 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
 5881 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
 5941 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
 6001 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
 6061 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
 6121 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
 6181 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
 6241 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
 6301 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
 6361 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
 6421 cagcaagcgg ccggtgatct tcagacctgg acgatatata tgagggacaa ttggagaagt
 6481 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
 6541 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
 6601 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
 6661 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
 6721 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
 6781 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
 6841 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
 6901 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
 6961 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
 7021 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
 7081 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
 7141 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
 7201 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
 7261 agagacagat ccattcgatt agtgaacgga tctcgacggt ATcgccaaat ggcagtattc
 7321 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
 7381 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
 7441 aattttcggg tttattacag ggacagcaga gatccagttt ggatcgataa gcttgatatc
 7501 gaattcaggc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
 7561 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
 7621 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
 7681 tccagaagta gtgaggaggc ttttttggag gcctaggcta cgcgcGCCAC CATGCCCAAG
 7741 AAGAAGCGCA AGGTGGGacg cgtctgcagg atatcaagct tgcggtaccg cgggcccggg
 7801 atcGCCACCA TGGACAAGAA GTACAGCATC GGCCTGGCCA TCGGCACCAA CTCTGTGGGC
 7861 TGGGCCGTGA TCACCGACGA GTACAAGGTG CCCAGCAAGA AATTCAAGGT GCTGGGCAAC
 7921 ACCGACCGGC ACAGCATCAA GAAGAACCTG ATCGGCGCCC TGCTGTTCGA CAGCGGAGAA
 7981 ACAGCCGAGG CCACCCGGCT GAAGAGAACC GCCAGAAGAA GATACACCAG ACGGAAGAAC
 8041 CGGATCTGCT ATCTGCAAGA GATCTTCAGC AACGAGATGG CCAAGGTGGA CGACAGCTTC
 8101 TTCCACAGAC TGGAAGAGTC CTTCCTGGTG GAAGAGGATA AGAAGCACGA GCGGCACCCC
 8161 ATCTTCGGCA ACATCGTGGA CGAGGTGGCC TACCACGAGA AGTACCCCAC CATCTACCAC
 8221 CTGAGAAAGA AACTGGTGGA CAGCACCGAC AAGGCCGACC TGCGGCTGAT CTATCTGGCC
 8281 CTGGCCCACA TGATCAAGTT CCGGGGCCAC TTCCTGATCG AGGGCGACCT GAACCCCGAC
 8341 AACAGCGACG TGGACAAGCT GTTCATCCAG CTGGTGCAGA CCTACAACCA GCTGTTCGAG
 8401 GAAAACCCCA TCAACGCCAG CGGCGTGGAC GCCAAGGCCA TCCTGTCTGC CAGACTGAGC
 8461 AAGAGCAGAC GGCTGGAAAA TCTGATCGCC CAGCTGCCCG GCGAGAAGAA GAATGGCCTG
 8521 TTCGGCAACC TGATTGCCCT GAGCCTGGGC CTGACCCCCA ACTTCAAGAG CAACTTCGAC
 8581 CTGGCCGAGG ATGCCAAACT GCAGCTGAGC AAGGACACCT ACGACGACGA CCTGGACAAC
 8641 CTGCTGGCCC AGATCGGCGA CCAGTACGCC GACCTGTTTC TGGCCGCCAA GAACCTGTCC
 8701 GACGCCATCC TGCTGAGCGA CATCCTGAGA GTGAACACCG AGATCACCAA GGCCCCCCTG
 8761 AGCGCCTCTA TGATCAAGAG ATACGACGAG CACCACCAGG ACCTGACCCT GCTGAAAGCT
 8821 CTCGTGCGGC AGCAGCTGCC TGAGAAGTAC AAAGAGATTT TCTTCGACCA GAGCAAGAAC
 8881 GGCTACGCCG GCTACATCGA TGGCGGAGCC AGCCAGGAAG AGTTCTACAA GTTCATCAAG
 8941 CCCATCCTGG AAAAGATGGA CGGCACCGAG GAACTGCTCG TGAAGCTGAA CAGAGAGGAC
 9001 CTGCTGCGGA AGCAGCGGAC CTTCGACAAC GGCAGCATCC CCCACCAGAT CCACCTGGGA
 9061 GAGCTGCACG CCATTCTGCG GCGGCAGGAA GATTTTTACC CATTCCTGAA GGACAACCGG
 9121 GAAAAGATCG AGAAGATCCT GACCTTCCGC ATCCCCTACT ACGTGGGCCC TCTGGCCAGG
 9181 GGAAACAGCA GATTCGCCTG GATGACCAGA AAGAGCGAGG AAACCATCAC CCCCTGGAAC
 9241 TTCGAGGAAG TGGTGGACAA GGGCGCCAGC GCCCAGAGCT TCATCGAGCG GATGACCAAC
 9301 TTCGATAAGA ACCTGCCCAA CGAGAAGGTG CTGCCCAAGC ACAGCCTGCT GTACGAGTAC
 9361 TTCACCGTGT ACAACGAGCT GACCAAAGTG AAATACGTGA CCGAGGGAAT GAGAAAGCCC
 9421 GCCTTCCTGA GCGGCGAGCA GAAAAAAGCC ATCGTGGACC TGCTGTTCAA GACCAACCGG
 9481 AAAGTGACCG TGAAGCAGCT GAAAGAGGAC TACTTCAAGA AAATCGAGTG CTTCGACTCC
 9541 GTGGAAATCT CCGGCGTGGA AGATCGGTTC AACGCCTCCC TGGGCACATA CCACGATCTG
 9601 CTGAAAATTA TCAAGGACAA GGACTTCCTG GACAATGAGG AAAACGAGGA CATTCTGGAA
 9661 GATATCGTGC TGACCCTGAC ACTGTTTGAG GACAGAGAGA TGATCGAGGA ACGGCTGAAA
 9721 ACCTATGCCC ACCTGTTCGA CGACAAAGTG ATGAAGCAGC TGAAGCGGCG GAGATACACC
 9781 GGCTGGGGCA GGCTGAGCCG GAAGCTGATC AACGGCATCC GGGACAAGCA GTCCGGCAAG
 9841 ACAATCCTGG ATTTCCTGAA GTCCGACGGC TTCGCCAACA GAAACTTCAT GCAGCTGATC
 9901 CACGACGACA GCCTGACCTT TAAAGAGGAC ATCCAGAAAG CCCAGGTGTC CGGCCAGGGC
 9961 GATAGCCTGC ACGAGCACAT TGCCAATCTG GCCGGCAGCC CCGCCATTAA GAAGGGCATC
10021 CTGCAGACAG TGAAGGTGGT GGACGAGCTC GTGAAAGTGA TGGGCCGGCA CAAGCCCGAG
10081 AACATCGTGA TCGAAATGGC CAGAGAGAAC CAGACCACCC AGAAGGGACA GAAGAACAGC
10141 CGCGAGAGAA TGAAGCGGAT CGAAGAGGGC ATCAAAGAGC TGGGCAGCCA GATCCTGAAA
10201 GAACACCCCG TGGAAAACAC CCAGCTGCAG AACGAGAAGC TGTACCTGTA CTACCTGCAG
10261 AATGGGCGGG ATATGTACGT GGACCAGGAA CTGGACATCA ACCGGCTGTC CGACTACGAT
10321 GTGGACGCTA TCGTGCCTCA GAGCTTTCTG AAGGACGACT CCATCGATAA CAAAGTGCTG
10381 ACTCGGAGCG ACAAGAACCG GGGCAAGAGC GACAACGTGC CCTCCGAAGA GGTCGTGAAG
10441 AAGATGAAGA ACTACTGGCG CCAGCTGCTG AATGCCAAGC TGATTACCCA GAGGAAGTTC
10501 GACAATCTGA CCAAGGCCGA GAGAGGCGGC CTGAGCGAAC TGGATAAGGC CGGCTTCATC
10561 AAGAGACAGC TGGTGGAAAC CCGGCAGATC ACAAAGCACG TGGCACAGAT CCTGGACTCC
10621 CGGATGAACA CTAAGTACGA CGAGAACGAC AAACTGATCC GGGAAGTGAA AGTGATCACC
10681 CTGAAGTCCA AGCTGGTGTC CGATTTCCGG AAGGATTTCC AGTTTTACAA AGTGCGCGAG
10741 ATCAACAACT ACCACCACGC CCACGACGCC TACCTGAACG CCGTCGTGGG AACCGCCCTG
10801 ATCAAAAAGT ACCCTAAGCT GGAAAGCGAG TTCGTGTACG GCGACTACAA GGTGTACGAC
10861 GTGCGGAAGA TGATCGCCAA GAGCGAGCAG GAAATCGGCA AGGCTACCGC CAAGTACTTC
10921 TTCTACAGCA ACATCATGAA CTTTTTCAAG ACCGAGATTA CCCTGGCCAA CGGCGAGATC
10981 CGGAAGCGGC CTCTGATCGA GACAAACGGC GAAACAGGCG AGATCGTGTG GGATAAGGGC
11041 CGGGACTTTG CCACCGTGCG GAAAGTGCTG TCTATGCCCC AAGTGAATAT CGTGAAAAAG
11101 ACCGAGGTGC AGACAGGCGG CTTCAGCAAA GAGTCTATCC TGCCCAAGAG GAACAGCGAC
11161 AAGCTGATCG CCAGAAAGAA GGACTGGGAC CCTAAGAAGT ACGGCGGCTT CGACAGCCCC
11221 ACCGTGGCCT ATTCTGTGCT GGTGGTGGCC AAAGTGGAAA AGGGCAAGTC CAAGAAACTG
11281 AAGAGTGTGA AAGAGCTGCT GGGGATCACC ATCATGGAAA GAAGCAGCTT CGAGAAGAAT
11341 CCCATCGACT TTCTGGAAGC CAAGGGCTAC AAAGAAGTGA AAAAGGACCT GATCATCAAG
11401 CTGCCTAAGT ACTCCCTGTT CGAGCTGGAA AACGGCCGGA AGAGAATGCT GGCCTCTGCC
11461 GGCGAACTGC AGAAGGGAAA CGAACTGGCC CTGCCCTCCA AATATGTGAA CTTCCTGTAC
11521 CTGGCCAGCC ACTATGAGAA GCTGAAGGGC TCCCCCGAGG ATAATGAGCA GAAACAGCTG
11581 TTTGTGGAAC AGCACAAACA CTACCTGGAC GAGATCATCG AGCAGATCAG CGAGTTCTCC
11641 AAGAGAGTGA TCCTGGCCGA CGCTAATCTG GACAAGGTGC TGAGCGCCTA CAACAAGCAC
11701 AGAGACAAGC CTATCAGAGA GCAGGCCGAG AATATCATCC ACCTGTTTAC CCTGACCAAT
11761 CTGGGAGCCC CTGCCGCCTT CAAGTACTTT GACACCACCA TCGACCGGAA GAGGTACACC
11821 AGCACCAAAG AGGTGCTGGA CGCCACCCTG ATCCACCAGA GCATCACCGG CCTGTACGAG
11881 ACACGGATCG ACCTGTCTCA GCTGGGAGGC GACGCCTATC CCTATGACGT GCCCGATTAT
11941 GCCAGCCTGG GCAGCGGCTC CCCCAAGAAA AAACGCAAGG TGGAAGATCC TAAGAAAAAG
12001 CGGAAAGTGG ACGGCATTGG TAGTGGGAGC AACGGCAGCA GCGGATCCaa cggtccgGGT
12061 GGATCTGGAG GTGGAGGTTC TGGAGGAGAA GAACTTTTGA GCAAGAATTA TCATCTTGAG
12121 AACGAAGTGG CTCGTCTTAA GAAAGGTTCT GGCAGTGGAG AAGAACTGCT TTCAAAGAAT
12181 TACCACCTGG AAAATGAGGT AGCTAGACTG AAAAAGGGGA GCGGAAGTGG GGAGGAGTTG
12241 CTGAGCAAAA ATTATCATTT GGAGAACGAA GTAGCACGAC TAAAGAAAGG GTCCGGATCG
12301 GGTGAGGAGT TACTCTCGAA AAATTATCAT CTCGAAAACG AAGTGGCTCG GCTAAAAAAG
12361 GGCAGTGGTT CTGGAGAAGA GCTATTATCT AAAAACTACC ACCTCGAAAA TGAGGTGGCA
12421 CGCTTAAAAA AGGGAAGTGG CAGTGGTGAA GAGCTACTAT CCAAGAATTA TCATCTTGAG
12481 AACGAGGTAG CGCGTTTGAA GAAGGGTTCC GGCTCAGGAG AGGAACTGCT CTCGAAGAAC
12541 TATCATCTTG AAAATGAGGT CGCTCGATTA AAAAAGGGAT CGGGCAGTGG TGAGGAACTA
12601 CTTTCAAAGA ATTACCACCT CGAAAACGAA GTAGCTCGAT TAAAGAAAGG TTCAGGGTCG
12661 GGTGAAGAAT TACTGAGTAA AAATTATCAT CTGGAAAATG AGGTAGCGAG ACTAAAAAAG
12721 GGGAGTGGTT CTGGCGAAGA GTTGCTATCG AAAAATTATC ATCTTGAGAA CGAAGTTGCT
12781 AGGCTCAAAA AGGGCTCAGG CTCAGGCGAG GAGTTGCTCT CGAAAAACTA CCACTTGGAA
12841 AATGAGGTCG CGAGGTTGAA AAAGGGGAGC GGGTCGGGCG AGGAGTTATT GAGCAAAAAC
12901 TATCATTTAG AGAACGAAGT CGCGCGCTTA AAGAAAGGCT CGGGCTCGGG CGAAGAACTC
12961 TTATCGAAGA ACTACCACCT CGAAAATGAG GTCGCCAGGT TGAAAAAGGG CAGTGGCAGC
13021 GGGGAGGAAC TCTTGAGCAA GAACTACCAC TTGGAGAATG AGGTCGCGAG ATTGAAGAAA
13081 GGGTCGGGGA GCGGCGAGGA ATTGCTCAGC AAGAATTATC ATTTGGAGAA CGAAGTCGCC
13141 AGGCTCAAGA AAGGCTCGGG GTCGGGGGAG GAATTGTTGA GTAAAAACTA CCACTTGGAA
13201 AATGAAGTCG CCAGGCTCAA AAAAGGGAGT GGGAGCGGCG AAGAGTTATT GAGCAAAAAT
13261 TACCACTTGG AGAACGAAGT GGCAAGGCTC AAGAAAGGGA GCGGCAGCGG GGAGGAGCTC
13321 TTATCGAAGA ACTACCACTT AGAGAATGAA GTCGCCCGCT TGAAGAAAGG CTCGGGGAGC
13381 GGGGAAGAGC TCTTGAGCAA GAACTACCAC TTGGAAAATG AGGTGGCGCG CTTGAAGAAA
13441 GGGAGCGGGA GCGGGGAAGA GTTACTATCT AAGAATTATC ATCTCGAGAA CGAGGTGGCT
13501 CGACTAAAGA AGGGCTCCGG CAGTGGGGAG GAACTCCTGT CGAAGAACTA TCATCTTGAA
13561 AATGAGGTTG CAAGACTTAA AAAGGGGTCC GGATCAGGTG AGGAACTACT CAGTAAGAAT
13621 TACCACCTGG AAAACGAAGT TGCACGTTTG AAGAAAGGAT CAGGATCAGG CGAAGAACTG
13681 CTCTCAAAAG ATTATCATTT GGAAAATGAG GTTGCACGTT TAAAAAAGGG AAGTGGCAGT
13741 GGTGAGGAAC TTCTGTCGAA AAATTATCAT CTCGAGAATG AAGTAGCCCG ACTTAAAAAG
13801 GGTTCTGGCT CGGGTcagcg gccgcaaGGT GGAGGTGGAC CCAAGAAGAA GCGCAAGGTG
13861 GGAAGCGGAG CTACTAACTT CAGCCTGCTG AAGCAGGCTG GAGACGTGGA GGAGAACCCT
13921 GGACCTGCAC CAGGAAGTAT GAGCGAGCTG ATTAAGGAGA ACATGCACAT GAAGCTGTAC
13981 ATGGAGGGCA CCGTGGACAA CCATCACTTC AAGTGCACAT CCGAGGGCGA AGGCAAGCCC
14041 TACGAGGGCA CCCAGACCAT GAGAATCAAG GTGGTCGAGG GCGGCCCTCT CCCCTTCGCC
14101 TTCGACATCC TGGCTACTAG CTTCCTCTAC GGCAGCAAGA CCTTCATCAA CCACACCCAG
14161 GGCATCCCCG ACTTCTTCAA GCAGTCCTTC CCTGAGGGCT TCACATGGGA GAGAGTCACC
14221 ACATACGAAG ACGGGGGCGT GCTGACCGCT ACCCAGGACA CCAGCCTCCA GGACGGCTGC
14281 CTCATCTACA ACGTCAAGAT CAGAGGGGTG AACTTCACAT CCAACGGCCC TGTGATGCAG
14341 AAGAAAACAC TCGGCTGGGA GGCCTTCACC GAGACGCTGT ACCCCGCTGA CGGCGGCCTG
14401 GAAGGCAGAA ACGACATGGC CCTGAAGCTC GTGGGCGGGA GCCATCTGAT CGCAAACATC
14461 AAGACCACAT ATAGATCCAA GAAACCCGCT AAGAACCTCA AGATGCCTGG CGTCTACTAT
14521 GTGGACTACA GACTGGAAAG AATCAAGGAG GCCAACAACG AGACCTACGT CGAGCAGCAC
14581 GAGGTGGCAG TGGCCAGATA CTGCGACCTC CCTAGCAAAC TGGGGCACAA GCTTAATTAA
14641 gcggccgcga ctctagagtc gacctgcagg GATCGATCAC TGAggtacct ttaagaccaa
14701 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
14761 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
14821 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
14881 aataaagctt gccttgagtg cttcaagtag t
//

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