质粒名称: | pMD2.G |
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出品公司: | 爱迪基因 |
目录编号: | 12259 |
存储实验室: | Didier Trono |
载体骨架基本信息 |
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载体名称: | pMD2.G |
载体抗性: | 氨苄霉素 |
空载体大小: | 5824 bp |
载体类型: | 哺乳细胞表达,慢病毒,抗原 |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | VSV G |
克隆方法: | 限制性内切酶 |
5' 测序引物 | CMV-F |
文献引用方法 |
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材料和方法部分: | pMD2.G was a gift from Didier Trono (Addgene plasmid # 12259) |
pMD2.G
价格:1200元
联系方式:I47-825O-882O
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质粒基本信息
质粒图谱信息
质粒简介
质粒序列
LOCUS pMD2.G Sequencing Result 5822 bp DNA SYN DEFINITION pMD2.G Sequencing Result ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5822 /organism="pMD2.G Sequencing Result" /mol_type="other DNA" gene 8..835 /label="vsv-G (696 - 1523)" /gene="vsv-G (696 - 1523)" CDS 20..847 /label="ORF frame 2" misc_feature 812..844 /label="VSVG Tag" gene complement(2711..3571) /label="Ampicillin" /gene="Ampicillin" CDS complement(2711..3571) /label="ORF frame 2" promoter complement(3613..3641) /label="AmpR_promoter" misc_feature complement(3746..3765) /label="SV40pro_F_primer" promoter 3868..4444 /label="CMV_immearly_promoter" misc_feature 3947..4234 /label="CAG_enhancer" misc_feature 4401..4421 /label="CMV_fwd_primer" promoter 4402..4471 /label="CMV_promoter" promoter 4414..4533 /label="CMV2_promoter" misc_feature 4445..4464 /label="pCEP_fwd_primer" misc_feature 4447..4471 /label="LNCX_primer" misc_feature 4721..5104 /label="bGlob_int" gene 5134..835 /label="vsv-G" /gene="vsv-G" CDS 5134..847 /label="ORF frame 1" ORIGIN 1 TGGAGGCAAG GCCTGCAAAA TGCAATACTG CAAGCATTGG GGAGTCAGAC TCCCATCAGG 61 TGTCTGGTTC GAGATGGCTG ATAAGGATCT CTTTGCTGCA GCCAGATTCC CTGAATGCCC 121 AGAAGGGTCA AGTATCTCTG CTCCATCTCA GACCTCAGTG GATGTAAGTC TAATTCAGGA 181 CGTTGAGAGG ATCTTGGATT ATTCCCTCTG CCAAGAAACC TGGAGCAAAA TCAGAGCGGG 241 TCTTCCAATC TCTCCAGTGG ATCTCAGCTA TCTTGCTCCT AAAAACCCAG GAACCGGTCC 301 TGCTTTCACC ATAATCAATG GTACCCTAAA ATACTTTGAG ACCAGATACA TCAGAGTCGA 361 TATTGCTGCT CCAATCCTCT CAAGAATGGT CGGAATGATC AGTGGAACTA CCACAGAAAG 421 GGAACTGTGG GATGACTGGG CACCATATGA AGACGTGGAA ATTGGACCCA ATGGAGTTCT 481 GAGGACCAGT TCAGGATATA AGTTTCCTTT ATACATGATT GGACATGGTA TGTTGGACTC 541 CGATCTTCAT CTTAGCTCAA AGGCTCAGGT GTTCGAACAT CCTCACATTC AAGACGCTGC 601 TTCGCAACTT CCTGATGATG AGAGTTTATT TTTTGGTGAT ACTGGGCTAT CCAAAAATCC 661 AATCGAGCTT GTAGAAGGTT GGTTCAGTAG TTGGAAAAGC TCTATTGCCT CTTTTTTCTT 721 TATCATAGGG TTAATCATTG GACTATTCTT GGTTCTCCGA GTTGGTATCC ATCTTTGCAT 781 TAAATTAAAG CACACCAAGA AAAGACAGAT TTATACAGAC ATAGAGATGA ACCGACTTGG 841 AAAGTAACTC AAATCCTGCA CAACAGATTC TTCATGTTTG GACCAAATCA ACTTGTGATA 901 CCATGCTCAA AGAGGCCTCA ATTATATTTG AGTTTTTAAT TTTTATGAAA AAAAAAAAAA 961 AAAACGGAAT TCACCCCACC AGTGCAGGCT GCCTATCAGA AAGTGGTGGC TGGTGTGGCT 1021 AATGCCCTGG CCCACAAGTA TCACTAAGCT CGCTTTCTTG CTGTCCAATT TCTATTAAAG 1081 GTTCCTTTGT TCCCTAAGTC CAACTACTAA ACTGGGGGAT ATTATGAAGG GCCTTGAGCA 1141 TCTGGATTCT GCCTAATAAA AAACATTTAT TTTCATTGCA ATGATGTATT TAAATTATTT 1201 CTGAATATTT TACTAAAAAG GGAATGTGGG AGGTCAGTGC ATTTAAAACA TAAAGAAATG 1261 AAGAGCTAGT TCAAACCTTG GGAAAATACA CTATATCTTA AACTCCATGA AAGAAGGTGA 1321 GGCTGCAAAC AGCTAATGCA CATTGGCAAC AGCCCCTGAT GCCTATGCCT TATTCATCCC 1381 TCAGAAAAGG ATTCAAGTAG AGGCTTGATT TGGAGGTTAA AGTTTTGCTA TGCTGTATTT 1441 TACATTACTT ATTGTTTTAG CTGTCCTCAT GAATGTCTTT TCACTACCCA TTTGCTTATC 1501 CTGCATCTCT CAGCCTTGAC TCCACTCAGT TCTCTTGCTT AGAGATACCA CCTTTCCCCT 1561 GAAGTGTTCC TTCCATGTTT TACGGCGAGA TGGTTTCTCC TCGCCTGGCC ACTCAGCCTT 1621 AGTTGTCTCT GTTGTCTTAT AGAGGTCTAC TTGAAGAAGG AAAAACAGGG GGCATGGTTT 1681 GACTGTCCTG TGAGCCCTTC TTCCCTGCCT CCCCCACTCA CAGTGACCCG GAATCCCTCG 1741 ACATGGCAGT CTAGCACTAG TGCGGCCGCA GATCTGCTTC CTCGCTCACT GACTCGCTGC 1801 GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT 1861 CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA 1921 GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC 1981 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC 2041 AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG 2101 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA 2161 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG 2221 TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC 2281 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG 2341 GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT 2401 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT 2461 CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC 2521 GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT 2581 GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT 2641 AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT 2701 GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC 2761 GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC 2821 CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT 2881 CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG 2941 CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA 3001 GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA 3061 TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT 3121 GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG 3181 TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA 3241 GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC 3301 GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT 3361 TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC 3421 TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA 3481 CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA 3541 TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA 3601 TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC 3661 AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTGGAT CCCCTGAGGG 3721 GGCCCCCATG GGCTAGAGGA TCCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG 3781 CCATGAGCTT GGCCCATTGC ATACGTTGTA TCCATATCAT AATATGTACA TTTATATTGG 3841 CTCATGTCCA ACATTACCGC CATGTTGACA TTGATTATTG ACTAGTTATT AATAGTAATC 3901 AATTACGGGG TCATTAGTTC ATAGCCCATA TATGGAGTTC CGCGTTACAT AACTTACGGT 3961 AAATGGCCCG CCTGGCTGAC CGCCCAACGA CCCCCGCCCA TTGACGTCAA TAATGACGTA 4021 TGTTCCCATA GTAACGCCAA TAGGGACTTT CCATTGACGT CAATGGGTGG AGTATTTACG 4081 GTAAACTGCC CACTTGGCAG TACATCAAGT GTATCATATG CCAAGTACGC CCCCTATTGA 4141 CGTCAATGAC GGTAAATGGC CCGCCTGGCA TTATGCCCAG TACATGACCT TATGGGACTT 4201 TCCTACTTGG CAGTACATCT ACGTATTAGT CATCGCTATT ACCATGGTGA TGCGGTTTTG 4261 GCAGTACATC AATGGGCGTG GATAGCGGTT TGACTCACGG GGATTTCCAA GTCTCCACCC 4321 CATTGACGTC AATGGGAGTT TGTTTTGGCA CCAAAATCAA CGGGACTTTC CAAAATGTCG 4381 TAACAACTCC GCCCCATTGA CGCAAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT 4441 AAGCAGAGCT CGTTTAGTGA ACCGTCAGAT CGCCTGGAGA CGCCATCCAC GCTGTTTTGA 4501 CCTCCATAGA AGACACCGGG ACCGATCCAG CCTCCCCTCG AAGCTTACAT GTGGTACCGA 4561 GCTCGGATCC TGAGAACTTC AGGGTGAGTC TATGGGACCC TTGATGTTTT CTTTCCCCTT 4621 CTTTTCTATG GTTAAGTTCA TGTCATAGGA AGGGGAGAAG TAACAGGGTA CACATATTGA 4681 CCAAATCAGG GTAATTTTGC ATTTGTAATT TTAAAAAATG CTTTCTTCTT TTAATATACT 4741 TTTTTGTTTA TCTTATTTCT AATACTTTCC CTAATCTCTT TCTTTCAGGG CAATAATGAT 4801 ACAATGTATC ATGCCTCTTT GCACCATTCT AAAGAATAAC AGTGATAATT TCTGGGTTAA 4861 GGCAATAGCA ATATTTCTGC ATATAAATAT TTCTGCATAT AAATTGTAAC TGATGTAAGA 4921 GGTTTCATAT TGCTAATAGC AGCTACAATC CAGCTACCAT TCTGCTTTTA TTTTATGGTT 4981 GGGATAAGGC TGGATTATTC TGAGTCCAAG CTAGGCCCTT TTGCTAATCA TGTTCATACC 5041 TCTTATCTTC CTCCCACAGC TCCTGGGCAA CGTGCTGGTC TGTGTGCTGG CCCATCACTT 5101 TGGCAAAGCA CGTGAGATCT GAATTCTGAC ACTATGAAGT GCCTTTTGTA CTTAGCCTTT 5161 TTATTCATTG GGGTGAATTG CAAGTTCACC ATAGTTTTTC CACACAACCA AAAAGGAAAC 5221 TGGAAAAATG TTCCTTCTAA TTACCATTAT TGCCCGTCAA GCTCAGATTT AAATTGGCAT 5281 AATGACTTAA TAGGCACAGC CTTACAAGTC AAAATGCCCA AGAGTCACAA GGCTATTCAA 5341 GCAGACGGTT GGATGTGTCA TGCTTCCAAA TGGGTCACTA CTTGTGATTT CCGCTGGTAT 5401 GGACCGAAGT ATATAACACA TTCCATCCGA TCCTTCACTC CATCTGTAGA ACAATGCAAG 5461 GAAAGCATTG AACAAACGAA ACAAGGAACT TGGCTGAATC CAGGCTTCCC TCCTCAAAGT 5521 TGTGGATATG CAACTGTGAC GGATGCCGAA GCAGTGATTG TCCAGGTGAC TCCTCACCAT 5581 GTGCTGGTTG ATGAATACAC AGGAGAATGG GTTGATTCAC AGTTCATCAA CGGAAAATGC 5641 AGCAATTACA TATGCCCCAC TGTCCATAAC TCTACAACCT GGCATTCTGA CTATAAGGTC 5701 AAAGGGCTAT GTGATTCTAA CCTCATTTCC ATGGACATCA CCTTCTTCTC AGAGGACGGA 5761 GAGCTATCAT CCCTGGGAAA GGAGGGCACA GGGTTCAGAA GTAACTACTT TGCTTATGAA 5821 AC //
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