Tet-O-FUW-Neurod1

价格:1200元

联系方式:I47-825O-882O

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质粒基本信息

质粒名称: Tet-O-FUW-Neurod1 
出品公司: 爱迪基因
目录编号: 30129
存储实验室: Marius Wernig

载体骨架基本信息
载体名称: Tet-O-FUW
载体抗性: 氨苄霉素
空载体大小: 8373 bp
载体类型: 哺乳细胞表达
筛选标记: 新霉素G418
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: NeuroD1
物种来源: M. musculus (鼠)
插入大小: 1074 bp
克隆方法: 限制性内切酶
5' 克隆位点: EcoR1(未破坏)
3' 克隆位点: EcoR1(未破坏)
5' 测序引物 LNCX
3' 测序引物 WPRE-R

文献引用方法
材料和方法部分: Tet-O-FUW-Neurod1 was a gift from Marius Wernig (Addgene plasmid # 30129)
参考文献部分: Direct conversion of fibroblasts to functional neurons by defined factors. Vierbuchen T, Ostermeier A, Pang ZP, Kokubu Y, Sudhof TC, Wernig M. Nature. 2010 Feb 25. 463(7284):1035-41. 10.1038/nature08797 PubMed 20107439

质粒图谱信息

Tet-O-FUW-Neurod1载体图谱

质粒简介

质粒序列

LOCUS       Tet-O-FUW-Neurod1 Sequencing Result	9471 bp 	DNA	SYN
DEFINITION  Tet-O-FUW-Neurod1 Sequencing Result
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..9471
                     /organism="Tet-O-FUW-Neurod1 Sequencing Result"
                     /mol_type="other DNA"
     promoter        8..584
                     /label="CMV_immearly_promoter"
     misc_feature    87..374
                     /label="CAG_enhancer"
     misc_feature    541..561
                     /label="CMV_fwd_primer"
     misc_feature    604..784
                     /label="truncHIV-1_3_LTR"
     misc_feature    604..784
                     /label="HIV-1_5_LTR"
     misc_feature    895..939
                     /label="HIV-1_psi_pack"
     CDS             1327..2214
                     /label="ORF frame 1"
     misc_feature    1449..1682
                     /label="RRE"
     misc_feature    2213..2228
                     /label="cPPT"
     misc_feature    2369..2686
                     /label="TRE"
     promoter        2688..2807
                     /label="CMV2_promoter"
     misc_feature    2719..2738
                     /label="pCEP_fwd_primer"
     misc_feature    2721..2745
                     /label="LNCX_primer"
     CDS             2834..3907
                     /label="ORF frame 2"
     misc_feature    3934..4521
                     /label="WPRE"
     CDS             4022..5071
                     /label="ORF frame 2"
     CDS             4035..4547
                     /label="ORF frame 3"
     misc_feature    complement(4524..4540)
                     /label="pBluescriptKS_primer"
     misc_feature    4708..4729
                     /label="U3PPT"
     misc_feature    4708..4723
                     /label="cPPT"
     misc_feature    5045..5225
                     /label="truncHIV-1_3_LTR"
     misc_feature    5045..5225
                     /label="HIV-1_5_LTR"
     misc_feature    complement(5251..5268)
                     /label="BGH_rev_primer"
     terminator      5254..5481
                     /label="bGH_PA_terminator"
     rep_origin      5544..5850
                     /label="f1_origin"
     misc_feature    complement(5964..5984)
                     /label="pBABE_3_primer"
     misc_feature    complement(5970..6185)
                     /label="SV40_enhancer"
     promoter        5982..6250
                     /label="SV40_promoter"
     rep_origin      6149..6226
                     /label="SV40_origin"
     misc_feature    6211..6230
                     /label="SV40pro_F_primer"
     promoter        6344..6411
                     /label="EM7_promoter"
     gene            6412..6783
                     /label="bleo"
                     /gene="bleo"
     misc_feature    6412..6786
                     /label="sh_ble"
     terminator      6919..7038
                     /label="SV40_PA_terminator"
     misc_feature    7007..7026
                     /label="EBV_rev_primer"
     promoter        complement(7082..7100)
                     /label="M13_reverse_primer"
     misc_feature    complement(7099..7121)
                     /label="M13_pUC_rev_primer"
     promoter        complement(7135..7164)
                     /label="lac_promoter"
     gene            complement(8247..9107)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(8247..9107)
                     /label="ORF frame 2"
     promoter        complement(9149..9177)
                     /label="AmpR_promoter"
ORIGIN
    1 CGCGTTGACA TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC
   61 ATAGCCCATA TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC
  121 CGCCCAACGA CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA
  181 TAGGGACTTT CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG
  241 TACATCAAGT GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC
  301 CCGCCTGGCA TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT
  361 ACGTATTAGT CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG
  421 GATAGCGGTT TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT
  481 TGTTTTGGCA CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA
  541 CGCAAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT
  601 ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC
  661 CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG
  721 TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT
  781 AGCAGTGGCG CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC
  841 GCAGGACTCG GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA
  901 CGCCAAAAAT TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA
  961 TTAAGCGGGG GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA
 1021 AAAAATATAA ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA
 1081 ATCCTGGCCT GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT
 1141 CCCTTCAGAC AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT
 1201 GTGTGCATCA AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG
 1261 AGCAAAACAA AAGTAAGACC ACCGCACAGC AAGCGGCCGC TGATCTTCAG ACCTGGAGGA
 1321 GGAGATATGA GGGACAATTG GAGAAGTGAA TTATATAAAT ATAAAGTAGT AAAAATTGAA
 1381 CCATTAGGAG TAGCACCCAC CAAGGCAAAG AGAAGAGTGG TGCAGAGAGA AAAAAGAGCA
 1441 GTGGGAATAG GAGCTTTGTT CCTTGGGTTC TTGGGAGCAG CAGGAAGCAC TATGGGCGCA
 1501 GCGTCAATGA CGCTGACGGT ACAGGCCAGA CAATTATTGT CTGGTATAGT GCAGCAGCAG
 1561 AACAATTTGC TGAGGGCTAT TGAGGCGCAA CAGCATCTGT TGCAACTCAC AGTCTGGGGC
 1621 ATCAAGCAGC TCCAGGCAAG AATCCTGGCT GTGGAAAGAT ACCTAAAGGA TCAACAGCTC
 1681 CTGGGGATTT GGGGTTGCTC TGGAAAACTC ATTTGCACCA CTGCTGTGCC TTGGAATGCT
 1741 AGTTGGAGTA ATAAATCTCT GGAACAGATT TGGAATCACA CGACCTGGAT GGAGTGGGAC
 1801 AGAGAAATTA ACAATTACAC AAGCTTAATA CACTCCTTAA TTGAAGAATC GCAAAACCAG
 1861 CAAGAAAAGA ATGAACAAGA ATTATTGGAA TTAGATAAAT GGGCAAGTTT GTGGAATTGG
 1921 TTTAACATAA CAAATTGGCT GTGGTATATA AAATTATTCA TAATGATAGT AGGAGGCTTG
 1981 GTAGGTTTAA GAATAGTTTT TGCTGTACTT TCTATAGTGA ATAGAGTTAG GCAGGGATAT
 2041 TCACCATTAT CGTTTCAGAC CCACCTCCCA ACCCCGAGGG GACCCGACAG GCCCGAAGGA
 2101 ATAGAAGAAG AAGGTGGAGA GAGAGACAGA GACAGATCCA TTCGATTAGT GAACGGATCG
 2161 GCACTGCGTG CGCCAATTCT GCAGACAAAT GGCAGTATTC ATCCACAATT TTAAAAGAAA
 2221 AGGGGGGATT GGGGGGTACA GTGCAGGGGA AAGAATAGTA GACATAATAG CAACAGACAT
 2281 ACAAACTAAA GAATTACAAA AACAAATTAC AAAAATTCAA AATTTTCGGG TTTATTACAG
 2341 GGACAGCAGA GATCCAGTTT GGTTAGATCT CGAGTTTACC ACTCCCTATC AGTGATAGAG
 2401 AAAAGTGAAA GTCGAGTTTA CCACTCCCTA TCAGTGATAG AGAAAAGTGA AAGTCGAGTT
 2461 TACCACTCCC TATCAGTGAT AGAGAAAAGT GAAAGTCGAG TTTACCACTC CCTATCAGTG
 2521 ATAGAGAAAA GTGAAAGTCG AGTTTACCAC TCCCTATCAG TGATAGAGAA AAGTGAAAGT
 2581 CGAGTTTACC ACTCCCTATC AGTGATAGAG AAAAGTGAAA GTCGAGTTTA CCACTCCCTA
 2641 TCAGTGATAG AGAAAAGTGA AAGTCGAGCT CGGTACCCGG GTCGAGGTAG GCGTGTACGG
 2701 TGGGAGGCCT ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCGCCTG GAGACGCCAT
 2761 CCACGCTGTT TTGACCTCCA TAGAAGACAC CGGGACCGAT CCAGCCTCCG CGGCCCCGAA
 2821 TTCGCTAGCC ACCATGACCA AATCATACAG CGAGAGCGGG CTGATGGGCG AGCCTCAGCC
 2881 CCAAGGTCCC CCAAGCTGGA CAGATGAGTG TCTCAGTTCT CAGGACGAGG AACACGAGGC
 2941 AGACAAGAAA GAGGACGAGC TTGAAGCCAT GAATGCAGAG GAGGACTCTC TGAGAAACGG
 3001 GGGAGAGGAG GAGGAGGAAG ATGAGGATCT AGAGGAAGAG GAGGAAGAAG AAGAGGAGGA
 3061 GGAGGATCAA AAGCCCAAGA GACGGGGTCC CAAAAAGAAA AAGATGACCA AGGCGCGCCT
 3121 AGAACGTTTT AAATTAAGGC GCATGAAGGC CAACGCCCGC GAGCGGAACC GCATGCACGG
 3181 GCTGAACGCG GCGCTGGACA ACCTGCGCAA GGTGGTACCT TGCTACTCCA AGACCCAGAA
 3241 ACTGTCTAAA ATAGAGACAC TGCGCTTGGC CAAGAACTAC ATCTGGGCTC TGTCAGAGAT
 3301 CCTGCGCTCA GGCAAAAGCC CTGATCTGGT CTCCTTCGTA CAGACGCTCT GCAAAGGTTT
 3361 GTCCCAGCCC ACTACCAATT TGGTCGCCGG CTGCCTGCAG CTCAACCCTC GGACTTTCTT
 3421 GCCTGAGCAG AACCCGGACA TGCCCCCGCA TCTGCCAACC GCCAGCGCTT CCTTCCCGGT
 3481 GCATCCCTAC TCCTACCAGT CCCCTGGACT GCCCAGCCCG CCCTACGGCA CCATGGACAG
 3541 CTCCCACGTC TTCCACGTCA AGCCGCCGCC ACACGCCTAC AGCGCAGCTC TGGAGCCCTT
 3601 CTTTGAAAGC CCCCTAACTG ACTGCACCAG CCCTTCCTTT GACGGACCCC TCAGCCCGCC
 3661 GCTCAGCATC AATGGCAACT TCTCTTTCAA ACACGAACCA TCCGCCGAGT TTGAAAAAAA
 3721 TTATGCCTTT ACCATGCACT ACCCTGCAGC GACGCTGGCA GGGCCCCAAA GCCACGGATC
 3781 AATCTTCTCT TCCGGTGCCG CTGCCCCTCG CTGCGAGATC CCCATAGACA ACATTATGTC
 3841 TTTCGATAGC CATTCGCATC ATGAGCGAGT CATGAGTGCC CAGCTTAATG CCATCTTTCA
 3901 CGATTAGGAA TTCGATATCA AGCTTATCGA TAATCAACCT CTGGATTACA AAATTTGTGA
 3961 AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT
 4021 AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA
 4081 ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT
 4141 GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT
 4201 CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG
 4261 CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC
 4321 GGGGAAATCA TCGTCCTTTC CTTGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG
 4381 GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT
 4441 GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC
 4501 CCTTTGGGCC GCCTCCCCGC ATCGATACCG TCGACCTCGA GACCTAGAAA AACATGGAGC
 4561 AATCACAAGT AGCAATACAG CAGCTACCAA TGCTGATTGT GCCTGGCTAG AAGCACAAGA
 4621 GGAGGAGGAG GTGGGTTTTC CAGTCACACC TCAGGTACCT TTAAGACCAA TGACTTACAA
 4681 GGCAGCTGTA GATCTTAGCC ACTTTTTAAA AGAAAAGGGG GGACTGGAAG GGCTAATTCA
 4741 CTCCCAACGA AGACAAGATA TCCTTGATCT GTGGATCTAC CACACACAAG GCTACTTCCC
 4801 TGATTGGCAG AACTACACAC CAGGGCCAGG GATCAGATAT CCACTGACCT TTGGATGGTG
 4861 CTACAAGCTA GTACCAGTTG AGCAAGAGAA GGTAGAAGAA GCCAATGAAG GAGAGAACAC
 4921 CCGCTTGTTA CACCCTGTGA GCCTGCATGG GATGGATGAC CCGGAGAGAG AAGTATTAGA
 4981 GTGGAGGTTT GACAGCCGCC TAGCATTTCA TCACATGGCC CGAGAGCTGC ATCCGGACTG
 5041 TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG CTCTCTGGCT AACTAGGGAA
 5101 CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT
 5161 GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT TAGTCAGTGT GGAAAATCTC
 5221 TAGCAGGGCC CGTTTAAACC CGCTGATCAG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT
 5281 CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC
 5341 TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG
 5401 GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG
 5461 GGGATGCGGT GGGCTCTATG GCTTCTGAGG CGGAAAGAAC CAGCTGGGGC TCTAGGGGGT
 5521 ATCCCCACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG
 5581 TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC
 5641 TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC
 5701 GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA
 5761 GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA
 5821 ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG
 5881 ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA
 5941 AATTTAACGC GAATTAATTC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG
 6001 CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG
 6061 AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC
 6121 AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA
 6181 TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCTGC
 6241 CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA
 6301 GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAG CACGTGTTGA CAATTAATCA
 6361 TCGGCATAGT ATATCGGCAT AGTATAATAC GACAAGGTGA GGAACTAAAC CATGGCCAAG
 6421 TTGACCAGTG CCGTTCCGGT GCTCACCGCG CGCGACGTCG CCGGAGCGGT CGAGTTCTGG
 6481 ACCGACCGGC TCGGGTTCTC CCGGGACTTC GTGGAGGACG ACTTCGCCGG TGTGGTCCGG
 6541 GACGACGTGA CCCTGTTCAT CAGCGCGGTC CAGGACCAGG TGGTGCCGGA CAACACCCTG
 6601 GCCTGGGTGT GGGTGCGCGG CCTGGACGAG CTGTACGCCG AGTGGTCGGA GGTCGTGTCC
 6661 ACGAACTTCC GGGACGCCTC CGGGCCGGCC ATGACCGAGA TCGGCGAGCA GCCGTGGGGG
 6721 CGGGAGTTCG CCCTGCGCGA CCCGGCCGGC AACTGCGTGC ACTTCGTGGC CGAGGAGCAG
 6781 GACTGACACG TGCTACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC
 6841 GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG
 6901 TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC
 6961 ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA
 7021 CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA
 7081 TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA
 7141 CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA
 7201 ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGcattaa
 7261 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctCTTC CGCTTCCTCG
 7321 CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG
 7381 GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA
 7441 GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC
 7501 CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA
 7561 GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG
 7621 ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT
 7681 CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT
 7741 GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG
 7801 TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC
 7861 AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC
 7921 ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA
 7981 GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC
 8041 AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG
 8101 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA
 8161 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT
 8221 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA
 8281 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG
 8341 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA
 8401 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT
 8461 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT
 8521 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA
 8581 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA
 8641 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA
 8701 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT
 8761 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA
 8821 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG
 8881 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC
 8941 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA
 9001 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT
 9061 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT
 9121 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT
 9181 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC
 9241 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG
 9301 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT
 9361 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC
 9421 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT A

//

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