pMDLg/pRRE

价格:1200元

联系方式:I47-825O-882O

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质粒基本信息

质粒名称: pMDLg/pRRE 
出品公司: 爱迪基因
目录编号: 12251
存储实验室: Didier Trono

载体骨架基本信息
载体名称: pMD
载体抗性: 氨苄霉素
空载体大小: 8895 bp
载体类型: 哺乳细胞表达,慢病毒,包装
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: HIV-1 GAG/POL
克隆方法: 限制性内切酶
5' 测序引物 CMV-F

文献引用方法
材料和方法部分: pMDLg/pRRE was a gift from Didier Trono (Addgene plasmid # 12251)
参考文献部分:
A third-generation lentivirus vector with a conditional packaging system. Dull T, Zufferey R, Kelly M, Mandel RJ, Nguyen M, Trono D, Naldini L. J Virol. 1998 Nov . 72(11):8463-71. PubMed 9765382

质粒图谱信息

pMDLg/pRRE载体图谱

质粒简介

质粒序列

LOCUS       pMDLg/pRRE Sequencing Result	8890 bp 	DNA	SYN
DEFINITION  pMDLg/pRRE Sequencing Result
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..8890
                     /organism="pMDLg/pRRE Sequencing Result"
                     /mol_type="other DNA"
     promoter        23..599
                     /label="CMV_immearly_promoter"
     misc_feature    102..389
                     /label="CAG_enhancer"
     misc_feature    556..576
                     /label="CMV_fwd_primer"
     promoter        557..626
                     /label="CMV_promoter"
     promoter        569..688
                     /label="CMV2_promoter"
     misc_feature    600..619
                     /label="pCEP_fwd_primer"
     misc_feature    602..626
                     /label="LNCX_primer"
     misc_feature    876..1259
                     /label="bGlob_int"
     gene            1298..2800
                     /label="Gag_HIV (variant)"
                     /gene="Gag_HIV (variant)"
     CDS             1298..2800
                     /label="ORF frame 2"
     CDS             2866..5604
                     /label="ORF frame 1"
     misc_feature    5293..5308
                     /label="cPPT"
     CDS             5549..6067
                     /label="ORF frame 2"
     misc_feature    5629..5862
                     /label="RRE"
     gene            complement(7756..8616)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(7756..8616)
                     /label="ORF frame 2"
     promoter        complement(8658..8686)
                     /label="AmpR_promoter"
     misc_feature    complement(8791..8810)
                     /label="SV40pro_F_primer"
ORIGIN
    1 GTCCAACATT ACCGCCATGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA
   61 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG
  121 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC
  181 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA
  241 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA
  301 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA
  361 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT
  421 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG
  481 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA
  541 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA
  601 GAGCTCGTTT AGTGAACCGT CAGATCGCCT GGAGACGCCA TCCACGCTGT TTTGACCTCC
  661 ATAGAAGACA CCGGGACCGA TCCAGCCTCC CCTCGAAGCT TACATGTGGT ACCGAGCTCG
  721 GATCCTGAGA ACTTCAGGGT GAGTCTATGG GACCCTTGAT GTTTTCTTTC CCCTTCTTTT
  781 CTATGGTTAA GTTCATGTCA TAGGAAGGGG AGAAGTAACA GGGTACACAT ATTGACCAAA
  841 TCAGGGTAAT TTTGCATTTG TAATTTTAAA AAATGCTTTC TTCTTTTAAT ATACTTTTTT
  901 GTTTATCTTA TTTCTAATAC TTTCCCTAAT CTCTTTCTTT CAGGGCAATA ATGATACAAT
  961 GTATCATGCC TCTTTGCACC ATTCTAAAGA ATAACAGTGA TAATTTCTGG GTTAAGGCAA
 1021 TAGCAATATT TCTGCATATA AATATTTCTG CATATAAATT GTAACTGATG TAAGAGGTTT
 1081 CATATTGCTA ATAGCAGCTA CAATCCAGCT ACCATTCTGC TTTTATTTTA TGGTTGGGAT
 1141 AAGGCTGGAT TATTCTGAGT CCAAGCTAGG CCCTTTTGCT AATCATGTTC ATACCTCTTA
 1201 TCTTCCTCCC ACAGCTCCTG GGCAACGTGC TGGTCTGTGT GCTGGCCCAT CACTTTGGCA
 1261 AAGCACGTGA GATCTGAATT CGAGATCTGC CGCCGCCATG GGTGCGAGAG CGTCAGTATT
 1321 AAGCGGGGGA GAATTAGATC GATGGGAAAA AATTCGGTTA AGGCCAGGGG GAAAGAAAAA
 1381 ATATAAATTA AAACATATAG TATGGGCAAG CAGGGAGCTA GAACGATTCG CAGTTAATCC
 1441 TGGCCTGTTA GAAACATCAG AAGGCTGTAG ACAAATACTG GGACAGCTAC AACCATCCCT
 1501 TCAGACAGGA TCAGAAGAAC TTAGATCATT ATATAATACA GTAGCAACCC TCTATTGTGT
 1561 GCATCAAAGG ATAGAGATAA AAGACACCAA GGAAGCTTTA GACAAGATAG AGGAAGAGCA
 1621 AAACAAAAGT AAGAAAAAAG CACAGCAAGC AGCAGCTGAC ACAGGACACA GCAATCAGGT
 1681 CAGCCAAAAT TACCCTATAG TGCAGAACAT CCAGGGGCAA ATGGTACATC AGGCCATATC
 1741 ACCTAGAACT TTAAATGCAT GGGTAAAAGT AGTAGAAGAG AAGGCTTTCA GCCCAGAAGT
 1801 GATACCCATG TTTTCAGCAT TATCAGAAGG AGCCACCCCA CAAGATTTAA ACACCATGCT
 1861 AAACACAGTG GGGGGACATC AAGCAGCCAT GCAAATGTTA AAAGAGACCA TCAATGAGGA
 1921 AGCTGCAGAA TGGGATAGAG TGCATCCAGT GCATGCAGGG CCTATTGCAC CAGGCCAGAT
 1981 GAGAGAACCA AGGGGAAGTG ACATAGCAGG AACTACTAGT ACCCTTCAGG AACAAATAGG
 2041 ATGGATGACA CATAATCCAC CTATCCCAGT AGGAGAAATC TATAAAAGAT GGATAATCCT
 2101 GGGATTAAAT AAAATAGTAA GAATGTATAG CCCTACCAGC ATTCTGGACA TAAGACAAGG
 2161 ACCAAAGGAA CCCTTTAGAG ACTATGTAGA CCGATTCTAT AAAACTCTAA GAGCCGAGCA
 2221 AGCTTCACAA GAGGTAAAAA ATTGGATGAC AGAAACCTTG TTGGTCCAAA ATGCGAACCC
 2281 AGATTGTAAG ACTATTTTAA AAGCATTGGG ACCAGGAGCG ACACTAGAAG AAATGATGAC
 2341 AGCATGTCAG GGAGTGGGGG GACCCGGCCA TAAAGCAAGA GTTTTGGCTG AAGCAATGAG
 2401 CCAAGTAACA AATCCAGCTA CCATAATGAT ACAGAAAGGC AATTTTAGGA ACCAAAGAAA
 2461 GACTGTTAAG TGTTTCAATT GTGGCAAAGA AGGGCACATA GCCAAAAATT GCAGGGCCCC
 2521 TAGGAAAAAG GGCTGTTGGA AATGTGGAAA GGAAGGACAC CAAATGAAAG ATTGTACTGA
 2581 GAGACAGGCT AATTTTTTAG GGAAGATCTG GCCTTCCCAC AAGGGAAGGC CAGGGAATTT
 2641 TCTTCAGAGC AGACCAGAGC CAACAGCCCC ACCAGAAGAG AGCTTCAGGT TTGGGGAAGA
 2701 GACAACAACT CCCTCTCAGA AGCAGGAGCC GATAGACAAG GAACTGTATC CTTTAGCTTC
 2761 CCTCAGATCA CTCTTTGGCA GCGACCCCTC GTCACAATAA AGATAGGGGG GCAATTAAAG
 2821 GAAGCTCTAT TAGATACAGG AGCAGATGAT ACAGTATTAG AAGAAATGAA TTTGCCAGGA
 2881 AGATGGAAAC CAAAAATGAT AGGGGGAATT GGAGGTTTTA TCAAAGTAAG ACAGTATGAT
 2941 CAGATACTCA TAGAAATCTG CGGACATAAA GCTATAGGTA CAGTATTAGT AGGACCTACA
 3001 CCTGTCAACA TAATTGGAAG AAATCTGTTG ACTCAGATTG GCTGCACTTT AAATTTTCCC
 3061 ATTAGTCCTA TTGAGACTGT ACCAGTAAAA TTAAAGCCAG GAATGGATGG CCCAAAAGTT
 3121 AAACAATGGC CATTGACAGA AGAAAAAATA AAAGCATTAG TAGAAATTTG TACAGAAATG
 3181 GAAAAGGAAG GAAAAATTTC AAAAATTGGG CCTGAAAATC CATACAATAC TCCAGTATTT
 3241 GCCATAAAGA AAAAAGACAG TACTAAATGG AGAAAATTAG TAGATTTCAG AGAACTTAAT
 3301 AAGAGAACTC AAGATTTCTG GGAAGTTCAA TTAGGAATAC CACATCCTGC AGGGTTAAAA
 3361 CAGAAAAAAT CAGTAACAGT ACTGGATGTG GGCGATGCAT ATTTTTCAGT TCCCTTAGAT
 3421 AAAGACTTCA GGAAGTATAC TGCATTTACC ATACCTAGTA TAAACAATGA GACACCAGGG
 3481 ATTAGATATC AGTACAATGT GCTTCCACAG GGATGGAAAG GATCACCAGC AATATTCCAG
 3541 TGTAGCATGA CAAAAATCTT AGAGCCTTTT AGAAAACAAA ATCCAGACAT AGTCATCTAT
 3601 CAATACATGG ATGATTTGTA TGTAGGATCT GACTTAGAAA TAGGGCAGCA TAGAACAAAA
 3661 ATAGAGGAAC TGAGACAACA TCTGTTGAGG TGGGGATTTA CCACACCAGA CAAAAAACAT
 3721 CAGAAAGAAC CTCCATTCCT TTGGATGGGT TATGAACTCC ATCCTGATAA ATGGACAGTA
 3781 CAGCCTATAG TGCTGCCAGA AAAGGACAGC TGGACTGTCA ATGACATACA GAAATTAGTG
 3841 GGAAAATTGA ATTGGGCAAG TCAGATTTAT GCAGGGATTA AAGTAAGGCA ATTATGTAAA
 3901 CTTCTTAGGG GAACCAAAGC ACTAACAGAA GTAGTACCAC TAACAGAAGA AGCAGAGCTA
 3961 GAACTGGCAG AAAACAGGGA GATTCTAAAA GAACCGGTAC ATGGAGTGTA TTATGACCCA
 4021 TCAAAAGACT TAATAGCAGA AATACAGAAG CAGGGGCAAG GCCAATGGAC ATATCAAATT
 4081 TATCAAGAGC CATTTAAAAA TCTGAAAACA GGAAAGTATG CAAGAATGAA GGGTGCCCAC
 4141 ACTAATGATG TGAAACAATT AACAGAGGCA GTACAAAAAA TAGCCACAGA AAGCATAGTA
 4201 ATATGGGGAA AGACTCCTAA ATTTAAATTA CCCATACAAA AGGAAACATG GGAAGCATGG
 4261 TGGACAGAGT ATTGGCAAGC CACCTGGATT CCTGAGTGGG AGTTTGTCAA TACCCCTCCC
 4321 TTAGTGAAGT TATGGTACCA GTTAGAGAAA GAACCCATAA TAGGAGCAGA AACTTTCTAT
 4381 GTAGATGGGG CAGCCAATAG GGAAACTAAA TTAGGAAAAG CAGGATATGT AACTGACAGA
 4441 GGAAGACAAA AAGTTGTCCC CCTAACGGAC ACAACAAATC AGAAGACTGA GTTACAAGCA
 4501 ATTCATCTAG CTTTGCAGGA TTCGGGATTA GAAGTAAACA TAGTGACAGA CTCACAATAT
 4561 GCATTGGGAA TCATTCAAGC ACAACCAGAT AAGAGTGAAT CAGAGTTAGT CAGTCAAATA
 4621 ATAGAGCAGT TAATAAAAAA GGAAAAAGTC TACCTGGCAT GGGTACCAGC ACACAAAGGA
 4681 ATTGGAGGAA ATGAACAAGT AGATAAATTG GTCAGTGCTG GAATCAGGAA AGTACTATTT
 4741 TTAGATGGAA TAGATAAGGC CCAAGAAGAA CATGAGAAAT ATCACAGTAA TTGGAGAGCA
 4801 ATGGCTAGTG ATTTTAACCT ACCACCTGTA GTAGCAAAAG AAATAGTAGC CAGCTGTGAT
 4861 AAATGTCAGC TAAAAGGGGA AGCCATGCAT GGACAAGTAG ACTGTAGCCC AGGAATATGG
 4921 CAGCTAGATT GTACACATTT AGAAGGAAAA GTTATCTTGG TAGCAGTTCA TGTAGCCAGT
 4981 GGATATATAG AAGCAGAAGT AATTCCAGCA GAGACAGGGC AAGAAACAGC ATACTTCCTC
 5041 TTAAAATTAG CAGGAAGATG GCCAGTAAAA ACAGTACATA CAGACAATGG CAGCAATTTC
 5101 ACCAGTACTA CAGTTAAGGC CGCCTGTTGG TGGGCGGGGA TCAAGCAGGA ATTTGGCATT
 5161 CCCTACAATC CCCAAAGTCA AGGAGTAATA GAATCTATGA ATAAAGAATT AAAGAAAATT
 5221 ATAGGACAGG TAAGAGATCA GGCTGAACAT CTTAAGACAG CAGTACAAAT GGCAGTATTC
 5281 ATCCACAATT TTAAAAGAAA AGGGGGGATT GGGGGGTACA GTGCAGGGGA AAGAATAGTA
 5341 GACATAATAG CAACAGACAT ACAAACTAAA GAATTACAAA AACAAATTAC AAAAATTCAA
 5401 AATTTTCGGG TTTATTACAG GGACAGCAGA GATCCAGTTT GGAAAGGACC AGCAAAGCTC
 5461 CTCTGGAAAG GTGAAGGGGC AGTAGTAATA CAAGATAATA GTGACATAAA AGTAGTGCCA
 5521 AGAAGAAAAG CAAAGATCAT CAGGGATTAT GGAAAACAGA TGGCAGGTGA TGATTGTGTG
 5581 GCAAGTAGAC AGGATGAGGA TTAACACATG GAATTCCGGA GCGGCCGCAG GAGCTTTGTT
 5641 CCTTGGGTTC TTGGGAGCAG CAGGAAGCAC TATGGGCGCA GCGTCAATGA CGCTGACGGT
 5701 ACAGGCCAGA CAATTATTGT CTGGTATAGT GCAGCAGCAG AACAATTTGC TGAGGGCTAT
 5761 TGAGGCGCAA CAGCATCTGT TGCAACTCAC AGTCTGGGGC ATCAAGCAGC TCCAGGCAAG
 5821 AATCCTGGCT GTGGAAAGAT ACCTAAAGGA TCAACAGCTC CTGGGGATTT GGGGTTGCTC
 5881 TGGAAAACTC ATTTGCACCA CTGCTGTGCC TTGGAATGCT AGTTGGAGTA ATAAATCTCT
 5941 GGAACAGATT TGGAATCACA CGACCTGGAT GGAGTGGGAC AGAGAAATTA ACAATTACAC
 6001 AAGCTTCCGC GGAATTCACC CCACCAGTGC AGGCTGCCTA TCAGAAAGTG GTGGCTGGTG
 6061 TGGCTAATGC CCTGGCCCAC AAGTATCACT AAGCTCGCTT TCTTGCTGTC CAATTTCTAT
 6121 TAAAGGTTCC TTTGTTCCCT AAGTCCAACT ACTAAACTGG GGGATATTAT GAAGGGCCTT
 6181 GAGCATCTGG ATTCTGCCTA ATAAAAAACA TTTATTTTCA TTGCAATGAT GTATTTAAAT
 6241 TATTTCTGAA TATTTTACTA AAAAGGGAAT GTGGGAGGTC AGTGCATTTA AAACATAAAG
 6301 AAATGAAGAG CTAGTTCAAA CCTTGGGAAA ATACACTATA TCTTAAACTC CATGAAAGAA
 6361 GGTGAGGCTG CAAACAGCTA ATGCACATTG GCAACAGCCC CTGATGCCTA TGCCTTATTC
 6421 ATCCCTCAGA AAAGGATTCA AGTAGAGGCT TGATTTGGAG GTTAAAGTTT TGCTATGCTG
 6481 TATTTTACAT TACTTATTGT TTTAGCTGTC CTCATGAATG TCTTTTCACT ACCCATTTGC
 6541 TTATCCTGCA TCTCTCAGCC TTGACTCCAC TCAGTTCTCT TGCTTAGAGA TACCACCTTT
 6601 CCCCTGAAGT GTTCCTTCCA TGTTTTACGG CGAGATGGTT TCTCCTCGCC TGGCCACTCA
 6661 GCCTTAGTTG TCTCTGTTGT CTTATAGAGG TCTACTTGAA GAAGGAAAAA CAGGGGGCAT
 6721 GGTTTGACTG TCCTGTGAGC CCTTCTTCCC TGCCTCCCCC ACTCACAGTG ACCCGGAATC
 6781 CCTCGACATG GCAGTCTAGC ACTAGTGCGG CCGCAGATCT GCTTCCTCGC TCACTGACTC
 6841 GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG
 6901 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA
 6961 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA
 7021 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG
 7081 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT
 7141 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG
 7201 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC
 7261 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT
 7321 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA
 7381 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC
 7441 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC
 7501 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT
 7561 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC
 7621 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT
 7681 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA
 7741 AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT
 7801 ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG
 7861 CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA
 7921 TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT
 7981 ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT
 8041 TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT
 8101 TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT
 8161 GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC
 8221 CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC
 8281 CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT
 8341 GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG
 8401 AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT
 8461 ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC
 8521 TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA
 8581 GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG
 8641 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA
 8701 TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TGGATCCCCT
 8761 GAGGGGGCCC CCATGGGCTA GAGGATCCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA
 8821 GTCAGCCATG AGCTTGGCCC ATTGCATACG TTGTATCCAT ATCATAATAT GTACATTTAT
 8881 ATTGGCTCAT
//

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