pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-P

LOCUS       pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro	10151 bp 	DNA	SYN
DEFINITION  pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..10151
                     /organism="pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro"
                     /mol_type="other DNA"
     promoter        10..242
                     /label="hU6_promoter"
     misc_feature    172..191
                     /label="LKO1_5_primer"
     misc_feature    394..663
                     /label="CAG_enhancer"
     CDS             complement(1180..3780)
                     /label="ORF frame 3"
     CDS             1201..5400
                     /label="ORF frame 1"
     misc_feature    1204..1230
                     /label="HA"
     misc_feature    1234..1254
                     /label="SV40 NLS"
     misc_feature    1240..1254
                     /label="NLS"
     misc_feature    complement(5425..5442)
                     /label="BGH_rev_primer"
     terminator      5428..5631
                     /label="bGH_PA_terminator"
     misc_feature    5787..5806
                     /label="H1_primer"
     misc_feature    6029..6050
                     /label="EGFP_C_primer"
     misc_feature    complement(6126..6159)
                     /label="loxP"
     misc_feature    complement(6255..6278)
                     /label="MSCV_rev_primer"
     CDS             6499..7356
                     /label="ORF frame 1"
     misc_feature    6650..6669
                     /label="mPGK_F_primer"
     CDS             complement(6730..7407)
                     /label="ORF frame 3"
     gene            6757..7356
                     /label="puro (variant)"
                     /gene="puro (variant)"
     rep_origin      7646..7952
                     /label="f1_origin"
     misc_feature    8220..8242
                     /label="pGEX_3_primer"
     promoter        8401..8429
                     /label="AmpR_promoter"
     gene            8471..9331
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             8471..9331
                     /label="ORF frame 2"
     rep_origin      9486..10105
                     /label="pBR322_origin"
ORIGIN
    1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
   61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
  121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
  181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt GTGGAAAGGA
  241 CGAAACACCG GGTTTTAGAG CTATGCTGTT TTGAATGGTC CCAAAACggG TCTTCgaGAA
  301 GACGTTTTAG AGCTATGCTG TTTTGAATGG TCCCAAAACT TTTTctagcg cgtgcgccaa
  361 ttctgcagac aaatggctct agaggtaccc gttacataac ttacggtaaa tggcccgcct
  421 ggctgaccgc ccaacgaccc ccgcccattg acgtcaatag taacgccaat agggactttc
  481 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
  541 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
  601 tgtgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
  661 atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc
  721 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg
  781 gcgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg
  841 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
  901 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
  961 gctgcgacgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg
 1021 gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg
 1081 ctgtaattag ctgagcaaga ggtaagggtt taagggatgg ttggttggtg gggtattaat
 1141 gtttaattac ctggagcacc tgcctgaaat cacttttttt caggttGGac cggtgccacc
 1201 ATGTACCCAT ACGATGTTCC AGATTACGCT TCGCCGAAGA AAAAGCGCAA GGTCGAAGCG
 1261 TCCGACAAGA AGTACAGCAT CGGCCTGGCC ATCGGCACCA ACTCTGTGGG CTGGGCCGTG
 1321 ATCACCGACG AGTACAAGGT GCCCAGCAAG AAATTCAAGG TGCTGGGCAA CACCGACCGG
 1381 CACAGCATCA AGAAGAACCT GATCGGAGCC CTGCTGTTCG ACAGCGGCGA AACAGCCGAG
 1441 GCCACCCGGC TGAAGAGAAC CGCCAGAAGA AGATACACCA GACGGAAGAA CCGGATCTGC
 1501 TATCTGCAAG AGATCTTCAG CAACGAGATG GCCAAGGTGG ACGACAGCTT CTTCCACAGA
 1561 CTGGAAGAGT CCTTCCTGGT GGAAGAGGAT AAGAAGCACG AGCGGCACCC CATCTTCGGC
 1621 AACATCGTGG ACGAGGTGGC CTACCACGAG AAGTACCCCA CCATCTACCA CCTGAGAAAG
 1681 AAACTGGTGG ACAGCACCGA CAAGGCCGAC CTGCGGCTGA TCTATCTGGC CCTGGCCCAC
 1741 ATGATCAAGT TCCGGGGCCA CTTCCTGATC GAGGGCGACC TGAACCCCGA CAACAGCGAC
 1801 GTGGACAAGC TGTTCATCCA GCTGGTGCAG ACCTACAACC AGCTGTTCGA GGAAAACCCC
 1861 ATCAACGCCA GCGGCGTGGA CGCCAAGGCC ATCCTGTCTG CCAGACTGAG CAAGAGCAGA
 1921 CGGCTGGAAA ATCTGATCGC CCAGCTGCCC GGCGAGAAGA AGAATGGCCT GTTCGGCAAC
 1981 CTGATTGCCC TGAGCCTGGG CCTGACCCCC AACTTCAAGA GCAACTTCGA CCTGGCCGAG
 2041 GATGCCAAAC TGCAGCTGAG CAAGGACACC TACGACGACG ACCTGGACAA CCTGCTGGCC
 2101 CAGATCGGCG ACCAGTACGC CGACCTGTTT CTGGCCGCCA AGAACCTGTC CGACGCCATC
 2161 CTGCTGAGCG ACATCCTGAG AGTGAACACC GAGATCACCA AGGCCCCCCT GAGCGCCTCT
 2221 ATGATCAAGA GATACGACGA GCACCACCAG GACCTGACCC TGCTGAAAGC TCTCGTGCGG
 2281 CAGCAGCTGC CTGAGAAGTA CAAAGAGATT TTCTTCGACC AGAGCAAGAA CGGCTACGCC
 2341 GGCTACATTG ACGGCGGAGC CAGCCAGGAA GAGTTCTACA AGTTCATCAA GCCCATCCTG
 2401 GAAAAGATGG ACGGCACCGA GGAACTGCTC GTGAAGCTGA ACAGAGAGGA CCTGCTGCGG
 2461 AAGCAGCGGA CCTTCGACAA CGGCAGCATC CCCCACCAGA TCCACCTGGG AGAGCTGCAC
 2521 GCCATTCTGC GGCGGCAGGA AGATTTTTAC CCATTCCTGA AGGACAACCG GGAAAAGATC
 2581 GAGAAGATCC TGACCTTCCG CATCCCCTAC TACGTGGGCC CTCTGGCCAG GGGAAACAGC
 2641 AGATTCGCCT GGATGACCAG AAAGAGCGAG GAAACCATCA CCCCCTGGAA CTTCGAGGAA
 2701 GTGGTGGACA AGGGCGCTTC CGCCCAGAGC TTCATCGAGC GGATGACCAA CTTCGATAAG
 2761 AACCTGCCCA ACGAGAAGGT GCTGCCCAAG CACAGCCTGC TGTACGAGTA CTTCACCGTG
 2821 TATAACGAGC TGACCAAAGT GAAATACGTG ACCGAGGGAA TGAGAAAGCC CGCCTTCCTG
 2881 AGCGGCGAGC AGAAAAAGGC CATCGTGGAC CTGCTGTTCA AGACCAACCG GAAAGTGACC
 2941 GTGAAGCAGC TGAAAGAGGA CTACTTCAAG AAAATCGAGT GCTTCGACTC CGTGGAAATC
 3001 TCCGGCGTGG AAGATCGGTT CAACGCCTCC CTGGGCACAT ACCACGATCT GCTGAAAATT
 3061 ATCAAGGACA AGGACTTCCT GGACAATGAG GAAAACGAGG ACATTCTGGA AGATATCGTG
 3121 CTGACCCTGA CACTGTTTGA GGACAGAGAG ATGATCGAGG AACGGCTGAA AACCTATGCC
 3181 CACCTGTTCG ACGACAAAGT GATGAAGCAG CTGAAGCGGC GGAGATACAC CGGCTGGGGC
 3241 AGGCTGAGCC GGAAGCTGAT CAACGGCATC CGGGACAAGC AGTCCGGCAA GACAATCCTG
 3301 GATTTCCTGA AGTCCGACGG CTTCGCCAAC AGAAACTTCA TGCAGCTGAT CCACGACGAC
 3361 AGCCTGACCT TTAAAGAGGA CATCCAGAAA GCCCAGGTGT CCGGCCAGGG CGATAGCCTG
 3421 CACGAGCACA TTGCCAATCT GGCCGGCAGC CCCGCCATTA AGAAGGGCAT CCTGCAGACA
 3481 GTGAAGGTGG TGGACGAGCT CGTGAAAGTG ATGGGCCGGC ACAAGCCCGA GAACATCGTG
 3541 ATCGAAATGG CCAGAGAGAA CCAGACCACC CAGAAGGGAC AGAAGAACAG CCGCGAGAGA
 3601 ATGAAGCGGA TCGAAGAGGG CATCAAAGAG CTGGGCAGCC AGATCCTGAA AGAACACCCC
 3661 GTGGAAAACA CCCAGCTGCA GAACGAGAAG CTGTACCTGT ACTACCTGCA GAATGGGCGG
 3721 GATATGTACG TGGACCAGGA ACTGGACATC AACCGGCTGT CCGACTACGA TGTGGACCAT
 3781 ATCGTGCCTC AGAGCTTTCT GAAGGACGAC TCCATCGACA ACAAGGTGCT GACCAGAAGC
 3841 GACAAGAACC GGGGCAAGAG CGACAACGTG CCCTCCGAAG AGGTCGTGAA GAAGATGAAG
 3901 AACTACTGGC GGCAGCTGCT GAACGCCAAG CTGATTACCC AGAGAAAGTT CGACAATCTG
 3961 ACCAAGGCCG AGAGAGGCGG CCTGAGCGAA CTGGATAAGG CCGGCTTCAT CAAGAGACAG
 4021 CTGGTGGAAA CCCGGCAGAT CACAAAGCAC GTGGCACAGA TCCTGGACTC CCGGATGAAC
 4081 ACTAAGTACG ACGAGAATGA CAAGCTGATC CGGGAAGTGA AAGTGATCAC CCTGAAGTCC
 4141 AAGCTGGTGT CCGATTTCCG GAAGGATTTC CAGTTTTACA AAGTGCGCGA GATCAACAAC
 4201 TACCACCACG CCCACGACGC CTACCTGAAC GCCGTCGTGG GAACCGCCCT GATCAAAAAG
 4261 TACCCTAAGC TGGAAAGCGA GTTCGTGTAC GGCGACTACA AGGTGTACGA CGTGCGGAAG
 4321 ATGATCGCCA AGAGCGAGCA GGAAATCGGC AAGGCTACCG CCAAGTACTT CTTCTACAGC
 4381 AACATCATGA ACTTTTTCAA GACCGAGATT ACCCTGGCCA ACGGCGAGAT CCGGAAGCGG
 4441 CCTCTGATCG AGACAAACGG CGAAACCGGG GAGATCGTGT GGGATAAGGG CCGGGATTTT
 4501 GCCACCGTGC GGAAAGTGCT GAGCATGCCC CAAGTGAATA TCGTGAAAAA GACCGAGGTG
 4561 CAGACAGGCG GCTTCAGCAA AGAGTCTATC CTGCCCAAGA GGAACAGCGA TAAGCTGATC
 4621 GCCAGAAAGA AGGACTGGGA CCCTAAGAAG TACGGCGGCT TCGACAGCCC CACCGTGGCC
 4681 TATTCTGTGC TGGTGGTGGC CAAAGTGGAA AAGGGCAAGT CCAAGAAACT GAAGAGTGTG
 4741 AAAGAGCTGC TGGGGATCAC CATCATGGAA AGAAGCAGCT TCGAGAAGAA TCCCATCGAC
 4801 TTTCTGGAAG CCAAGGGCTA CAAAGAAGTG AAAAAGGACC TGATCATCAA GCTGCCTAAG
 4861 TACTCCCTGT TCGAGCTGGA AAACGGCCGG AAGAGAATGC TGGCCTCTGC CGGCGAACTG
 4921 CAGAAGGGAA ACGAACTGGC CCTGCCCTCC AAATATGTGA ACTTCCTGTA CCTGGCCAGC
 4981 CACTATGAGA AGCTGAAGGG CTCCCCCGAG GATAATGAGC AGAAACAGCT GTTTGTGGAA
 5041 CAGCACAAGC ACTACCTGGA CGAGATCATC GAGCAGATCA GCGAGTTCTC CAAGAGAGTG
 5101 ATCCTGGCCG ACGCTAATCT GGACAAAGTG CTGTCCGCCT ACAACAAGCA CCGGGATAAG
 5161 CCCATCAGAG AGCAGGCCGA GAATATCATC CACCTGTTTA CCCTGACCAA TCTGGGAGCC
 5221 CCTGCCGCCT TCAAGTACTT TGACACCACC ATCGACCGGA AGAGGTACAC CAGCACCAAA
 5281 GAGGTGCTGG ACGCCACCCT GATCCACCAG AGCATCACCG GCCTGTACGA GACACGGATC
 5341 GACCTGTCTC AGCTGGGAGG CGACAGCCCC AAGAAGAAGA GAAAGGTGGA GGCCAGCtaa
 5401 gaattcCTAG AGCTCGCTGA TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG
 5461 TTTGCCCCTC CCCCGTGCCT TCCTTGACCC TGGAAGGTGC CACTCCCACT GTCCTTTCCT
 5521 AATAAAATGA GGAAATTGCA TCGCATTGTC TGAGTAGGTG TCATTCTATT CTGGGGGGTG
 5581 GGGTGGGGCA GGACAGCAAG GGGGAGGATT GGGAAGAgAA TAGCAGGCAT GCTGGGGAgc
 5641 ggccgcAATT CGAACGCTGA CGTCATCAAC CCGCTCCAAG GAATCGCGGG CCCAGTGTCA
 5701 CTAGGCGGGA ACACCCAGCG CGCGTGCGCC CTGGCAGGAA GATGGCTGTG AGGGACAGGG
 5761 GAGTGGCGCC CTGCAATATT TGCATGTCGC TATGTGTTCT GGGAAATCAC CATAAACGTG
 5821 AAATGTCTTT GGATTTGGGA ATCTTATAAG TTCTGTATGA GACCACAGAT CTAgttggaa
 5881 ccattcaaaa cagcatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
 5941 gcaccgagtc ggtgcttttt ggcgccgaca accactacct gagcacccag tccgccctga
 6001 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
 6061 ggatcactct cggcatggac gagctgtaca agtaaGAATT Ccgatcatat tcaataaccc
 6121 ttaatataac ttcgtataat gtatgctata cgaagttatt aggtctgaag aggagtttac
 6181 gtccagccaa gcttaggatc tcgacctcga aattctaccg ggtaggggag gcgcttttcc
 6241 caaggcagtc tggagcatgc gctttagcag ccccgctggc acttggcgct acacaagtgg
 6301 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg
 6361 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca
 6421 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat
 6481 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct
 6541 ttgctccttc gctttctggg ctcagcagct gggaagggtg ggtccggggg cgggctcagg
 6601 ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg
 6661 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc
 6721 tgcatccatc tagatctcga gcagctgaag cttaccatga ccgagtacaa gcccacggtg
 6781 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
 6841 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
 6901 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
 6961 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
 7021 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
 7081 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
 7141 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
 7201 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
 7261 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
 7321 cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc
 7381 ccgaccgaaa ggagcgcacg accccatgca tcgatgatat cagatccccg ggatgcagaa
 7441 attgatgatc tattaaacaa taaagatgtc cactaaaatg gaagtttttc ctgtcatact
 7501 ttgttaagaa gggtgagaac agagtaccta cattttgaat ggaaggattg gagcggcgcc
 7561 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca
 7621 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
 7681 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
 7741 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
 7801 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg
 7861 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
 7921 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt
 7981 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
 8041 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag
 8101 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
 8161 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
 8221 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
 8281 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
 8341 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
 8401 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
 8461 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
 8521 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
 8581 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
 8641 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
 8701 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
 8761 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
 8821 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
 8881 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
 8941 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
 9001 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
 9061 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
 9121 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
 9181 gcgtggaagc cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
 9241 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
 9301 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
 9361 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
 9421 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
 9481 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
 9541 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
 9601 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
 9661 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
 9721 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
 9781 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
 9841 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
 9901 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
 9961 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
10021 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
10081 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
10141 tgctcacatg t
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