质粒名称: | pMXs-hSOX2 |
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出品公司: | 爱迪基因 |
目录编号: | 17218 |
存储实验室: | Shinya Yamanaka |
质粒骨架基本信息 |
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质粒名称: | pMXs |
质粒抗性: | 氨苄霉素 |
空质粒大小: | 4600 bp |
质粒类型: | 哺乳细胞表达 |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | SOX2 |
物种来源: | H. sapiens (human) |
插入大小: | 960 bp |
基因突变: | None |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | attB1(未破坏) |
3' 克隆位点: | attB2(未破坏) |
5' 测序引物 | pMX-S1811 |
3' 测序引物 | |
文献引用方法 |
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材料和方法部分: | pMXs-hSOX2 was a gift from Shinya Yamanaka (Addgene plasmid # 17218) |
参考文献部分: | Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S. Cell. 2007 Nov 30. 131(5):861-72. 10.1016/j.cell.2007.11.019 PubMed 18035408 |
pMXs-hSOX2
价格:1200元
联系方式:I47-825O-882O
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质粒基本信息
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质粒简介
质粒序列
LOCUS pMXs-hSOX2 Sequencing Result 5721 bp DNA SYN DEFINITION pMXs-hSOX2 Sequencing Result ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5721 /organism="pMXs-hSOX2 Sequencing Result" /mol_type="other DNA" misc_feature complement(130..636) /label="3MoMuLV_LTR" misc_feature complement(130..594) /label="5_LTR2" misc_feature complement(831..855) /label="attB2" CDS complement(872..1825) /label="ORF frame 3" misc_feature complement(1846..1870) /label="attB1" misc_feature complement(1919..3200) /label="psi_plus_pack2" misc_feature complement(1967..1983) /label="pBMN_5_primer" misc_feature complement(2236..2715) /label="gag" misc_feature complement(2321..2337) /label="pBABE_5_primer" misc_feature complement(2353..2375) /label="pLXSN_5_primer" misc_feature complement(2353..2375) /label="MSCV_primer" misc_feature complement(3200..3751) /label="5_LTR2" misc_feature complement(3200..3793) /label="3MoMuLV_LTR" promoter 3858..3886 /label="AmpR_promoter" gene 3928..4788 /label="Ampicillin" /gene="Ampicillin" CDS 3928..4788 /label="ORF frame 1" rep_origin 4936..5555 /label="pBR322_origin" ORIGIN 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgCAACTATG 61 GCCATTTAAT GTAAATACTT AAGAAAAAAA ACCAAATTAA TTTTGATACA TGCTGCATGT 121 GAAAGACCCC CGCTGACGGG TAGTCAATCA CTCAGAGGAG ACCCTCCCAA GGAACAGCGA 181 GACCACAAGT CGGATGCAAC TGCAAGAGGG TTTATTGGAT ACACGGGTAC CCGGGCGACT 241 CAGTCAATCG GAGGACTGGC GCGCCGAGTG AGGGGTTGTG GGCTCTTTTA TTGAGCTCGG 301 GGAGCAGAAG CGCGCGAACA GAAGCGAGAA GCGAACTGAT TGGTTAGTTC AAATAAGGCA 361 CAGGGTCATT TCAGGTCCTT GGGGCACCCT GGAAACATCT GATGGTTCTC TAGAAACTGC 421 TGAGGGCTGG ACCGCATCTG GGGACCATCT GTTCTTGGCC CTGAGCCGGG GCAGGAACTG 481 CTTACCACAG ATATCCTGTT TGGCCCATAT TCAGCTGTTC CATCTGTTCC TGACCTTGAT 541 CTGAACTTTT CTATTCTCAG TTATGTATTT TTCCATGCCT TGCAAAATGG CGTTACTTAA 601 GCTAGCTTGC CAAACCTACA GGTGGGGTCT TTCATTCCCC CCTTTTTCTG GAGACTAAAT 661 AAAATCTTTT ATTTTATCGT CGACCACTGT GCTGGCGGCC GCTCGAGTTT AAATAGTACA 721 TAATGGATTT CCTTACGCGA AATACGGGCA GACATGGCCT GCCCGGTTAT TATTATTTTT 781 GACACCAGAC CAACTGGTAA TGGTAGCGAC CGGCGCTCAG CTGGAATATC ACCACTTTGT 841 ACAAGAAAGC TGGGTCGGCG CGCCCACCCT TTCACATGTG TGAGAGGGGC AGTGTGCCGT 901 TAATGGCCGT GCCGGGCACC GGGCCGCTCT GGTAGTGCTG GGACATGTGA AGTCTGCTGG 961 GGGCGGCGGG TTCCGGCACC TCGGCGCCGG GGAGATACAT GCTGATCATG TCCCGGAGGT 1021 CCCCGGCCTG GCAGGGCGCC CTGGAGTGGG AGGAAGAGGT AACCACAGGG GGGCTGGAGC 1081 TGGCCTCGGA CTTGACCACC GAACCCATGG AGCCAAGAGC CATGCCAGGG GTGCCCTGCT 1141 GCGAGTAGGA CATGCTGTAG GTGGGCGAGC CGTTCATGTA GGTCTGCGAG CTGGTCATGG 1201 AGTTGTACTG CAGGGCGCTC ACGTCGTAGC GGTGCATGGG CTGCATCTGC GCTGCGCCGT 1261 GCGCATTGAG GCCCGGGTGC TGCGGGTAGC CCAGCTGGTC CTGCATCATG CTGTAGCTGC 1321 CGTTGCTCCA GCCGTTCATG TGCGCGTAAC TGTCCATGCG CTGGTTCACG CCCGCGCCCA 1381 GGCCGGCGCC CACCCCGACC CCGCTCGCCA TGCTATTGCC GCCGGGGGCC AGCAGCCCGC 1441 CGGGCAGCGT GTACTTATCC TTCTTCATGA GCGTCTTGGT TTTCCGCCGG GGCCGGTATT 1501 TATAATCCGG GTGCTCCTTC ATGTGCAGCG CTCGCAGCCG CTTAGCCTCG TCGATGAACG 1561 GCCGCTTCTC CGTCTCCGAC AAAAGTTTCC ACTCGGCGCC CAGGCGCTTG CTGATCTCCG 1621 AGTTGTGCAT CTTGGGGTTC TCCTGGGCCA TCTTGCGCCG CTGCCCGCGG GACCACACCA 1681 TGAAGGCATT CATGGGCCGC TTGACGCGGT CCGGGCTGTT TTTCTGGTTG CCGCCGGCCG 1741 CCGCCGCGGT GGAGTTGCCG CCGCCGCCCC CCGAAGTTTG CTGCGGGCCC GGCGGCTTCA 1801 GCTCCGTCTC CATCATGTTG TACATGGTGA AGGGGGCGGC CGCGGAGCCT GCTTTTTTGT 1861 ACAAACTTGT GATTTCCCGT ACCACCACAC TGGGATCCTT AATTAACTAG CTAGATCCGG 1921 CAGTCTAGAG GATGGTCCAC CCCCGGGGTC GGCAGCCTTC ACGTGGGCGG CGTGTATCCA 1981 AGCTGCGATG CCGTCTACTT TGAGGGCGGT GGGGGTGGTC AGCAGGACTG TGTAAGGTCC 2041 TTTCCAGCGA GGTTCTAGGT TCTTAGTCTG GTGTCGGCGG ACCCACACTG TGTCGCCGAC 2101 TCGGTAAGGG TGAGGTACCA CCGGTCGGTC CAGTTGTTCT TGGTAGGCTG CCGCCAGAGG 2161 TCTCCAGACT TCGTGCTGGA CTAAGTAGAG AGCCTGTAAG TGAGCTTGGA GAGAGGGGCT 2221 GTTAGTAACT CTTGTCATGT CAGGGTCAGG GAAGTTTACA AGGGGCGGGG GTGCCCCATA 2281 TAAGATCTCA TATGGCCATA TGGGGGCGCC TAGAGAAGGA GTGAGGGCTG GATAAAGGGA 2341 GGATCGAGGC GGGGTCGAAC GAGGAGGTTC AAGGGGGAGA GACGGGGCGG ATGGAGGAAG 2401 AGGAGGCGGA GGCTTAGGGT GTACAAAGGG CTTGACCCAG GGAGGGGGGT CAAAAGCCAA 2461 GGCTTCCCAG GTCACGATGT AGGGGACCTG GTCTGGGTGT CCATGCGGGC CAGGTGAAAA 2521 GACCTTGATC TTAACCTGGG TGATGAGGTC TCGGTTAAAG GTGCCGTCTC GCGGCCATCC 2581 GACGTTAAAG GTTGGCCATT CTGCAGAGCA GAAGGTAACC CAACGTCTCT TCTTGACATC 2641 TACCGACTGG TTGTGAGCGA TCCGCTCGAC ATCTTTCCAG TGACCTAAGG TCAAACTTAA 2701 GGGAGTGGTA ACAGTCTGGC CCTAATTTTC AGACAAATAC AGAAACACAG TCAGACAGAG 2761 ACAACACAGA ACGATGCTGC AGCAGACAAG ACGCGCGGCG CGGCTTCGGT CCCAAACCGA 2821 AAGCAAAAAT TCAGACGGAG GCGGGAACTG TTTTAGGTTC TCGTCTCCTA CCAGAACCAC 2881 ATATCCCTCC TATTAGGGGG GTGCACCAAA GAGTCCAAAA CGATCGGGAT TTTTGGACTC 2941 AGGTCGGGCC ACAAAAACGG CCCCCGAAGT CCCTGGGACG TCTCCCAGGG TTGCGGCCGG 3001 GTGTTCCGAA CTCGTCAGTT CCACCACGGG TCCGCCAGAT ACAGAGCTAG TTAGCTAACT 3061 AGTACCGACG CAGGCGCATA AAATCAGTCA TAGACACTAG ACAATCGGAC AGACACAGAT 3121 AAGTTGCTGG CCAGCTTACC TCCCGGTGGT GGGTCGGTGG TCCCTGGGCA GGGGTCTCCC 3181 GATCCCGGAC GAGCCCCCAA ATGAAAGACC CCCGCTGACG GGTAGTCAAT CACTCAGAGG 3241 AGACCCTCCC AAGGAACAGC GAGACCACAA GTCGGATGCA ACTGCAAGAG GGTTTATTGG 3301 ATACACGGGT ACCCGGGCGA CTCAGTCAAT CGGAGGACTG GCGCGCCGAG TGAGGGGTTG 3361 TGGGCTCTTT TATTGAGCTC GGGGAGCAGA AGCGCGCGAA CAGAAGCGAG AAGCGAACTG 3421 ATTGGTTAGT TCAAATAAGG CACAGGGTCA TTTCAGGTCC TTGGGGCACC CTGGAAACAT 3481 CTGATGGTTC TCTAGAAACT GCTGAGGGCT GGACCGCATC TGGGGACCAT CTGTTCTTGG 3541 CCCTGAGCCG GGGCAGGAAC TGCTTACCAC AGATATCCTG TTTGGCCCAT ATTCAGCTGT 3601 TCCATCTGTT CTTGGCCCTG AGCCGGGGCA GGAACTGCTT ACCACAGATA TCCTGTTTGG 3661 CCCATATTCA GCTGTTCCAT CTGTTCCTGA CCTTGATCTG AACTTTTCTA TTCTCAGTTA 3721 TGTATTTTTC CATGCCTTGC AAAATGGCGT TACTTAAGCT AGCTTGCCAA ACCTACAGGT 3781 GGGGTCTTTC ATTATGCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT 3841 TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG 3901 CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT 3961 CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA 4021 AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC 4081 GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA 4141 GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC 4201 CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT 4261 ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT 4321 GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC 4381 AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA 4441 CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA 4501 TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG 4561 GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT 4621 AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT 4681 AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA 4741 AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA 4801 GTTTACTCAT ATATACTTTA GATTGATTTG CGGCCGGCCG CAAAAGGATC TAGGTGAAGA 4861 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 4921 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 4981 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 5041 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC 5101 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 5161 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 5221 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 5281 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 5341 AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 5401 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 5461 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 5521 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 5581 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA 5641 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT 5701 CAGTGAGCGA GGAAGCGGAA G //
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