pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA

LOCUS       pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA	7293 bp 	DNA	SYN
DEFINITION  pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..7293
                     /organism="pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA"
                     /mol_type="other DNA"
     CDS             complement(591..3338)
                     /label="ORF frame 2"
     CDS             621..3953
                     /label="ORF frame 3"
     misc_feature    627..647
                     /label="SV40 NLS"
     misc_feature    627..650
                     /label="SV40 NLS"
     misc_feature    633..647
                     /label="NLS"
     CDS             complement(1832..2290)
                     /label="ORF frame 3"
     misc_feature    3882..3908
                     /label="HA"
     misc_feature    complement(3978..3995)
                     /label="BGH_rev_primer"
     terminator      3981..4184
                     /label="bGH_PA_terminator"
     promoter        complement(4306..4545)
                     /label="hU6_promoter"
     misc_feature    complement(4357..4376)
                     /label="LKO1_5_primer"
     rep_origin      4788..5094
                     /label="f1_origin"
     misc_feature    5362..5384
                     /label="pGEX_3_primer"
     promoter        5543..5571
                     /label="AmpR_promoter"
     gene            5613..6473
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             5613..6473
                     /label="ORF frame 3"
     rep_origin      6628..7247
                     /label="pBR322_origin"
ORIGIN
    1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
   61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
  121 aggggttcct gcggcctcta gactcgaggg gctggaagct acctttgaca tcatttcctc
  181 tgcgaatgca tgtataattt ctacagaacc tattagaaag gatcacccag cctctgcttt
  241 tgtacaactt tcccttaaaa aactgccaat tccactgctg tttggcccaa tagtgagaac
  301 tttttcctgc tgcctcttgg tgcttttgcc tatggcccct attctgcctg ctgaagacac
  361 tcttgccagc atggacttaa acccctccag ctctgacaat cctctttctc ttttgtttta
  421 catgaagggt ctggcagcca aagcaatcac tcaaagttca aaccttatca ttttttgctt
  481 tgttcctctt ggccttggtt ttgtacatca gctttgaaaa taccatccca gggttaatgc
  541 tggggttaat ttataactaa gagtgctcta gttttgcaat acaggacatg ctataaaaat
  601 ggaaagatac cggtgccacc atggccccaa agaagaagcg gaaggtcggt atccacggag
  661 tcccagcagc caagcggaac tacatcctgg gcctggacat cggcatcacc agcgtgggct
  721 acggcatcat cgactacgag acacgggacg tgatcgatgc cggcgtgcgg ctgttcaaag
  781 aggccaacgt ggaaaacaac gagggcaggc ggagcaagag aggcgccaga aggctgaagc
  841 ggcggaggcg gcatagaatc cagagagtga agaagctgct gttcgactac aacctgctga
  901 ccgaccacag cgagctgagc ggcatcaacc cctacgaggc cagagtgaag ggcctgagcc
  961 agaagctgag cgaggaagag ttctctgccg ccctgctgca cctggccaag agaagaggcg
 1021 tgcacaacgt gaacgaggtg gaagaggaca ccggcaacga gctgtccacc agagagcaga
 1081 tcagccggaa cagcaaggcc ctggaagaga aatacgtggc cgaactgcag ctggaacggc
 1141 tgaagaaaga cggcgaagtg cggggcagca tcaacagatt caagaccagc gactacgtga
 1201 aagaagccaa acagctgctg aaggtgcaga aggcctacca ccagctggac cagagcttca
 1261 tcgacaccta catcgacctg ctggaaaccc ggcggaccta ctatgaggga cctggcgagg
 1321 gcagcccctt cggctggaag gacatcaaag aatggtacga gatgctgatg ggccactgca
 1381 cctacttccc cgaggaactg cggagcgtga agtacgccta caacgccgac ctgtacaacg
 1441 ccctgaacga cctgaacaat ctcgtgatca ccagggacga gaacgagaag ctggaatatt
 1501 acgagaagtt ccagatcatc gagaacgtgt tcaagcagaa gaagaagccc accctgaagc
 1561 agatcgccaa agaaatcctc gtgaacgaag aggatattaa gggctacaga gtgaccagca
 1621 ccggcaagcc cgagttcacc aacctgaagg tgtaccacga catcaaggac attaccgccc
 1681 ggaaagagat tattgagaac gccgagctgc tggatcagat tgccaagatc ctgaccatct
 1741 accagagcag cgaggacatc caggaagaac tgaccaatct gaactccgag ctgacccagg
 1801 aagagatcga gcagatctct aatctgaagg gctataccgg cacccacaac ctgagcctga
 1861 aggccatcaa cctgatcctg gacgagctgt ggcacaccaa cgacaaccag atcgctatct
 1921 tcaaccggct gaagctggtg cccaagaagg tggacctgtc ccagcagaaa gagatcccca
 1981 ccaccctggt ggacgacttc atcctgagcc ccgtcgtgaa gagaagcttc atccagagca
 2041 tcaaagtgat caacgccatc atcaagaagt acggcctgcc caacgacatc attatcgagc
 2101 tggcccgcga gaagaactcc aaggacgccc agaaaatgat caacgagatg cagaagcgga
 2161 accggcagac caacgagcgg atcgaggaaa tcatccggac caccggcaaa gagaacgcca
 2221 agtacctgat cgagaagatc aagctgcacg acatgcagga aggcaagtgc ctgtacagcc
 2281 tggaagccat ccctctggaa gatctgctga acaacccctt caactatgag gtggaccaca
 2341 tcatccccag aagcgtgtcc ttcgacaaca gcttcaacaa caaggtgctc gtgaagcagg
 2401 aagaaaacag caagaagggc aaccggaccc cattccagta cctgagcagc agcgacagca
 2461 agatcagcta cgaaaccttc aagaagcaca tcctgaatct ggccaagggc aagggcagaa
 2521 tcagcaagac caagaaagag tatctgctgg aagaacggga catcaacagg ttctccgtgc
 2581 agaaagactt catcaaccgg aacctggtgg ataccagata cgccaccaga ggcctgatga
 2641 acctgctgcg gagctacttc agagtgaaca acctggacgt gaaagtgaag tccatcaatg
 2701 gcggcttcac cagctttctg cggcggaagt ggaagtttaa gaaagagcgg aacaaggggt
 2761 acaagcacca cgccgaggac gccctgatca ttgccaacgc cgatttcatc ttcaaagagt
 2821 ggaagaaact ggacaaggcc aaaaaagtga tggaaaacca gatgttcgag gaaaagcagg
 2881 ccgagagcat gcccgagatc gaaaccgagc aggagtacaa agagatcttc atcacccccc
 2941 accagatcaa gcacattaag gacttcaagg actacaagta cagccaccgg gtggacaaga
 3001 agcctaatag agagctgatt aacgacaccc tgtactccac ccggaaggac gacaagggca
 3061 acaccctgat cgtgaacaat ctgaacggcc tgtacgacaa ggacaatgac aagctgaaaa
 3121 agctgatcaa caagagcccc gaaaagctgc tgatgtacca ccacgacccc cagacctacc
 3181 agaaactgaa gctgattatg gaacagtacg gcgacgagaa gaatcccctg tacaagtact
 3241 acgaggaaac cgggaactac ctgaccaagt actccaaaaa ggacaacggc cccgtgatca
 3301 agaagattaa gtattacggc aacaaactga acgcccatct ggacatcacc gacgactacc
 3361 ccaacagcag aaacaaggtc gtgaagctgt ccctgaagcc ctacagattc gacgtgtacc
 3421 tggacaatgg cgtgtacaag ttcgtgaccg tgaagaatct ggatgtgatc aaaaaagaaa
 3481 actactacga agtgaatagc aagtgctatg aggaagctaa gaagctgaag aagatcagca
 3541 accaggccga gtttatcgcc tccttctaca acaacgatct gatcaagatc aacggcgagc
 3601 tgtatagagt gatcggcgtg aacaacgacc tgctgaaccg gatcgaagtg aacatgatcg
 3661 acatcaccta ccgcgagtac ctggaaaaca tgaacgacaa gaggcccccc aggatcatta
 3721 agacaatcgc ctccaagacc cagagcatta agaagtacag cacagacatt ctgggcaacc
 3781 tgtatgaagt gaaatctaag aagcaccctc agatcatcaa aaagggcaaa aggccggcgg
 3841 ccacgaaaaa ggccggccag gcaaaaaaga aaaagggatc ctacccatac gatgttccag
 3901 attacgctag cggcttcgcc aacgagcttg gacccaggtt gatgggaaag taagaattcc
 3961 tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
 4021 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
 4081 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
 4141 gcaggacagc aagggggagg attgggaaga gaatagcagg catgctgggg aggtaccaaa
 4201 aatctcgcca acaagttgac gagataaaca cggcattttg ccttgtttta gtagattctg
 4261 tttccagagt actaaaactg agacctgccg tggtctccgg tgtttcgtcc tttccacaag
 4321 atatataaag ccaagaaatc gaaatacttt caagttacgg taagcatatg atagtccatt
 4381 ttaaaacata attttaaaac tgcaaactac ccaagaaatt attactttct acgtcacgta
 4441 ttttgtacta atatctttgt gtttacagtc aaattaattc caattatctc tctaacagcc
 4501 ttgtatcgta tatgcaaata tgaaggaatc atgggaaata ggccctcgcg gccgcaggaa
 4561 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
 4621 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
 4681 cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
 4741 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
 4801 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
 4861 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
 4921 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
 4981 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
 5041 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
 5101 accctatctc gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt
 5161 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
 5221 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
 5281 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
 5341 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
 5401 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
 5461 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
 5521 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
 5581 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
 5641 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
 5701 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
 5761 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
 5821 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
 5881 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
 5941 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
 6001 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
 6061 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
 6121 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
 6181 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
 6241 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
 6301 ctgataaatc tggagccggt gagcgtggaa gccgcggtat cattgcagca ctggggccag
 6361 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
 6421 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
 6481 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
 6541 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
 6601 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
 6661 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
 6721 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
 6781 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
 6841 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
 6901 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
 6961 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
 7021 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
 7081 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
 7141 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
 7201 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
 7261 tcctggcctt ttgctggcct tttgctcaca tgt
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