pMSCV-Peredox-mCherry-NLS

价格:1200元

联系方式:I47-825O-882O

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质粒基本信息

质粒名称: pMSCV-Peredox-mCherry-NLS 
出品公司: 爱迪基因
目录编号: 32385
存储实验室: Gary Yellen

载体骨架基本信息
载体名称: pMSCV
载体出品公司: Clontech
载体抗性: 氨苄霉素
空载体大小: 6300 bp
载体类型: 哺乳细胞表达
筛选标记: 嘌呤霉素
高拷贝/低拷贝: 未知
生长条件: 37 ℃

插入基因信息
插入基因名称: Peredox-mCherry-NLS
插入大小: 2800 bp
标签/融合蛋白: Nuclear Localization Seq(C端)
克隆方法: 限制性内切酶
5' 克隆位点: XhoI(未破坏)
3' 克隆位点: EcoRI(克隆期间被破坏)
5' 测序引物 CCCTTGAACCTCCTCGTTCGACC
3' 测序引物 GAGACGTGCTACTTCCATTTGTC

文献引用方法
材料和方法部分: pMSCV-Peredox-mCherry-NLS was a gift from Gary Yellen (Addgene plasmid # 32385)
参考文献部分: Imaging Cytosolic NADH-NAD(+) Redox State with a Genetically Encoded Fluorescent Biosensor. Hung YP, Albeck JG, Tantama M, Yellen G. Cell Metab. 2011 Oct 5;14(4):545-54. 10.1016/j.cmet.2011.08.012 PubMed 21982714

质粒图谱信息

pMSCV-Peredox-mCherry-NLS载体图谱

质粒简介

质粒序列

LOCUS       pMSCV-Peredox-mCherry-NLS	9107 bp 	DNA	SYN
DEFINITION  pMSCV-Peredox-mCherry-NLS
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..9107
                     /organism="pMSCV-Peredox-mCherry-NLS"
                     /mol_type="other DNA"
     misc_feature    654..1403
                     /label="psi_plus_pack"
     misc_feature    990..1397
                     /label="gag"
     misc_feature    1333..1355
                     /label="MSCV_primer"
     misc_feature    1333..1355
                     /label="pLXSN_5_primer"
     misc_feature    1371..1387
                     /label="pBABE_5_primer"
     CDS             complement(1373..2074)
                     /label="ORF frame 2"
     CDS             1433..4231
                     /label="ORF frame 2"
     CDS             complement(2246..2797)
                     /label="ORF frame 2"
     CDS             complement(2995..3558)
                     /label="ORF frame 3"
     CDS             complement(3290..4357)
                     /label="ORF frame 2"
     gene            3494..4198
                     /label="mCherry"
                     /gene="mCherry"
     misc_feature    3671..3724
                     /label="Q66M (mCherry)"
     misc_feature    4199..4219
                     /label="SV40 NLS"
     misc_feature    4205..4219
                     /label="NLS"
     misc_feature    complement(4285..4308)
                     /label="MSCV_rev_primer"
     CDS             4530..5369
                     /label="ORF frame 3"
     misc_feature    4682..4701
                     /label="mPGK_F_primer"
     CDS             complement(4695..5420)
                     /label="ORF frame 1"
     gene            4770..5369
                     /label="puro (variant)"
                     /gene="puro (variant)"
     promoter        complement(6148..6166)
                     /label="M13_reverse_primer"
     misc_feature    complement(6165..6187)
                     /label="M13_pUC_rev_primer"
     promoter        complement(6201..6230)
                     /label="lac_promoter"
     gene            complement(7313..8173)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(7313..8173)
                     /label="ORF frame 2"
     promoter        complement(8215..8243)
                     /label="AmpR_promoter"
     misc_feature    complement(8402..8424)
                     /label="pGEX_3_primer"
     misc_feature    complement(8623..8754)
                     /label="lacZ_a"
     misc_feature    8737..8759
                     /label="M13_pUC_fwd_primer"
     misc_feature    8887..8906
                     /label="pBRrevBam_primer"
ORIGIN
    1 TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT TGCAAGGCAT
   61 GGAAAATACA TAACTGAGAA TAGAGAAGTT CAGATCAAGG TTAGGAACAG AGAGACAGCA
  121 GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA
  181 ACAGATGGTC CCCAGATGCG GTCCCGCCCT CAGCAGTTTC TAGAGAACCA TCAGATGTTT
  241 CCAGGGTGCC CCAAGGACCT GAAATGACCC TGTGCCTTAT TTGAACTAAC CAATCAGTTC
  301 GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT CCCCGAGCTC AATAAAAGAG CCCACAACCC
  361 CTCACTCGGC GCGCCAGTCC TCCGATAGAC TGCGTCGCCC GGGTACCCGT ATTCCCAATA
  421 AAGCCTCTTG CTGTTTGCAT CCGAATCGTG GACTCGCTGA TCCTTGGGAG GGTCTCCTCA
  481 GATTGATTGA CTGCCCACCT CGGGGGTCTT TCATTTGGAG GTTCCACCGA GATTTGGAGA
  541 CCCCTGCCCA GGGACCACCG ACCCCCCCGC CGGGAGGTAA GCTGGCCAGC GGTCGTTTCG
  601 TGTCTGTCTC TGTCTTTGTG CGTGTTTGTG CCGGCATCTA ATGTTTGCGC CTGCGTCTGT
  661 ACTAGTTAGC TAACTAGCTC TGTATCTGGC GGACCCGTGG TGGAACTGAC GAGTTCTGAA
  721 CACCCGGCCG CAACCCTGGG AGACGTCCCA GGGACTTTGG GGGCCGTTTT TGTGGCCCGA
  781 CCTGAGGAAG GGAGTCGATG TGGAATCCGA CCCCGTCAGG ATATGTGGTT CTGGTAGGAG
  841 ACGAGAACCT AAAACAGTTC CCGCCTCCGT CTGAATTTTT GCTTTCGGTT TGGAACCGAA
  901 GCCGCGCGTC TTGTCTGCTG CAGCGCTGCA GCATCGTTCT GTGTTGTCTC TGTCTGACTG
  961 TGTTTCTGTA TTTGTCTGAA AATTAGGGCC AGACTGTTAC CACTCCCTTA AGTTTGACCT
 1021 TAGGTCACTG GAAAGATGTC GAGCGGATCG CTCACAACCA GTCGGTAGAT GTCAAGAAGA
 1081 GACGTTGGGT TACCTTCTGC TCTGCAGAAT GGCCAACCTT TAACGTCGGA TGGCCGCGAG
 1141 ACGGCACCTT TAACCGAGAC CTCATCACCC AGGTTAAGAT CAAGGTCTTT TCACCTGGCC
 1201 CGCATGGACA CCCAGACCAG GTCCCCTACA TCGTGACCTG GGAAGCCTTG GCTTTTGACC
 1261 CCCCTCCCTG GGTCAAGCCC TTTGTACACC CTAAGCCTCC GCCTCCTCTT CCTCCATCCG
 1321 CCCCGTCTCT CCCCCTTGAA CCTCCTCGTT CGACCCCGCC TCGATCCTCC CTTTATCCAG
 1381 CCCTCACTCC TTCTCTAGGC GCCGGAATTA GATCTCTCGA GTTGGGCCCA CCATGGTTGC
 1441 TAGCAAAGTT CCTGAAGCAG CCATTTCCAG ACTGATTACT TACCTGAGAA TTCTGGAAGA
 1501 GCTGGAAGCC CAAGGTGTAC ACCGCACCGC CTCCGAGCAA CTGGGCGAGC TGGCCCAGGT
 1561 CACCGCCTTC CAGGTTGATA AGGACCTGTC CTACTTCGGC TCCTACGGCA CTGACGGCGT
 1621 TGGCTACACT GTACCGGTCC TCAAGAGAGA ACTGCGCCAT ATCCTCGGTC TGAACCGCAA
 1681 ATGGGGCCTG TGCATCGTGG GGATGGGCCG CCTGGGATCC GCTCTTGCTG ATTGGCCTGG
 1741 TTTTGGCGAG AGCTTCGAGC TGCGCGGTTT CTTCGATGTT GACCCAGGTA TGGTTGGTCG
 1801 GCCGGTTCGC GGTGGTGTTA TCGAACACGT TGATCTGCTG CCCCAGCGCG TACCTGGTCG
 1861 CATCGAAATC GCTCTGCTGA CGGTTCCACG CGAGGCAGCA CAGAAGGCTG CCGACCTGCT
 1921 GGTTGCCGCT GGCATCAAGG GCATCCTGAA CTTCGCTCCA GTTGTACTGG AGGTTCCCAA
 1981 GGAAGTGGCT GTTGAGAACG TTGACATCCT GGCCGGTCTG ACGCGTCTGA GCTTTGCCAT
 2041 TCTGAACCCC ACGTGGAGCG CAGCAGGTGG GCATGGTTTC ACTGCTCACA ACGTGTATAT
 2101 CATGGCTGAC AAACAGAAGA ACGGTATCAA GGCCAACTTC AAGATCCGCC ACAACATCGA
 2161 AGACGGCGGC GTTCAGCTGG CTGATCACTA CCAGCAGAAC ACCCCAATCG GCGACGGCCC
 2221 TGTTCTGCTG CCTGACAACC ACTACTTAAG CATCCAGTCT AAACTGTCCA AAGACCCAAA
 2281 CGAGAAGCGC GACCACATGG TTCTGCTGGA ATTTGTTACC GCCGCTGGCA TCACCCACGG
 2341 CATGGACGAA CTGTACAAGG GCGGCACCGG GGGATCTATG GTTTCCAAAG GCGAGGAACT
 2401 GTTCACTGGT GTTGTTCCTA TCCTGGTTGA ACTGGACGGC GACGTTAACG GCCACAAATT
 2461 CAGCGTTAGC GGGGAAGGCG AAGGAGACGC TACCTACGGC AAGCTGACTC TGAAGTTCAT
 2521 CTGCACCACT GGCAAGCTGC CTGTTCCTTG GCCTACCCTG GTTACTACCT TCTCCTATGG
 2581 TGTTATGGTT TTCGCCAGAT ATCCTGACCA CATGAAGCAG CACGACTTCT TCAAGAGCGC
 2641 CATGCCTGAG GGCTACGTTC AGGAACGCAC CATCTTTTTC AAGGACGACG GCAACTATAA
 2701 GACACGCGCT GAGGTTAAGT TCGAGGGCGA CACTCTGGTT AACCGCATCG AGCTGAAGGG
 2761 CATCGACTTC AAGGAGGACG GCAACATCCT GGGCCATAAG CTTGAATATA ACACCAAGGT
 2821 ACCTGAAGCC GCCATCAGCC GCCTCATCAC TTACCTCCGC ATCCTGGAGG AGCTCGAAGC
 2881 CCAAGGCGTT CACCGCACTG CAAGCGAGCA GCTGGGCGAA CTGGCACAGG TTACAGCATT
 2941 CCAGGTTGAC GAAGATCTGT CCTATTTCGG TTCCTACGGC ACCGACGGTG TTGGCTACAC
 3001 AGTCCCTGTC CTGAAGCGCG AGCTGCGCCA CATCCTGGGC CTGAACCGCA AGTGGGGCTT
 3061 ATGCATCGTT GGCATGGGCC GCCTCGGTAG TGCACTGGCA GACTGGCCGG GCTTTGGTGA
 3121 ATCTTTCGAA CTGCGCGGCT TCTTCGACGT TGATCCAGGC ATGGTTGGGC GCCCTGTTCG
 3181 CGGTGGCGTT ATCGAGCATG TTGACCTCCT GCCACAGCGC GTCCCGGGTC GCATCGAGAT
 3241 CGCCCTGCTG ACCGTTCCTC GCGAAGCTGC ACAGAAGGCA GCCGACTTAC TAGTCGCCGC
 3301 TGGTATCAAG GGAATTCTGA ACTTCGCCCC TGTTGTTCTG GAGGTTCCTA AGGAAGTTGC
 3361 TGTTGAAAAC GTTGATTTCC TGGCCGGCCT GACCCGCCTG AGCTTCGCAA TCCTGAATCC
 3421 TAAGTGGCGC GAAGAAATGA TGGGTTCCGG AACTGGGGGC AACGCCTCTG ACGGTGGTGG
 3481 GTCTGGTGGT ATGGTGAGCA AGGGCGAGGA GGATAACATG GCCATCATCA AGGAGTTCAT
 3541 GCGCTTCAAG GTGCACATGG AGGGCTCCGT GAACGGCCAC GAGTTCGAGA TCGAGGGCGA
 3601 GGGCGAGGGC CGCCCCTACG AGGGCACCCA GACCGCCAAG CTGAAGGTGA CCAAGGGTGG
 3661 CCCCCTGCCC TTCGCCTGGG ACATCCTGTC CCCTCAGTTC ATGTACGGCT CCAAGGCCTA
 3721 CGTGAAGCAC CCCGCCGACA TCCCCGACTA CTTGAAGCTG TCCTTCCCCG AGGGCTTCAA
 3781 GTGGGAGCGC GTGATGAACT TCGAGGACGG CGGCGTGGTG ACCGTGACCC AGGACTCCTC
 3841 CCTGCAGGAC GGCGAGTTCA TCTACAAGGT GAAGCTGCGC GGCACCAACT TCCCCTCCGA
 3901 CGGCCCCGTA ATGCAGAAGA AGACCATGGG CTGGGAGGCC TCCTCCGAGC GGATGTACCC
 3961 CGAGGACGGC GCCCTGAAGG GCGAGATCAA GCAGAGGCTG AAGCTGAAGG ACGGCGGCCA
 4021 CTACGACGCT GAGGTCAAGA CCACCTACAA GGCCAAGAAG CCCGTGCAGC TGCCCGGCGC
 4081 CTACAACGTC AACATCAAGT TGGACATCAC CTCCCACAAC GAGGACTACA CCATCGTGGA
 4141 ACAGTACGAA CGCGCCGAGG GCCGCCACTC CACCGGCGGC ATGGACGAAC TGTACAAGCC
 4201 TAAAAAGAAA CGTAAAGTTG AAGATGCTTG AGTCGACTAC AATTCTACCG GGTAGGGGAG
 4261 GCGCTTTTCC CAAGGCAGTC TGGAGCATGC GCTTTAGCAG CCCCGCTGGG CACTTGGCGC
 4321 TACACAAGTG GCCTCTGGCC TCGCACACAT TCCACATCCA CCGGTAGGCG CCAACCGGCT
 4381 CCGTTCTTTG GTGGCCCCTT CGCGCCACCT TCTACTCCTC CCCTAGTCAG GAAGTTCCCC
 4441 CCCGCCCCGC AGCTCGCGTC GTGCAGGACG TGACAAATGG AAGTAGCACG TCTCACTAGT
 4501 CTCGTGCAGA TGGACAGCAC CGCTGAGCAA TGGAAGCGGG TAGGCCTTTG GGGCAGCGGC
 4561 CAATAGCAGC TTTGCTCCTT CGCTTTCTGG GCTCAGAGGC TGGGAAGGGG TGGGTCCGGG
 4621 GGCGGGCTCA GGGGCGGGCT CAGGGGCGGG GCGGGCGCCC GAAGGTCCTC CGGAGGCCCG
 4681 GCATTCTGCA CGCTTCAAAA GCGCACGTCT GCCGCGCTGT TCTCCTCTTC CTCATCTCCG
 4741 GGCCTTTCGA CCTGCAGCCC AAGCTTACCA TGACCGAGTA CAAGCCCACG GTGCGCCTCG
 4801 CCACCCGCGA CGACGTCCCC AGGGCCGTAC GCACCCTCGC CGCCGCGTTC GCCGACTACC
 4861 CCGCCACGCG CCACACCGTC GATCCGGACC GCCACATCGA GCGGGTCACC GAGCTGCAAG
 4921 AACTCTTCCT CACGCGCGTC GGGCTCGACA TCGGCAAGGT GTGGGTCGCG GACGACGGCG
 4981 CCGCGGTGGC GGTCTGGACC ACGCCGGAGA GCGTCGAAGC GGGGGCGGTG TTCGCCGAGA
 5041 TCGGCCCGCG CATGGCCGAG TTGAGCGGTT CCCGGCTGGC CGCGCAGCAA CAGATGGAAG
 5101 GCCTCCTGGC GCCGCACCGG CCCAAGGAGC CCGCGTGGTT CCTGGCCACC GTCGGCGTCT
 5161 CGCCCGACCA CCAGGGCAAG GGTCTGGGCA GCGCCGTCGT GCTCCCCGGA GTGGAGGCGG
 5221 CCGAGCGCGC CGGGGTGCCC GCCTTCCTGG AGACCTCCGC GCCCCGCAAC CTCCCCTTCT
 5281 ACGAGCGGCT CGGCTTCACC GTCACCGCCG ACGTCGAGGT GCCCGAAGGA CCGCGCACCT
 5341 GGTGCATGAC CCGCAAGCCC GGTGCCTGAC GCCCGCCCCA CGACCCGCAG CGCCCGACCG
 5401 AAAGGAGCGC ACGACCCCAT GCATCGATAA AATAAAAGAT TTTATTTAGT CTCCAGAAAA
 5461 AGGGGGGAAT GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT
 5521 GCAAGGCATG GAAAATACAT AACTGAGAAT AGAGAAGTTC AGATCAAGGT TAGGAACAGA
 5581 GAGACAGCAG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA
 5641 GGGCCAAGAA CAGATGGTCC CCAGATGCGG TCCCGCCCTC AGCAGTTTCT AGAGAACCAT
 5701 CAGATGTTTC CAGGGTGCCC CAAGGACCTG AAATGACCCT GTGCCTTATT TGAACTAACC
 5761 AATCAGTTCG CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC CCCGAGCTCA ATAAAAGAGC
 5821 CCACAACCCC TCACTCGGCG CGCCAGTCCT CCGATAGACT GCGTCGCCCG GGTACCCGTG
 5881 TATCCAATAA ACCCTCTTGC AGTTGCATCC GACTTGTGGT CTCGCTGTTC CTTGGGAGGG
 5941 TCTCCTCTGA GTGATTGACT ACCCGTCAGC GGGGGTCTTT CATGGGTAAC AGTTTCTTGA
 6001 AGTTGGAGAA CAACATTCTG AGGGTAGGAG TCGAATATTA AGTAATCCTG ACTCAATTAG
 6061 CCACTGTTTT GAATCCACAT ACTCCAATAC TCCTGAAATA GTTCATTATG GACAGCGCAG
 6121 AAGAGCTGGG GAGAATTAAT TCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT
 6181 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG
 6241 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG
 6301 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC
 6361 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC
 6421 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA
 6481 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG
 6541 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT
 6601 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA
 6661 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC
 6721 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT
 6781 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG
 6841 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG
 6901 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT
 6961 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC
 7021 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG
 7081 CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC
 7141 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT
 7201 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA
 7261 AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT
 7321 GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT
 7381 GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG
 7441 CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG
 7501 CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA
 7561 ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG
 7621 CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG
 7681 GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT
 7741 CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA
 7801 TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG
 7861 GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC
 7921 CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG
 7981 GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA
 8041 TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG
 8101 GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT
 8161 GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC
 8221 TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA
 8281 CATTTCCCCG AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT
 8341 ATAAAAATAG GCGTATCACG AGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA
 8401 ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA
 8461 GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT
 8521 ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA
 8581 GATGCGTAAG GAGAAAATAC CGCATCAGGC GCCATTCGCC ATTCAGGCTG CGCAACTGTT
 8641 GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG
 8701 CTGCAAGGCG ATTAAGTTGG GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA
 8761 CGGCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC
 8821 TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC
 8881 CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC
 8941 GGCCACGATG CGTCCGGCGT AGAGGCGATT AGTCCAATTT GTTAAAGACA GGATATCAGT
 9001 GGTCCAGGCT CTAGTTTTGA CTCAACAATA TCACCAGCTG AAGCCTATAG AGTACGAGCC
 9061 ATAGATAAAA TAAAAGATTT TATTTAGTCT CCAGAAAAAG GGGGGAA
//

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