| 质粒名称: | PL-SIN-Oct4-EGFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 21319 |
| 存储实验室: | James Ellis |
|
载体骨架基本信息 |
|
| 载体名称: | PL-SIN lentiviral vector |
| 载体出品公司: | Buzina et al., PLoS Genetics, 2008 |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 6531 bp |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Enhanced Green Fluorescence Protein |
| 插入大小: | 720 bp |
| 启动子 | Oct-4 promoter region |
| 克隆方法: | 未知 |
| 5' 克隆位点: | |
| 3' 克隆位点: | |
| 5' 测序引物 | n/a |
| 3' 测序引物 | n/a |
|
文献引用方法 |
|
| 材料和方法部分: | PL-SIN-Oct4-EGFP was a gift from James Ellis (Addgene plasmid # 21319) |
| 参考文献部分: | Isolation of human iPS cells using EOS lentiviral vectors to select for pluripotency. Hotta A, Cheung AY, Farra N, Vijayaragavan K, Seguin CA, Draper JS, Pasceri P, Maksakova IA, Mager DL, Rossant J, Bhatia M, Ellis J. Nat Methods. 2009 May . 6(5):370-6. 10.1038/nmeth.1325 PubMed 19404254 |
PL-SIN-Oct4-EGFP
价格：1200元
联系方式：I47-825O-882O
买家导航
质粒基本信息
质粒图谱信息
质粒简介
质粒序列
LOCUS PL-SIN-Oct4-EGFP 8324 bp DNA SYN
DEFINITION PL-SIN-Oct4-EGFP
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..8324
/organism="PL-SIN-Oct4-EGFP"
/mol_type="other DNA"
misc_feature complement(29..51)
/label="pGEX_3_primer"
misc_feature complement(250..398)
/label="lacZ_a"
misc_feature 364..386
/label="M13_pUC_fwd_primer"
promoter 379..395
/label="M13_forward20_primer"
misc_feature 862..1042
/label="HIV-1_5_LTR"
misc_feature 862..1042
/label="truncHIV-1_3_LTR"
misc_feature 1153..1197
/label="HIV-1_psi_pack"
CDS 1198..1659
/label="ORF frame 1"
misc_feature 1697..1712
/label="cPPT"
CDS 2078..2767
/label="ORF frame 2"
misc_feature 2200..2433
/label="RRE"
CDS complement(4233..4808)
/label="ORF frame 1"
CDS 4708..5484
/label="ORF frame 1"
gene 4768..5481
/label="EGFP"
/gene="EGFP"
misc_feature complement(4810..4831)
/label="EGFP_N_primer"
misc_feature 4948..4977
/label="Y66 (EGFP)"
misc_feature 5418..5439
/label="EGFP_C_primer"
misc_feature 5491..5507
/label="pBluescriptSK_primer"
misc_feature 5594..5609
/label="cPPT"
misc_feature 5594..5615
/label="U3PPT"
misc_feature 5664..5844
/label="truncHIV-1_3_LTR"
misc_feature 5664..5844
/label="HIV-1_5_LTR"
misc_feature complement(5881..5903)
/label="M13_pUC_rev_primer"
promoter complement(5917..5946)
/label="lac_promoter"
rep_origin complement(6255..6874)
/label="pBR322_origin"
gene complement(7029..7888)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(7313..7888)
/label="ORF frame 2"
promoter complement(7930..7958)
/label="AmpR_promoter"
misc_feature complement(8098..8117)
/label="SV40pro_F_primer"
rep_origin complement(8102..8179)
/label="SV40_origin"
ORIGIN
1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC
241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT
301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT
361 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT GCGGCCGCTG GAAGGGCTAA
421 TTTGGTCCCA AAAAAGACAA GAGATCCTTG ATCTGTGGAT CTACCACACA CAAGGCTACT
481 TCCCTGATTG GCAGAACTAC ACACCAGGGC CAGGGATCAG ATATCCACTG ACCTTTGGAT
541 GGTGCTTCAA GTTAGTACCA GTTGAACCAG AGCAAGTAGA AGAGGCCAAA TAAGGAGAGA
601 AGAACAGCTT GTTACACCCT ATGAGCCAGC ATGGGATGGA GGACCCGGAG GGAGAAGTAT
661 TAGTGTGGAA GTTTGACAGC CTCCTAGCAT TTCGTCACAT GGCCCGAGAG CTGCATCCGG
721 AGTACTACAA AGACTGCTGA CATCGAGCTT TCTACAAGGG ACTTTCCGCT GGGGACTTTC
781 CAGGGAGGTG TGGCCTGGGC GGGACTGGGG AGTGGCGAGC CCTCAGATGC TACATATAAG
841 CAGCTGCTTT TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC
901 TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTCAAA
961 GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG
1021 TCAGTGTGGA AAATCTCTAG CAGTGGCGCC CGAACAGGGA CTTGAAAGCG AAAGTAAAGC
1081 CAGAGGAGAT CTCTCGACGC AGGACTCGGC TTGCTGAAGC GCGCACGGCA AGAGGCGAGG
1141 GGCGGCGACT GGTGAGTACG CCAAAAATTT TGACTAGCGG AGGCTAGAAG GAGAGAGATG
1201 GGTGCGAGAG CGTCGGTATT AAGCGGGGGA GAATTAGATA AATGGGAAAA AATTCGGTTA
1261 AGGCCAGGGG GAAAGAAACA ATATAAACTA AAACATATAG TATGGGCAAG CAGGGAGCTA
1321 GAACGATTCG CAGTTAATCC TGGCCTTTTA GAGACATCAG AAGGCTGTAG ACAAATACTG
1381 GGACAGCTAC AACCATCCCT TCAGACAGGA TCAGAAGAAC TTAGATCATT ATATAATACA
1441 ATAGCAGTCC TCTATTGTGT GCATCAAAGG ATAGATGTAA AAGACACCAA GGAAGCCTTA
1501 GATAAGATAG AGGAAGAGCA AAACAAAAGT AAGAAAAAGG CACAGCAAGC AGCAGCTGAC
1561 ACAGGAAACA ACAGCCAGGT CAGCCAAAAT TACCCTATAG TGCAGAACCT CCAGGGGCAA
1621 ATGGTACATC AGGCCATATC ACCTAGAACT TTAAATTAAG ACAGCAGTAC AAATGGCAGT
1681 ATTCATCCAC AATTTTAAAA GAAAAGGGGG GATTGGGGGG TACAGTGCAG GGGAAAGAAT
1741 AGTAGACATA ATAGCAACAG ACATACAAAC TAAAGAATTA CAAAAACAAA TTACAAAAAT
1801 TCAAAATTTT CGGGTTTATT ACAGGGACAG CAGAGATCCA GTTTGGAAAG GACCAGCAAA
1861 GCTCCTCTGG AAAGGTGAAG GGGCAGTAGT AATACAAGAT AATAGTGACA TAAAAGTAGT
1921 GCCAAGAAGA AAAGCAAAGA TCATCAGGGA TTATGGAAAA CAGATGGCAG GTGATGATTG
1981 TGTGGCAAGT AGACAGGATG AGGATTAACA CATGGAAAAG ATTAGTAAAA CACCATAGCT
2041 CTAGAGCGAT CCGATCTTCA GACCTGGAGG AGGCGATATG AGGGACAATT GGAGAAGTGA
2101 ATTATATAAA TATAAAGTAG TAAAAATTGA ACCATTAGGA GTAGCACCCA CCAAGGCAAA
2161 GAGAAGAGTG GTGCAGAGAG AAAAAAGAGC AGTGGGAATA GGAGCTTTGT TCCTTGGGTT
2221 CTTGGGAGCA GCAGGAAGCA CTATGGGCTG CACGTCAATG ACGCTGACGG TACAGGCCAG
2281 ACAATTATTG TCTGATATAG TGCAGCAGCA GAACAATTTG CTGAGGGCTA TTGAGGCGCA
2341 ACAGCATCTG TTGCAACTCA CAGTCTGGGG CATCAAACAG CTCCAGGCAA GAATCCTGGC
2401 TGTGGAAAGA TACCTAAAGG ATCAACAGCT CCTGGGGATT TGGGGTTGCT CTGGAAAACT
2461 CATTTGCACC ACTGCTGTGC CTTGGAATGC TAGTTGGAGT AATAAATCTC TGGAACAGAT
2521 TTGGAATAAC ATGACCTGGA TGGAGTGGGA CAGAGAAATT AACAATTACA CAAGCTTGGT
2581 AGGTTTAAGA ATAGTTTTTG CTGTACTTTC TATAGTGAAT AGAGTTAGGC AGGGATATTC
2641 ACCATTATCG TTTCAGACCC ACCTCCCAAT CCCGAGGGGA CCCGACAGGC CCGAAGGAAT
2701 AGAAGAAGAA GGTGGAGAGA GAGACAGAGA CAGATCCATT CGATTAGTGA ACGGATCAGC
2761 TTTGTGAACT TGGCGGCTTC CAAGTCGCTG CCTTTATTTA GGTCTTCCAA CTAACCTATG
2821 GCACTGTTCC ACAATGAATG TATAGAAATT GGGAGGTGAG CATGACAGAG TGGAGGAAAC
2881 GGAAGATTCA TGGAGAGGGC CAGAGAGATG GCCCCTCAGC CACCCTGGGG GATGACTTGG
2941 ACCCATGTGG TAGAAGGAGG GGACTTCCAC ACATGTGCTA TGTGTAGCTG TGTGTAGGTA
3001 CATACACACC CTTAAAATAA AACGCAATTT TTTTTTCAAA GTCTCAGGGT GAATTTGGTG
3061 AAGTCGATGA AGCTGAGGCA GGAGAATTAT CAGGAGTTCA AGGGCAGCTT GTTTTATAGA
3121 GAAAGGTTCC ATCTCTACCT GATGAAGACT ACCATCAAGA GACACCCCCG CCCCCCAGGG
3181 CACCTAGAGC CACTGACCCT AGCCAACAGC TCAGGCGGGC TGGGCCCAGG CTCAGAACTC
3241 TGTCCTGGCT ATGTACACTG TGGGGTGCTC TGGGCTTTTT GAGGCTGTGT GATTCACCCT
3301 GGGGCCTTCG TTCAGAGCAT GGTGTAGGAG CAGACAGACA AACACCATCC CTTGCAGACA
3361 GGCACTCTGA GGGCTATTCT CTTGCAAAGA TAACTAAGCA CCAGGCCAGT AATGGGATCC
3421 TCAGACTGGG CCCAGAAAAC CACTCTAGGG AAGTTCAGGG TAGGCTCTCT GCACCCCCTC
3481 CTCCTAATCC CGTCTCCTTA GTGTCTTTCC GCCAGCACAG GAATGGGGGA GGGGTGGGTG
3541 ACGAGGATGA ACACCGGAGT CCCTGGAGGA AGGGAAGCAG GGTATCTCCA TCTGAGGCTC
3601 TGTCTTTGAG GAGAGGTGGA GAGCTGGGGA AGTCTTGTGT GAGGGGATTG GGGCTCAGGA
3661 GGGGGTTGGG GAGCAGGAAG TTGTCCCCAG GGGAGCCATC CTGGCCCATT CAAGGGTTGA
3721 GTACTTGTTT AGGGTTAGAG CTGCCCCCTC TGGGGACCAG GATTGTCCAG CCAAGGCCAT
3781 TGTCCTGCCC CCTTCCCCCA GTCCCTCCCA GGCCCCTTTG AACCTGAAGT CAGATATTTC
3841 TTCTCTCTAC CCACCTCCCA CCCGTTGGGT TTCTCCACCC AGGAACTAGG CTGGAAGCCT
3901 GGGATGAGGA GGTGGGGGGA GGGAGAACTG AGAATCTTGA GGAAAGAGGC CCCGGCCTTA
3961 ACTGTGAGGG GATGGAGCCT GGGTGCAGGT CTTATGGGGG TTGGGgcGGG TGGTTAGTGT
4021 CTAATCTACC AACCTGGACA ACACAAGATG GAATACTGTG CTCTGAAAAC GCAGAGCCAG
4081 CACTTCTCTG GGGTCTCTGG GGACATATCT GGTTGGGGCT CGGGGTCCCA TGGTGTAGAG
4141 CCTCTAAACT CTGGAGGACT GGAGGTGCAA TGGCTGTCTT GTCCTGGCCT TGGACATGGG
4201 CTGAAATACT GGGTTCACCC ATATCTAGGA CTCTAGACGG GTGGGTAAGC AAGAACTGAG
4261 GAGTGGCCCC AGAAATAATT GGCACACGAA CATTCAATGG ATGTTTTAGG CTCTCCAGAG
4321 GATGGCTGAG TGGGCTGTAA GGACAGGCCG AGAGGGTGCA GTGCCAACAG GCTTTGTGGT
4381 GCGATGGGGC ATCCGAGCAA CTGGTTTGTG AGGTGTCCGG TGACCCAAGG CAGGGGTGAG
4441 AGGACCTTGA AGGTTGAAAA TGAAGGCCTC CTGGGGTCCC GTCCTAAGGG TTGTCCTGTC
4501 CAGACGTCCC CAACCTCCGT CTGGAAGACA CAGGCAGATA GCGCTCGCCT CAGTTTCTCC
4561 CACCCCCACA GCTCTGCTCC TCCACCCACC CAGGGGGCGG GGCCAGAGGT CAAGGCTAGA
4621 GGGTGGGATT GGGGAGGGAG AGGTGAAACC GTCCCTAGGT GAGCCGTCTT TCCACCAGGC
4681 CCCCGGCTCG GGGTGCCCAC CTTCCCCATG ACGCGAATTC TGCAGTCGAC GGTACCGCGG
4741 GCCCGGGATC CACCGGTCGC CACCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG
4801 GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC
4861 GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC
4921 AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGA CCTACGGCGT GCAGTGCTTC
4981 AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC
5041 TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG
5101 GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG
5161 GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT ACAACAGCCA CAACGTCTAT
5221 ATCATGGCCG ACAAGCAGAA GAACGGCATC AAGGTGAACT TCAAGATCCG CCACAACATC
5281 GAGGACGGCA GCGTGCAGCT CGCCGACCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC
5341 CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCACCCAGT CCGCCCTGAG CAAAGACCCC
5401 AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG GATCACTCTC
5461 GGCATGGACG AGCTGTACAA GTAAAGCGAC TCTAGAACTA GTGGATCCCC CGGGCTGCAG
5521 GAATTCGATA TCAAGCTGGG GTACCTTTAA GACCAATGAC TTACAAGGCA GCTGTAGATC
5581 TTAGCCACTT TTTAAAAGAA AAGGGGGGAC TGGAAGGGCT AATTCACTCC CAAAGAAGAC
5641 AAGATCTGCT TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC
5701 TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC
5761 AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT
5821 AGTCAGTGTG GAAAATCTCT AGCAGAATTC TAGCTTGGCG TAATCATGGT CATAGCTGTT
5881 CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG
5941 TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG
6001 CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG
6061 GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC
6121 TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC
6181 ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG
6241 AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT
6301 CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG
6361 GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA
6421 TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCAATGCTC ACGCTGTAGG
6481 TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT
6541 CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC
6601 GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC
6661 GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT
6721 GGTATCTGCG CTCTGCTGAA GCCACTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC
6781 GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC
6841 AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG
6901 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG
6961 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG
7021 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT
7081 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA
7141 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA
7201 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC
7261 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT
7321 TTGCGCAACG TTGTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG
7381 CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA
7441 AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT
7501 TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT
7561 GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC
7621 CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA
7681 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT
7741 TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT
7801 TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA
7861 GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT
7921 ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA
7981 TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGG GACTAGCTTT TTGCAAAAGC
8041 CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT
8101 CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG
8161 GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA
8221 GATGTAGCTT GCATGCCGAC ATGGATTATT GACTAGTCCC TAAGAAACCA TTATTATCAT
8281 GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTC
//
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