| 质粒名称: | phOct4-EGFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 38776 |
| 存储实验室: | Wei Cui |
|
载体骨架基本信息 |
|
| 载体名称: | pEGFP-1 |
| 载体出品公司: | Clontech |
| 载体抗性: | 卡那霉素 |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 未知 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Oct-4 promoter |
| 物种来源: | H. sapiens (人) |
| 插入大小: | 3950 bp |
| 启动子 | human OCT4 |
| 标签/融合蛋白: | EGFP（C端） |
| 克隆方法: | TOPO克隆 |
| 5' 测序引物 | TT CCC ATG TCA AGT AAG TGG GGT GG |
| 3' 测序引物 | CGA GAA GGC AAA ATC TGA AGC CAG G |
|
文献引用方法 |
|
| 材料和方法部分: | phOct4-EGFP was a gift from Wei Cui (Addgene plasmid # 38776) |
| 参考文献部分: | Stably transfected human embryonic stem cell clones express OCT4-specific green fluorescent protein and maintain self-renewal and pluripotency. Gerrard L, Zhao D, Clark AJ, Cui W. Stem Cells. 2005;23(1):124-33. 10.1634/stemcells.2004-0102 PubMed 15625129 |
phOct4-EGFP
价格：1200元
联系方式：I47-825O-882O
买家导航
质粒基本信息
质粒图谱信息
质粒简介
质粒序列
LOCUS phOct4-EGFP 8068 bp DNA SYN
DEFINITION phOct4-EGFP
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..8068
/organism="phOct4-EGFP"
/mol_type="other DNA"
CDS complement(1080..1568)
/label="ORF frame 3"
CDS complement(3017..3487)
/label="ORF frame 1"
CDS 3960..4733
/label="ORF frame 3"
gene 4017..4730
/label="EGFP"
/gene="EGFP"
misc_feature complement(4059..4080)
/label="EGFP_N_primer"
misc_feature 4197..4226
/label="Y66 (EGFP)"
misc_feature 4667..4688
/label="EGFP_C_primer"
misc_feature 4947..4966
/label="EBV_rev_primer"
rep_origin complement(5116..5422)
/label="f1_origin"
promoter 5501..5529
/label="AmpR_promoter"
misc_feature complement(5595..5615)
/label="pBABE_3_primer"
misc_feature complement(5601..5816)
/label="SV40_enhancer"
promoter 5613..5881
/label="SV40_promoter"
rep_origin 5780..5857
/label="SV40_origin"
misc_feature 5842..5861
/label="SV40pro_F_primer"
CDS 5964..6758
/label="ORF frame 3"
gene 5967..6755
/label="NeoR/KanR"
/gene="NeoR/KanR"
CDS complement(6273..6809)
/label="ORF frame 3"
terminator 6933..7202
/label="TK_PA_terminator"
rep_origin 7350..7969
/label="pBR322_origin"
ORIGIN
1 TAGTTATTAC TAGCGCTACC GGACTCAGAT CTCGAGCTCA AGCTTCCCAT GTCAAGTAAG
61 TGGGGTGGGT AGATGGGAGG GCATATGAGA GCGGGGCAGC AATTTTATGT CTTAGTTGGA
121 AGGAAGTTTA GAGTTCATTG AGTCCAAATC CTCTTTACTA GGTGAGGAAA CTGAGGCACA
181 GGAGGGGGAG GTGCCATTAG CCAGGGAGTC AGAGCCAGGA CTTAGAACCT GGTCCATGCC
241 TCCTAGTTTG TTCCTGACCA ATCCTCCCAG CTCACCCACT CCTTTTGCTC TCACCTCTGA
301 CTCCCTACAG GCTACAGCTA GAGAGTTGAC TCCTCTATTT GAGGTAAGAG TGTCTGGATT
361 TGGGATAAGG TGACCTCAGT TTGCAGGTCT CATGGGGGAG ATCAGAGGGT CCTTTGTTGA
421 GAAGAGGGGA CTTGGTCCAC AGTGAGCAAA GGAATTTGTC TAAATTGCTA TTAAGGGAGA
481 TCATCTCCCA AGGTGGGCCA AGCCTGAACC CTCAGCCCTC CATTTCTTCC CTTTCTTTTG
541 CCTTCAGATT GACAGGCCTC GGAAGTCAAA ATAAGTGGTT TCCTAGACCG GGTCGAGAGC
601 AAGTCTCTAT TGGTCCCAAC TGAGTTTTTT CAGCTGGTTT TTCAACCAAA CAGCACCTCA
661 TCTCCCAGTG AGGGGAAGGG AAGGCTGGGC TGAGAGCAGC AAGGCTGCTC ATCTCACCTC
721 TCCCCACCCA GCCATGCCAG CCGCCACACC TGGTGGGGAG AGGTGGGCCT CACCTGGGTC
781 CCCTGGCAGT GCTCTGTGAA GGGTCTTGAC ATTGCACTGT AATAATAAAG GTGTGTGTGA
841 AGTATCTTTT TATGGTGACT TTCTAAAACC CAGGGAATCA TGGGACCAGT TCTGATGACT
901 CAGCCTGGCT TCCAGTCTCC TCCAGGCCCA ACGGTGGCCC CCAGCACTGG TTGGGGCCTG
961 GGAGAGCTGG CCTTGGCTGA AGTGAAGCCA CCTACCCTTC AGGCATAACA GGACAGTGAG
1021 AAGGAAGGAA AGCCTGCCTC AACCTCCCAT CAGCCCTGAG CACCCCAGAA GGGGGCCGGC
1081 TAGGAGTCTA GGCATGCAGG AGGCTGACCC CTGACTGGGC TCATATCCAG CCACAAGGCA
1141 GCCAGGGACC CAGGCACCCA CCCCTTGTCT GCGTCCCTCT CGGGAATGGG CCTCTTGCCC
1201 AGGCCAGAAA TACACCACCT ACAGTACAAA TTATAATCTA AAAACAAGAG GGTGGTGTTG
1261 AGTGGGGAAA TTGGGGAAGG TGTTTTAGGA GCCACTAGGA AAATGGGCAG CAGGGACTCT
1321 CTGGACTGGC TTGGGAAGAG CGCTTTTGGG GAACCTGGAG GATGGCAAGC TGAGAAACAC
1381 TGGTGTGGAG ATTCCAGCCA AATCCCAGGC CTGCCCCTCC CCCTCCTCTG AGAGGCCGTC
1441 TTCTTGGCAG ACAGCAGAGA GATGCATGAC AAAGGTGCCG TGATGGTTCT GTCCTGGGGA
1501 TTGAGATGGC TGGGGAGGGG CCTCCTCCTG TTCCGAAGCA TGTTCCTCCC ACCCCCACCA
1561 GGCCCCATAA TCTACCTGCC TTTTGGGCAG TTAAAGGCCG AGAAGTGAAC ACAGCTGCAA
1621 CCCCACTGCC TTGTAGACCT TCCGGCAGAC CTGTGGCAGG TATTGAAATG CACGCATACA
1681 ATTAGGCTCA AAAAGTCTAC ACAGACAGGA GATGGGCACA CGAACAGAGG CAACATAAGA
1741 GTGGGGGAAA AGTCTCAAAA GACTCACGGA TGCCACCAAG ATGAAGACAG CTGGCCACGG
1801 GACACCCATC CCCTTAGAAG GCAGACAGAG CCACTGACCC CAGCAGACAA GCCCAGGCAG
1861 GGCTGAGCCT GGAGCCTGCA ATGAGAAGCC TTACTTAAGT CGACAGAGGT CAGCGTGCCC
1921 AGTCCAGACC TGGCCTTCTG GCCTTCGAAG CTGTGGGGAG CCCTGGCCCA GAGCCCCCTC
1981 TGGAGCCCCC AGACTTACCC CAGGCCCTCC ACTGAGATCA AGTTTTGGGA GCAGACAGAC
2041 AAACATCATC CCTCACAGAC AGGCATTCCG TTGGCTATTC TCTTGCAAAC AGAATCAAGC
2101 ACTAGACCAG CAGCATGAGC CTCAGGATAC TCAGGCCAGG CCCAGAAAAA CAGACCCTGA
2161 AGGGGAGCTT AGGGCAGCCT TCCTGCACCC CTCCACAAAT CACTCGCCAC CTCCTCTGCG
2221 TCTTTCTGCC AGCCAGCCCC ACTAAACAAA GCACATCCCT CAATCTTCCG GGCTCGGGGA
2281 GGGATGCACG ATGAAGCTGG ACGCCTGAGT CCCCCAGAGG AAGGAGGAAC TAGATACCTA
2341 GGTCCCTGTA GGGGGCCCTT GGTGCCCGTC TGAGGCTCAG TCTTTGAGGG GATTGCAGAG
2401 GGGGGTTGCT GGAGCTCCTT TTAGCGTCTC TGAAGGGGAT TCTGTGTGAG GGGATTGGGA
2461 CTGGGGGGTT GGGGAGCAGG AAGCAGTCCC CAGGGGAGCC ATCCAGGCCC ATTCAAGGGT
2521 TGAGCACTTG TTTAGGGTTA GAGCTGCCCC CTCTGGGGAC CGGGATTGTC CAGCCAAGGC
2581 CATTGTCCTG CCCCCTTCCC CCAGTCCCTC CCAGGCTTCT TTGAACCTGA AGTCAGATAT
2641 TTTTTCTCCA CACCCCCCAC CCCCTGGTTT TCCCCACCCA GGGCCTAGGG CTGGAGGCCT
2701 GGGCCAGGGA GGTGGGGGAG GGAGAACGGG GCCTACCGTG GTATTAGATG TCTGAGTTTT
2761 GGTTGAGAGG GGAGCAAGGA ACCTGATGTG CAGGTTCCAT AGTGGAGGGG GCCCAAAGCG
2821 GGTGTCTTAT CACTCTGTTT CAGCAAAGGT TGGGAAACTG AGGCCCAATC AGTCCAAAGT
2881 CTGGTCCCTT GAAGGGGAAG TAGGGGCCAA CCCCTTAGTC TGTTAGATGA GGAGAGTCTG
2941 GAGTCTGATT CTGGAAGACG GAGGGGTGGG GGGATGGGGG GTGGGGGGAT ATAGCACGGA
3001 GGCCTTGTCT GGCAGTCTAC TCTTGAAGAT GGGGTGAAAT TTGGCAGGCT GGGCAGATGG
3061 TGCCAGGCAC CCAGGCTGCG GGGTGGCTGG ATTTGGCCAG TATCGGGATG GGAATGCCTA
3121 GGATTCTGGA TGGATCGGGG GAAGGCATAA GGGAGCAGCT GGCCATTGTG CTTATGGCTG
3181 TTGATGCATT GAGGGATAGC GCCACACACA CATTCAATAA ATTTGAGGAG CTGAGAGGGT
3241 GACTGGCCCC TGAAGGCACA GTGCCAGAGG TCTGTGGAGA GGGGGTCAAG CACCTGGGTT
3301 CCTGAAGAAC ATGGAGGTGT GGGAGTGATT CCAGACAGCT GGGATGTGCA GAGCCTGAGA
3361 GAGTGCCAGG GAGCGGGTTG GGAGTTGAAA GTTGGGTGTG GTGGCTCACG CCTTTAATCA
3421 TGACACTGGG CGGCAGAGGC GGGAGGATTT CTTGAGGACA GGAATTCAAG ACCAGCCTGG
3481 GTAACATAGC AAGGCCCCAT CTCTACTAAA AATAAAAAAA CTAACAGGGC ACAGTGGTCC
3541 AAGCCTGTAG TCCCAGCCAC TTAGGAGGCT GGAGCAGAAG GATTGCTTTG GCCCAGTAGA
3601 TCGAGGCTAC ATTGAGCCAT CATTGTACTC CACTGCACTC CAGTCTGGGC AACAAAGTGA
3661 GACCCTGTCT TAAAAAATAA AAATAAAAAA AGTTTCTGTG GGGGACCTGC ACTGAGGTCC
3721 TGGAGGGGCG CCAGTTGTGT CTCCCGGTTT TCCCCTTCCA CAGACACCAT TGCCACCACC
3781 ATTAGGCAAA CATCCTTCGC CTCAGTTTCT CCCCCCACCT CCCTCTCCTC CACCCATCCA
3841 GGGGGCGGGG CCAGAGGTCA AGGCTAGTGG GTGGGACTGG GGAGGGAGAG AGGGGTTGAG
3901 TAGTCCCTTC GCAAGCCCTC ATTTCACCAG GCCCCCGGCT TGGGGCGCCT TCCTTCCCCA
3961 TGGCGGGACA CCTGGCTTCA GATTTTGCCT TCTCGGATCC ACCGGTCGCC ACCATGGTGA
4021 GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG
4081 TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC
4141 TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA
4201 CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG
4261 ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG
4321 ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC
4381 GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG
4441 AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA
4501 AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT
4561 ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA
4621 GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG
4681 AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG TAAAGCGGCC
4741 GCGACTCTAG ATCATAATCA GCCATACCAC ATTTGTAGAG GTTTTACTTG CTTTAAAAAA
4801 CCTCCCACAC CTCCCCCTGA ACCTGAAACA TAAAATGAAT GCAATTGTTG TTGTTAACTT
4861 GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA
4921 AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTAAGG
4981 CGTAAATTGT AAGCGTTAAT ATTTTGTTAA AATTCGCGTT AAATTTTTGT TAAATCAGCT
5041 CATTTTTTAA CCAATAGGCC GAAATCGGCA AAATCCCTTA TAAATCAAAA GAATAGACCG
5101 AGATAGGGTT GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC ACTATTAAAG AACGTGGACT
5161 CCAACGTCAA AGGGCGAAAA ACCGTCTATC AGGGCGATGG CCCACTACGT GAACCATCAC
5221 CCTAATCAAG TTTTTTGGGG TCGAGGTGCC GTAAAGCACT AAATCGGAAC CCTAAAGGGA
5281 GCCCCCGATT TAGAGCTTGA CGGGGAAAGC CGGCGAACGT GGCGAGAAAG GAAGGGAAGA
5341 AAGCGAAAGG AGCGGGCGCT AGGGCGCTGG CAAGTGTAGC GGTCACGCTG CGCGTAACCA
5401 CCACACCCGC CGCGCTTAAT GCGCCGCTAC AGGGCGCGTC AGGTGGCACT TTTCGGGGAA
5461 ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA
5521 TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT CCTGAGGCGG
5581 AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC
5641 AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC
5701 AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT
5761 CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC
5821 CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT
5881 ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAG ATCGATCAAG
5941 AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG
6001 CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG
6061 ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC
6121 TGTCCGGTGC CCTGAATGAA CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA
6181 CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC
6241 TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG
6301 TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT
6361 TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG
6421 TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA
6481 GGCTCAAGGC GAGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT
6541 TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG
6601 GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG
6661 GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC
6721 GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT
6781 GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA
6841 TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG
6901 GGATCTCATG CTGGAGTTCT TCGCCCACCC TAGGGGGAGG CTAACTGAAA CACGGAAGGA
6961 GACAATACCG GAAGGAACCC GCGCTATGAC GGCAATAAAA AGACAGAATA AAACGCACGG
7021 TGTTGGGTCG TTTGTTCATA AACGCGGGGT TCGGTCCCAG GGCTGGCACT CTGTCGATAC
7081 CCCACCGAGA CCCCATTGGG GCCAATACGC CCGCGTTTCT TCCTTTTCCC CACCCCACCC
7141 CCCAAGTTCG GGTGAAGGCC CAGGGCTCGC AGCCAACGTC GGGGCGGCAG GCCCTGCCAT
7201 AGCCTCAGGT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG
7261 GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC
7321 GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT
7381 TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT
7441 GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT
7501 ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC
7561 ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA
7621 GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG
7681 CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG
7741 ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG
7801 GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA
7861 CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT
7921 GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG
7981 GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC
8041 TGTGGATAAC CGTATTACCG CCATGCAT
//
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