| 质粒名称: | A3A-PBE-ΔUGI |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 119770 |
| 存储实验室: | Caixia Gao |
|
载体骨架基本信息 |
|
| 载体名称: | pJIT163 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4919 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 植物表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | APOBEC3A-nCas9-ΔUGI-NLS |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | nCas9 has a mutation D10A in wild type SpCas9 |
| GenBank编号 | -- |
| 启动子 | maize Ubiquitin-1 |
| 标签/融合蛋白: | NLS（插入件上的C端子） |
| 克隆方法: | Gibson克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | A3A-PBE-ΔUGI was a gift from Caixia Gao (Addgene plasmid # 119770 ; http://n2t.net/addgene:119770 ; RRID:Addgene_119770) |
| 参考文献部分: | Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A. Zong Y, Song Q, Li C, Jin S, Zhang D, Wang Y, Qiu JL, Gao C. Nat Biotechnol. 2018 Oct 1. pii: nbt.4261. doi: 10.1038/nbt.4261. 10.1038/nbt.4261 PubMed 30272679 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS A3A-PBE-Delta-UGI 9759 bp ds-DNA circular SYN 20-DEC-2018
DEFINITION Conversion of C to T in wheat plants or monocotyledons protoplasts
without uracil glycosylase inhibitor (UGI).
ACCESSION .
VERSION .
KEYWORDS A3A-PBE-Delta-UGI
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9759)
AUTHORS Zong Y, Song Q, Li C, Jin S, Zhang D, Wang Y, Qiu JL, Gao C
TITLE Efficient C-to-T base editing in plants using a fusion of nCas9 and
human APOBEC3A.
JOURNAL Nat Biotechnol. 2018 Oct 1. pii: nbt.4261. doi: 10.1038/nbt.4261.
PUBMED 30272679
REFERENCE 2 (bases 1 to 9759)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 20, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9759
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 2669..6769
/codon_start=1
/product="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
/label=Cas9(D10A)
/note="generates RNA-guided single strand nicks in DNA due
to the D10A mutation in the RuvC catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 6770..6817
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
polyA_signal 6875..7051
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
primer_bind complement(7605..7621)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7605..7621)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7618..7640)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7629..7645
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7653..7683)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7698..7719
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7836..7853)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8007..8595)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8087..8106)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8766..9626)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9389..9408
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9627..9731)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gagctcggta cctgacccgg tcgtgcccct ctctagagat aatgagcatt gcatgtctaa
61 gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca gtttatctat
121 ctttatacat atatttaaac tttactctac gaataatata atctatagta ctacaataat
181 atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag gacaattgag
241 tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt ctcctttttt
301 tttgcaaata gcttcaccta tataatactt catccatttt attagtacat ccatttaggg
361 tttagggtta atggttttta tagactaatt tttttagtac atctatttta ttctatttta
421 gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata atttagatat
481 aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga aattaaaaaa
541 actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa cgccgtcgac
601 gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac
661 ggcacggcat ctctgtcgct gcctctggac ccctctcgat cgagagttcc gctccaccgt
721 tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg
781 cacggcaggc ggcctcctcc tcctctcacg gcaccggcag ctacggggga ttcctttccc
841 accgctcctt cgctttccct tcctcgcccg ccgtaataaa tagacacccc ctccacaccc
901 tctttcccca acctcgtgtt gttcggagcg cacacacaca caaccagatc tcccccaaat
961 ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc ccccctctct
1021 accttctcta gatcggcgtt ccggtccatg gttagggccc ggtagttcta cttctgttca
1081 tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc
1141 gacctgtacg tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc
1201 tgggatggct ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg
1261 catagggttt ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg
1321 gtcatctttt catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg
1381 ttctagatcg gagtagaatt aattctgttt caaactacct ggtggattta ttaattttgg
1441 atctgtatgt gtgtgccata catattcata gttacgaatt gaagatgatg gatggaaata
1501 tcgatctagg ataggtatac atgttgatgc gggttttact gatgcatata cagagatgct
1561 ttttgttcgc ttggttgtga tgatgtggtg tggttgggcg gtcgttcatt cgttctagat
1621 cggagtagaa tactgtttca aactacctgg tgtatttatt aattttggaa ctgtatgtgt
1681 gtgtcataca tcttcatagt tacgagttta agatggatgg aaatatcgat ctaggatagg
1741 tatacatgtt gatgtgggtt ttactgatgc atatacatga tggcatatgc agcatctatt
1801 catatgctct aaccttgagt acctatctat tataataaac aagtatgttt tataattatt
1861 ttgatcttga tatacttgga tgatggcata tgcagcagct atatgtggat ttttttagcc
1921 ctgccttcat acgctattta tttgcttggt actgtttctt ttgtcgatgc tcaccctgtt
1981 gtttggtgtt acttctgcaa agcttgtcga cggatccatg gaggccagcc cggctagcgg
2041 cccaaggcat ctcatggacc cgcacatctt caccagcaac ttcaacaacg gcatcggcag
2101 gcacaagacc tacttgtgct acgaggtgga gaggctcgac aacggaacct ccgtgaagat
2161 ggaccaacac agggggttcc tccacaacca agccaagaac ctcctctgcg gcttctacgg
2221 caggcacgcc gagttgaggt tcctcgactt ggtgccatcc ctccaactcg atccagccca
2281 aatctaccgc gtgacctggt tcatctcctg gtccccatgc ttctcctggg gttgcgccgg
2341 cgaggttcgg gctttcctcc aagaaaacac ccacgtccgc ctccgcattt tcgccgccag
2401 gatctatgat tacgaccctc tctacaagga ggccctccag atgctgcggg acgccggtgc
2461 tcaggtgagt atcatgacct acgacgagtt caagcactgc tgggacacct tcgttgacca
2521 ccagggctgc ccattccaac catgggacgg tctggatgaa cacagccaag ccttgtccgg
2581 caggctccgg gccatcctcc aaaaccaggg gaactccggg agcgagacgc caggcacctc
2641 cgagtcggcc accccagaat ctcttaagga caagaagtac tcgatcggcc tcgccatcgg
2701 gacgaactca gttggctggg ccgtgatcac cgacgagtac aaggtgccct ctaagaagtt
2761 caaggtcctg gggaacaccg accgccattc catcaagaag aacctcatcg gcgctctcct
2821 gttcgacagc ggggagaccg ctgaggctac gaggctcaag agaaccgcta ggcgccggta
2881 cacgagaagg aagaacagga tctgctacct ccaagagatt ttctccaacg agatggccaa
2941 ggttgacgat tcattcttcc accgcctgga ggagtctttc ctcgtggagg aggataagaa
3001 gcacgagcgg catcccatct tcggcaacat cgtggacgag gttgcctacc acgagaagta
3061 ccctacgatc taccatctgc ggaagaagct cgtggactcc accgataagg cggacctcag
3121 actgatctac ctcgctctgg cccacatgat caagttccgc ggccatttcc tgatcgaggg
3181 ggatctcaac ccagacaaca gcgatgttga caagctgttc atccaactcg tgcagaccta
3241 caaccaactc ttcgaggaga acccgatcaa cgcctctggc gtggacgcga aggctatcct
3301 gtccgcgagg ctctcgaagt ccaggaggct ggagaacctg atcgctcagc tcccaggcga
3361 gaagaagaac ggcctgttcg ggaacctcat cgctctcagc ctggggctca ccccgaactt
3421 caagtcgaac ttcgatctcg ctgaggacgc caagctgcaa ctctccaagg acacctacga
3481 cgatgacctc gataacctcc tggcccagat cggcgatcaa tacgcggacc tgttcctcgc
3541 tgccaagaac ctgtcggacg ccatcctcct gtcagatatc ctccgcgtga acaccgagat
3601 cacgaaggct ccactctctg cctccatgat caagcgctac gacgagcacc atcaggatct
3661 gaccctcctg aaggcgctgg tccgccaaca gctcccggag aagtacaagg agattttctt
3721 cgatcagtcg aagaacggct acgctgggta catcgacggc ggggcctcac aagaggagtt
3781 ctacaagttc atcaagccaa tcctggagaa gatggacggc acggaggagc tcctggtgaa
3841 gctcaacagg gaggacctcc tgcggaagca gagaaccttc gataacggca gcatccccca
3901 ccaaatccat ctcggggagc tgcacgccat cctgagaagg caagaggact tctacccttt
3961 cctcaaggat aaccgggaga agatcgagaa gatcctgacc ttcagaatcc catactacgt
4021 cggccctctc gcgcggggga actcaagatt cgcttggatg acccgcaagt ctgaggagac
4081 catcacgccg tggaacttcg aggaggtggt ggacaagggc gctagcgctc agtcgttcat
4141 cgagaggatg accaacttcg acaagaacct gcccaacgag aaggtgctcc ctaagcactc
4201 gctcctgtac gagtacttca ccgtctacaa cgagctcacg aaggtgaagt acgtcaccga
4261 gggcatgcgc aagccagcgt tcctgtccgg ggagcagaag aaggctatcg tggacctcct
4321 gttcaagacc aaccggaagg tcacggttaa gcaactcaag gaggactact tcaagaagat
4381 cgagtgcttc gattcggtcg agatcagcgg cgttgaggac cgcttcaacg ccagcctcgg
4441 gacctaccac gatctcctga agatcatcaa ggataaggac ttcctggaca acgaggagaa
4501 cgaggatatc ctggaggaca tcgtgctgac cctcacgctg ttcgaggaca gggagatgat
4561 cgaggagcgc ctgaagacgt acgcccatct cttcgatgac aaggtcatga agcaactcaa
4621 gcgccggaga tacaccggct gggggaggct gtcccgcaag ctcatcaacg gcatccggga
4681 caagcagtcc gggaagacca tcctcgactt cctcaagagc gatggcttcg ccaacaggaa
4741 cttcatgcaa ctgatccacg atgacagcct caccttcaag gaggatatcc aaaaggctca
4801 agtgagcggc cagggggact cgctgcacga gcatatcgcg aacctcgctg gctcccccgc
4861 gatcaagaag ggcatcctcc agaccgtgaa ggttgtggac gagctcgtga aggtcatggg
4921 ccggcacaag cctgagaaca tcgtcatcga gatggccaga gagaaccaaa ccacgcagaa
4981 ggggcaaaag aactctaggg agcgcatgaa gcgcatcgag gagggcatca aggagctggg
5041 gtcccaaatc ctcaaggagc acccagtgga gaacacccaa ctgcagaacg agaagctcta
5101 cctgtactac ctccagaacg gcagggatat gtacgtggac caagagctgg atatcaaccg
5161 cctcagcgat tacgacgtcg atcatatcgt tccccagtct ttcctgaagg atgactccat
5221 cgacaacaag gtcctcacca ggtcggacaa gaaccgcggc aagtcagata acgttccatc
5281 tgaggaggtc gttaagaaga tgaagaacta ctggaggcag ctcctgaacg ccaagctgat
5341 cacgcaaagg aagttcgaca acctcaccaa ggctgagaga ggcgggctct cagagctgga
5401 caaggccggc ttcatcaagc ggcagctggt cgagaccaga caaatcacga agcacgttgc
5461 gcaaatcctc gactctcgga tgaacacgaa gtacgatgag aacgacaagc tgatcaggga
5521 ggttaaggtg atcaccctga agtctaagct cgtctccgac ttcaggaagg atttccagtt
5581 ctacaaggtt cgcgagatca acaactacca ccatgcccat gacgcttacc tcaacgctgt
5641 ggtcggcacc gctctgatca agaagtaccc aaagctggag tccgagttcg tgtacgggga
5701 ctacaaggtt tacgatgtgc gcaagatgat cgccaagtcg gagcaagaga tcggcaaggc
5761 taccgccaag tacttcttct actcaaacat catgaacttc ttcaagaccg agatcacgct
5821 ggccaacggc gagatccgga agagaccgct catcgagacc aacggcgaga cgggggagat
5881 cgtgtgggac aagggcaggg atttcgcgac cgtccgcaag gttctctcca tgccccaggt
5941 gaacatcgtc aagaagaccg aggtccaaac gggcgggttc tcaaaggagt ctatcctgcc
6001 taagcggaac agcgacaagc tcatcgccag aaagaaggac tgggacccaa agaagtacgg
6061 cgggttcgac agccctaccg tggcctactc ggtcctggtt gtggcgaagg ttgagaaggg
6121 caagtccaag aagctcaaga gcgtgaagga gctcctgggg atcaccatca tggagaggtc
6181 cagcttcgag aagaacccaa tcgacttcct ggaggccaag ggctacaagg aggtgaagaa
6241 ggacctgatc atcaagctcc cgaagtactc tctcttcgag ctggagaacg gcaggaagag
6301 aatgctggct tccgctggcg agctccagaa ggggaacgag ctcgcgctgc caagcaagta
6361 cgtgaacttc ctctacctgg cttcccacta cgagaagctc aagggcagcc cggaggacaa
6421 cgagcaaaag cagctgttcg tcgagcagca caagcattac ctcgacgaga tcatcgagca
6481 aatctccgag ttcagcaagc gcgtgatcct cgccgacgcg aacctggata aggtcctctc
6541 cgcctacaac aagcaccggg acaagcccat cagagagcaa gcggagaaca tcatccatct
6601 cttcaccctg acgaacctcg gcgctcctgc tgctttcaag tacttcgaca ccacgatcga
6661 tcggaagaga tacacctcca cgaaggaggt cctggacgcg accctcatcc accagtcgat
6721 caccggcctg tacgagacga ggatcgacct ctcacaactc ggcggggata agagacccgc
6781 agcaaccaag aaggcagggc aagcaaagaa gaagaagtag gaattcggta cgctgaaatc
6841 accagtctct ctctacaaat ctatctctct ctattttctc cataaataat gtgtgagtag
6901 tttcccgata agggaaatta gggttcttat agggtttcgc tcatgtgttg agcatataag
6961 aaacccttag tatgtatttg tatttgtaaa atacttctat caataaaatt tctaattcct
7021 aaaaccaaaa tccagtacta aaatccagat ctcctaaagt ccctatagat ctttgtcgtg
7081 aatataaacc agacacgaga cgactaaacc tggagcccag acgccgttcg aagctagaag
7141 taccgcttag gcaggaggcc gttagggaaa agatgctaag gcagggttgg ttacgttgac
7201 tcccccgtag gtttggttta aatatgatga agtggacgga aggaaggagg aagacaagga
7261 aggataaggt tgcaggccct gtgcaaggta agaagatgga aatttgatag aggtacgcta
7321 ctatacttat actatacgct aagggaatgc ttgtatttat accctatacc ccctaataac
7381 cccttatcaa tttaagaaat aatccgcata agcccccgct taaaaattgg tatcagagcc
7441 atgaataggt ctatgaccaa aactcaagag gataaaacct caccaaaata cgaaagagtt
7501 cttaactcta aagataaaag atctttcaag atcaaaacta gttccctcac accggtgacg
7561 gggatcgcat gcgatatctc gagatctagc ttggcgtaat catggtcata gctgtttcct
7621 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
7681 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
7741 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
7801 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
7861 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
7921 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
7981 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
8041 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
8101 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
8161 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
8221 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
8281 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
8341 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
8401 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
8461 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
8521 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
8581 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
8641 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
8701 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
8761 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
8821 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
8881 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
8941 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
9001 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
9061 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
9121 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
9181 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
9241 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
9301 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
9361 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
9421 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
9481 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
9541 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
9601 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
9661 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
9721 ggggttccgc gcacatttcc ccgaaaagtg ccacctgac
//
