| 质粒名称: | mKeima-Red-N1 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 54597 |
| 存储实验室: | Michael Davidson |
|
载体骨架基本信息 |
|
| 载体名称: | mKeima-Red-N1 |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 4750 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | mKeima-Red（主干上的C终端） |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | mKeima-Red-N1 was a gift from Michael Davidson (Addgene plasmid # 54597 ; http://n2t.net/addgene:54597 ; RRID:Addgene_54597) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS mKeima-Red-N1 4681 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Localization: N1 Cloning Vector, Excitation: 440, Emission: 620.
ACCESSION .
VERSION .
KEYWORDS mKeima-Red-N1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4681)
TITLE Michael Davidson Empty Backbones
REFERENCE 2 (bases 1 to 4681)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4681
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 97..400
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 401..604
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 555..575
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
misc_feature 627..707
/label=MCS
/note="multiple cloning site"
regulatory 709..718
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 715..1383
/codon_start=1
/product="monomeric Keima-Red fluorescent protein, also
known as mKeima"
/label=mKeima-Red
/translation="MVSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTV
TKGGPLPFAWDILSPQLQYGSIPFTKYPEDIPDYFKQSFPEGYTWERSMNFEDGAVCTV
SNDSSIQGNCFIYNVKISGENFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALK
LEGGGHYLCEFKSTYKAKKPVRMPGRHEIDRKLDVTSHNRDYTSVEQCEIAIARHSLLG
"
polyA_signal 1505..1626
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1542..1561)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1596..1615
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(1633..2088)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1770..1791)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 1982..2001
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2115..2219
/gene="bla"
/label=AmpR promoter
promoter 2221..2578
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(2244..2264)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2429..2564
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2491..2510
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 2613..3407
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2667..2686)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3277..3296
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(3595..3614)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 3639..3686
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4015..4603
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4504..4523
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 cctgattctg tggataaccg tattaccgcc atgcattagt tattaatagt aatcaattac
61 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
121 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
181 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
241 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
301 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
361 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
421 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
481 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
541 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
601 agctggttta gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag
661 cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg
721 agtgtgatcg ctaaacaaat gacctacaag gtttatatgt caggcacggt caatggacac
781 tactttgagg tcgaaggcga tggaaaagga aagccttacg agggagagca gacagtaaag
841 ctcactgtca ccaagggtgg acctctgcca tttgcttggg atattttatc accacagctt
901 cagtacggaa gcataccatt caccaagtac cctgaagaca tccctgatta tttcaagcag
961 tcattccctg agggatatac atgggagagg agcatgaact ttgaagatgg tgcagtgtgt
1021 actgtcagca atgattccag catccaaggc aactgtttca tctacaatgt caaaatctct
1081 ggtgagaact ttcctcccaa tggacctgtt atgcagaaga agacacaggg ctgggaaccc
1141 agcactgagc gtctctttgc acgagatgga atgctgatag gaaacgatta tatggctctg
1201 aagttggaag gaggtggtca ctatttgtgt gaatttaaat ctacttacaa ggcaaagaag
1261 cctgtgagga tgccagggcg ccacgagatt gaccgcaaac tggatgtaac cagtcacaac
1321 agggattaca catctgttga gcagtgtgaa atagccattg cacgccactc tttgctcggt
1381 taagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc
1441 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
1501 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
1561 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
1621 atcttaaggc gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
1681 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
1741 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
1801 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
1861 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
1921 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
1981 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
2041 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt
2101 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
2161 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc
2221 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
2281 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
2341 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
2401 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
2461 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
2521 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga
2581 tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
2641 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
2701 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
2761 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc
2821 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
2881 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
2941 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
3001 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
3061 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
3121 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg
3181 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
3241 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
3301 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
3361 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
3421 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
3481 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
3541 ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac
3601 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa
3661 aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc
3721 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc
3781 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg
3841 ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta
3901 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
3961 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
4021 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
4081 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
4141 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
4201 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
4261 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
4321 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
4381 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
4441 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
4501 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
4561 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
4621 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
4681 c
//
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