| 质粒名称: | pCiGSΔΨ |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 108312 |
| 存储实验室: | David Russell |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV beta |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 7164 bp |
| 完整质粒大小: | 5954 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | gag,pCiGSΔΨ is a newer version of the pCiGS Gag expression plasmid with a more complete deletion in the packaging signal |
| 物种来源: | 人泡沫病毒前病毒DNA，HSRV2缺失变体 |
| 插入大小: | 2543 bp |
| 基因突变: | pCiGSΔψ是pCiGS-Gag表达质粒的更新版本，在包装信号中有更完整的缺失 |
| GenBank编号 | Y07724.1 Y07723.1 |
| 启动子 | CMV |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | NotI（克隆期间销毁） |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: |
pCiGSΔΨ was a gift from David Russell (Addgene plasmid # 108312 ; http://n2t.net/addgene:108312 ; RRID:Addgene_108312)
|
| 参考文献部分: | Transduction of long-term and mobilized peripheral blood-derived NOD/SCID repopulating cells by foamy virus vectors. Josephson NC, Trobridge G, Russell DW. Hum Gene Ther. 2004 Jan;15(1):87-92. 10.1089/10430340460732481 PubMed 14965380 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCiGS-Delta-Psi 5956 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Foamy virus helper plasmid expressing Gag, driven by a CMV promoter.
Ampicillin resistant..
ACCESSION .
VERSION .
KEYWORDS pCiGS-Delta-Psi
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5956)
AUTHORS Josephson NC, Trobridge G, Russell DW
TITLE Transduction of long-term and mobilized peripheral blood-derived
NOD/SCID repopulating cells by foamy virus vectors.
JOURNAL Hum Gene Ther. 2004 Jan;15(1):87-92.
PUBMED 14965380
REFERENCE 2 (bases 1 to 5956)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5956
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 27..330
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 331..534
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 485..505
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 531..555
/label=LNCX
/note="Human CMV promoter, forward primer"
intron 672..768
/label=SV40 intron
/note="modified SV40 intron with splice donor and acceptor
sites"
regulatory 839..848
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
polyA_signal 3095..3229
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3119..3138)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 3173..3192
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(3340..3356)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3340..3356)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(3353..3375)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 3364..3380
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3388..3418)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3433..3454
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(3571..3588)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3742..4330)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3822..3841)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4501..5361)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5124..5143
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5362..5466)
/gene="bla"
/label=AmpR promoter
primer_bind 5534..5552
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(5590..5612)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 5712..5731
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 5925..5947
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 5939..5956
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 5940..5956
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 gaattcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
601 cgggaccgat ccagcctccg gactctagag gatccggtac tcgaggaact gaaaaaccag
661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg gcccgcccaa cgacggccgc
841 caccatggct tcaggaagta atgttgaaga atatgaactt gatgttgaag ctctggttgt
901 aattttaaga gatagaaata taccaagaaa tcctttacat ggagaagtta taggtcttcg
961 ccttactgaa ggatggtggg gacaaattga gagatttcag atggtacgtc taatattaca
1021 aaatgatgat aatgaacctt tacagagacc tagatatgag gtaatacaac gagctgtaaa
1081 ccctcataca atgtttatga tatcaggacc attagctgaa cttcaattag cctttcagga
1141 tttagattta cctgaaggtc cattgaggtt tggtccattg gcaaatggac attatgttca
1201 aggagatcct tatagtagtt cttacagacc agtaacaatg gccgaaacag cccaaatgac
1261 tagagatgaa ctggaagatg ttcttaatac tcaaagtgaa atagaaattc aaatgataaa
1321 tttattggag ttgtatgaag ttgaaactag agctcttaga agacaattag ctgagagatc
1381 tagtacaggg caaggaggaa tatccccagg agctcctcgt tctcgaccac cagtaagcag
1441 cttctcaggg ttaccaagtt tgccctctat acctgggatt catcccaggg caccttcacc
1501 tccaagggca acttctactc cgggaaatat tccttggagt ttaggagatg ataacccacc
1561 ttcatctagt tttcctggac cctctcaacc tcgtgtttct ttccatccgg gaaatccttt
1621 tgttgaagaa gaaggtcata gacctagatc ccagtctaga gaaaggagaa gagaaattct
1681 tcctgctcct gtaccgtcag cacctcctat gattcagtat ataccagtac cacctccacc
1741 accgattggc acggttatac ctattcagca tatcagatct gtaactggag agcctcctag
1801 aaacccaaga gaaataccaa tttggctagg acgaaatgct cctgctatag atggagtgtt
1861 ccctgttaca acaccggatc taagatgcag aataattaat gctatactag gaggaaatat
1921 tgggctatca ttaacccctg gagactgttt aacatgggac tcagcagtag ccaccttatt
1981 tattagaacc catggaactt ttccaatgca tcagcttgga aatgtaataa aaggcatagt
2041 tgatcaagaa ggagtggcaa cagcatatac tttgggaatg atgctttctg gacaaaatta
2101 tcaattagtt tctggaataa ttagaggata tttgcctgga caagctgtag taactgcatt
2161 acaacagcgt ttagaccaag aaatagatga tcaaacaaga gcagagactt ttattcaaca
2221 tctaaatgct gtatatgaaa ttttaggcct taatgccaga ggacaaagta tacgtgcttc
2281 agtgactcct caaccccgac catccagagg tagaggtcga ggtcaaaata cttctagacc
2341 ctctcaagga ccagctaata gcgggcgggg acgacagcgc cctgcttctg gtcaaagcaa
2401 cagaggatct agtactcaga atcaaaatca agataattta aatcaaggag gatataatct
2461 tcgaccccgt acttaccaac ctcaaaggta cggaggagga cgtggacgaa gatggaacga
2521 taatactaac aatcaagagt ccagaccatc agatcaaggt tctcaaactc ctaggccaaa
2581 tcaagcaggc tctggggtgc gtggcaatca gtcacaaact cccagaccag ctgctggtcg
2641 cggaggaaga ggtaaccaca accgaaacca acgatcatcc ggtgctggtg actcacgcgc
2701 tgtcaatacc gtgacacaga gtgccacgtc ctccacagat gaatcctctt cagctgttac
2761 agccgcttcc ggcggagatc aaagggacta aattgttagc ccactgggat tcaggggcaa
2821 caataacttg tattcctgaa agttttttag aagatgaaca acctattaaa aagactttaa
2881 taaaaacaat tcatggagaa aaacaacaaa atgtttatta tgtaaccttt aaagttaaag
2941 gaagaaaagt ggaagcagaa gtgatagctt ctccttatga gtatattttg ctgtcgccaa
3001 cagatgttcc ttggttaaca cagcaaccac ttcagttaac aattttagtt cctcttcaag
3061 aatatcaaga gaaaatctta attgcggccg cggggatcca gacatgataa gatacattga
3121 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
3181 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
3241 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt cggatcctct
3301 agagtcgacc tgcaggcatg caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
3361 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
3421 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
3481 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
3541 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
3601 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
3661 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
3721 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
3781 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
3841 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
3901 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
3961 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
4021 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
4081 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
4141 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
4201 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
4261 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
4321 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
4381 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
4441 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
4501 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
4561 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
4621 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
4681 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
4741 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
4801 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
4861 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
4921 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
4981 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
5041 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
5101 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
5161 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
5221 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
5281 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
5341 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
5401 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
5461 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
5521 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
5581 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
5641 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
5701 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
5761 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
5821 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
5881 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
5941 taaaacgacg gccagt
//
