| 质粒名称: | pEB-C5 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 28213 |
| 存储实验室: | Linzhao Cheng |
|
载体骨架基本信息 |
|
| 载体名称: | pCEP4 |
| 载体出品公司: | invitrogen |
| 空载体大小: | 10200 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 潮霉素 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Oct4, Sox2, Klf4, c-Myc, Lin28 |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 7612 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pEB-C5 was a gift from Linzhao Cheng (Addgene plasmid # 28213 ; http://n2t.net/addgene:28213 ; RRID:Addgene_28213) |
| 参考文献部分: | Efficient human iPS cell derivation by a non-integrating plasmid from blood cells with unique epigenetic and gene expression signatures. Chou BK, Mali P, Huang X, Ye Z, Dowey SN, Resar LM, Zou C, Zhang YA, Tong J, Cheng L. Cell Res. 2011 Jan 18. ():. 10.1038/cr.2011.12 PubMed 21243013 |
买家导航
pEBC5载体基本信息
pEBC5载体图谱质粒图谱和多克隆位点信息
pEBC5载体简介
pEBC5载体序列
LOCUS pEB-C5 16768 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION 5 reprogramming factors (OCT4, SOX2, KLF4, c-MYC and LIN28) are
expressed as a single poly-cistronic unit.
ACCESSION .
VERSION .
KEYWORDS pEB-C5
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 16768)
AUTHORS Chou BK, Mali P, Huang X, Ye Z, Dowey SN, Resar LM, Zou C, Zhang YA,
Tong J, Cheng L
TITLE Efficient human iPS cell derivation by a non-integrating plasmid
from blood cells with unique epigenetic and gene expression
signatures.
JOURNAL Cell Res. 2011 Jan 18. ():.
PUBMED 21243013
REFERENCE 2 (bases 1 to 16768)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..16768
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 305..508
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 459..479
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 505..529
/label=LNCX
/note="Human CMV promoter, forward primer"
enhancer 570..949
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 951..1226
/label=chicken beta-actin promoter
intron 1227..2243
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 2167..2189
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 2251..2270
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
CDS 3381..3434
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 4410..4463
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 7299..7352
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
polyA_signal 8002..8136
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8026..8045)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 8080..8099
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(8279..8301)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(12483..12501)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 12569..12673
/gene="bla"
/label=AmpR promoter
CDS 12674..13534
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(12892..12911)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 13705..14293
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 14194..14213
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
promoter 14724..14869
/label=HSV TK promoter
/note="herpes simplex virus thymidine kinase promoter"
CDS 14912..15949
/codon_start=1
/product="hygromycin B phosphotransferase"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
MGEAN"
primer_bind complement(15947..15966)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 15991..16039
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
enhancer 16693..304
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
ORIGIN
1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
181 aagtccgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
241 catgacctta cgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
301 catggtgatg cggttttggc agtacaccaa tgggcgtgga tagcggtttg actcacgggg
361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
421 ggactttcca aaatgtcgta ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt
481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatct ctagaagctg
541 ggtaccagct gctagcatgg gcccatctcg acattgatta ttgactagtt attaatagta
601 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
661 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
721 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
781 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
841 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
901 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag
961 ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt
1021 atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg ggcgcgccag
1081 gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca
1141 atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct
1201 ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc
1261 gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg
1321 tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc
1381 ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg
1441 gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct
1501 ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca
1561 gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg
1621 ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt
1681 cggtcgggct gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt
1741 cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc
1801 ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc cggggrgggc tcgggggagg
1861 ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc
1921 ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc
1981 cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg
2041 ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc
2101 tccctctcca gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca
2161 gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc
2221 atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc
2281 attttggcaa agaattcaga tccaccatgg ctggacacct ggcttcagac ttcgccttct
2341 cacccccacc aggtgggggt gatgggtcag cagggctgga gccgggctgg gtggattctc
2401 gaacctggct aagcttccaa gggcctccag gtgggcctgg aatcggacca ggctcagagg
2461 tattggggat ctccccatgt ccgcccgcat acgagttctg cggagggatg gcatactgtg
2521 gacctcaggt tggactgggc ctagtccccc aagttggcgt ggagactttg cagcctgagg
2581 gccaggcagg agcacgagtg gaaagcaact cagagggaac ctcctctgag ccctgtgccg
2641 accgccccaa tgccgtgaag ttggagaagg tggaaccaac tcccgaggag tcccaggaca
2701 tgaaagccct gcagaaggag ctagaacagt ttgccaagct gctgaagcag aagaggatca
2761 ccttggggta cacccaggcc gacgtggggc tcaccctggg cgttctcttt ggaaaggtgt
2821 tcagccagac caccatctgt cgcttcgagg ccttgcagct cagccttaag aacatgtgta
2881 agctgcggcc cctgctggag aagtgggtgg aggaagccga caacaatgag aaccttcagg
2941 agatatgcaa atcggagacc ctggtgcagg cccggaagag aaagcgaact agcattgaga
3001 accgtgtgag gtggagtctg gagaccatgt ttctgaagtg cccgaagccc tccctacagc
3061 agatcactca catcgccaat cagcttgggc tagagaagga tgtggttcga gtatggttct
3121 gtaaccggcg ccagaagggc aaaagatcaa gtattgagta ttcccaacga gaagagtatg
3181 aggctacagg gacacctttc ccaggggggg ctgtatcctt tcctctgccc ccaggtcccc
3241 actttggcac cccaggctat ggaagccccc acttcaccac actctactca gtcccttttc
3301 ctgagggcga ggcctttccc tctgttcccg tcactgctct gggctctccc atgcattcaa
3361 acggcggcgg gtccggagga gagggcagag gaagtcttct aacatgcggt gacgtggagg
3421 agaatcctgg cccaatgtat aacatgatgg agacggagct gaagccgccg ggcccgcagc
3481 aagcttcggg gggcggcggc ggaggaggca acgccacggc ggcggcgacc ggcggcaacc
3541 agaagaacag cccggaccgc gtcaagaggc ccatgaacgc cttcatggta tggtcccggg
3601 ggcagcggcg taagatggcc caggagaacc ccaagatgca caactcggag atcagcaagc
3661 gcctgggcgc ggagtggaaa cttttgtccg agaccgagaa gcggccgttc atcgacgagg
3721 ccaagcggct gcgcgctctg cacatgaagg agcacccgga ttataaatac cggccgcggc
3781 ggaaaaccaa gacgctcatg aagaaggata agtacacgct tcccggaggc ttgctggccc
3841 ccggcgggaa cagcatggcg agcggggttg gggtgggcgc cggcctgggt gcgggcgtga
3901 accagcgcat ggacagctac gcgcacatga acggctggag caacggcagc tacagcatga
3961 tgcaggagca gctgggctac ccgcagcacc cgggcctcaa cgctcacggc gcggcacaga
4021 tgcaaccgat gcaccgctac gacgtcagcg ccctgcagta caactccatg accagctcgc
4081 agacctacat gaacggctcg cccacctaca gcatgtccta ctcgcagcag ggcacccccg
4141 gtatggcgct gggctccatg ggctctgtgg tcaagtccga ggccagctcc agcccccccg
4201 tggttacctc ttcctcccac tccagggcgc cctgccaggc cggggacctc cgggacatga
4261 tcagcatgta cctccccggc gccgaggtgc cggagcccgc tgcgcccagt agactgcaca
4321 tggcccagca ctaccagagc ggcccggtgc ccggcacggc cattaacggc acactgcccc
4381 tgtcgcacat gggcggcggg tccggaggag agggcagagg aagtcttcta acatgcggtg
4441 acgtggagga gaatcctggc ccaatggctg tcagcgacgc tctgctcccg tccttctcca
4501 cgttcgcgtc cggcccggcg ggaagggaga agacactgcg tccagcaggt gccccgacta
4561 accgttggcg tgaggaactc tctcacatga agcgacttcc cccacttccc ggccgcccct
4621 acgacctggc ggcgacggtg gccacagacc tggagagtgg cggagctggt gcagcttgca
4681 gcagtaacaa cccggccctc ctagcccgga gggagaccga ggagttcaac gacctcctgg
4741 acctagactt tatcctttcc aactcgctaa cccaccagga atcggtggcc gccaccgtga
4801 ccacctcggc gtcagcttca tcctcgtctt ccccagcgag cagcggccct gccagcgcgc
4861 cctccacctg cagcttcagc tatccgatcc gggccggggg tgacccgggc gtggctgcca
4921 gcaacacagg tggagggctc ctctacagcc gagaatctgc gccacctccc acggccccct
4981 tcaacctggc ggacatcaat gacgtgagcc cctcgggcgg cttcgtggct gagctcctgc
5041 ggccggagtt ggacccagta tacattccgc cacagcagcc tcagccgcca ggtggcgggc
5101 tgatgggcaa gtttgtgctg aaggcgtctc tgaccacccc tggcagcgag tacagcagcc
5161 cttcggtcat cagtgttagc aaaggaagcc cagacggcag ccaccccgtg gtagtggcgc
5221 cctacagcgg tggcccgccg cgcatgtgcc ccaagattaa gcaagaggcg gtcccgtcct
5281 gcacggtcag ccggtcccta gaggcccatt tgagcgctgg accccagctc agcaacggcc
5341 accggcccaa cacacacgac ttccccctgg ggcggcagct ccccaccagg actaccccta
5401 cactgagtcc cgaggaactg ctgaacagca gggactgtca ccctggcctg cctcttcccc
5461 caggattcca tccccatccg gggcccaact accctccttt cctgccagac cagatgcagt
5521 cacaagtccc ctctctccat tatcaagagc tcatgccacc gggttcctgc ctgccagagg
5581 agcccaagcc aaagagggga agaaggtcgt ggccccggaa aagaacagcc acccacactt
5641 gtgactatgc aggctgtggc aaaacctata ccaagagttc tcatctcaag gcacacctgc
5701 gaactcacac aggcgagaaa ccttaccact gtgactggga cggctgtggg tggaaattcg
5761 cccgctccga tgaactgacc aggcactacc gcaaacacac agggcaccgg ccctttcagt
5821 gccagaagtg cgacagggcc ttttccaggt cggaccacct tgccttacac atgaagaggc
5881 actttggcag cggaaaacag ctgttgaatt ttgaccttct caagttggcg ggagacgtgg
5941 agtccaaccc agggcccgtc gagatgcccc tcaacgtgaa cttcaccaac aggaactatg
6001 acctcgacta cgactccgta cagccctatt tcatctgcga cgaggaagag aatttctatc
6061 accagcaaca gcagagcgag ctgcagccgc ccgcgcccag tgaggatatc tggaagaaat
6121 tcgagctgct tcccaccccg cccctgtccc cgagccgccg ctccgggctc tgctctccat
6181 cctatgttgc ggtcgctacg tccttctccc caagggaaga cgatgacggc ggcggtggca
6241 acttctccac cgccgatcag ctggagatga tgaccgagtt acttggagga gacatggtga
6301 accagagctt catctgcgat cctgacgacg agaccttcat caagaacatc atcatccagg
6361 actgtatgtg gagcggtttc tcagccgctg ccaagctggt ctcggagaag ctggcctcct
6421 accaggctgc gcgcaaagac agcaccagcc tgagccccgc ccgcgggcac agcgtctgct
6481 ccacctccag cctgtacctg caggacctca ccgccgccgc gtccgagtgc attgacccct
6541 cagtggtctt tccctacccg ctcaacgaca gcagctcgcc caaatcctgt acctcgtccg
6601 attccacggc cttctctcct tcctcggact cgctgctgtc ctccgagtcc tccccacggg
6661 ccagccctga gcccctagtg ctgcatgagg agacaccgcc caccaccagc agcgactctg
6721 aagaagagca agaagatgag gaagaaattg atgtggtgtc tgtggagaag aggcaaaccc
6781 ctgccaagag gtcggagtcg ggctcatctc catcccgagg ccacagcaaa cctccgcaca
6841 gcccactggt cctcaagagg tgccacgtct ccactcacca gcacaactac gccgcacccc
6901 cctccacaag gaaggactat ccagctgcca agagggccaa gttggacagt ggcagggtcc
6961 tgaagcagat cagcaacaac cgcaagtgct ccagccccag gtcctcagac acggaggaaa
7021 acgacaagag gcggacacac aacgtcttgg aacgtcagag gaggaacgag ctgaagcgca
7081 gcttttttgc cctgcgtgac cagatccctg aattggaaaa caacgaaaag gcccccaagg
7141 tagtgatcct caaaaaagcc accgcctaca tcctgtccat tcaagcagac gagcacaagc
7201 tcacctctga aaaggactta ttgaggaaac gacgagaaca gttgaaacac aaactcgaac
7261 agcttcgaaa ctctggtgca ggcggcgggt ccggaggaga gggcagagga agtcttctaa
7321 catgcggtga cgtggaggag aatcctggcc caatgggctc ggtgtccaac cagcagtttg
7381 caggtggctg cgccaaggca gcggagaagg cgccagagga ggcgccgcct gacgcggccc
7441 gagcggcaga cgagccgcag ctgctgcacg gggccggcat ctgtaagtgg ttcaacgtgc
7501 gcatggggtt cggcttcctg tctatgaccg cccgcgccgg ggtcgcgctc gaccccccgg
7561 tggacgtctt tgtgcaccag agcaagctgc acatggaagg gttccgaagc ctcaaggagg
7621 gtgaggcggt ggagttcacc tttaagaagt ctgccaaggg tctggaatcc atccgtgtca
7681 ctggccctgg tggtgtgttc tgtattggaa gtgagcggcg gccaaagggg aagaacatgc
7741 agaagcgaag atccaaagga gacaggtgct acaactgcgg tgggctagac catcatgcca
7801 aggaatgcaa gctgccaccc cagcccaaga agtgccactt ttgccaaagc atcaaccata
7861 tggtggcctc gtgtccactg aaggcccagc agggccccag ttctcaggga aagcctgcct
7921 acttccggga ggaagaggaa gagatccaca gccctgccct gctcccagaa gcccagaatt
7981 gactcgaggc cggcaaggcc ggatccagac atgataagat acattgatga gtttggacaa
8041 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
8101 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
8161 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
8221 tgtggtatgg ctgattatga tccggctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
8281 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
8341 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaggt
8401 cgactctaga ggatcgatcc ccgccccgga cgaactaaac ctgactacga catctctgcc
8461 ccttcttcgc ggggcagtgc atgtaatccc ttcagttggt tggtacaact tgccaactgg
8521 gccctgttcc acatgtgaca cgggggggga ccaaacacaa aggggttctc tgactgtagt
8581 tgacatcctt ataaatggat gtgcacattt gccaacactg agtggctttc atcctggagc
8641 agactttgca gtctgtggac tgcaacacaa cattgccttt atgtgtaact cttggctgaa
8701 gctcttacac caatgctggg ggacatgtac ctcccagggg cccaggaaga ctacgggagg
8761 ctacaccaac gtcaatcaga ggggcctgtg tagctaccga taagcggacc ctcaagaggg
8821 cattagcaat agtgtttata aggccccctt gttaacccta aacgggtagc atatgcttcc
8881 cgggtagtag tatatactat ccagactaac cctaattcaa tagcatatgt tacccaacgg
8941 gaagcatatg ctatcgaatt agggttagta aaagggtcct aaggaacagc gatatctccc
9001 accccatgag ctgtcacggt tttatttaca tggggtcagg attccacgag ggtagtgaac
9061 cattttagtc acaagggcag tggctgaaga tcaaggagcg ggcagtgaac tctcctgaat
9121 cttcgcctgc ttcttcattc tccttcgttt agctaataga ataactgctg agttgtgaac
9181 agtaaggtgt atgtgaggtg ctcgaaaaca aggtttcagg tgacgccccc agaataaaat
9241 ttggacgggg ggttcagtgg tggcattgtg ctatgacacc aatataaccc tcacaaaccc
9301 cttgggcaat aaatactagt gtaggaatga aacattctga atatctttaa caatagaaat
9361 ccatggggtg gggacaagcc gtaaagactg gatgtccatc tcacacgaat ttatggctat
9421 gggcaacaca taatcctagt gcaatatgat actggggtta ttaagatgtg tcccaggcag
9481 ggaccaagac aggtgaacca tgttgttaca ctctatttgt aacaagggga aagagagtgg
9541 acgccgacag cagcggactc cactggttgt ctctaacacc cccgaaaatt aaacggggct
9601 ccacgccaat ggggcccata aacaaagaca agtggccact cttttttttg aaattgtgga
9661 gtgggggcac gcgtcagccc ccacacgccg ccctgcggtt ttggactgta aaataagggt
9721 gtaataactt ggctgattgt aaccccgcta accactgcgg tcaaaccact tgcccacaaa
9781 accactaatg gcaccccggg gaatacctgc ataagtaggt gggcgggcca agataggggc
9841 gcgattgctg cgatctggag gacaaattac acacacttgc gcctgagcgc caagcacagg
9901 gttgttggtc ctcatattca cgaggtcgct gagagcacgg tgggctaatg ttgccatggg
9961 tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc
10021 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
10081 atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc
10141 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc
10201 atatgctatc ctcatgcata tacagtcagc atatgatacc cagtagtaga gtgggagtgc
10261 tatcctttgc atatgccgcc acctcccaag ggggcgtgaa ttttcgctgc ttgtcctttt
10321 cctgctggtt gctcccattc ttaggtgaat ttaaggaggc caggctaaag ccgtcgcatg
10381 tctgattgct caccaggtaa atgtcgctaa tgttttccaa cgcgagaagg tgttgagcgc
10441 ggagctgagt gacgtgacaa catgggtatg cccaattgcc ccatgttggg aggacgaaaa
10501 tggtgacaag acagatggcc agaaatacac caacagcacg catgatgtct actggggatt
10561 tattctttag tgcgggggaa tacacggctt ttaatacgat tgagggcgtc tcctaacaag
10621 ttacatcact cctgcccttc ctcaccctca tctccatcac ctccttcatc tccgtcatct
10681 ccgtcatcac cctccgcggc agccccttcc accataggtg gaaaccaggg aggcaaatct
10741 actccatcgt caaagctgca cacagtcacc ctgatattgc aggtaggagc gggctttgtc
10801 ataacaaggt ccttaatcgc atccttcaaa acctcagcaa atatatgagt ttgtaaaaag
10861 accatgaaat aacagacaat ggactccctt agcgggccag gttgtgggcc gggtccaggg
10921 gccattccaa aggggagacg actcaatggt gtaagacgac attgtggaat agcaagggca
10981 gttcctcgcc ttaggttgta aagggaggtc ttactacctc catatacgaa cacaccggcg
11041 acccaagttc cttcgtcggt agtcctttct acgtgactcc tagccaggag agctcttaaa
11101 ccttctgcaa tgttctcaaa tttcgggttg gaacctcctt gaccacgatg ctttccaaac
11161 caccctcctt ttttgcgcct gcctccatca ccctgacccc ggggtccagt gcttgggcct
11221 tctcctgggt catctgcggg gccctgctct atcgctcccg ggggcacgtc aggctcacca
11281 tctgggccac cttcttggtg gtattcaaaa taatcggctt cccctacagg gtggaaaaat
11341 ggccttctac ctggaggggg cctgcgcggt ggagacccgg atgatgatga ctgactactg
11401 ggactcctgg gcctcttttc tccacgtcca cgacctctcc ccctggctct ttcacgactt
11461 ccccccctgg ctctttcacg tcctctaccc cggcggcctc cactacctcc tcgaccccgg
11521 cctccactac ctcctcgacc ccggcctcca ctgcctcctc gaccccggcc tccacctcct
11581 gctcctgccc ctcctgctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgct
11641 cctgcccctc ctgcccctcc tgctcctgcc cctcctgccc ctcctgcccc ctcctgcccc
11701 tcctgcccct cctcctgctc ctgcccctcc tcctgctcct gcccctcctg ctcctgcccc
11761 tcccgctcct gctcctgctc ctgttccacc gtgggtccct ttgcagccaa tgcaacttgg
11821 acgtttttgg ggtctccgga caccatctct atgtcttggc cctgatcctg agccgcccgg
11881 ggctcctggt cttccgcctc ctcgtcctcg tcctcttccc cgtcctcgtc catggttatc
11941 accccctctt ctttgaggtc cactgccgcc ggagccttct ggtccagatg tgtctccctt
12001 ctctcctagg ccatttccag gtcctgtacc tggcccctcg tcagacatga ttcacactaa
12061 aagagatcaa tagacatctt tattagacga cgctcagtga atacagggag tgcagactcc
12121 tgccccctcc aacagccccc ccaccctcat ccccttcatg gtcgctgtca gacagatcca
12181 ggtctgaaaa ttccccatcc tccgaaccat cctcgtcctc atcaccaatt actcgcagcc
12241 cggaaaactc ccgctgaaca tcctcaagat ttgcgtcctg agcctcaagc caggcctcaa
12301 attcctcgtc cccctttttg ctggacggta gggatgggga ttctcgggac ccctcctctt
12361 cctcttcaag gtcaccagac agagatgcta ctggggcaac ggaagaaaag ctgggtgcgg
12421 cctgtgagga tcagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa
12481 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
12541 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
12601 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
12661 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
12721 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
12781 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
12841 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
12901 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
12961 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
13021 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
13081 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
13141 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
13201 tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact
13261 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
13321 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
13381 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
13441 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
13501 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
13561 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
13621 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
13681 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
13741 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
13801 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
13861 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
13921 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
13981 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
14041 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
14101 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
14161 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
14221 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
14281 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
14341 ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg
14401 catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt
14461 cgcgaacgcc agcaagacgt agcccagcgc gtcggccccg agatgcgccg cgtgcggctg
14521 ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt
14581 ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc
14641 cctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat
14701 atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg tcattggcga
14761 attcgaacac gcagatgcag tcggggcggc gcggtccgag gtccacttcg catattaagg
14821 tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaac agcgtcaaca
14881 gcgtgccgca gatcccgggg ggcaatgaga tatgaaaaag cctgaactca ccgcgacgtc
14941 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
15001 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
15061 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
15121 cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg
15181 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
15241 tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac
15301 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
15361 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
15421 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
15481 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
15541 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
15601 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
15661 gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg
15721 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
15781 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
15841 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
15901 ccgacgcccc agcactcgtc cggatcggga gatgggggag gctaactgaa acacggaagg
15961 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
16021 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
16081 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
16141 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
16201 atagccactg gccccgtggg ttagggacgg ggtcccccat ggggaatggt ttatggttcg
16261 tgggggttat tattttgggc gttgcgtggg gtcaggtcca cgactggact gagcagacag
16321 acccatggtt tttggatggc ctgggcatgg accgcatgta ctggcgcgac acgaacaccg
16381 ggcgtctgtg gctgccaaac acccccgacc cccaaaaacc accgcgcgga tttctggcgt
16441 gccaagctag tcgaccaatt ctcatgtttg acagcttatc atcgcagatc cgggcaacgt
16501 tgttgccatt gctgcaggcg cagaactggt aggtatggaa gatctataca ttgaatcaat
16561 attggcaatt agccatatta gtcattggtt atatagcata aatcaatatt ggctattggc
16621 cattgcatac gttgtatcta tatcataata tgtacattta tattggctca tgtccaatat
16681 gaccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
16741 tagttcatag cccatatatg gagttccg
//
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