| 质粒名称: | pCAG-Eco |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 35617 |
| 存储实验室: | Arthur Nienhuis, Patrick Salmon |
|
载体骨架基本信息 |
|
| 载体名称: | pCAG |
| 载体出品公司: | -- |
| 空载体大小: | 4700 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Top10 |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Ecotropic MLV envelope |
| 物种来源: | -- |
| 插入大小: | 2000 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pCAG-Eco was a gift from Arthur Nienhuis & Patrick Salmon (Addgene plasmid # 35617 ; http://n2t.net/addgene:35617 ; RRID:Addgene_35617) |
买家导航
pCAGEco载体基本信息
pCAGEco载体图谱质粒图谱和多克隆位点信息
pCAGEco载体简介
pCAGEco载体序列
LOCUS pCAG-Eco 7059 bp ds-DNA circular SYN 16-AUG-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pCAG-Eco
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7059)
TITLE Pseudotyping Envelopes
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7059)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 16, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7059
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 4..383
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
primer_bind 1570..1592
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
misc_feature 1633..2007
/label=pol region
/note="Moloney murine leukemia virus (MMLV) pol region
containing the splice acceptor site"
primer_bind 1945..1961
/label=pBMN 5'
/note="MMLV sequence, for inserts in pBMN retroviral
vector"
primer_bind complement(4080..4099)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_signal 4145..4200
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(4199..4218)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(4561..4577)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4561..4577)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4574..4596)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4585..4601
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4609..4639)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4654..4675
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 4734..4929
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 4780..4915
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4842..4861
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
polyA_signal 4935..5069
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4972..4991)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5026..5045
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(5137..5154)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5308..5896)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5388..5407)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6067..6927)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6690..6709
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6928..7032)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
481 cgatgggggc gggggggggg ggggggcggg gcggggcgag gcggagaggt gcggcggcag
541 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc
601 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc
661 cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac
721 aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac
781 ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt
841 gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg
901 gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc
961 gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg
1021 aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg
1081 cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg
1141 cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt
1201 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg
1261 aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt
1321 gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctgtgcgg
1381 agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg
1441 gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc
1501 ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg gggggacggg
1561 gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tacgcaacac gccgggcccc
1621 catggcctca ccccatatga gatcttatat ggggcacccc cgccccttgt aaacttccct
1681 gaccctgaca tgacaagagt tactaacagc ccctctctcc aagctcactt acaggctctc
1741 tacttagtcc agcacgaagt ctggagacct ctggcggcag cctaccaaga acaactggac
1801 cgaccggtgg tacctcaccc ttaccgagtc ggcgacacag tgtgggtccg ccgacaccag
1861 actaagaacc tagaacctcg ctggaaagga ccttacacag tcctgctgac cacccccacc
1921 gccctcaaag tagacggcat cgcagcttgg atacacgccg cccacgtgaa ggctgccgac
1981 cccgggggtg gaccatcctc tagactgaca tggcgcgttc aacgctctca aaacccctta
2041 aaaataaggt taacccgcga ggccccctaa tccccttaat tcttctgatg ctcagagggg
2101 tcagtactgc ttcgcccggc tccagtcctc atcaagtcta taatatcacc tgggaggtaa
2161 ccaatggaga tcgggagacg gtatgggcaa cttctggcaa ccaccctctg tggacctggt
2221 ggcctgacct taccccagat ttatgtatgt tagcccacca tggaccatct tattgggggc
2281 tagaatatca atcccctttt tcttctcccc cggggccccc ttgttgctca gggggcagca
2341 gcccaggctg ttccagagac tgcgaagaac ctttaacctc cctcacccct cggtgcaaca
2401 ctgcctggaa cagactcaag ctagaccaga caactcataa atcaaatgag ggattttatg
2461 tttgccccgg gccccaccgc ccccgagaat ccaagtcatg tgggggtcca gactccttct
2521 actgtgccta ttggggctgt gagacaaccg gtagagctta ctggaagccc tcctcatcat
2581 gggatttcat cacagtaaac aacaatctca cctctgacca ggctgtccag gtatgcaaag
2641 ataataagtg gtgcaacccc ttagttattc ggtttacaga cgccgggaga cgggttactt
2701 cctggaccac aggacattac tggggcttac gtttgtatgt ctccggacaa gatccagggc
2761 ttacatttgg gatccgactc agataccaaa atctaggacc ccgcgtccca atagggccaa
2821 accccgttct ggcagaccaa cagccactct ccaagcccaa acctgttaag tcgccttcag
2881 tcaccaaacc acccagtggg actcctctct cccctaccca acttccaccg gcgggaacgg
2941 aaaataggct gctaaactta gtagacggag cctaccaagc cctcaacctc accagtcctg
3001 acaaaaccca agagtgctgg ttgtgtctag tagcgggacc cccctactac gaaggggttg
3061 ccgtcctggg tacctactcc aaccatacct ctgctccagc caactgctcc gtggcctccc
3121 aacacaagtt gaccctgtcc gaagtgaccg gacagggact ctgcatagga gcagttccca
3181 aaacacatca ggccctatgt aataccaccc agacaagcag tcgagggtcc tattatctag
3241 ttgcccctac aggtaccatg tgggcttgta gtaccgggct tactccatgc atctccacca
3301 ccatactgaa ccttaccact gattattgtg ttcttgtcga actctggcca agagtcacct
3361 atcattcccc cagctatgtt tacggcctgt ttgagagatc caaccgacac aaaagagaac
3421 cggtgtcgtt aaccctggcc ctattattgg gtggactaac catgggggga attgccgctg
3481 gaataggaac agggactact gctctaatgg ccactcagca attccagcag ctccaagccg
3541 cagtacagga tgatctcagg gaggttgaaa aatcaatctc taacctagaa aagtctctca
3601 cttccctgtc tgaagttgtc ctacagaatc gaaggggcct agacttgtta tttctaaaag
3661 aaggagggct gtgtgctgct ctaaaagaag aatgttgctt ctatgcggac cacacaggac
3721 tagtgagaga cagcatggcc aaattgagag agaggcttaa tcagagacag aaactgtttg
3781 agtcaactca aggatggttt gagggactgt ttaacagatc cccttggttt accaccttga
3841 tatctaccat tatgggaccc ctcattgtac tcctaatgat tttgctcttc ggaccctgca
3901 ttcttaatcg attagtccaa tttgttaaag acaggatatc agtggtccag gctctagttt
3961 tgactcaaca atatcaccag ctgaagccta tagagtacga gccatagttc tgagcgccgg
4021 tcgctaccat taccagttgg tctggtgtca aaaataataa taaccgggca aacggcagat
4081 ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct
4141 ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca
4201 ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag
4261 agtttggcaa catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt
4321 catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact
4381 tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa
4441 ttttccttac atgttttact agccagattt ttcctcctct cctgactact cccagtcata
4501 gctgtccctc ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg
4561 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
4621 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
4681 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tccgcatctc
4741 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
4801 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
4861 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
4921 ttttgcaaaa agctaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
4981 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
5041 tcatcaatgt atcttatcat gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg
5101 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
5161 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
5221 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
5281 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
5341 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
5401 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
5461 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
5521 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
5581 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
5641 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
5701 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
5761 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
5821 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
5881 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
5941 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
6001 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
6061 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
6121 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
6181 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
6241 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
6301 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
6361 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
6421 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
6481 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
6541 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
6601 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
6661 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
6721 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
6781 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
6841 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
6901 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
6961 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
7021 aggggttccg cgcacatttc cccgaaaagt gccacctgg
//
