质粒名称: | pUDP004 |
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出品公司: | Addgene |
目录编号: | 101165 |
存储实验室: | Jean-Marc Daran |
载体骨架基本信息 |
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载体名称: | not applicable |
载体出品公司: | -- |
空载体大小: | -- |
完整质粒大小: | -- |
载体修饰: | -- |
载体类型: | 酵母表达,CRISPR |
筛选标记: | amdS(赋予乙酰胺生长的乙酰胺酶基因) |
载体抗性: | 氨苄霉素 |
生长温度: | 37 ℃ |
宿主菌: | DH5a |
拷贝数: | 高拷贝 |
插入基因信息 |
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插入基因名称: | -- |
物种来源: | -- |
插入大小: | -- |
基因突变: | -- |
GenBank编号 | -- |
启动子: | ScTDH3 |
标签/融合蛋白: | -- |
克隆方法: | Gibson 克隆 |
5’克隆位点: | -- |
3’克隆位点: | -- |
5’测序引物 | -- |
3’测序引物 | -- |
文献引用方法 |
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材料和方法部分: | pUDP004 was a gift from Jean-Marc Daran (Addgene plasmid # 101165 ; http://n2t.net/addgene:101165 ; RRID:Addgene_101165) |
参考文献部分: | CRISPR-Cas9 mediated gene deletions in lager yeast Saccharomyces pastorianus. de Vries ARG, de Groot PA, van den Broek M, Daran JG. Microb Cell Fact. 2017 Dec 5;16(1):222. doi: 10.1186/s12934-017-0835-1. 10.1186/s12934-017-0835-1 [pii] PubMed 29207996 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pUDP004 10664 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION E. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV). ACCESSION . VERSION . KEYWORDS pUDP004 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10664) AUTHORS de Vries ARG, de Groot PA, van den Broek M, Daran JG TITLE CRISPR-Cas9 mediated gene deletions in lager yeast Saccharomyces pastorianus. JOURNAL Microb Cell Fact. 2017 Dec 5;16(1):222. doi: 10.1186/s12934-017-0835-1. PUBMED 29207996 REFERENCE 2 (bases 1 to 10664) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10664 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 2574..2597 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS 2601..2621 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 2622..6722 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTYKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSITHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" CDS 6726..6746 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" promoter 7665..8331 /gene="S. cerevisiae TDH3" /label=GAP promoter /note="promoter for glyceraldehyde-3-phosphate dehydrogenase" primer_bind 8201..8224 /label=GPDpro-F /note="S. cerevisiae GPD promoter, forward primer" primer_bind complement(8393..8411) /label=CYC1 /note="CYC1 transcription termination signal, reverse primer" CDS complement(8845..9705) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGTRVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9468..9487 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9706..9810) /gene="bla" /label=AmpR promoter rep_origin 9913..10501 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 10402..10421 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 gacatggagg cccagaatac cctccttgac agtcttgacg tgcgcagctc aggggcatga 61 tgtgactgtc gcccgtacat ttagcccata catccccatg tataatcatt tgcatccata 121 cattttgatg gccgcacggc gcgaagcaaa aattacggct cctcgctgca gacctgcgag 181 cagggaaacg ctcccctcac agacgcgttg aattgtcccc acgccgcgcc cctgtagaga 241 aatataaaag gttaggattt gccactgagg ttcttctttc atatacttcc ttttaaaatc 301 ttgctaggat acagttctca catcacatcc gaacataaac aaggatccat gccacaatct 361 tgggaagaat tggctgctga caagagagct agattggcta agactatccc agacgaatgg 421 aaggttcaaa ctttgccagc tgaagactct gttatcgact tcccaaagaa gtctggtatc 481 ttgtctgaag ctgaattgaa gatcactgaa gcttctgctg ctgacttggt ttctaagttg 541 gctgctggtg aattgacttc tgttgaagtt actttggctt tctgtaagag agctgctatc 601 gctcaacaat tgactaactg tgctcacgaa ttcttcccag acgctgcttt ggctcaagct 661 agagaattgg acgaatacta cgctaagcac aaaagacctg ttggtccatt gcacggtttg 721 ccaatctctt tgaaggacca attgagagtt aagggttacg aaacttctat gggttacatc 781 tcttggttga acaagtacga cgaaggtgac tctgttttga ctactatgtt gagaaaggct 841 ggtgctgttt tctacgttaa gacttctgtt ccacaaactt tgatggtttg tgaaactgtt 901 aacaacatca tcggtagaac tgttaaccca agaaacaaga actggtcttg tggtggttct 961 tctggtggtg aaggtgctat cgttggtatc agaggtggtg ttatcggtgt tggtactgac 1021 atcggtggtt ctatcagagt tccagctgct ttcaacttct tgtacggttt aagaccatct 1081 cacggtagat tgccatacgc taagatggct aactctatgg aaggtcaaga aactgtacac 1141 tctgttgttg gtccaatcac tcactctgtt gaagacttga gattgttcac taagtctgtt 1201 ttgggtcaag aaccatggaa gtacgactct aaggttatcc caatgccatg gagacaatct 1261 gaatctgaca tcatcgcttc taagatcaag aacggtggtt tgaacatcgg ttactacaac 1321 ttcgacggta acgttttgcc acacccacca atcttgagag gtgttgaaac tactgttgct 1381 gctttggcta aggctggtca cactgttact ccatggactc catacaagca cgacttcggt 1441 cacgacttga tctctcacat ctacgctgct gacggttctg ctgacgttat gagagacatc 1501 tctgcttctg gtgaaccagc tatcccaaac atcaaggact tgttgaaccc aaacatcaag 1561 gctgttaaca tgaacgaatt gtgggacact cacttgcaaa agtggaacta ccaaatggaa 1621 tacttggaaa agtggagaga agctgaagaa aaggctggta aggaattgga cgctatcatc 1681 gctccaatca ctccaactgc tgctgttaga cacgaccaat tcagatacta cggttacgct 1741 tctgttatca acttgttgga cttcacttct gttgttgttc cagttacttt cgctgacaag 1801 aacatcgaca agaagaacga atctttcaag gctgtttctg aattggacgc tttggttcaa 1861 gaagaatacg acccagaagc ttaccacggt gctccagttg ctgttcaagt tatcggtaga 1921 agattgtctg aagaaagaac tttggctatc gctgaagaag ttggtaagtt gttgggtaac 1981 gttgttactc cataactcga ggacaataaa aagattcttg ttttcaagaa cttgtcattt 2041 gtatagtttt tttatattgt agttgttcta ttttaatcaa atgttagcgt gatttatatt 2101 ttttttcgcc tcgacatcat ctgcccagat gcgaagttaa gtgcgcagaa agtaatatca 2161 tgcgtcaatc gtatgtgaat gctggtcgct atactggagc tctcgagaac cctcaccttt 2221 cgagaggacg atgcccgtgt ctaaatgatt cgaccagcct aagaatgttc aacaatctat 2281 aatcagtcca tagtcaacaa gagccctcaa agttgatatc gtttagtgat ctccctcgag 2341 atgagcggag gatggttgct ttttggggag ccctaagtgg tacgtgcggc gagcgggatg 2401 tttccccatt aggcaaagga cgaccgggta acccctcgag aaaaaaaatt tttcgccgct 2461 aatctggtgt tatataaagc tccccctgct cctggaattt ttttccttgt caactcacac 2521 cggaaatcaa ggcatttcat tctgagtagt tcttaaaaac ataatcaaca atggattata 2581 aagatgacga tgacaaacct ccaaaaaaga agagaaaggt cgataagaaa tactcaatag 2641 gcttagatat cggcacaaat agcgtcggat gggcggtgat cactgatgaa tataaggttc 2701 cgtctaaaaa gttcaaggtt ctgggaaata cagaccgcca cagtatcaaa aaaaatctta 2761 taggggctct tttatttgac agtggagaga cagcggaagc gactcgtctc aaacggacag 2821 ctcgtagaag gtatacacgt cggaagaatc gtatttgtta tctacaggag attttttcaa 2881 atgagatggc gaaagtagat gatagtttct ttcatcgact tgaagagtct tttttggtgg 2941 aagaagacaa gaagcatgaa cgtcatccta tttttggaaa tatagtagat gaagttgctt 3001 atcatgagaa atatccaact atctatcatc tgcgaaaaaa attggtagat tctacttata 3061 aagcggattt gcgcttaatc tatttggcct tagcgcatat gattaagttt cgtggtcatt 3121 ttttgattga gggagattta aatcctgata atagtgatgt ggacaaacta tttatccagt 3181 tggtacaaac ctacaatcaa ttatttgaag aaaaccctat taacgcaagt ggagtagatg 3241 ctaaagcgat tctttctgca cgattgagta aatcaagacg attagaaaat ctcattgctc 3301 agctccccgg tgagaagaaa aatggcttat ttgggaatct cattgctttg tcattgggtt 3361 tgacccctaa ttttaaatca aattttgatt tggcagaaga tgctaaatta cagctttcaa 3421 aagatactta cgatgatgat ttagataatt tattggcgca aattggagat caatatgctg 3481 atttgttttt ggcagctaag aatttatcag atgctatttt actttcagat atcctaagag 3541 taaatactga aataactaag gctcccctat cagcttcaat gattaaacgc tacgatgaac 3601 atcatcaaga cttgactctt ttaaaagctt tagttcgaca acaacttcca gaaaagtata 3661 aagaaatctt ttttgatcaa tcaaaaaacg gatatgcagg ttatattgat gggggagcta 3721 gccaagaaga attttataaa tttatcaaac caattttaga aaaaatggat ggtactgagg 3781 aattattggt gaaactaaat cgtgaagatt tgctgcgcaa gcaacggacc tttgacaacg 3841 gctctattac ccatcaaatt cacttgggtg agctgcatgc tattttgaga agacaagaag 3901 acttttatcc atttttaaaa gacaatcgtg agaagattga aaaaatcttg acttttcgaa 3961 ttccttatta tgttggtcca ttggcgcgtg gcaatagtcg ttttgcatgg atgactcgga 4021 agtctgaaga aacaattacc ccatggaatt ttgaagaagt tgtcgataaa ggtgcttcag 4081 ctcaatcatt tattgaacgc atgacaaact ttgataaaaa tcttccaaat gaaaaagtac 4141 taccaaaaca tagtttgctt tatgagtatt ttacggttta taacgaattg acaaaggtca 4201 aatatgttac tgaaggaatg cgaaaaccag catttctttc aggtgaacag aagaaagcca 4261 ttgttgattt actcttcaaa acaaatcgaa aagtaaccgt taagcaatta aaagaagatt 4321 atttcaaaaa aatagaatgt tttgatagtg ttgaaatttc aggagttgaa gatagattta 4381 atgcttcatt aggtacctac catgatttgc taaaaattat taaagataaa gattttttgg 4441 ataatgaaga aaatgaagat atcttagagg atattgtttt aacattgacc ttatttgaag 4501 atagggagat gattgaggaa agacttaaaa catatgctca cctctttgat gataaggtga 4561 tgaaacagct taaacgtcgc cgttatactg gttggggacg tttgtctcga aaattgatta 4621 atggtattag ggataagcaa tctggcaaaa caatattaga ttttttgaaa tcagatggtt 4681 ttgccaatcg caattttatg cagctgatcc atgatgatag tttgacattt aaagaagaca 4741 ttcaaaaagc acaagtgtct ggacaaggcg atagtttaca tgaacatatt gcaaatttag 4801 ctggtagccc tgctattaaa aaaggtattt tacagactgt aaaagttgtt gatgaattgg 4861 tcaaagtaat ggggcggcat aagccagaaa atatcgttat tgaaatggca cgtgaaaatc 4921 agacaactca aaagggccag aaaaattcgc gagagcgtat gaaacgaatc gaagaaggta 4981 tcaaagaatt aggaagtcag attcttaaag agcatcctgt tgaaaatact caattgcaaa 5041 atgaaaagct ctatctctat tatctccaaa atggaagaga catgtatgtg gaccaagaat 5101 tagatattaa tcgtttaagt gattatgatg tcgatcacat tgttccacaa agtttcctta 5161 aagacgattc aatagacaat aaggtcttaa cgcgttctga taaaaatcgt ggtaaatcgg 5221 ataacgttcc aagtgaagaa gtagtcaaaa agatgaaaaa ctattggaga caacttctaa 5281 acgccaagtt aatcactcaa cgtaagtttg ataatttaac gaaagctgaa cgtggaggtt 5341 tgagtgaact tgataaagct ggttttatca aacgccaatt ggttgaaact cgccaaatca 5401 ctaagcatgt ggcacaaatt ttggatagtc gcatgaatac taaatacgat gaaaatgata 5461 aacttattcg agaggttaaa gtgattacct taaaatctaa attagtttct gacttccgaa 5521 aagatttcca attctataaa gtacgtgaga ttaacaatta ccatcatgcc catgatgcgt 5581 atctaaatgc cgtcgttgga actgctttga ttaagaaata tccaaaactt gaatcggagt 5641 ttgtctatgg tgattataaa gtttatgatg ttcgtaaaat gattgctaag tctgagcaag 5701 aaataggcaa agcaaccgca aaatatttct tttactctaa tatcatgaac ttcttcaaaa 5761 cagaaattac acttgcaaat ggagagattc gcaaacgccc tctaatcgaa actaatgggg 5821 aaactggaga aattgtctgg gataaagggc gagattttgc cacagtgcgc aaagtattgt 5881 ccatgcccca agtcaatatt gtcaagaaaa cagaagtaca gacaggcgga ttctccaagg 5941 agtcaatttt accaaaaaga aattcggaca agcttattgc tcgtaaaaaa gactgggatc 6001 caaaaaaata tggtggtttt gatagtccaa cggtagctta ttcagtccta gtggttgcta 6061 aggtggaaaa agggaaatcg aagaagttaa aatccgttaa agagttacta gggatcacaa 6121 ttatggaaag aagttccttt gaaaaaaatc cgattgactt tttagaagct aaaggatata 6181 aggaagttaa aaaagactta atcattaaac tacctaaata tagtcttttt gagttagaaa 6241 acggtcgtaa acggatgctg gctagtgccg gagaattaca aaaaggaaat gagctggctc 6301 tgccaagcaa atatgtgaat tttttatatt tagctagtca ttatgaaaag ttgaagggta 6361 gtccagaaga taacgaacaa aaacaattgt ttgtggagca gcataagcat tatttagatg 6421 agattattga gcaaatcagt gaattttcta agcgtgttat tttagcagat gccaatttag 6481 ataaagttct tagtgcatat aacaaacata gagacaaacc aatacgtgaa caagcagaaa 6541 atattattca tttatttacg ttgacgaatc ttggagctcc cgctgctttt aaatattttg 6601 atacaacaat tgatcgtaaa cgatatacgt ctacaaaaga agttttagat gccactctta 6661 tccatcaatc catcactggt ctttatgaaa cacgcattga tttgagtcag ctaggaggtg 6721 accctccaaa aaagaagaga aaggtctgag cggttttgta taactaaata atattggaaa 6781 ctaaatacga atacccaaat tttttatcta aattttgccg aaagattaaa atctgcagag 6841 atatccgaaa caggtaaatg gatgtttcaa tccctgtagt cagtcaggaa cccatattat 6901 attacagtat tagtcgccgc ttaggcacgc ctttaattag caaaatcaaa ccttaagtgc 6961 atatgccgta taagggaaac tcaaagaact ggcatcgcaa aaatgaaaac atacgttgaa 7021 actacggcaa aggattggtc agatcgcttc atacagggaa agttcggcat caacatcttt 7081 ggataatatc agaatgagaa agaacagata cgcagtacgt tttttggtga gctctttgca 7141 cttctttagt tctttccatc aatatcagtt ttttaaactt ttaggactaa aagtgatgtt 7201 taacttcaaa atgtttaaaa ttttgttctt cccgacgttc attaagaata ctaatacact 7261 ttaataatta ttttaatatt ttgttctaaa taatgacttt taattaaaaa agataaaata 7321 taaaaacatc ataataactc accagaggtt aagaacaaaa aaacaaatta gatatctgct 7381 aatccaatat agttaaatca atctttcctt ggtataatgg gtatattaca tatatttcaa 7441 ggaccgacac tcctaccaaa tatctaaaat ttaccatatt aacataacat gtatataaac 7501 gtcaaatcat aatcagcact aacgtctcac ggatcgtata tgccgtagcg acaatctaag 7561 aactatgcga ggacacgcta gggccaaggc aaaaagattc cttgattacg taagggagtt 7621 agaatcattt tgaataaaaa acacgctttt tcagttcgag tttatcatta tcaatactgc 7681 catttcaaag aatacgtaaa taattaatag tagtgatttt cctaacttta tttagtcaaa 7741 aaattagcct tttaattctg ctgtaacccg tacatgccca aaataggggg cgggttacac 7801 agaatatata acatcgtagg tgtctgggtg aacagtttat tcttggcatc cactaaatat 7861 aatggagccc gctttttaag ctggcatcca gaaaaaaaaa gaatcccagc accaaaatat 7921 tgttttcttc accaaccatc agttcatagg tccattctct tagcgcaact acagagaaca 7981 ggggcacaaa caggcaaaaa acgggcacaa cctcaatgga gtgatgcaac ctgcctggag 8041 taaatgatga cacaaggcaa ttgacccacg catgtatcta tctcattttc ttacaccttc 8101 tattaccttc tgctctctct gatttggaaa aagctgaaaa aaaaggttga aaccagttcc 8161 ctgaaattat tcccctactt gactaataag tatataaaga cggtaggtat tgattgtaat 8221 tctgtaaatc tatttcttaa acttcttaaa ttctactttt atagttagtc ttttttttag 8281 ttttaaaaca ccaagaactt agtttcgaat aaacacacat aaacaaacaa acgagacctg 8341 cggaggtctc cacaggcccc ttttcctttg tcgatatcat gtaattagtt atgtcacgct 8401 tacattcacg ccctcccccc acatccgctc taaccgaaaa ggaaggagtt agacaacctg 8461 aagtctaggt ccctatttat ttttttatag ttatgttagt attaagaacg ttatttatat 8521 ttcaaatttt tctttttttt ctgtacaaac gcgtgtacgc atgtaacatt atactgaaaa 8581 ccttgcttga gaaggttttg ggacaagctt acgtctcacg gatcgtatat gccgtagcga 8641 caatctaaga actatgcgag gacacgctag cagctgtatt cacgtagacg gataggtata 8701 gccagacatc agcagcatac ttcgggaacc gtaggccacc gagcggccgc gattatcaaa 8761 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 8821 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 8881 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 8941 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgggacc cacgctcacc 9001 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 9061 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 9121 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 9181 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 9241 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 9301 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 9361 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 9421 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 9481 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 9541 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgtac ccaactgatc 9601 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 9661 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 9721 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 9781 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgtcat 9841 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat 9901 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 9961 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 10021 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt 10081 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 10141 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 10201 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 10261 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 10321 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 10381 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcc 10441 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 10501 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat 10561 gttctttcct gcgttatccc ctgattctgt ggataaccgt gcggactata tgtgaaggca 10621 tggctatggc acggcagaca ttccgccaga tcatcaatag gcac //
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