| 质粒名称: | pmGFP-ADAR1-p150 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 117927 |
| 存储实验室: | Kumiko Ui-Tei |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5428 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | ADAR1-p150 |
| 物种来源: | H. sapiens (human) |
| 基因突变: | NM_001111 NM_001111 |
| 启动子 | CMV |
| 标签/融合蛋白: | myc GFP（插入件上的N端子） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | HindIII (未破坏) |
| 3' 克隆位点: | XbaI(未破坏) |
| 5' 测序引物 | AAAGGGaagcttATGAATCCGCGGCAGGGGTATTCC |
| 3' 测序引物 | AAAGGGtctagaCTATACTGGGCAGAGATAAAAGTTC |
|
文献引用方法 |
|
| 材料和方法部分: | pmGFP-ADAR1-p150 was a gift from Kumiko Ui-Tei (Addgene plasmid # 117927 ; http://n2t.net/addgene:117927 ; RRID:Addgene_117927) |
| 参考文献部分: | Differential Binding of Three Major Human ADAR Isoforms to Coding and Long Non-Coding Transcripts. Galipon J, Ishii R, Suzuki Y, Tomita M, Ui-Tei K. Genes (Basel). 2017 Feb 11;8(2). pii: genes8020068. doi: 10.3390/genes8020068. 10.3390/genes8020068 PubMed 28208661 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pmGFP-ADAR1-p150 9801 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION ADAR1-p150 expression plasmid..
ACCESSION .
VERSION .
KEYWORDS pmGFP-ADAR1-p150
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9801)
AUTHORS Galipon J, Ishii R, Suzuki Y, Tomita M, Ui-Tei K
TITLE Differential Binding of Three Major Human ADAR Isoforms to Coding
and Long Non-Coding Transcripts.
JOURNAL Genes (Basel). 2017 Feb 11;8(2). pii: genes8020068. doi:
10.3390/genes8020068.
PUBMED 28208661
REFERENCE 2 (bases 1 to 9801)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9801
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 155..534
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 535..738
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 689..709
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 783..802
/label=T7
/note="T7 promoter, forward primer"
promoter 783..801
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 829..858
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 868..1584
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 868..1584
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(913..934)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1174..1193)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1521..1542
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(5312..5329)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5318..5542
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 5588..6016
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5675..5694)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5885..5906
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(6025..6045)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 6030..6359
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 6210..6345
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6272..6291
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 6426..7220
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(6480..6499)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 7090..7109
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 7394..7515
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7431..7450)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7485..7504
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(7564..7580)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7564..7580)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7577..7599)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7588..7604
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7612..7642)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7657..7678
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7795..7812)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7966..8554)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8046..8065)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8725..9585)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9348..9367
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9586..9690)
/gene="bla"
/label=AmpR promoter
primer_bind complement(9765..9784)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca
61 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg
121 ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa
181 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
241 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
301 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
361 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
421 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
481 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
541 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
601 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
661 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
721 gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa
781 attaatacga ctcactatag ggagacccaa gctggctagc ccaccatgga acaaaaactc
841 atctcagaag aggatctgtc cggcggcatg gtgagcaagg gcgaggagct gttcaccggg
901 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
961 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
1021 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
1081 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
1141 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
1201 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
1261 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
1321 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
1381 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
1441 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
1501 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
1561 ctcggcatgg acgagctgta caagtccggc ggcaagctta tgaatccgcg gcaggggtat
1621 tccctcagcg gatactacac ccatccattt caaggctatg agcacagaca gctcagatac
1681 cagcagcctg ggccaggatc ttcccccagt agtttcctgc ttaagcaaat agaatttctc
1741 aaggggcagc tcccagaagc accggtgatt ggaaagcaga caccgtcact gccaccttcc
1801 ctcccaggac tccggccaag gtttccagta ctacttgcct ccagtaccag aggcaggcaa
1861 gtggacatca ggggtgtccc caggggcgtg catctcggaa gtcaggggct ccagagaggg
1921 ttccagcatc cttcaccacg tggcaggagt ctgccacaga gaggtgttga ttgcctttcc
1981 tcacatttcc aggaactgag tatctaccaa gatcaggaac aaaggatctt aaagttcctg
2041 gaagagcttg gggaagggaa ggccaccaca gcacatgatc tgtctgggaa acttgggact
2101 ccgaagaaag aaatcaatcg agttttatac tccctggcaa agaagggcaa gctacagaaa
2161 gaggcaggaa cacccccttt gtggaaaatc gcggtctcca ctcaggcttg gaaccagcac
2221 agcggagtgg taagaccaga cggtcatagc caaggagccc caaactcaga cccgagtttg
2281 gaaccggaag acagaaactc cacatctgtc tcagaagatc ttcttgagcc ttttattgca
2341 gtctcagctc aggcttggaa ccagcacagc ggagtggtaa gaccagacag tcatagccaa
2401 ggatccccaa actcagaccc aggtttggaa cctgaagaca gcaactccac atctgccttg
2461 gaagatcctc ttgagttttt agacatggcc gagatcaagg agaaaatctg cgactatctc
2521 ttcaatgtgt ctgactcctc tgccctgaat ttggctaaaa atattggcct taccaaggcc
2581 cgagatataa atgctgtgct aattgacatg gaaaggcagg gggatgtcta tagacaaggg
2641 acaacccctc ccatatggca tttgacagac aagaagcgag agaggatgca aatcaagaga
2701 aatacgaaca gtgttcctga aaccgctcca gctgcaatcc ctgagaccaa aagaaacgca
2761 gagttcctca cctgtaatat acccacatca aatgcctcaa ataacatggt aaccacagaa
2821 aaagtggaga atgggcagga acctgtcata aagttagaaa acaggcaaga ggccagacca
2881 gaaccagcaa gactgaaacc acctgttcat tacaatggcc cctcaaaagc agggtatgtt
2941 gactttgaaa atggccagtg ggccacagat gacatcccag atgacttgaa tagtatccgc
3001 gcagcaccag gtgagtttcg agccatcatg gagatgccct ccttctacag tcatggcttg
3061 ccacggtgtt caccctacaa gaaactgaca gagtgccagc tgaagaaccc catcagcggg
3121 ctgttagaat atgcccagtt cgctagtcaa acctgtgagt tcaacatgat agagcagagt
3181 ggaccacccc atgaacctcg atttaaattc caggttgtca tcaatggccg agagtttccc
3241 ccagctgaag ctggaagcaa gaaagtggcc aagcaggatg cagctatgaa agccatgaca
3301 attctgctag aggaagccaa agccaaggac agtggaaaat cagaagaatc atcccactat
3361 tccacagaga aagaatcaga gaagactgca gagtcccaga cccccacccc ttcagccaca
3421 tccttctttt ctgggaagag ccccgtcacc acactgcttg agtgtatgca caaattgggg
3481 aactcctgcg aattccgtct cctgtccaaa gaaggccctg cccatgaacc caagttccaa
3541 tactgtgttg cagtgggagc ccaaactttc cccagtgtga gtgctcccag caagaaagtg
3601 gcaaagcaga tggccgcaga ggaagccatg aaggccctgc atggggaggc gaccaactcc
3661 atggcttctg ataaccagcc tgaaggtatg atctcagagt cacttgataa cttggaatcc
3721 atgatgccca acaaggtcag gaagattggc gagctcgtga gatacctgaa caccaaccct
3781 gtgggtggcc ttttggagta cgcccgctcc catggctttg ctgctgaatt caagttggtc
3841 gaccagtccg gacctcctca cgagcccaag ttcgtttacc aagcaaaagt tgggggtcgc
3901 tggttcccag ccgtctgcgc acacagcaag aagcaaggca agcaggaagc agcagatgcg
3961 gctctccgtg tcttgattgg ggagaacgag aaggcagaac gcatgggttt cacagaggta
4021 accccagtga caggggccag tctcagaaga actatgctcc tcctctcaag gtccccagaa
4081 gcacagccaa agacactccc tctcactggc agcaccttcc atgaccagat agccatgctg
4141 agccaccggt gcttcaacac tctgactaac agcttccagc cctccttgct cggccgcaag
4201 attctggccg ccatcattat gaaaaaagac tctgaggaca tgggtgtcgt cgtcagcttg
4261 ggaacaggga atcgctgtgt aaaaggagat tctctcagcc taaaaggaga aactgtcaat
4321 gactgccatg cagaaataat ctcccggaga ggcttcatca ggtttctcta cagtgagtta
4381 atgaaataca actcccagac tgcgaaggat agtatatttg aacctgctaa gggaggagaa
4441 aagctccaaa taaaaaagac tgtgtcattc catctgtata tcagcactgc tccgtgtgga
4501 gatggcgccc tctttgacaa gtcctgcagc gaccgtgcta tggaaagcac agaatcccgc
4561 cactaccctg tcttcgagaa tcccaaacaa ggaaagctcc gcaccaaggt ggagaacgga
4621 gaaggcacaa tccctgtgga atccagtgac attgtgccta cgtgggatgg cattcggctc
4681 ggggagagac tccgtaccat gtcctgtagt gacaaaatcc tacgctggaa cgtgctgggc
4741 ctgcaagggg cactgttgac ccacttcctg cagcccattt atctcaaatc tgtcacattg
4801 ggttaccttt tcagccaagg gcatctgacc cgtgctattt gctgtcgtgt gacaagagat
4861 gggagtgcat ttgaggatgg actacgacat ccctttattg tcaaccaccc caaggttggc
4921 agagtcagca tatatgattc caaaaggcaa tccgggaaga ctaaggagac aagcgtcaac
4981 tggtgtctgg ctgatggcta tgacctggag atcctggacg gtaccagagg cactgtggat
5041 gggccacgga atgaattgtc ccgggtctcc aaaaagaaca tttttcttct atttaagaag
5101 ctctgctcct tccgttaccg cagggatcta ctgagactct cctatggtga ggccaagaaa
5161 gctgcccgtg actacgagac ggccaagaac tacttcaaaa aaggcctgaa ggatatgggc
5221 tatgggaact ggattagcaa accccaggag gaaaagaact tttatctctg cccagtatag
5281 tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
5341 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
5401 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
5461 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
5521 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
5581 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
5641 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
5701 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
5761 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
5821 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
5881 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
5941 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
6001 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
6061 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
6121 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
6181 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
6241 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
6301 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
6361 agctcccggg agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt
6421 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
6481 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
6541 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
6601 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
6661 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
6721 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
6781 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
6841 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
6901 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
6961 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
7021 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
7081 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
7141 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
7201 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
7261 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
7321 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
7381 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
7441 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
7501 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
7561 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
7621 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
7681 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
7741 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
7801 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
7861 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
7921 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
7981 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
8041 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
8101 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
8161 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
8221 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
8281 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
8341 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
8401 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
8461 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
8521 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
8581 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
8641 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
8701 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
8761 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
8821 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
8881 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
8941 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
9001 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
9061 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
9121 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
9181 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
9241 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
9301 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
9361 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
9421 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
9481 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
9541 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
9601 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
9661 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
9721 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
9781 gccgcatagt taagccagta t
//
