| 质粒名称: | pMito-iRFP713 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 45465 |
| 存储实验室: | Vladislav Verkhusha |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 载体出品公司: | Clontech |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 4038 bp |
| 完整质粒大小: | 4989 bp |
| 载体修饰: | 人细胞色素c氧化酶亚基VIII（human cytochrome C oxidase subunit VIII） |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | iRFP713 |
| 物种来源: | 光合细菌 Rhodopseudomonas palustris |
| 插入大小: | 951 bp |
| 基因突变: | T2A, S13L, A92T, V104T, V114I, E161K, Y193K, F198Y, D202T, I203V, Y258F, A283V, K288T, N290Y relative to RpBphP2 |
| GenBank编号 | JN247409 |
| 启动子 | CMV |
| 标签/融合蛋白: | 人细胞色素c氧化酶亚基VIII（N端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AgeI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | pMito-iRFP713 was a gift from Vladislav Verkhusha (Addgene plasmid # 45465) |
| 参考文献部分: |
Near-infrared fluorescent proteins for multicolor in vivo imaging. Shcherbakova DM, Verkhusha VV. Nat Methods. 2013 Jun 16. doi: 10.1038/nmeth.2521. 10.1038/nmeth.2521 PubMed 23770755 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pMito-iRFP713 4989 bp circular DNA SYN
DEFINITION pMito-iRFP713
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pMito-iRFP713|
FEATURES Location/Qualifiers
source 1..4989
/organism="pMito-iRFP713"
/mol_type="other DNA"
promoter 10..562
/label="CMV_immearly_promoter"
misc_feature 65..352
/label="CAG_enhancer"
CDS complement(447..1034)
/label="ORF frame 2"
misc_feature 519..539
/label="CMV_fwd_primer"
promoter 520..589
/label="CMV_promoter"
CDS 597..1655
/label="ORF frame 3"
misc_feature 1868..1887
/label="EBV_rev_primer"
rep_origin complement(2037..2343)
/label="f1_origin"
promoter 2422..2450
/label="AmpR_promoter"
misc_feature complement(2516..2536)
/label="pBABE_3_primer"
misc_feature complement(2522..2737)
/label="SV40_enhancer"
promoter 2534..2802
/label="SV40_promoter"
rep_origin 2701..2778
/label="SV40_origin"
misc_feature 2763..2782
/label="SV40pro_F_primer"
CDS 2885..3679
/label="ORF frame 2"
gene 2888..3676
/label="NeoR/KanR"
/gene="NeoR/KanR"
CDS complement(3194..3730)
/label="ORF frame 3"
terminator 3854..4123
/label="TK_PA_terminator"
rep_origin 4271..4890
/label="pBR322_origin"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatgt
601 ccgtcctgac gccgctgctg ctgcggggct tgacaggctc ggcccggcgg ctcccagtgc
661 cgcgcgccaa gatccattcg ttgggggatc caccggtcgc caccatggcg gaaggatccg
721 tcgccaggca gcctgacctc ttgacctgcg acgatgagcc gatccatatc cccggtgcca
781 tccaaccgca tggactgctg ctcgccctcg ccgccgacat gacgatcgtt gccggcagcg
841 acaaccttcc cgaactcacc ggactggcga tcggcgccct gatcggccgc tctgcggccg
901 atgtcttcga ctcggagacg cacaaccgtc tgacgatcgc cttggccgag cccggggcgg
961 ccgtcggagc accgatcact gtcggcttca cgatgcgaaa ggacgcaggc ttcatcggct
1021 cctggcatcg ccatgatcag ctcatcttcc tcgagctcga gcctccccag cgggacgtcg
1081 ccgagccgca ggcgttcttc cgccgcacca acagcgccat ccgccgcctg caggccgccg
1141 aaaccttgga aagcgcctgc gccgccgcgg cgcaagaggt gcggaagatt accggcttcg
1201 atcgggtgat gatctatcgc ttcgcctccg acttcagcgg cgaagtgatc gcagaggatc
1261 ggtgcgccga ggtcgagtca aaactaggcc tgcactatcc tgcctcaacc gtgccggcgc
1321 aggcccgtcg gctctatacc atcaacccgg tacggatcat tcccgatatc aattatcggc
1381 cggtgccggt caccccagac ctcaatccgg tcaccgggcg gccgattgat cttagcttcg
1441 ccatcctgcg cagcgtctcg cccgtccatc tggaattcat gcgcaacata ggcatgcacg
1501 gcacgatgtc gatctcgatt ttgcgcggcg agcgactgtg gggattgatc gtttgccatc
1561 accgaacgcc gtactacgtc gatctcgatg gccgccaagc ctgcgagcta gtcgcccagg
1621 ttctggcctg gcagatcggc gtgatggaag agtgagcggc cgcgactcta gatcataatc
1681 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
1741 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
1801 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
1861 tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa
1921 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
1981 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
2041 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
2101 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
2161 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
2221 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
2281 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
2341 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
2401 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
2461 aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat
2521 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
2581 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
2641 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
2701 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
2761 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
2821 ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt
2881 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
2941 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
3001 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
3061 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
3121 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
3181 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
3241 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
3301 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
3361 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc
3421 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
3481 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
3541 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
3601 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
3661 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
3721 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
3781 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
3841 ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
3901 cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat
3961 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg
4021 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc
4081 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat
4141 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
4201 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
4261 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
4321 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
4381 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
4441 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
4501 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
4561 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
4621 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
4681 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
4741 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
4801 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
4861 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
4921 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
4981 gccatgcat
//
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